Inhibitory Effects on NO Production and DPPH Radicals and NBT Superoxide Activities of Diarylheptanoid Isolated from Enzymatically Hydrolyzed Ehthanolic Extract of Alnus sibirica

Alnus sibirica (AS) is geographically distributed in Korea, Japan, Northeast China, and Russia. Various anti-oxidant, anti-inflammation, anti-atopic dermatitis and anti-cancer biological effects of AS have been reported. Enzymatic hydrolysis decomposes the sugar bond attached to glycoside into aglycone which, generally, has a superior biological activity, compared to glycoside. Enzymatic hydrolysis of the extract (EAS) from AS was processed and the isolated compounds were investigated—hirsutanonol (1), hirsutenone (2), rubranol (3), and muricarpon B (4). The structures of these compounds were elucidated, and the biological activities were assessed. The ability of EAS and the compounds (1–4) to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and Nitroblue tetrazolium (NBT) superoxide, and to inhibit NO production was evaluated in vitro. EAS showed more potent antioxidant and anti-inflammatory activity than AS. All investigated compounds showed excellent antioxidant and anti-inflammatory activities.

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Evidences of Herbal Medicine-Derived Natural Products Effects in Inflammatory Lung Diseases

Mediators of Inflammation ◽

10.1155/2016/2348968 ◽

2016 ◽

Vol 2016 ◽

pp. 1-14 ◽

Cited By ~ 18

Author(s):

Fernanda Paula R. Santana ◽

Nathalia M. Pinheiro ◽

Márcia Isabel B. Mernak ◽

Renato F. Righetti ◽

Mílton A. Martins ◽

...

Keyword(s):

Natural Products ◽

Herbal Medicine ◽

Biological Activities ◽

Biological Effects ◽

Pulmonary Inflammation ◽

Experimental Models ◽

Chronic Obstructive ◽

Anti Inflammatory

Pulmonary inflammation is a hallmark of many respiratory diseases such as asthma, chronic obstructive pulmonary disease (COPD), and acute respiratory syndrome distress (ARDS). Most of these diseases are treated with anti-inflammatory therapy in order to prevent or to reduce the pulmonary inflammation. Herbal medicine-derived natural products have been used in folk medicine and scientific studies to evaluate the value of these compounds have grown in recent years. Many substances derived from plants have the biological effectsin vitroandin vivo, such as flavonoids, alkaloids, and terpenoids. Among the biological activities of natural products derived from plants can be pointed out the anti-inflammatory, antiviral, antiplatelet, antitumor anti-allergic activities, and antioxidant. Although many reports have evaluated the effects of these compounds in experimental models, studies evaluating clinical trials are scarce in the literature. This review aims to emphasize the effects of these different natural products in pulmonary diseases in experimental models and in humans and pointing out some possible mechanisms of action.

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Insightful Valorization of the Biological Activities of Pani Heloch Leaves through Experimental and Computer-Aided Mechanisms

Molecules ◽

10.3390/molecules25215153 ◽

2020 ◽

Vol 25 (21) ◽

pp. 5153

Author(s):

Naureen Banu ◽

Najmul Alam ◽

Mohammad Nazmul Islam ◽

Sanjida Islam ◽

Shahenur Alam Sakib ◽

...

Keyword(s):

Secondary Metabolites ◽

Methanol Extract ◽

Biological Activities ◽

Biological Effects ◽

Experimental Models ◽

Clot Lysis ◽

Control Group ◽

Anti Inflammatory

Pani heloch (Antidesma montanum) is traditionally used to treat innumerable diseases and is a source of wild vegetables for the management of different pathological conditions. The present study explored the qualitative phytochemicals; quantitative phenol and flavonoid contents; in vitro antioxidant, anti-inflammatory, and thrombolytic effects; and in vivo antipyretic and analgesic properties of the methanol extract of A. montanum leaves in different experimental models. The extract exhibited secondary metabolites including alkaloids, flavonoids, flavanols, phytosterols, cholesterols, phenols, terpenoids, glycosides, fixed oils, emodines, coumarins, resins, and tannins. Besides, Pani heloch showed strong antioxidant activity (IC50 = 99.00 µg/mL), while a moderate percentage of clot lysis (31.56%) in human blood and significant anti-inflammatory activity (p < 0.001) was achieved with the standard. Moreover, the analgesic and antipyretic properties appeared to trigger a significant response (p < 0.001) relative to in the control group. Besides, an in silico study of carpusin revealed favorable protein-binding affinities. Furthermore, the absorption, distribution, metabolism, excretion, and toxicity analysis and toxicological properties of all isolated compounds adopted Lipinski’s rule of five for drug-like potential and level of toxicity. Our research unveiled that the methanol extract of A. montanum leaves exhibited secondary metabolites that are a good source for managing inflammation, pyrexia, pain, and cellular toxicity. Computational approaches and further studies are required to identify the possible mechanism which responsible for the biological effects.

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SYNTHESIS AND CHARACTERIZATION FOR VARIOUS BIOLOGICAL ACTIVITIES OF TRANSITION METAL COMPLEXES OF QUERCETIN

INDIAN DRUGS ◽

10.53879/id.49.09.p0015 ◽

2012 ◽

Vol 49 (09) ◽

pp. 15-22

Author(s):

M. K Gadhwal ◽

◽

S. C Patil ◽

P Dmello ◽

U Joshi .

Keyword(s):

Transition Metal ◽

Metal Complexes ◽

Transition Metal Complexes ◽

Biological Activities ◽

Hydroxyl Groups ◽

Structural Modifications ◽

Cu Complex ◽

Anti Cancer ◽

Anti Oxidant ◽

Anti Inflammatory

Flavonoids are a group of polyphenolic compounds with multiple biological activities including antioxidant, anti-inflammatory and anti-cancer activities. The reduced bioavailability of flavonoids, however calls for structural modifications. Quercetin possesses multiple hydroxyl groups and an ability to chelate metal ions. Transition metal complexes have shown therapeutic promises in treatment of both inflammation and cancer. The present work describes synthesis of transition metal complexes of quercetin which resulted in the synthesis of the complexes of the type M2L which were characterized by IR and proton NMR. Although anti-oxidant and anti-inflammatory activity of the quercetin-Cu complex was higher than quercetin, the anticancer activity of the quercetin-Zn complex was much higher. A mechanism of action for the same is proposed.

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FORMULATION, CHARACTERIZATION AND EVALUATION OF ANTI-INFLAMMATORY AND ANTI-ANGIOGENIC ACTIVITIES OF MEMECYLAENE NANOEMULSION

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v8i5-s.1915 ◽

2018 ◽

Vol 8 (5-s) ◽

pp. 126-131

Author(s):

ND Rekha ◽

Dattatri K. Nagesha ◽

PH Rajasree ◽

N Shruthi

Keyword(s):

Zeta Potential ◽

Biological Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

In Vivo Model ◽

Particle Size Range ◽

Nitric Oxide Radical ◽

Anti Oxidant ◽

Anti Inflammatory

The present study was undertaken to formulate and evaluate the anti-inflammatory, anti-oxidant and anti-angiogenic activities of nanoemulsion of Memecylaene. Memecylaene was isolated from the leaves of Memecylon malabaricum by using various chromatographic methods. An oil-in-water (O/W) nanoemulsion of Memecylaene was formulated by sonication method using sunflower oil (oil phase), Tween 80 (Surfactant) and Ethanol (co-surfactant). The prepared nanoemulsion was characterized for its droplet size, poly dispersity index and zeta potential. Stability studies were performed and the nanoemulsions were subjected to different biological activities. The formulated nanoemulsion had a particle size range of 52.02 nm to 59.47 nm and zeta potential of -1.27 mV. The enhanced activity of Memecylaene, encapsulated in O/W emulsions is evidenced by the inhibition of phospholipase (PLA2) enzyme and H+, K+ -ATPase and thus showing anti-inflammatory and anti-secretagogues effects. The in vitro anti-oxidant activity was evaluated by DPPH radical and Nitric oxide radical scavenging activity. Further, the inhibition of the growth of neo vessels formation in the in-vivo model system of chick chorioallantoic membrane (CAM) assay, which is angiogenesis dependent, was also observed. The above findings would help in understanding the putative potential of Memecylaene-loaded nanoemulsion as a therapeutic agent. Keywords: Anti-angiogenesis, Anti-oxidant, Gastric (H+ K+), Memecylaene, Nanoemulsion, Phospholipase A2 (PLA2).

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Phytochemical Evaluation and Anti-Inflammatory Activity of Ethanolic Extract of Calotropis procera Leaves

RADS Journal of Biological Research & Applied Sciences ◽

10.37962/jbas.v11i1.243 ◽

2020 ◽

Vol 11 (1) ◽

pp. 1-6

Author(s):

Naveed Aslam Dogar ◽

Hamza Shahid ◽

Hafiz Usama Shaukat ◽

M. Abubakar Khan ◽

Farooq Saleem

Keyword(s):

Inflammatory Diseases ◽

Biological Activities ◽

Diclofenac Sodium ◽

Ethanolic Extract ◽

Inflammatory Activity ◽

Calotropis Procera ◽

Anti Cancer ◽

Ethanolic Extraction ◽

Anti Inflammatory

Background: Medicinal plants have been used since centuries to cure various diseases. There is a huge potential to investigate the medicinal impacts of different parts of plants. Roots, stem, leaves and fruits of Calotropis procera are known for their biological activities. Calotropis procera plant shows multiple pharmacological activities like anti-cancer, anti-microbial, antioxidant, anti-malarial, hepatoprotective and anti-diabetic activities. Objectives: The objective of the current research was ethanolic extraction of Calotropis procera leaves and to study the phytochemistry and anti-inflammatory activity. Methodology: In this effort, we used the extract of Calotropis procera leaves for detection of phytochemicals and anti-inflammatory activity in vitro by hypotonicity induced hemolysis on 2% HRBC suspension, using UV-Vis spectrophotometer. Results: Phytochemicals like alkaloids, terpenoids, and flavonoids were present in large amount while tannins, saponins, steroids and cardiac glycosides were in small amount, whereas phlobatannins and anthraquinone were not detected. The potential of the ethanolic extract of Calotropis procera leaves was compared with Diclofenac sodium (100μl/ml, 200μl/ml). The leaves extract of Calotropis procera (100, 200, 300, 400, 500μl/ml each) showed significant anti-inflammatory activity by hypotonicity induced hemolysis on 2% HRBC suspension. Conclusion: The Calotropis procera leaves have potential to cure inflammatory diseases and can be used as anti-inflammatory medicine and analgesic.

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Effects of Namya Kanom Jeen powder extracts on anti-oxidative and anti-inflammatory properties

Functional Foods in Health and Disease ◽

10.31989/ffhd.v8i8.383 ◽

2018 ◽

Vol 8 (8) ◽

pp. 379 ◽

Cited By ~ 1

Author(s):

Preeya Dat-arun ◽

Rattana Leelawattana ◽

Pavinee Chinachoti

Keyword(s):

Functional Properties ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Water Extract ◽

No Production ◽

Scavenging Activity ◽

Anti Oxidant ◽

Anti Inflammatory ◽

Anti Inflammation

Background: Spices and herbs are known to have anti-oxidant and anti-inflammatory properties. We studied their properties of Namya Kanom Jeen (NKJ), a spicy soup (Southern Thailand recipe) with water and ethanol (50% and 95%) extracts.Methods: This study aimed to assess functional properties of NKJ powder extract using in vitro model. These functional properties were anti-oxidant, α-amylase inhibition, and anti-inflammatory properties. Anti-oxidant activities were determined using free radical scavenging activity (DPPH) and Ferric Reducing Anti-oxidant Power (FRAP). Anti-inflammation effect was studied by measuring nitric oxide (NO) production inhibition on RAW264.7 macrophage cells after being exposed to lipopolysaccharide (LPS). Results: Water extract of NKJ powder demonstrated the highest activity in anti-inflammatory and anti-oxidant property by DPPH radical scavenging activity when compared with ethanol extract. Conclusion: NKJ powder extracts may ameliorate the oxidation and inflammation conditions which would be the combined effect of several bioactive compounds from many of the ingredients within these extracts.Keywords: Anti-oxidant; Anti-inflammation; Phenolic compound; Namya Kanom Jeen powder

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Evaluation of in vitro antioxidant, anti-inflammatory, anticoagulant and antiplatelet potential of Rhus mysorensis

Biomedicine ◽

10.51248/.v41i4.1365 ◽

2021 ◽

Vol 41 (4) ◽

pp. 724-731

Author(s):

Ghouseul Azam ◽

Sathisha G. Jayanna ◽

Anitha Nelliankla ◽

Vasanthraj Boraiah ◽

Sujatha M. Hanumegowda ◽

...

Keyword(s):

Biological Activities ◽

Anticoagulant Activity ◽

Stem Bark ◽

No Production ◽

Human Beings ◽

Column Fraction ◽

Anti Inflammatory ◽

Plasma Recalcification Time ◽

Recalcification Time

Since ancient times human beings are using plant-based medicines for the treatment of various ailments, especially in the rural areas, due to their availability and affordability. Rhus mysorensis (RM) is widely used as a traditional medicine to treat various ailments. Owing to its potential medicinal value, the present study was designed to explore the in vitro antioxidant, anti-inflammatory, anticoagulant and antiplatelet properties of purified column fraction of RM. The methanol extract of stem bark powder was sequentially fractioned by solvent partitioning. The liquid methanol fraction was further fractionated by column chromatography using gradient elution. Eluted fractions were analyzed using HPLC for percentage purity and yield. The fraction with higher percentage of purity and yield was assessed for in vitro antioxidant activity by measuring SOD and GPx activities, anti-inflammatory activity by the inhibition of nitric oxide (NO) production in LPS induced RAW264.7 cells, anticoagulation by plasma recalcification time and antiplatelet activity by agonists induced platelet aggregation respectively. The antioxidant potency of column fraction (B8) revealed that, highest enzyme activities were recorded at a concentration of 320µg/ml. The enzyme activity was found to be 2.45 U/ml for SOD and 135.75 U/L for GPx respectively. Purified column fraction (B8) of RM significantly reduces the production of NO in LPS stimulated RAW 264.7 cell lines at 320????g/ml concentration with 31.90% of inhibition. The anticoagulant activity of purified fraction was determined in terms of plasma recalcification time. Interestingly, the fraction showed the most potent anticoagulant activity both in PRP and PPP as it prolonged the clotting time. The findings indicate that the stem bark of RM possesses potent antioxidant, anti-inflammatory, anticoagulant and antiplatelet activities, supporting the use of this species for treating oxidative stress-induced inflammatory diseases. Further, bioactivity guided fractionation studies to characterize and identify specific phytochemicals responsible for these biological activities are needed.

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Anti-Inflammatory Activities of the Ethanol Extract of Prasiola japonica, an Edible Freshwater Green Algae, and Its Various Solvent Fractions in LPS-Induced Macrophages and Carrageenan-Induced Paw Edema via the AP-1 Pathway

Molecules ◽

10.3390/molecules27010194 ◽

2021 ◽

Vol 27 (1) ◽

pp. 194

Author(s):

Laily Rahmawati ◽

Sang Hee Park ◽

Dong Seon Kim ◽

Hwa Pyoung Lee ◽

Nur Aziz ◽

...

Keyword(s):

Molecular Mechanisms ◽

Biological Activities ◽

Ethanol Extract ◽

Raw264.7 Cells ◽

No Production ◽

Paw Edema ◽

Chain Reactions ◽

Anti Inflammatory

Prasiola japonica possesses several biological activities. However, reports on the anti-inflammatory activities and molecular mechanisms of its different solvent fractions remain limited. In this study, we investigated the potential anti-inflammatory activities of P. japonica ethanol extract (Pj-EE) and four solvent fractions of Pj-EE made with hexane (Pj-EE-HF), chloroform (Pj-EE-CF), butanol (Pj-EE-BF), or water (Pj-EE-WF) in both in vitro (LPS-induced macrophage-like RAW264.7 cells) and in vivo (carrageenan-induced acute paw edema mouse models) experiments. The most active solvent fraction was selected for further analysis. Various in vitro and in vivo assessments, including nitric oxide (NO), cytokines, luciferase assays, real-time polymerase chain reactions, and immunoblotting analyses were performed to evaluate the underlying mechanisms. In addition, the phytochemical constituents were characterized by Liquid chromatography-tandem mass spectrometry. In in vitro studies, the highest inhibition of NO production was observed in Pj-EE-CF. Further examination revealed that Pj-EE-CF decreased the expression of inflammation-related cytokines in LPS-induced RAW264.7 cells and suppressed subsequent AP-1-luciferase activity by inhibition of phosphorylation events in the AP-1 signaling pathway. Pj-EE-CF treatment also demonstrated the strongest reduction in thickness and volume of carrageenan-induced paw edema, while Pj-EE-BF showed the lowest activity. Furthermore, Pj-EE-CF also reduced gene expression and cytokines production in tissue lysates of carrageenan-induced paw edema. These findings support and validate the evidence that Pj-EE, and especially Pj-EE-CF, could be a good natural source for an anti-inflammatory agent that targets the AP1 pathway.

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Phytochemical screening & in-vitro evaluation of anti-inflammatory activity of clerodendrum indicum roots

World Journal of Current Medical and Pharmaceutical Research ◽

10.37022/wjcmpr.v3i6.201 ◽

2021 ◽

pp. 140-143

Author(s):

Sushma M ◽

Lahari S ◽

Mounika A ◽

Sailaja K.E

Keyword(s):

Ethanolic Extract ◽

Inflammatory Activity ◽

Phytochemical Screening ◽

In Vitro Evaluation ◽

Anti Cancer ◽

The Family ◽

Steroid Glycosides ◽

Anti Oxidant ◽

Anti Inflammatory

This study aims to extract the phytochemical constituents and in-vitro evaluation of anti-inflammatory activity of roots of clerodendrum Indicum. Clerodendrum indicum (L.) kuntz. Commonly known as bharangi or chingari, belongs to the family verbenaceae. The plant is expectorant, stomachic, Anti-bronchitis, Anti-nociceptive, Anti-microbial, Anti-diarrheal, anti-oxidant and anti-cancer. Preliminary phytochemical screening revealed the presence of flavonoids, steroids, steroid glycosides, terpenoids etc. This study has taken with the Anti-inflammatory activity and preliminary phytochemical screening on the clerodendrum indicum. In the present study, qualitative study of the phytochemicals present in the sample was determined, Anti-inflammatory activity were carried out. On the premise of the prevailing study, the outcomes eventually concluded that the ethanolic extract of clerodendrum indicum own anti inflammatory pastime. This evaluation offers an concept that the compound of the plant clerodendrum indicum may be used as lead compound for designing robust pills which may be used for remedy of diverse diseases.

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Chemical Composition, Antioxidant and Anti-Inflammatory Activities of Clary Sage and Coriander Essential Oils Produced on Polluted and Amended Soils-Phytomanagement Approach

Molecules ◽

10.3390/molecules26175321 ◽

2021 ◽

Vol 26 (17) ◽

pp. 5321

Author(s):

Robin Raveau ◽

Joël Fontaine ◽

Anthony Verdin ◽

Loris Mistrulli ◽

Frédéric Laruelle ◽

...

Keyword(s):

Essential Oils ◽

Biological Activities ◽

Biological Effects ◽

Biological Properties ◽

Chemical Compositions ◽

Experimental Conditions ◽

Aerial Parts ◽

Mycorrhizal Inoculation ◽

Anti Inflammatory

The potential of essential oils (EO), distilled from two aromatic plants—clary sage (Salvia sclarea L.) and coriander (Coriandrum sativum L.)—in view of applications as natural therapeutic agents was evaluated in vitro. These two were cultivated on a trace element (TE)-polluted soil, as part of a phytomanagement approach, with the addition of a mycorrhizal inoculant, evaluated for its contribution regarding plant establishment, growth, and biomass production. The evaluation of EO as an antioxidant and anti-inflammatory, with considerations regarding the potential influence of the TE-pollution and of the mycorrhizal inoculation on the EO chemical compositions, were the key focuses. Besides, to overcome EO bioavailability and target accession issues, the encapsulation of EO in β-cyclodextrin (β-CD) was also assessed. Firstly, clary sage EO was characterized by high proportions of linalyl acetate (51–63%) and linalool (10–17%), coriander seeds EO by a high proportion of linalool (75–83%) and lesser relative amounts of γ-terpinene (6–9%) and α-pinene (3–5%) and coriander aerial parts EO by 2-decenal (38–51%) and linalool (22–39%). EO chemical compositions were unaffected by both soil pollution and mycorrhizal inoculation. Of the three tested EO, the one from aerial parts of coriander displayed the most significant biological effects, especially regarding anti-inflammatory potential. Furthermore, all tested EO exerted promising antioxidant effects (IC50 values ranging from 9 to 38 g L−1). However, EO encapsulation in β-CD did not show a significant improvement of EO biological properties in these experimental conditions. These findings suggest that marginal lands polluted by TE could be used for the production of EO displaying faithful chemical compositions and valuable biological activities, with a non-food perspective.

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