scholarly journals Prevalence and antibiotic resistance profile of Listeria spp. associated with seafoods from fish catchment areas in Kerala, India

2021 ◽  
Vol 14 (3) ◽  
pp. 777-783
Author(s):  
K. Vrinda Menon ◽  
B. Sunil ◽  
C. Latha
Keyword(s):  
Antibiotic Resistance ◽  
Antibiotic Sensitivity ◽  
Sensitivity Test ◽  
Isolation And Identification ◽  
Resistance Profile ◽  
Fresh Fish ◽  
Antibiotic Resistance Profile ◽  
Catchment Areas ◽  
Antibiotic Sensitivity Test ◽  
Serotype 4B

Background and Aim: Listeria monocytogenes is a ubiquitous, intracellular pathogen which has been implicated as a cause of several foodborne outbreaks. This study aimed to generate information on the prevalence and antibiotic resistance profile of Listeria species isolated from seafood. Materials and Methods: A total of 400 samples of fresh fish, 100 samples of dry fish and 200 samples each of crustaceans and mollusks were collected from the fish catchment areas. All the samples were subjected to isolation and identification of Listeria spp. by two-step enrichment in UVM broth and plating on selective agar media (PALCAM) and then subjected to molecular characterization. L. monocytogenes isolates obtained during the study were subjected to serotyping by multiplex polymerase chain reaction. The isolates were also subjected to antibiotic sensitivity test. Results: The prevalence of L. monocytogenes in seafoods in the present study was 0.55%. The isolates of L. monocytogenes were found to possess all virulence genes, namely, iap, hlyA, actA, prfA, plcA, and inlA. All the isolates belonged to serotype 4b. The occurrence of Listeria innocua was found to be more and was detected in 16.77% of seafoods samples. Antibiotic sensitivity test revealed that all isolates were resistant to cefixime but were sensitive to almost all other commonly used antibiotics. Conclusion: The presence of Listeria spp. in raw seafood samples augments the need for implementation of good hygienic practices during the handling and processing of seafoods to safeguard the health of the consumers.

scholarly journals Sensitivitas Antibiotik Terhadap Bakteri yang Diisolasi dari Ulkus Diabetika di RSUD Abepura, Kota Jayapura

2018 ◽  
Vol 6 (2) ◽  
pp. 53-59
Author(s):  
Sulistianingsih Sulistianingsih ◽  
Dirk Y.P. Runtuboi ◽  
Lucky V. Waworuntu
Keyword(s):  
Bacterial Infections ◽  
Healing Process ◽  
Antibiotic Sensitivity ◽  
Polymyxin B ◽  
Sensitivity Test ◽  
Wound Healing Process ◽  
Isolation And Identification ◽  
Diabetic Ulcer ◽  
Diabetic Ulcers ◽  
Antibiotic Sensitivity Test

Diabetic ulcer is a complication of Diabetes mellitus that is often found on the legs and easily develop into an infection. For the prevention of this symtomps, antibiotic is required to accelerate the wound healing process. The purpose of this study was to determine the bacteria that infect diabetic ulcers and antibiotic sensitivity test against the bacterial isolates in Abepura hospitals, Jayapura. This study used exploratory laboratory and was conducted at the Regional Health Laboratory (Labkesda) in Jayapura. The results of the isolation and identification showed that 6 species of bacteria have been found. The bacteria which most infected patients with diabetic ulcers in hospitals Abepura was Escherichia coli (38%). The result of antibiotic sensitivity test in hospitals Abepura ceftazidime therapy, showed that antibiotic sensitivity of Siprofloxacin therapy was only 16.7%. Imipenem choice of antibiotics had a sensitivity of 100% againts 6 types of bacteria that cause infections in patients with diabetic ulcers. Other choices of antibiotics which had a sensitivity of over 50% is Nitrofurantoin (83.3%), Gentamicin, Kanamycin and Polymyxin B and 66.7% respectively.Key words: Bacterial infections, diabetic ulcers, antibiotic sensitivity

A RETROSPECTIVE STUDY ON ANTIBIOTIC USAGE IN A TERTIARY CARE HOSPITAL

2020 ◽  
Vol 4 (6) ◽  
Author(s):  
Anna Joy ◽  
Aparna Anand ◽  
Arathy R Nath ◽  
Meera S Nair ◽  
Dr. K. G. Prasanth
Keyword(s):  
Retrospective Study ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Sensitivity Test ◽  
Actual Process ◽  
Care Hospital ◽  
Rational Use ◽  
Antibiotic Susceptibility Test ◽  
Antibiotic Sensitivity Test ◽  
Rational Use Of Antibiotics

Antibiotics are one of the most commonly prescribed drugs today. Rational use of antibiotics is therefore extremely important as their injudicious use can adversely affect the patient. Drug Utilization Evaluation (DUE) is a system of ongoing systematic criteria based evaluation of drug that will help to ensure that medicines are used appropriately. It is drug/disease specific and can be structured so that it will assess the actual process of prescribing, dispensing, or administration of drug. The retrospective study was conducted At Pk Das Institute of Medical Sciences, Palakkad, Kerala for a duration of 6 months (February 2017 - January 2018). A source of data includes Patient case sheets &medication charts, nursing charts, culture & sensitivity reports. The inclusion criterion includes Patients aged between 18- 80 year, prescribed with oral and parenteral antibiotics. . On analyzing the gender, male gender (n= 111, 55.5%) were higher in numbers as compared to female counterparts (n=89, 44.5%). In our study the majority of the patients prescribed with antibiotics were with the clinical assessment of COPD (n=39, 19.5%), UTI (n=37, 18.5%) and LRTI (n=28, 14%), Bronchial asthma (n=19, 9.5%) respectively. On analyzing the data based on antibiotic sensitivity test, antibiotic test were performed and followed in (n=64,32%)prescriptions and in (n=47,23.5%)prescriptions were test is not followed respectively. In (n=89,44.5%) prescriptions, antibiotic sensitivity test is not performed. On analysis of antibiotics prescribed, the most commonly prescribed antibiotics were cephalosporins, of these ceftriaxone was highly prescribed of all (n=95). The high percentage of antibiotic prescriptions may indicate a high probability of irrational use. This study also point out irrational use of antibiotics are more leading to resistance, misuse and serious problems. So certain strategies should be put forward to strengthen rational use of antibiotics. Keywords: Antibiotics, Antibiotic Susceptibility Test, Irrational use, Resistance

scholarly journals Comparison of Antibiotic Resistance Profile of Escherichia coli between Pristine and Human-Impacted Sites in a River

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 575
Author(s):  
Emi Nishimura ◽  
Masateru Nishiyama ◽  
Kei Nukazawa ◽  
Yoshihiro Suzuki
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Resistant Bacteria ◽  
Downstream Site ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Upstream Site ◽  
Resistance Profile ◽  
Antibiotic Resistance Profile ◽  
Significant Difference

Information on the actual existence of antibiotic-resistant bacteria in rivers where sewage, urban wastewater, and livestock wastewater do not load is essential to prevent the spread of antibiotic-resistant bacteria in water environments. This study compared the antibiotic resistance profile of Escherichia coli upstream and downstream of human habitation. The survey was conducted in the summer, winter, and spring seasons. Resistance to one or more antibiotics at upstream and downstream sites was on average 18% and 20%, respectively, and no significant difference was observed between the survey sites. The resistance rates at the upstream site (total of 98 isolated strains) to each antibiotic were cefazolin 17%, tetracycline 12%, and ampicillin 8%, in descending order. Conversely, for the downstream site (total of 89 isolated strains), the rates were ampicillin 16%, cefazolin 16%, and tetracycline 1% in descending order. The resistance rate of tetracycline in the downstream site was significantly lower than that of the upstream site. Furthermore, phylogenetic analysis revealed that many strains showed different resistance profiles even in the same cluster of the Pulsed-Field Gel Electrophoresis (PFGE) pattern. Moreover, the resistance profiles differed in the same cluster of the upstream and the downstream sites. In flowing from the upstream to the downstream site, it is plausible that E. coli transmitted or lacked the antibiotic resistance gene.

scholarly journals ANTIBIOTIC RESISTANCE PROFILING OF DAIRY WASTEWATER DEGRADING NATIVE EFFICIENT MICROBIAL ISOLATES

2017 ◽  
Vol 10 (7) ◽  
pp. 145
Author(s):  
Anupama Bhardwaj ◽  
Jagtar Singh ◽  
Sonia Chaman ◽  
Amit Joshi
Keyword(s):  
Antibiotic Resistance ◽  
Oxygen Demand ◽  
Dairy Wastewater ◽  
Organic Load ◽  
Diffusion Method ◽  
Resistance Profile ◽  
Antibiotic Resistance Profile ◽  
Surrounding Environment ◽  
Before And After ◽  
Microbial Isolates

Objective: The objective of this study is to make sure biotreatment process used for treatment of dairy wastewater (DWW) is safe for human and its surrounding environment; microbes were evaluated for their antibiotic resistance profile against commonly prescribed antibiotics. Methods: Microbes were isolated using spread plating and streaking method and used to treat DWW. Reduction in organic load in DWW was determined by comparing physicochemical parameters (PCP) of DWW before and after treatment process. After selection of efficient microbial isolates, they were evaluated for their antibiotic resistance profile using antibiotic disc diffusion method. Results: In this work, 53 microbes were isolated from DWW, and these microbial isolates were screened for DWW degradation capacity by analyzing PCP. Four microbial isolates E3, E5, E11 (bacterial isolates) and F5 (fungal isolate) showed highest reduction in chemical oxygen demand (COD), biological oxygen demand (BOD), and dissolved oxygen (DO) were selected for profound degradation of DWW under optimized conditions. Efficient four microbial isolates individually performed better under anaerobic conditions by showing maximum reduction 84%, 75%, and 77% in COD, BOD, and DO, respectively. After 72 hrs of antibiotic susceptibility testing, E3 strain had shown 100%, E5 90%, E11 70%, and F5 80% susceptibility to antibiotics. Conclusion: The present study concluded that four microbial isolates had the potential of reducing the organic load of DWW along with lessor or negligible adverse effect on human or its surrounding environment and they appear to be most promising strains for treatment of DWW. 

scholarly journals BACTERIOLOGICAL EXAMINATION OF PUS SAMPLE COLLECTED FROM A COW: A CASE STUDY

2016 ◽  
Vol 4 (12) ◽  
pp. 31-33
Author(s):  
Subha Ganguly
Keyword(s):  
Present Article ◽  
Antibiotic Sensitivity ◽  
Neck Region ◽  
Sensitivity Test ◽  
Bacteriological Examination ◽  
Antibiotic Sensitivity Test

The present article reports on the bacteriological examination and antibiotic sensitivity test of pus sample collected from an abscess at the neck region of a cow.

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