COLLECT EXTRACELLULAR MATRIX FROM IN VITRO FIBROBLAST
2009 ◽
Vol 12
(9)
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pp. 5-11
Keyword(s):
Triton X
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Extracellular matrices (ECM) have been reported to enhance cell attachment and proliferation as well as to create stem cell niches invitro. We harvested ECM from human fibroblasts for a number of researches, including tissue engineering. Fibroblasts were isolated from human foreskins, cultured in DMEMF12 containing 10% FBS and identified by Trichrome staining and immunohistochemistry for Vimentin. Then, fibroblasts were stimulated to synthetize ECM in medium supplemented 0.05% ascorbic acid. Cell constituents were removed by using Triton X-100, NH4OH and DNAse. ECM proteins were evaluated by PAS staining. Results showed that collagen is present in ECM.
2011 ◽
Vol 81
(1)
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pp. 34-42
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Keyword(s):
2020 ◽
Vol 51
(4)
◽
pp. 1038-1047
2010 ◽
Vol 15
(1)
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pp. 19-23
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Keyword(s):