The Pattern of Neuroinflammatory miRNAs, C-Reactive Protein, and Alanine Aminotransferase During Hospitalization In Recovered or Not-Recovered COVID-19 Patients

Background: Our aim was to investigate the expression of miRNAs, C-reactive protein as a blood inflammation marker, and alanine aminotransferase as a tissue inflammation marker in recovered and not-recovered COVID-19 patients. Methods: This cross-sectional project was done in three medical center of Iran from December to March, 2021. Generally, a total of 20 confirmed cases of COVID-19 with grade III and 20 healthy subjects were enrolled in the study. Then, the neuroinflammatory expression of miRNAs (miR-199, miR-203, and miR-181), C-reactive protein, and alanine aminotransferase were investigated during hospitalization from week 0 to week 2. Results: In not-recovered COVID-19 subjects, the expression of miR-199, miR-203, and miR-181 were decreased and the levels of C-reactive protein and alanine aminotransferase were increased during hospitalization. Conversely, in recovered COVID-19 subjects, the relative expression of miR-199, miR-203, and miR-181 were increased and the levels of C-reactive protein and alanine aminotransferase were decreased during hospitalization. Conclusions: The expression pattern of neuroinflammatory miRNAs is depends on whether the COVID-19 patient is recovering or deteriorating. Their expression is down-regulated in not-recovered COVID-19 patients and up-regulated in recovered COVID-19 patients.

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Perbedaan Kadar C-Reactive Protein pada Demam Akut karena Infeksi Dengue dan Demam Tifoid

Jurnal Penyakit Dalam Indonesia ◽

10.7454/jpdi.v3i3.24 ◽

2017 ◽

Vol 3 (3) ◽

pp. 138

Author(s):

Adeputri Tanesha Idhayu ◽

Lie Khie Chen ◽

Suhendro Suhendro ◽

Murdani Abdullah

Keyword(s):

Typhoid Fever ◽

Medical Center ◽

Demographic Data ◽

Dengue Infection ◽

Hemorrhagic Fever ◽

Cross Sectional Study ◽

C Reactive Protein ◽

Cross Sectional ◽

Protein Levels ◽

Reactive Protein

Pendahuluan. Infeksi dengue dan demam tifoid merupakan penyakit endemik di Indonesia. Namun pada awal awitan demam terdapat kesulitan dalam membedakan keduanya. Oleh karena itu dibutuhkan modalitas pemeriksaan penunjang yang sederhana untuk membantu diagnosis infeksi dengue dan demam tifoid. C-Reactive Protein (CRP) merupakan alat bantu diagnostik yang terjangkau, cepat dan murah untuk diagnosis penyebab demam akut. Penelitian ini bertujuan mengetahui perbedaan kadar CRP pada demam akut karena infeksi dengue dengan demam tifoid.Metode. Penelitian ini merupakan studi potong lintang pada pasien demam akut dengan diagnosis demam dengue/ demam berdarah dengue atau demam tifoid yang dirawat di IGD atau ruang rawat RSCM, RS Pluit dan RS Metropolitan Medical Center Jakarta dalam kurun waktu Januari 2010 sampai dengan Desember 2013. Kadar CRP yg diteliti adalah CRP yang diperiksa 2-5 hari setelah awitan demam. Data penyerta yang dikumpulkan adalah data demografis, data klinis, pemberian antibiotik selama perawatan, leukosit, trombosit, neutrofil, LED dan lama perawatan.Hasil. Sebanyak 188 subjek diikutsertakan pada penelitian ini, terdiri dari 102 pasien dengue dan 86 pasien demam tifoid. Median (RIK) CRP pada infeksi dengue 11,65 (16) mg/L dan pada demam tifoid 53 (75) mg/L. Terdapat perbedaan median CRP yang bermakna antara infeksi dengue dan demam tifoid (p <0,001). Pada titik potong persentil 99%, didapatkan hasil kadar CRP infeksi dengue sebesar 45,91 mg/L dan kadar CRP demam tifoid pada level persentil 1% sebesar 8 mg/L.Simpulan. Terdapat perbedaan kadar CRP pada demam akut karena infeksi dengue dengan demam tifoid. Pada titik potong persentil 99%, kadar CRP >45,91 mg/L merupakan diagnostik CRP untuk demam tifoid, kadar CRP <8 mg/L merupakan diagnostik CRP untuk infeksi dengue. kadar CRP 8-45,91 mg/L merupakan area abu-abu dalam membedakan diagnosis keduanya.Kata Kunci: dengue, demam tifoid, protein C-reaktif The Difference of C-Reactive Protein Levels in Acute Fever caused by Dengue and Typhoid InfectionsIntroduction. Dengue infection and typhoid fever are endemic disease in Indonesia. But in the early days of onset sometimes it is difficult to distinguish them. A simple modality test is needed to support the diagnosis. C-Reactive Protein (CRP) is an affordable, fast and relatively less expensive diagnostic tool to diagnose the causes of acute fever. This study was aimed to determine the differences of CRP level in the acute febrile caused by dengue infection or typhoid fever. Methods. A cross sectional study has been conducted among acute febrile patients with diagnosis of dengue fever/ dengue hemorrhagic fever or typhoid fever who admitted to the emergency room or hospitalized in Cipto Mangunkusumo Hospital, Pluit Hospital, and Metropolitan Medical Center Hospital Jakarta between January 2010 and December 2013. Data obtained from medical records. CRP used in this study was examined at 2-5 days after onset of fever. The other collected data were demographic data, clinical data, use of antibiotics, leukocytes, platelets, neutrophils, ESR, and length of stay in hospital. Results. 188 subjects met the inclusion criteria; 102 patients with dengue and 86 patients with typhoid fever. Median CRP levels in dengue infection was 11.65 (16) mg/L and in typhoid fever was 53 (75) mg/L. There were significant differences in median CRP levels between dengue infection and typhoid fever (p < 0.001). At the 99% percentile cut-off point, CRP levels for dengue infection was 45.91 mg/L and CRP levels for typhoid fever at 1% percentile was 8 mg / L. Conclusions. There was significantly different levels of CRP in acute fever due to dengue infection and typhoid fever. At the 99% percentile cut-off point, CRP level >45.91 mg/L was diagnostic for typhoid fever, CRP level <8 mg/L was diagnostic for dengue infection. CRP level between 8 to 45.91 mg/L was a gray area for determining diagnosis of dengue infection and typhoid fever. Keywords: C-reactive protein, dengue, typhoid fever

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Serum levels of YKL-40 and C reactive protein in patients with hip osteoarthritis and healthy subjects: a cross sectional study

Annals of the Rheumatic Diseases ◽

10.1136/ard.59.10.828 ◽

2000 ◽

Vol 59 (10) ◽

pp. 828-831 ◽

Cited By ~ 60

Author(s):

T. Conrozier

Keyword(s):

Healthy Subjects ◽

Hip Osteoarthritis ◽

Serum Levels ◽

Cross Sectional Study ◽

Sectional Study ◽

C Reactive Protein ◽

Cross Sectional ◽

Reactive Protein

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Serum high-sensitive C-reactive protein is associated with dietary intakes in diabetic patients with and without hypertension: a cross-sectional study

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1177/0004563217733286 ◽

2017 ◽

Vol 55 (4) ◽

pp. 422-429 ◽

Cited By ~ 2

Author(s):

Mohammad Bagherniya ◽

Sayyed Saeid Khayyatzadeh ◽

Ali reza Heidari Bakavoli ◽

Gordon A Ferns ◽

Mahmoud Ebrahimi ◽

...

Keyword(s):

Healthy Subjects ◽

Protein Concentration ◽

Cross Sectional Study ◽

Dietary Factors ◽

Diabetic Patients ◽

Sectional Study ◽

C Reactive Protein ◽

Cross Sectional ◽

Hypertensive Patients ◽

Reactive Protein

Background Serum high-sensitive C-reactive protein (hs-CRP) concentrations independently predict the development of diabetes, metabolic syndrome and cardiovascular disease. However, the impact of dietary factors on serum high-sensitive C-reactive protein concentrations in diabetic patients has received limited attention. We aimed to investigate the association between dietary factors and high-sensitive C-reactive protein , among diabetic patients with and without hypertension and healthy subjects. Methods In this cross-sectional study, diabetics with ( n = 325) and without hypertension ( n = 599) and healthy individuals ( n = 1220) were recruited in Mashhad, Iran. Dietary intake was assessed by 24-h recall. Biochemical parameters including serum high-sensitive C-reactive protein were measured using standard protocols. Stepwise multiple regression analysis was used to predict whether serum high-sensitive C-reactive protein concentration was associated with dietary constituents. Results High-sensitive C-reactive protein was significantly higher among hypertensive and non-hypertensive diabetic patients compared with healthy subjects ( P < 0.001). The dietary intake of zinc + 6.4% and calcium −3.4% and body mass index +3.9% explained approximately 13.7% of the variation in serum high-sensitive C-reactive protein among diabetic hypertensive patients. Approximately 9.7% of the variation in serum high-sensitive C-reactive protein in diabetic non-hypertensive patients could be explained by body mass index, and intake of sodium, iron and cholesterol. In the healthy subjects, approximately 4.4% of the total variation in serum high-sensitive C-reactive protein concentration could be explained by cholesterol consumption and waist circumference. Conclusion Serum high-sensitive C-reactive protein concentrations were found to be a significant predictor for hypertensive and non-hypertensive diabetic subjects. There was a significant association between dietary factors include zinc, iron, sodium and cholesterol and serum high-sensitive C-reactive protein, while there was an inverse association between dietary calcium and serum high-sensitive C-reactive protein in diabetic hypertensive individuals.

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Nocturnal intermittent hypoxia and C reactive protein among middle-aged community residents: a cross-sectional survey

Thorax ◽

10.1136/thx.2009.128744 ◽

2010 ◽

Vol 65 (6) ◽

pp. 523-527 ◽

Cited By ~ 16

Author(s):

I. Muraki ◽

T. Tanigawa ◽

K. Yamagishi ◽

S. Sakurai ◽

T. Ohira ◽

...

Keyword(s):

Intermittent Hypoxia ◽

C Reactive Protein ◽

Middle Aged ◽

Cross Sectional Survey ◽

Cross Sectional ◽

Reactive Protein ◽

Community Residents

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POS0765 LABORATORY RATIOS: A SUBROGATE BIOMARKER FOR DETECTION OF INFECTION IN SLE PATIENTS

Annals of the Rheumatic Diseases ◽

10.1136/annrheumdis-2021-eular.3184 ◽

2021 ◽

Vol 80 (Suppl 1) ◽

pp. 636.1-636

Author(s):

Y. Santamaria-Alza ◽

J. Sanchez-Bautista ◽

T. Urrego Callejas ◽

J. Moreno ◽

F. Jaimes ◽

...

Keyword(s):

Systemic Lupus Erythematosus ◽

Logistic Regression ◽

Roc Curve ◽

Lupus Erythematosus ◽

Statistical Significance ◽

C Reactive Protein ◽

Systemic Lupus ◽

Predictive Capacity ◽

Cross Sectional ◽

Reactive Protein

Background:The most common complication in patients with SLE is infection, and its clinical presentation is often indistinguishable from SLE flares. Therefore, laboratory ratios have been evaluated to differentiate between those events. Among them, ESR/CRP1, neutrophil/lymphocyte (NLR)2, and platelet/lymphocyte (PLR)3 ratios have been previously assessed with acceptable performance; however, there is no validation of those ratios in our SLE population.Objectives:To examine the predictive capacity of infection of the lymphocyte/C4 (LC4R), lymphocyte/C3 (LC3R), and ferritin/ESR (FER) ratios in SLE patients, and to evaluate the performance of ESR/CRP, NLR, AND PLR ratios in our SLE population.Methods:We conducted a cross-sectional study of SLE patients admitted to the emergency service at Hospital San Vicente Fundación (HSVF). The HSVF ethics committee approved the execution of the project.Patients were categorized into four groups according to the main cause of hospitalization: (1) infection, (2) flare, (3) infection and flare and, (4) neither infection nor flare.We calculated the median values of the ratios and their respective interquartile ranges for each group. Then, we compared those summary measures using the Kruskal-Wallis test. Subsequently, we assessed the predictive capacity of infection of each ratio using ROC curve. Finally, we carried out a logistic regression model.Results:A total of 246 patients were included, among them 90.7% were women. The median age was 28 years (IQR: 20-35 years). Regarding the outcomes, 37.0% of the patients had flares, 30.9% had neither infection nor flare, 16.7% had an infection and, 15.5% had simultaneously infection and flare. When compared the four groups, statistical significance (p<0.05) was observed. Area under the ROC curve (AUC) for infection prediction was as follows: 0.752 (sensitivity 60.5%, specificity 80.5%) for LC4R, 0.740 (sensitivity 73.2%, specificity 68.3%) for FER, 0.731 (sensitivity 77.6%, specificity 80.5%) for LC3R.In the logistic regression modeling, we observed that an increase in the risk of infection was associated with an LC4R below 66.7 (OR: 6.3, CI: 2.7 – 14.3, p <0.0001), a FER greater than 13.6 (OR: 5.9, CI: 2.8 – 12.1, p <0.0001) and an LC3R below 11.2 (OR: 4.9, CI: 2.4 – 9.8, p <0.0001).The ESR/CRP and PLR performed poorly with an AUC of 0.580 and 0.655, respectively. In contrast, the NLR showed better performance (AUC of 0.709, with a sensitivity of 80.2% and specificity of 55.7%).Figure 1.ROC curves of the evaluated ratiosConclusion:These laboratory ratios could be easy to assay and inexpensive biomarkers to differentiate between infection and activity in SLE patients. The LC4R, FER, and LC3R have a significant diagnostic performance for detecting infection among SLE patients. Of the ratios previously evaluated, ESR/CRP, LPR, NLR, only the latest has an adequate performance in our population.References:[1]Littlejohn E, Marder W, Lewis E, et al. The ratio of erythrocyte sedimentation rate to C-reactive protein is useful in distinguishing infection from flare in systemic lupus erythematosus patients presenting with fever. Lupus. 2018;27(7):1123-1129.[2]Broca-Garcia BE, Saavedra MA, Martínez-Bencomo MA, et al. Utility of neutrophil-to-lymphocyte ratio plus C-reactive protein for infection in systemic lupus erythematosus. Lupus. 2019;28(2):217-222.[3]Soliman WM, Sherif NM, Ghanima IM, EL-Badawy MA. Neutrophil to lymphocyte and platelet to lymphocyte ratios in systemic lupus erythematosus: Relation with disease activity and lupus nephritis. Reumatol Clin. 2020;16(4):255-261s.Disclosure of Interests:None declared

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Impact of Matrix Metalloproteinase-9 during Periodontitis and Cardiovascular Diseases

Molecules ◽

10.3390/molecules26061777 ◽

2021 ◽

Vol 26 (6) ◽

pp. 1777

Author(s):

Gaetano Isola ◽

Alessandro Polizzi ◽

Vincenzo Ronsivalle ◽

Angela Alibrandi ◽

Giuseppe Palazzo ◽

...

Keyword(s):

Matrix Metalloproteinase ◽

Healthy Subjects ◽

Multivariate Regression Analysis ◽

Matrix Metalloproteinase 9 ◽

C Reactive Protein ◽

Reactive Protein ◽

Hs Crp ◽

The Impact ◽

Negative Predictor ◽

Metalloproteinase 9

Matrix metalloproteinase-9 (MMP-9) has been shown to play a key role in endothelial function and perhaps pivotal in the correlation between periodontal disease and cardiovascular disease (CVD). For the study, the impact of MMP-9 of periodontitis and CVD on serum and saliva concentrations was analyzed. For the study patients with periodontitis (n = 31), CVD (n = 31), periodontitis + CVD (n = 31), and healthy patients (n = 31) were enrolled. Clinical and demographic characteristics as well as serum and salivary MMP-9 were evaluated. MMP-9 concentrations in serum and saliva were statistically elevated in patients with CVD (p < 0.01) and in patients with periodontitis plus CVD (p < 0.001) compared to patients with periodontitis and healthy subjects. Multivariate regression analysis showed that c-reactive protein (hs-CRP) was the only significant predictor for MMP-9 serum (p < 0.001), whereas hs-CRP (p < 0.001) and total cholesterol (p = 0.029) were the statistically significant salivary MMP-9 predictors. This study evidenced that patients with CVD and periodontitis + CVD presented elevated MMP-9 concentrations in serum and saliva compared to patients with periodontitis and healthy subjects. Furthermore, hs-CRP was a negative predictor of serum and salivary MMP-9.

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Observational Study of Lipid Profile and C-Reactive Protein after a Seven-Day Fast

Nutrients ◽

10.3390/nu13010255 ◽

2021 ◽

Vol 13 (1) ◽

pp. 255

Author(s):

Valeria Galetti ◽

Marica Brnic ◽

Benjamin Lotin ◽

Mauro Frigeri

Keyword(s):

Total Cholesterol ◽

Medical Center ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Blood Parameters ◽

Swiss Alps ◽

Risk Level ◽

Metabolic Risk ◽

C Reactive Protein ◽

Reactive Protein

Fasting is becoming an increasingly popular practice. Nevertheless, its clinical benefits and possible inconveniences remain limitedly evaluated. We observed the effects of a seven-day fast conducted in a non-medical center located in the Swiss Alps. Clinical parameters were measured on the first and last day of fasting (D1 and D7), and two months later (D60). Among the 40 participants, blood analyses were done on 25 persons with an increased metabolic risk, with the primary goal of assessing the lasting effect on low-density lipoprotein (LDL) cholesterol. By comparing D60 with D1, high-density lipoprotein cholesterol (HDL) (+0.15 mmol/L) and insulin-like growth factor-1 (IGF-1) (+2.05 mmol/L) increased (both p < 0.009), all other blood parameters (LDL, glucose, total cholesterol, triglycerides, C-reactive protein (CRP)) did not change; weight (−0.97 kg) and hearth rate (−7.31 min−1) decreased (both p < 0.006). By comparing D7 with D1, total cholesterol (+0.44 mmol/L), triglycerides (+0.37 mmol/L) and CRP (+3.37 mg/L) increased (all p < 0.02). The lack of LDL variation at D60 may be due to the low metabolic risk level of the participants. The increase of total cholesterol, triglycerides and CRP at D7 warrants studies to understand whether such fluctuations represent a stress reaction to the fasting state, which may vary in different fasting types.

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