scholarly journals Effect of 8 Weeks of High Intensity Interval Training (HIIT) With and Without Calorie Restriction on Gene Expression of Caspase-3 and Caspase-9 Proteins in Male Rats

2020 ◽  
Vol 23 (3) ◽  
pp. 300-313
Author(s):  
Mohammadreza Zarali ◽  
◽  
Zaher Etemad ◽  
Kamal Azizbeigi ◽  
Pouran Karimi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Exercise Training ◽  
Caloric Restriction ◽  
High Intensity ◽  
Caspase 3 ◽  
Interval Training ◽  
High Intensity Interval Training ◽  
Interval Exercise ◽  
High Intensity Interval ◽  
And Control

Background and Aim Apoptosis or programmed death is active biological process and reversible that the balance between growth and cell death in various tissues, particularly myocardial tissue is essential role. The aim of this study was the effect of 8 weeks High Intensity Interval Training (HIIT) with and without food restriction on gene expression of Caspase 3 and 9 in rats. Methods & Materials: This study was experimental multi group design with control group which conducted on 30 rats with two months old male. Subjects were divided in to five homogenous groups including base control, control, caloric restriction, interval exercise training and caloric restriction+interval exercise training. Training groups five sessions in weeks were participated in interval exercise training for 8 weeks. The level of gene expression of the proteins Caspase 3 and 9 was evaluated by Real Time-PCR. Dates were analyzed using the 1-way ANOVA at the level of (P<0.05). Ethical Considerations: The protocol of this study was approved by the Medical Committee of Sanandaj University of Medical Sciences (Code: IR.MYK.REC.1397.5022). Results: The results showed that the training group had a significant decrease in gene expression of Caspase 3 in comparison with caloric restriction+exercise training (P<0.05). Also, exercise training and exercise training+caloric restriction significantly decrease in the gene expression of Caspase 9 compared to caloric restriction, base control and control (P<0.05) and significantly decrease in the gene expression of Caspase 3 compared to base control and control (P<0.05). Conclusion: According to the results, it seems that high intensity interval training with and without calorie restriction provides the necessary adaptations to inhibit or stop apoptosis induced by aerobic exercise. 

scholarly journals Effect of 8 weeks high-intensity interval training (HIIT) with and without caloric restriction on gene expression of myocardial Bax and Bcl2 in mice

2021 ◽  
Vol 43 (1) ◽  
pp. 124-133
Author(s):  
Mohammadreza Zarali ◽  
Zaher Etemad ◽  
Kamal Azizbeigi ◽  
Pouran Karimi
Keyword(s):  
Gene Expression ◽  
Exercise Training ◽  
Caloric Restriction ◽  
High Intensity ◽  
Interval Training ◽  
Training Group ◽  
Control Group ◽  
High Intensity Interval Training ◽  
Interval Exercise ◽  
High Intensity Interval

Background: Apoptosis is the physiological cell death that in natural conditions leads to the elimination of old, damaged, waste, and harmful cells. The aim of this study was the effect of eight weeks of high-intensity interval training (HIIT) with and without caloric restriction on gene expression of myocardial Bax and Bcl2 in mice. Methods: Present study was an experimental multi-group design with a control group conducted on 30 two-month old male mice. Subjects were divided into five homogenous groups including base control, control, caloric restriction, interval exercise training, and caloric restriction + interval exercise training. Training groups participated in interval exercise training five sessions per week for 8 weeks. The level of gene expression of myocardial Bax and Bcl2 was evaluated by real-time PCR. Data were analyzed using the one-way ANOVA at the level of (P<0.05). Results: The results showed that the training group had a significant increase in gene expression of myocardial Bcl2 in comparison with caloric restriction + exercise training (P<0.05) and a significant decrease in gene expression of myocardial Bax compared to the caloric restriction group (P<0.05). Also, exercise training and exercise training + caloric restriction significantly increased the gene expression of myocardial Bcl2 and significantly decreased Bax/Bcl2 ratio compared to caloric restriction, base control, and control (P< 0.05). Conclusion: It seems that high-intensity interval training without caloric restriction would provide a suitable environment for increasing the integrity of the mitochondrial membrane of myocardial cells and possibly apoptosis.

scholarly journals Preconditioning Effect of High-Intensity Interval Training (HIIT) and Berberine Supplementation on the Gene Expression of Angiogenesis Regulators and Caspase-3 Protein in the Rats with Myocardial Ischemia-Reperfusion (IR) Injury

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Parisa Banaei ◽  
Farzad Nazem ◽  
Afshin Nazari ◽  
Arash Arjomand
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
High Intensity ◽  
Caspase 3 ◽  
Interval Training ◽  
High Intensity Interval Training ◽  
Related Factors ◽  
Significant Difference ◽  
High Intensity Interval ◽  
The Impact

Objective. It has been shown that angiogenesis is a desirable treatment for patients with ischemic heart disease. We set out to investigate the impact of high-intensity interval training (HIIT) and berberine supplementation on the gene expression of angiogenesis-related factors and caspase-3 protein in rats suffering from myocardial ischemic-reperfusion injury. Methods. Fifty rats were divided into the following groups: (1) trained, (2) berberine supplemented, (3) combined, and (4) IR. Each cohort underwent five sessions of HIIT per week for a duration of 8 weeks followed by induction of ischemia. Seven days after completion of reperfusion, changes in the gene expression of angiogenesis-related factors and caspase-3 protein were evaluated in the heart tissue. Results. We observed a significant difference between four groups in the transcript levels of vascular endothelial cell growth factor (VEGF), fibroblast growth factor-2 (FGF2), and thrombospondin-1(TSP-1) (p≤0.05). However, the difference in endostatin (ENDO) levels was not significant among the groups despite a discernible reduction (p≥0.05). Moreover, caspase-3 protein and infarct size were significantly reduced in the intervention groups (p≤0.05), and cardiac function increased in response to these interventions. Conclusion. The treatments exert their effect, likely, by reducing caspase-3 protein and increasing the expression of angiogenesis-promoting factors, concomitant with a reduction in inhibitors of the process.

scholarly journals Effects of Exercise Training on Anabolic and Catabolic Hormones with Advanced Age: A Systematic Review

2021 ◽  
Author(s):  
Hassane Zouhal ◽  
Ayyappan Jayavel ◽  
Kamalanathan Parasuraman ◽  
Lawrence D. Hayes ◽  
Claire Tourny ◽  
...  
Keyword(s):  
Systematic Review ◽  
Exercise Training ◽  
High Intensity ◽  
Aerobic Training ◽  
Training Programs ◽  
Interval Training ◽  
Sex Hormone Binding Globulin ◽  
High Intensity Interval Training ◽  
Interval Exercise ◽  
High Intensity Interval

Abstract Background Ageing is accompanied by decreases in physical capacity and physiological regulatory mechanisms including altered hormonal regulation compared with age-matched sedentary people. The potential benefits of exercise in restoring such altered hormone production and secretion compared to age-matched physically inactive individuals who are ageing remains unclear. Objectives The aim of this systematic review was to summarise the findings of exercise training in modulating levels of ostensibly anabolic and catabolic hormones in adults aged > 40 years. Methods We searched the following electronic databases (to July 2021) without a period limit: Cochrane Library, PubMed, Science Direct, Scopus, SPORTDiscus and Web of Science. Additionally, a manual search for published studies in Google Scholar was conducted for analysis of the ‘grey literature’ (information produced outside of traditional commercial or academic publishing and distribution channels). The initial search used the terms ‘ageing’ OR ‘advanced age’ OR ‘old people’ OR ‘older’ OR elderly’ AND ‘anabolic hormones’ OR ‘catabolic hormones’ OR ‘steroid hormones’ OR ‘sex hormones’ OR ‘testosterone’ OR ‘cortisol’ OR ‘insulin’ OR ‘insulin-like growth factor-1’ OR ‘IGF-1’ OR ‘sex hormone-binding globulin’ OR ‘SHBG’ OR ‘growth hormone’ OR ‘hGH’ OR ‘dehydroepiandrosterone’ OR ‘DHEA’ OR ‘dehydroepiandrosterone sulfate (DHEA-S)’ AND ‘exercise training’ OR ‘endurance training’ OR ‘resistance training’ OR ‘ strength training’ OR ‘weight-lifting’ OR ‘high-intensity interval training’ OR ‘high-intensity interval exercise’ OR ‘high-intensity intermittent training’ OR ‘high-intensity intermittent exercise’ OR ‘interval aerobic training’ OR ‘interval aerobic exercise’ OR ‘intermittent aerobic training’ OR ‘intermittent aerobic exercise’ OR ‘high-intensity training’ OR ‘high-intensity exercise’ OR ‘sprint interval training’ OR ‘sprint interval exercise’ OR ‘combined exercise training’ OR ‘anaerobic training’. Only eligible full texts in English or French were considered for analysis. Results Our search identified 484 records, which led to 33 studies for inclusion in the analysis. Different exercise training programs were used with nine studies using endurance training programs, ten studies examining the effects of high-intensity interval training, and 14 studies investigating the effects of resistance training. Most training programs lasted ≥ 2 weeks. Studies, regardless of the design, duration or intensity of exercise training, reported increases in testosterone, sex hormone-binding globulin (SHBG), insulin-like growth factor-1 (IGF-1), human growth hormone (hGH) or dehydroepiandrosterone (DHEA) (effect size: 0.19 < d < 3.37, small to very large) in both older males and females. However, there was no consensus on the effects of exercise on changes in cortisol and insulin in older adults. Conclusion In conclusion, findings from this systematic review suggest that exercise training increases basal levels of testosterone, IGF-1, SHBG, hGH and DHEA in both male and females over 40 years of age. The increases in blood levels of these hormones were independent of the mode, duration and intensity of the training programs. However, the effects of long-term exercise training on cortisol and insulin levels in elderly people are less clear.

Effects of high-intensity interval training and flaxseed oil supplement on learning, memory and immobility: relationship with BDNF and TrkB genes

2020 ◽  
pp. 1-12
Author(s):  
S. Rahmati-Ahmadabad ◽  
M.-A. Azarbayjani ◽  
D.R. Broom ◽  
M. Nasehi
Keyword(s):  
Gene Expression ◽  
Exercise Training ◽  
Executive Functions ◽  
High Intensity ◽  
Interval Training ◽  
Flaxseed Oil ◽  
High Intensity Interval Training ◽  
Positive Effects ◽  
High Intensity Interval ◽  
Positive Effect

This study examined the independent and combined effects of high-intensity interval training (HIIT) and flaxseed oil supplementation on cognitive/executive functions in middle-aged rats. Hippocampal neurotropic brain factor (BDNF) and tyrosine kinase receptor B (TrkB) gene expression were also measured. Animals were randomly divided into groups including no exercise control and saline (CS), no exercise control and flaxseed oil supplement (CF), exercise training-and saline (TS) and exercise training and flaxseed oil supplement (TF). The training groups undertook a program of HIIT (10 weeks, five sessions per week) and the supplement groups received flaxseed oil supplement (300 mg/kg). The results showed that HIIT and flaxseed oil supplementation independently had positive effects on memory and learning (P<0.05). HIIT and flaxseed oil independently decreased immobility behaviour and increased hippocampal BDNF and TrkB genes expression (P<0.05). HIIT and flaxseed oil combination had a greater effect on some variables (hippocampal TrkB gene expression, memory and immobility) compared to each intervention alone (P<0.05). In conclusion, HIIT and flaxseed oil can independently improve cognitive/executive functions. In addition, HIIT had a greater positive effect than flaxseed oil supplementation on memory and immobility. The combination of HIIT and flaxseed oil supplement had a more positive effect compared to each intervention alone on memory, and immobility. Hippocampal BDNF gene expression did not significantly differ in the combination or independent groups. Thus, future work is needed on several other genes in different segments of the brain to find the additive-mechanisms involved in memory and immobility regulation and younger and older species of rat should be examined.

Effects of 4-week small-sided games vs. high-intensity interval training with changes of direction in female collegiate basketball players

2021 ◽  
pp. 174795412110327
Author(s):  
Jinshu Zeng ◽  
Jing Xu ◽  
Yuanhong Xu ◽  
Wu Zhou ◽  
Fei Xu
Keyword(s):  
Physical Performance ◽  
High Intensity ◽  
Perceived Exertion ◽  
Interval Training ◽  
High Intensity Interval Training ◽  
Basketball Players ◽  
Collegiate Basketball ◽  
High Intensity Interval ◽  
And Control ◽  
Movement Test

The aim of the study was to investigate the effects of 4-week small-sided games (SSG) and high-intensity interval training with changes of direction (HIT-COD) on physical performance and specific technical skills in female collegiate basketball players. Nineteen players were divided into SSG (n = 9) and HIT-COD (n = 10) groups, that performed either SSG or HIT-COD three times per week for 4 weeks during the pre-season. Players’ heart rate (HR) and perceived exertion responses (RPE) were assessed during the intervention. Before and after the intervention period, performances were assessed with 30-15 intermittent fitness test (30-15IFT), repeated sprint ability (RSA) test, modified agility T-test (MAT), countermovement jump (CMJ), 20-m sprint, shooting accuracy test, 1 min shooting test, passing test, defensive movement test and control dribble test. Both training interventions led to similar physiological and perceived exertion responses, showing no significant differences in HR ( P = .49, d = 0.2) and RPE ( P = .77, d = 0.1) between groups. Significant improvements were observed in 30-15IFT (SSG: 4.1%, d = 1.5; HIT-COD: 4.2%, d = 1.7), RSAmean (SSG: −2.2%, d = 1.0; HIT-COD: −1.9%, d = 1.0), RSAbest (SSG: −2.0%, d = 0.9; HIT-COD: −2.1%, d = 1.1), MAT (SSG: −7.2%, d = 1.7; HIT-COD: 5.7%, d = 1.5), defensive movement test (SSG: −5.1%, d = 2.1; HIT-COD: −5.8%, d = 1.8) and control dribble test (SSG: −3.4%, d = 1.0; HIT-COD: −2.6%, d = 1.0). The only significant group × time interaction was found ( P = .032, [Formula: see text] = 0.24), with SSG improving 1 min shooting (22.4%, d = 1.0) and HIT-COD performing slightly worse (−2.6%, d = 0.1) after a 4-week intervention. The current study suggests that using SSG is more effective than HIT-COD for female collegiate basketball players in pre-season, since SSG improves physical performance and basketball-specific movements as well as shooting abilities after a 4-week intervention.

High intensity interval training reduces systemic inflammation in post-PCI patients

2011 ◽  
Vol 18 (6) ◽  
pp. 850-857 ◽  
Author(s):  
Peter Scott Munk ◽  
Unni Mathilde Breland ◽  
Pål Aukrust ◽  
Thor Ueland ◽  
Jan Terje Kvaløy ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Exercise Training ◽  
Stable Angina ◽  
High Intensity ◽  
Inflammatory Markers ◽  
Cell Activation ◽  
Interval Training ◽  
High Intensity Interval Training ◽  
Endothelial Cell Activation ◽  
High Intensity Interval

Background: Increased plasma levels of inflammatory markers and markers of endothelial cell activation have been associated with increased risk for cardiovascular events. Exercise training may lower the risk for coronary heart disease by attenuating inflammation and improving endothelial function. The objective of this study was to evaluate effects of regular high-intensity exercise training on a wide range of markers of inflammation and endothelial cell activation. Materials and methods: Consecutively, 40 patients were prospectively randomized to a 6 months supervised high-intensity interval training programme or to a control group following successful percutaneous coronary intervention (PCI). Blood samples of 36 patients with stable angina, drawn at baseline (before PCI) and at 6 months, were analysed. Late luminal loss was measured at 6 months using quantitative coronary angiography. Results: At 6 months, levels of the inflammatory markers interleukin (IL)-6 and IL-8 were reduced and levels of the anti-inflammatory cytokine IL-10 increased in the training group only. The decrease in IL-6 and C-reactive protein levels were significantly correlated with the decrease in luminal loss following PCI. In contrast to these anti-inflammatory effects, training had no effect on markers of platelet-mediated inflammation, and the effect of training on markers on endothelial cell activation were rather complex showing attenuating (von Willebrand factor) and enhancing (E-selectin and vascular cell adhesion molecule 1) effects. Conclusions: Regular exercise training in stable angina patients following PCI may attenuate some, but not all, inflammatory pathways, potentially contributing to the beneficial effects of exercise training on restenosis.

scholarly journals High-intensity interval training modulates retinal microvascular phenotype and DNA methylation of p66Shc gene: a randomized controlled trial (EXAMIN AGE)

2019 ◽  
Vol 41 (15) ◽  
pp. 1514-1519 ◽  
Author(s):  
Lukas Streese ◽  
Abdul Waheed Khan ◽  
Arne Deiseroth ◽  
Shafaat Hussain ◽  
Rosa Suades ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Dna Methylation ◽  
High Intensity ◽  
Interval Training ◽  
Retinal Vessel ◽  
High Intensity Interval Training ◽  
Age Related ◽  
High Intensity Interval ◽  
Retinal Arteriolar

Abstract Aims Impairments of retinal vessel diameter are associated with major adverse cardiovascular (CV) events. Promoter DNA methylation is a repressor of the mitochondrial adaptor p66Shc gene transcription, a key driver of ageing-induced reactive oxygen species. The study aimed to investigate whether high-intensity interval training (HIIT) affects retinal microvascular phenotype as well as p66Shc expression and oxidative stress in ageing subjects with increased CV risk from the EXAMIN AGE cohort. Methods and results Eighty-four sedentary subjects (mean age 59.4 ± 7.0 years) with ≥2 CV risk factors were randomized into either a 12-week HIIT or standard physical activity recommendations. Retinal arteriolar and venular diameters were measured by use of a retinal vessel analyser. As a marker of oxidative stress plasma 3-nitrotyrosine (3-NT) level was determined by ELISA. Gene expression of p66Shc and DNA methylation were assessed in mononuclear cells by RT-qPCR and methylated-DNA capture (MethylMiner Enrichment Kit) coupled with qPCR, respectively. High-intensity interval training reduced body mass index, fat mass, low-density lipoprotein and increased muscle mass, as well as maximal oxygen uptake (VO2max). Moreover, HIIT restored microvascular phenotype by inducing retinal arteriolar widening (pre: 175 ± 14 µm vs. post: 181 ± 13 µm, P = 0.001) and venular narrowing (pre: 222 ± 14 µm vs. post: 220 ± 14 µm, P = 0.007). After HIIT, restoration of p66Shc promoter methylation (P = 0.034) reduced p66Shc gene expression (P = 0.037) and, in turn, blunted 3-NT plasma levels (P = 0.002). Conclusion High-intensity interval training rescues microvascular dysfunction in ageing subjects at increased CV risk. Exercise-induced reprogramming of DNA methylation of p66Shc gene may represent a putative mechanistic link whereby exercise protects against age-related oxidative stress. Clinical trial registration  ClinicalTrials.gov: NCT02796976 (https://clinicaltrials.gov/ct2/show/NCT02796976).

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