The topography and proliferative activity of cells immunoreactive to various growth factors in rat femoral bone tissues after experimental fracture and implantation of titanium implants with bioactive biodegradable coatings

2019 ◽  
Vol 30 (1) ◽  
pp. 85-95
Author(s):  
Sergei G. Kalinichenko ◽  
Natalya Yu Matveeva ◽  
Roman Ye Kostiv ◽  
Sergey S. Edranov
Keyword(s):  
Growth Factors ◽  
Proliferative Activity ◽  
Titanium Implants ◽  
Femoral Bone ◽  
Experimental Fracture ◽  
Biodegradable Coatings

Cytokines and Growth Factors Involved in the Osseointegration of Oral Titanium Implants Positioned Using Piezoelectric Bone Surgery Versus a Drill Technique: A Pilot Study in Minipigs

2007 ◽  
Vol 78 (4) ◽  
pp. 716-722 ◽  
Author(s):  
Giulio Preti ◽  
Germana Martinasso ◽  
Bruno Peirone ◽  
Roberto Navone ◽  
Carlo Manzella ◽  
...  
Keyword(s):  
Growth Factors ◽  
Pilot Study ◽  
Titanium Implants ◽  
Bone Surgery

scholarly journals Local application of concentrated trombocytes and the level of osseointegration of dental titanium implants: a histomorphometrical analysis

2006 ◽  
Vol 63 (8) ◽  
pp. 731-736 ◽  
Author(s):  
Zoran Lazic ◽  
Marija Bubalo ◽  
Smiljana Matic ◽  
Novak Stamatovic
Keyword(s):  
Growth Factors ◽  
Platelet Rich Plasma ◽  
Titanium Implants ◽  
Bone Implant ◽  
Lower Jaw ◽  
Interface Growth ◽  
Bone To Implant Contact ◽  
The Difference ◽  
The Right ◽  
Functional Loading

Background/aim: In order to achieve better, and, thus an increased bone-implant interface, growth factors have been used over the past few years. All growth factors considered have fundamental role in the growth and development of cells and tissues. Concentrated trombocytes from platelet-rich plasma (CT-PRP) are fraction of the blood. Thrombocytes contain a number of growth factors namely PDGF, TGF-?, IGF, VEGF and many others, which contribute to the achievement of the increased bone-implant interface, the increased stability of implants and the faster functional loading of implants. The aim of this study was to establish the effect of CT-PRP on bone implant interface. Methods. This experimental study included six dogs in which 24 BCT implants were inserted (4 implants per dog). On the left side of the lower jaw 2 implants were placed with CT-PRP, while on the right side the implants were placed without CT-PRP. The animals were sacrificed after 42, 70, and 98 days. The specimens were examined histomorphometrically, and analyzed 42, 70 and 98 days after the implant insertion. The contacts bone to implant in 16 zones for each analyzed implant were measured according to the established protocol. Results. Results obtained with histomorphometrical analysis imply the increased bone-to-implant contact by use CTPRP. The difference of the bone-to-implant contacts between these two groups of inserted implants has been particularly pronounced at six weeks after the implant insertion. Conclusion. According to the obtained results in the measurement of the level of osseointegration of the inserted implants, it should be advisable to use the CT-PRP method because it provides the higher level of osseointegration.

Autologous platelets as a source of proteins for healing and tissue regeneration

2004 ◽  
Vol 91 (01) ◽  
pp. 4-15 ◽  
Author(s):  
Eduardo Anitua ◽  
Isabel Andia ◽  
Bruno Ardanza ◽  
Paquita Nurden ◽  
Alan Nurden
Keyword(s):  
Growth Factors ◽  
Tissue Regeneration ◽  
Cosmetic Surgery ◽  
Tendon Repair ◽  
Fibrin Clot ◽  
Positive Influence ◽  
Titanium Implants ◽  
Therapeutic Effects ◽  
Active Metabolites ◽  
Time Required

SummaryPlatelets are known for their role in haemostasis where they help prevent blood loss at sites of vascular injury. To do this, they adhere, aggregate and form a procoagulant surface leading to thrombin generation and fibrin formation. Platelets also release substances that promote tissue repair and influence the reactivity of vascular and other blood cells in angiogenesis and inflammation. They contain storage pools of growth factors including PDGF, TGF-β and VEGF as well as cytokines including proteins such as PF4 and CD40L. Chemokines and newly synthesised active metabolites are also released. The fact that platelets secrete growth factors and active metabolites means that their applied use can have a positive influence in clinical situations requiring rapid healing and tissue regeneration. Their administration in fibrin clot or fibrin glue provides an adhesive support that can confine secretion to a chosen site. Additionally, the presentation of growth factors attached to platelets and/or fibrin may result in enhanced activity over recombinant proteins. Dental implant surgery with guided bone regeneration is one situation where an autologous platelet-rich clot clearly accelerates ossification after tooth extraction and/or around titanium implants. The end result is both marked reductions in the time required for implant stabilisation and an improved success rate. Orthopaedic surgery, muscle and/or tendon repair, reversal of skin ulcers, hole repair in eye surgery and cosmetic surgery are other situations where autologous platelets accelerate healing. Our aim is to review these advances and discuss the ways in which platelets may provide such unexpected beneficial therapeutic effects.

The effect of calcium phosphate biodegradable coatings of titanium implants on cell differentiation and apoptosis in rat bone tissue after experimental fracture

2020 ◽  
pp. 1-10
Author(s):  
Sergei Kalinichenko ◽  
Natalya Matveeva ◽  
Roman Kostiv ◽  
Sergey Edranov
Keyword(s):  
Stem Cells ◽  
Calcium Phosphate ◽  
Caspase 3 ◽  
Titanium Implants ◽  
Surrounding Tissue ◽  
Spatial Density ◽  
Experimental Fracture ◽  
Phosphate Coatings ◽  
Calcium Phosphate Coatings ◽  
Haversian Canals

BACKGROUND: The effectiveness of bone repair is determined by the balance of proliferative and destructive factors in the fracture union site. It can be enhanced by using various nanostructured materials possessing osteoinductive properties, in particular titanium implants with biodegradable calcium phosphate coatings. The effects of these coatings on the state of stem cells, their differentiation and distribution in the repair zone is unknown. OBJECTIVE : To study the dynamics of proliferation, differentiation, and apoptosis of stem cells after experimental fracture followed by implantation of titanium implants with calcium phosphate coatings. METHODS: The localization of proliferation (PCNA) and differentiation (CD44 and osteocalcin) factors and apoptotic molecules (MDM2, p53, caspase-3) was studied in a rat femoral fracture model with implant placement. Titanium implant screws with bioactive calcium phosphate and hydroxyapatite coatings formed by plasma electrolytic oxidation were used in the study. Experimental rats were arranged into three groups (15 animals per group): control group; rats implanted with uncoated implants; and rats implanted with coated implants. Control rats were subject to a similar fracture as experimental ones and were allowed to heal conservatively. Rats from all groups were sampled on days 7, 14, and 30 after injury. RESULTS: Low-differentiated PCNA-, osteocalcin-, and CD44-immunopositive cells were localized around the implant in the inner layer of the periosteum, layer of outer circumferential lamellae, and connective tissue lining of haversian canals. The spatial density of cells expressing the above proliferation and differentiation factors, as well as that of MDM2-immunoreactive cells, increased on day 7 and decreased by day 30 after injury. The spatial density of apoptotic cells reached the maximum on day 14 after injury. They were mainly found in the inner layer of the periosteum and outer circumferential lamellae. p53- and caspase-3-positive cells occurred on the surface of the concentric lamellae surrounding haversian canals and under the periosteum. Their spatial density decreased by day 30 after injury. CONCLUSIONS: Calcium phosphate coatings stimulate cell proliferation at early stages of fracture restoration and apoptotic cell death at later stages. Coating components may provide positional information guiding the differentiation of mesenchymal stromal cells. A change in the activity of apoptotic factors, osteocalcin, and CD44 is caused by gene induction in response to the diffusion of calcium phosphate compounds from coating to surrounding tissue.

scholarly journals Soft Tissue Attachment to Titanium Implants Coated with Growth Factors

2011 ◽  
Vol 15 (1) ◽  
pp. 53-63 ◽  
Author(s):  
Christopher Bates ◽  
Victor Marino ◽  
Nicola L. Fazzalari ◽  
P. Mark Bartold
Keyword(s):  
Growth Factors ◽  
Soft Tissue ◽  
Titanium Implants ◽  
Soft Tissue Attachment ◽  
Tissue Attachment

Osseointegration interface of calcified bone and endosseous implants

1992 ◽  
Vol 50 (2) ◽  
pp. 1096-1097
Author(s):  
K.E. Krizan ◽  
J.E. Laffoon ◽  
M.J. Buckley
Keyword(s):  
Structure And Function ◽  
Titanium Implants ◽  
En Bloc ◽  
Endosseous Implants ◽  
Ultrastructural Studies ◽  
The Past ◽  
Cacodylate Buffer ◽  
One Year ◽  
And Function

With increase use of tissue-integrated prostheses in recent years it is a goal to understand what is happening at the interface between haversion bone and bulk metal. This study uses electron microscopy (EM) techniques to establish parameters for osseointegration (structure and function between bone and nonload-carrying implants) in an animal model. In the past the interface has been evaluated extensively with light microscopy methods. Today researchers are using the EM for ultrastructural studies of the bone tissue and implant responses to an in vivo environment. Under general anesthesia nine adult mongrel dogs received three Brånemark (Nobelpharma) 3.75 × 7 mm titanium implants surgical placed in their left zygomatic arch. After a one year healing period the animals were injected with a routine bone marker (oxytetracycline), euthanized and perfused via aortic cannulation with 3% glutaraldehyde in 0.1M cacodylate buffer pH 7.2. Implants were retrieved en bloc, harvest radiographs made (Fig. 1), and routinely embedded in plastic. Tissue and implants were cut into 300 micron thick wafers, longitudinally to the implant with an Isomet saw and diamond wafering blade [Beuhler] until the center of the implant was reached.

Growth factors and the primary cilium in BALB/c 3T3 cells

1987 ◽  
Vol 45 ◽  
pp. 830-831
Author(s):  
R. W. Tucker ◽  
N. S. More ◽  
S. Jayaraman
Keyword(s):  
Growth Factors ◽  
Primary Cilium ◽  
Cultured Cells ◽  
Morphological Changes ◽  
Calcium Ionophore ◽  
Growth Stimulation ◽  
Calcium Ionophore A23187 ◽  
Ionophore A23187 ◽  
3T3 Cells ◽  
Microtubule Depolymerization

The mechanisms by which polypeptide growth factors Induce DNA synthesis in cultured cells is not understood, but morphological changes Induced by growth factors have been used as clues to Intracellular messengers responsible for growth stimulation. One such morphological change has been the transient disappearance of the primary cilium, a “9 + 0” cilium formed by the perinuclear centriole in interphase cells. Since calcium ionophore A23187 also produced both mitogenesis and ciliary changes, microtubule depolymerization might explain ciliary disappearance monitored by indirect immunofluorescence with anti-tubulin antibody. However, complete resorption and subsequent reformation of the primary cilium occurs at mitosis, and might also account for ciliary disappearance induced by growth factors. To settle this issue, we investigated the ultrastructure of the primary cilium using serial thin-section electron microscopy of quiescent BALB/c 3T3 cells before and after stimulation with serum.

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