scholarly journals Establishing pinostrobin reference standard for quantitative analysis of the rhizomes of Boesenbergia pandurata

2020 ◽  
Vol 4 (4) ◽  
pp. first
Author(s):  
Nhan Trung Nguyen ◽  
Truong Nhat Van Do ◽  
An Phu Thi Do ◽  
Mai Thanh Thi Nguyen
Keyword(s):  
Food Processing ◽  
Quality Evaluation ◽  
Biological Activities ◽  
Hplc Method ◽  
Viet Nam ◽  
Reference Standard ◽  
Main Ingredient ◽  
System Suitability ◽  
Quantitative Hplc Analysis ◽  
Quantitative Hplc

Boesenbergia pandurata Roxb. Schlecht. (Zingiberaceae), called ``Ngai bun'' in Viet Nam, is one of the Southeast Asian medicinal plants and its rhizomes are used primarily as a spice. This is a perennial, short-stemmed plant, formed by leaf sheaths and can grow up to 50 cm. The leaves are 7-11 cm wide and 25-50 cm long. Its rhizome surfaces are light brown in color, the inner rhizome is yellow, oval-shaped, and has a very aromatic odor. In folklore, Boesenbergia pandurata rhizomes are used as a spice for food processing. This plant contains pinostrobin as the major constituents. Previously showed that pinostrobin compound is the main ingredient together with a variety of biological activities such as antibacterial, inhibition of free radicals, ... Pinostrobin is necessary composition for the screening, testing, and quality evaluation of the rhizomes of B. pandurata species and others in the Zingiberaceae family. This research had conducted a reference standard of pinostrobin isolated from the rhizomes of B. pandurata had 99.26 % purity, which is reliable in medicinal testing. An HPLC method for pinostrobin determination was conducted and The quantitative HPLC analysis was validated for system suitability, selectivity, linearity ranges, and precision. Application of the process to investigate the preparation of extract shown that reflux extraction with ethanol obtained the highest pinostrobin content with 22.05 % in extracts and 2.89 % in dried rhizomes of B. pandurata.

scholarly journals CHEMOMETRICS ASSISTED VALIDATED HPLC METHOD DETECTING SEASONAL VARIANCES OF SECONDARY METABOLITES OF Camellia sinensis CONSIDERING THE ABIOTIC STRESSES

2019 ◽  
Vol 4 (2) ◽  
pp. 9
Author(s):  
Koushik Bhandari ◽  
Tridib Kumar Goswami ◽  
Baishakhi De
Keyword(s):  
Abiotic Stress ◽  
Secondary Metabolites ◽  
Black Tea ◽  
Hplc Method ◽  
Stress Factors ◽  
Tea Leaves ◽  
Abiotic Stress Factors ◽  
Quantitative Hplc Analysis ◽  
Experimental Findings ◽  
Quantitative Hplc

Climatic changes have great impact on the crops and agro-eco systems and such changes influences the concentration of secondary metabolites. IIT Kharagpur, India is a non-traditional tea growing zone where Tocklai Vegetative 25 variety was used as the research material. This study reports the development of a chemometrics assisted HPLC method validated as per ICH guidelines to explore the effect of seasonal variations in polyphenolics viz. catechins and methyl xanthenes like caffeine in fresh tea leaves and processed CTC black tea prepared from them. Further study was done on the variances amongst the concentration of secondary metabolites and abiotic stress factors. Good resolutions of secondary metabolites were obtained using 92% of 0.2% acetic acid and 8% of acetonitrile as the mobile phase, with a flow rate of 1 mL/ min, injection volume of 20 µl,  PDA detector was set at 200-600 nm and chromatograms were recorded at 274 nm. Results of quantitative HPLC analysis have clearly shown that highest yield of catechins and caffeine were observed in fresh tea leaves plucked during spring (24.3ºC temperature and average rainfall of 34 mm) and also the processed black tea made from it, followed by tea leaves plucked during monsoon (28.8 ºC temperature and 282 mm rainfall) and processed black tea prepared from it. The lowest concentrations of secondary metabolites were found in leaves plucked during autumn (26.2 °C temperature and 132 mm rainfall) and the processed tea prepared from it. The developed quantitative HPLC method showed an inter day precision of 0.3, intraday precision of 0.2, repeatability value of 0.31, ruggedness value of 0.33 and robustness value of 0.2. Considering temperature and rainfall as abiotic stress factors, highest total polyphenolic content was obtained during spring and lowest in autumn. From our experimental findings, the fresh tea leaves of spring season and also the processed black tea prepared from it showed higher yield of catechins.

scholarly journals Analysis of Homoisoflavonoids in Caesalpinia digyna by HPLC-ESI-MS, HPLC and Method Validation

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Somendu K. Roy ◽  
Amit Srivastava ◽  
Sanjay M. Jachak
Keyword(s):  
Method Validation ◽  
Mobile Phase ◽  
Hplc Method ◽  
Good Recovery ◽  
Esi Ms ◽  
Validation Parameters ◽  
Quantitative Hplc Analysis ◽  
Quantitative Hplc ◽  
Hplc Analyses

The roots of Caesalpinia digyna have been reported to contain gallic acid derivatives and minor homoisoflavonoids, but HPLC-ESI-MS and HPLC analyses of the homoisoflavonoids were challenging due to their low concentration in the roots. Separation and identification was accomplished by HPLC-ESI-MS and further elaborated for quantification using a C18 column with detection at 330 nm. A gradient mobile phase consisting of methanol and water (0.1% acetic acid) was used. The developed HPLC method showed good linearity (r2≥0.998), high precision (RSD<5%) and a good recovery (99.3-104.5%) of the compounds. The lowest detection limit was 0.75 ng and the method was found to be robust. All the validation parameters were found to be within the permissible limits and, therefore, the developed method is accurate and reliable for the quality control of C. digyna and other Caesalpinia species. This is the first report of sample preparation on Diaion HP-20 resin and characterization of homoisoflavonoids by HPLC-ESI-MS, extended by extensive quantitative HPLC analysis of homoisoflavonoids in C. digyna roots and method validation.

scholarly journals Evaluation of precipitation and direct methods for HDL-cholesterol assay by HPLC

1997 ◽  
Vol 43 (10) ◽  
pp. 1885-1890 ◽  
Author(s):  
Mitsuyo Okazaki ◽  
Keiko Sasamoto ◽  
Toshio Muramatsu ◽  
Seijin Hosaki
Keyword(s):  
Direct Method ◽  
Direct Methods ◽  
Hdl Cholesterol ◽  
Hplc Method ◽  
Alternative Methods ◽  
Cholesterol Concentration ◽  
Precipitation Method ◽  
Qualitative Information ◽  
Quantitative Hplc Analysis ◽  
Quantitative Hplc

Abstract HDL-cholesterol (HDL-C) values measured by precipitation (sodium phosphotungstate–MgCl2) and direct methods were compared with those obtained by HPLC with a new column (TSKgel Lipopropak) and an eluent (TSKeluent LP-1). The HDL-C values determined by the precipitation method were significantly (P &lt;0.001) lower than those by the HPLC method, whereas the HDL-C values by the direct method were slightly but significantly higher (P &lt;0.02) than those by the HPLC method. A quantitative HPLC analysis of the cholesterol concentration in HDL and non-HDL fractions in the supernatant of serum separated by precipitation reagents with different MgCl2 concentrations ranging from 7.3 to 44 mmol/L revealed that reagents with &gt;22 mmol/L MgCl2 precipitated part of HDL as well as non-HDL lipoproteins. The HPLC method providing quantitative and qualitative information with high precision was regarded as being a reliable approach for HDL-C assay. The HPLC can be also used to evaluate alternative methods for cholesterol assay.

scholarly journals Quantification and Comparison of Extraction Methods for Alkaloids in Aegle marmelos Leaves by HPLC

2014 ◽  
Vol 9 (7) ◽  
pp. 1934578X1400900
Author(s):  
Aniket Karmase ◽  
K Prasanna ◽  
Sruti Rasabattula ◽  
Kamlesh K Bhutani
Keyword(s):  
Hplc Analysis ◽  
Extraction Methods ◽  
Hplc Method ◽  
Good Recovery ◽  
Aegle Marmelos ◽  
Low Concentrations ◽  
Validation Parameters ◽  
Marker Compounds ◽  
Quantitative Hplc Analysis ◽  
Quantitative Hplc

The leaves of Aegle marmelos are reported to contain multi-bioactive classes of compounds including coumarins, furanocoumarins and alkaloids. HPLC analysis of the crude extract was challenging due to low concentrations of the compounds in the leaves. Five compounds visible in the HPLC chromatogram were separated and identified by HPLC and further elaborated for quantification as marker compounds of A. marmelos leaves using a C18 column with detection at 275 nm. A gradient mobile phase consisting of acetonitrile and water was used. The developed HPLC method showed good linearity (r2≥0.994), high precision (RSD<5%), and good recovery (99.27–99.98%) of the compounds. The lowest detection limit was 5 ng and the method was found to be robust. All the validation parameters were within the permissible limits. Therefore, the developed method is accurate and reliable for the quality control of A. marmelos. This is the first report of extensive quantitative HPLC analysis of marker compounds in A. marmelos leaves and method validation.

scholarly journals Employing an R Software Package rsm for Optimizing of Genistein, Daidzein, and Glycitein Separation and Its Application for Soy Milk Analysis by HPLC Method

2020 ◽  
Vol 20 (5) ◽  
pp. 1184
Author(s):  
Florentinus Dika Octa Riswanto ◽  
Alni Desra ◽  
Rinjani Mustika Sari ◽  
Valentino Thomas ◽  
Abdul Rohman ◽  
...  
Keyword(s):  
High Performance ◽  
Quantitative Estimation ◽  
Biological Activities ◽  
Reversed Phase ◽  
Hplc Method ◽  
Milk Analysis ◽  
Soy Milk ◽  
Hplc Separation ◽  
Column Temperature ◽  
System Suitability

Soy milk, one of the soybean products, become more popular in recent years due to its benefit for human health. Biological activities of soybean products have been widely studied according to the presence of isoflavone aglycones content, including genistein, daidzein, and glycitein. Hence, it is important to develop an effective and efficient analytical method to provide guidance regarding appropriate isoflavone intake levels for soy milk. A reversed-phase high performance liquid chromatography (HPLC) method was developed and optimized in this study employed by R statistical software with the package of rsm. A C18 column was used for HPLC separation with the detection at 260 nm. Optimized condition for HPLC separation has been achieved with the mobile phase of methanol: water (63.26:36.74), a flow rate of 0.81 mL/min, and a column temperature of 45.31 °C. These conditions were applied in the HPLC system and successfully tested for the system suitability. Quantitative estimation was performed and resulted that the genistein, daidzein, and glycitein content in soy milk samples were 6.372, 6.273, and 2.853 µg/mL, respectively.

Quantitative HPLC analysis of chamuangone in Garcinia cowa leaf extracts

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
P Panichayupakaranant ◽  
A Sakunpak
Keyword(s):  
Hplc Analysis ◽  
Leaf Extracts ◽  
Garcinia Cowa ◽  
Quantitative Hplc Analysis ◽  
Quantitative Hplc

scholarly journals Box-Behnken Assisted Validation and Optimization of an RP-HPLC Method for Simultaneous Determination of Domperidone and Lansoprazole

Separations ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 5
Author(s):  
Mohd Afzal ◽  
Mohd. Muddassir ◽  
Abdullah Alarifi ◽  
Mohammed Tahir Ansari
Keyword(s):  
Flow Rate ◽  
Mobile Phase ◽  
Limit Of Detection ◽  
Hplc Method ◽  
Box Behnken Design ◽  
Rp Hplc ◽  
System Suitability ◽  
Method Optimization ◽  
Key Variables

A highly specific, accurate, and simple RP-HPLC technique was developed for the real-time quantification of domperidone (DOMP) and lansoprazole (LANS) in commercial formulations. Chromatographic studies were performed using a Luna C8(2), 5 μm, 100Å, column (250 × 4.6 mm, Phenomenex) with a mobile phase composed of acetonitrile/2 mM ammonium acetate (51:49 v/v), pH 6.7. The flow rate was 1 mL·min−1 with UV detection at 289 nm. Linearity was observed within the range of 4–36 µg·mL−1 for domperidone and 2–18 µg·mL−1 for lansoprazole. Method optimization was achieved using Box-Behnken design software, in which three key variables were examined, namely, the flow rate (A), the composition of the mobile phase (B), and the pH (C). The retention time (Y1 and Y3) and the peak area (Y2 and Y4) were taken as the response parameters. We observed that slight alterations in the mobile phase and the flow rate influenced the outcome, whereas the pH exerted no effect. Method validation featured various ICH parameters including linearity, limit of detection (LOD), accuracy, precision, ruggedness, robustness, stability, and system suitability. This method is potentially useful for the analysis of commercial formulations and laboratory preparations.

scholarly journals Phtytochemical Studies and Quantitative HPLC Analysis of Rosmarinic Acid and Luteolin 5-O-β-D-Glucopyranoside on Thymus praecox subsp. grossheimii var. grossheimii

2015 ◽  
Vol 63 (9) ◽  
pp. 720-725 ◽  
Author(s):  
Handan Gokben Sevindik ◽  
Ufuk Ozgen ◽  
Alptug Atila ◽  
Handan Ozturk Er ◽  
Cavit Kazaz ◽  
...  
Keyword(s):  
Rosmarinic Acid ◽  
Hplc Analysis ◽  
Quantitative Hplc Analysis ◽  
Quantitative Hplc
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