Effect of Arthrospira powders from different producers on the formation of endothelial cell monolayers

Arthrospira platensis (AP) and some of its derived products have well-established biological activities as antioxidants or as agents to reduce cardiovascular disease risk factors. Furthermore, AP products have gained increasing importance as potential anti-cancer agents. However, the ingredients of the available products vary greatly with the origin, the type of production and processing, which could have significant consequences for their biological effects. Therefore, the composition and biological influence of five distinct AP powders, which were acquired commercially or produced at a public biotechnology institute, were investigated in regard to their endothelialization capacity using a cell impedance- (CI) based measurement method. The study revealed that the AP composition and especially the influence on HUVEC proliferation differed significantly between the five AP powders up to 109%. Thus, it could be shown that the method used allows the reliable detection of quantitative differences in biological effects of different AP preparations.

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Nutritional and health benefits of pulses

Applied Physiology Nutrition and Metabolism ◽

10.1139/apnm-2013-0557 ◽

2014 ◽

Vol 39 (11) ◽

pp. 1197-1204 ◽

Cited By ~ 97

Author(s):

Adriana N. Mudryj ◽

Nancy Yu ◽

Harold M. Aukema

Keyword(s):

Disease Risk ◽

Cardiovascular Disease Risk ◽

Dietary Recommendations ◽

Platelet Activity ◽

Randomized Controlled ◽

Anti Cancer ◽

Aging Populations ◽

Carcinogenic Effects ◽

Low Glycemic Index

Pulses (beans, peas, and lentils) have been consumed for at least 10 000 years and are among the most extensively used foods in the world. A wide variety of pulses can be grown globally, making them important both economically as well as nutritionally. Pulses provide protein and fibre, as well as a significant source of vitamins and minerals, such as iron, zinc, folate, and magnesium, and consuming half a cup of beans or peas per day can enhance diet quality by increasing intakes of these nutrients. In addition, the phytochemicals, saponins, and tannins found in pulses possess antioxidant and anti-carcinogenic effects, indicating that pulses may have significant anti-cancer effects. Pulse consumption also improves serum lipid profiles and positively affects several other cardiovascular disease risk factors, such as blood pressure, platelet activity, and inflammation. Pulses are high in fibre and have a low glycemic index, making them particularly beneficial to people with diabetes by assisting in maintaining healthy blood glucose and insulin levels. Emerging research examining the effect of pulse components on HIV and consumption patterns with aging populations indicates that pulses may have further effects on health. In conclusion, including pulses in the diet is a healthy way to meet dietary recommendations and is associated with reduced risk of several chronic diseases. Long-term randomized controlled trials are needed to demonstrate the direct effects of pulses on these diseases.

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Synthesis of Daidzein Glycosides, α-Tocopherol Glycosides, Hesperetin Glycosides by Bioconversion and Their Potential for Anti-Allergic Functional-Foods and Cosmetics

Molecules ◽

10.3390/molecules24162975 ◽

2019 ◽

Vol 24 (16) ◽

pp. 2975

Author(s):

Yuya Fujitaka ◽

Hiroki Hamada ◽

Daisuke Uesugi ◽

Atsuhito Kuboki ◽

Kei Shimoda ◽

...

Keyword(s):

Inhibitory Activity ◽

Water Solubility ◽

Biological Activities ◽

Biological Effects ◽

Physicochemical Stability ◽

Peritoneal Mast Cells ◽

Anti Cancer ◽

New Compounds ◽

Poor Absorption ◽

Anti Inflammation

Daidzein is a common isoflavone, having multiple biological effects such as anti-inflammation, anti-allergy, and anti-aging. α-Tocopherol is the tocopherol isoform with the highest vitamin E activity including anti-allergic activity and anti-cancer activity. Hesperetin is a flavone, which shows potent anti-inflammatory effects. These compounds have shortcomings, i.e., water-insolubility and poor absorption after oral administration. The glycosylation of bioactive compounds can enhance their water-solubility, physicochemical stability, intestinal absorption, and biological half-life, and improve their bio- and pharmacological properties. They were transformed by cultured Nicotiana tabacum cells to 7-β-glucoside and 7-β-gentiobioside of daidzein, and 3′- and 7-β-glucosides, 3′,7-β-diglucoside, and 7-β-gentiobioside of hesperetin. Daidzein and α-tocopherol were glycosylated by galactosylation with β-glucosidase to give 4′- and 7-β-galactosides of daidzein, which were new compounds, and α-tocopherol 6-β-galactoside. These nine glycosides showed higher anti-allergic activity, i.e., inhibitory activity toward histamine release from rat peritoneal mast cells, than their respective aglycones. In addition, these glycosides showed higher tyrosinase inhibitory activity than the corresponding aglycones. Glycosylation of daidzein, α-tocopherol, and hesperetin greatly improved their biological activities.

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Effects on mammalian cell transcriptome reveal a plant extract's medicinal properties: Potential anti-COVID-19 and anti-cancer properties of Sarcopoterium spinosum

10.21203/rs.3.rs-1250188/v1 ◽

2022 ◽

Author(s):

Valid Gahramanov ◽

Moria Oz ◽

Tzemach Aouizerat ◽

Mali Salmon-Divon ◽

Tovit Rosenzweig ◽

...

Keyword(s):

Mammalian Cell ◽

Plant Extract ◽

Biological Activities ◽

Biological Effects ◽

Gene Set Enrichment Analysis ◽

Functional Screening ◽

Pharmacological Activities ◽

Anti Cancer ◽

Medicinal Properties ◽

Sarcopoterium Spinosum

Abstract Plants with medicinal properties are usually identified based on traditional medicine knowledge or using low-throughput screens for specific pharmacological activities. Here, we suggest a different approach to uncover a range of pharmacological activities of a chosen plant extract without the need for functional screening. This tactic predicts biological activities of a plant extract based on pathway analysis of transcriptome changes caused by the extract in mammalian cell culture. In this work, we identified transcriptome changes after exposure of cultured cells to an extract of the medicinal plant Sarcopoterium spinosum. Gene Set Enrichment Analysis (GSEA) confirmed known anti-inflammatory and anti-cancer activities of the extract and predicted novel biological effects on oxidative phosphorylation and interferon pathways. Experimental validation of these pathways uncovered strong activation of autophagy, including mitophagy, and astounding protection from SARS-CoV-2 infection. Our study shows that gene expression analysis alone is insufficient for predicting biological effects since some of the changes reflect compensatory effects, and additional biochemical tests provide necessary corrections. In conclusion, this study defines the advantages and limitations of an approach towards predicting the biological and medicinal effects of plant extracts based on transcriptome changes caused by these extracts in mammalian cells.

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Cyclodextrin Complexation Improves the Solubility and Caco-2 Permeability of Chrysin

Materials ◽

10.3390/ma13163618 ◽

2020 ◽

Vol 13 (16) ◽

pp. 3618 ◽

Cited By ~ 1

Author(s):

Ferenc Fenyvesi ◽

Thi Le Phuong Nguyen ◽

Ádám Haimhoffer ◽

Ágnes Rusznyák ◽

Gábor Vasvári ◽

...

Keyword(s):

Biological Activities ◽

Biological Effects ◽

Phase Solubility ◽

Molar Ratios ◽

Solubility Study ◽

Permeability Enhancement ◽

Mtt Method ◽

Permeability Study ◽

Anti Cancer ◽

Poorly Soluble

Chrysin is a bioflavonoid that can be found in natural products such as honey and propolis, and it possesses several biological effects such as antioxidant, anti-inflammatory, and anti-cancer activity. However, it is poorly soluble in water, and its bioavailability is limited. The aim of this research is to investigate the chrysin solubilization capacity of different β-cylcodextrin derivatives and compare their biological activities. Chrysin was complexed with β-cyclodextrin (βCD), hydroxypropyl-β-, (HPBCD) sulfobutylether-β-, (SBECD), and randomly-methylated-β-cyclodextrin (RAMEB) by the lyophilization method in 1:1 and 1:2 molar ratios. The solubilities of the chrysin–cyclodextrin complexes were tested, and the solubilization abilities of cyclodextrins were studied by phase solubility experiments. The cytotoxicity of the complexes was measured by the MTT method, and the permeability enhancement was tested on Caco-2 monolayers. The solubility study showed that the complexes formed with RAMEB had the highest solubility in water. The phase solubility experiments confirmed the strongest interaction between RAMEB and chrysin. In the viability test, none of the complexes showed cytotoxicity up to 100 µM concentration. The permeability study revealed that both at 1:1 and 1:2 ratios, the RAMEB complexes were the most effective to enhance chrysin permeability through the Caco-2 monolayers. In conclusion, cyclodextrins, especially RAMEB, are suitable for improving chrysin solubility and absorption.

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Inhibitory Effects on NO Production and DPPH Radicals and NBT Superoxide Activities of Diarylheptanoid Isolated from Enzymatically Hydrolyzed Ehthanolic Extract of Alnus sibirica

Molecules ◽

10.3390/molecules24101938 ◽

2019 ◽

Vol 24 (10) ◽

pp. 1938 ◽

Cited By ~ 2

Author(s):

Hye Soo Wang ◽

Yoon Jeong Hwang ◽

Jun Yin ◽

Min Won Lee

Keyword(s):

Enzymatic Hydrolysis ◽

Biological Activities ◽

Biological Effects ◽

No Production ◽

Anti Cancer ◽

Geographically Distributed ◽

Anti Oxidant ◽

Anti Inflammatory ◽

Hydrolysis Of

Alnus sibirica (AS) is geographically distributed in Korea, Japan, Northeast China, and Russia. Various anti-oxidant, anti-inflammation, anti-atopic dermatitis and anti-cancer biological effects of AS have been reported. Enzymatic hydrolysis decomposes the sugar bond attached to glycoside into aglycone which, generally, has a superior biological activity, compared to glycoside. Enzymatic hydrolysis of the extract (EAS) from AS was processed and the isolated compounds were investigated—hirsutanonol (1), hirsutenone (2), rubranol (3), and muricarpon B (4). The structures of these compounds were elucidated, and the biological activities were assessed. The ability of EAS and the compounds (1–4) to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and Nitroblue tetrazolium (NBT) superoxide, and to inhibit NO production was evaluated in vitro. EAS showed more potent antioxidant and anti-inflammatory activity than AS. All investigated compounds showed excellent antioxidant and anti-inflammatory activities.

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