KINERJA MIKROBA Zymomonas mobilis DAN Saccharomyces cerevisiae UNTUK MENGURAIKAN HIDROLISAT TONGKOL JAGUNG MENJADI BIOETANOL DENGAN PENGARUH WAKTU FERMENTASI DAN RASIO PENAMBAHAN MIKROBA

Bioethanol from biomass is one of energy which has a potential as alternative fuel. Bioethanol can be produced by using fungi or bacteria. The research was about the performance of Zymomonas mobilis and Saccharomyces cerevisiae to change corn cobs hydrolyzate into bioethanol by adding microbes to the influence of time and ratio of fermentation had been done. The hydrolyzate were decomposition of corn cobs using Trichoderma reesei and Aspergillus niger. The purpose of this study was to know the conversion of hydrolysis of corn cobs into bioethanol with variation time of fermentation (1 day, 3 days, 5 days, and 7 days) and rate of adding microbes (Zymomonas mobilis : Saccharomyces cerevisiae = 1:1; 1:2 and 2:1) (v/v). The glucose from corn cobs hydrolyzate was 5,869 g/ml. Fermentation wass carried out at 25 0C. Bioethanol which obtained from this study was investigated using gas chromatography. The optimum bioethanol yield was equal to 6,31% by using Zymomonas mobilis : Saccharomyces cerevisiae (2:1) and at the 3 days fermentation time.

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Utilization of robusta coffee waste as a renewable energy material - bioetanol

MATEC Web of Conferences ◽

10.1051/matecconf/201815401004 ◽

2018 ◽

Vol 154 ◽

pp. 01004

Author(s):

Sutarno ◽

Abdul Malik Kholiq

Keyword(s):

Saccharomyces Cerevisiae ◽

Renewable Energy ◽

Fermentation Process ◽

Fermentation Time ◽

Coffee Waste ◽

Robusta Coffee ◽

Energy Material ◽

Catalyst Type ◽

Hydrolysis Of

A research on robusta coffee waste has been conducted as a renewable energy material - Bioethanol. This research was carried out by hydrolysis and fermentation process using Zymomonasmobilis and Saccharomyces cerevisiae (Zymomonasmobilis) bacteria to obtain the best catalyst type in the process of hydrolysis of coffee skin to glucose and the effect of fermentation time on bioethanol content produced. This research was conducted by varying the fermentation time of 7 days; 8 days; 9 days and 10 days. The fermentation fluid was then distilled and tested for bioethanol using a refractometer. Furthermore, bioethanol concentration in the analysis using.

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Simultaneous Saccharification and Fermentation of Corn Cobs to Bio-Ethanol by Co-Culture of Aspergillus Niger and Saccharomyces Cerevisiae

International Journal of Environmental Science and Development ◽

10.7763/ijesd.2013.v4.343 ◽

2013 ◽

pp. 239-242 ◽

Cited By ~ 10

Author(s):

J. Itelima ◽

A. Ogbonna ◽

S. Pandukur ◽

J. Egbere ◽

A. Salami

Keyword(s):

Saccharomyces Cerevisiae ◽

Aspergillus Niger ◽

Simultaneous Saccharification And Fermentation ◽

Corn Cobs ◽

Simultaneous Saccharification

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Ethanol production from corn cobs by co-culture of Saccharomyces cerevisiae and Aspergillus niger

Bayero Journal of Pure and Applied Sciences ◽

10.4314/bajopas.v2i2.63792 ◽

2011 ◽

Vol 2 (2) ◽

Author(s):

SA Ado ◽

GU Kachalla ◽

MB Tijjani ◽

MS Aliyu

Keyword(s):

Saccharomyces Cerevisiae ◽

Aspergillus Niger ◽

Ethanol Production ◽

Corn Cobs

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Lignocellulosic Processing with Acid Pretreatment and Enzymatic Hydrolysis for Improving the Acquisition of Sugar Fermentation

Modern Applied Science ◽

10.5539/mas.v9n7p23 ◽

2015 ◽

Vol 9 (7) ◽

pp. 23 ◽

Cited By ~ 1

Author(s):

Nuniek Hendrianie ◽

Sri Rahmania Juliastuti ◽

Moch. Izati Iwani ◽

Affrida Eka

Keyword(s):

Saccharomyces Cerevisiae ◽

Aspergillus Niger ◽

Trichoderma Reesei ◽

Lignin Content ◽

Banana Peel ◽

Acid Pretreatment ◽

Glucose Content ◽

Mixture Ratio ◽

Glucose Levels ◽

Sugar Fermentation

Banana peels is a waste which has not been widely used, so that the relatively long time the existence of such waste brings its own problems such as pollution. Banana peels contain high enough lignocellulosic and can be degraded into simpler forms. The Lignin content of banana peels needs to be removed / destroyed structure. The purpose of this study was to determine the effect of the addition ratio of Trichoderma reseei and Aspergillus niger on hydrolysis liquefaction and saccharification time in stage hydrolysis to glucose produced with the highest glucose parameters. In this study, the method was used to degrade lignin by using sulfuric acid (2%). Afterward, the cellulose content was changed in liquefaction process. Hydrolysis liquefaction performed using Aspergillus niger and Trichoderma reesei with a mixture ratio of 1: 0, 0: 1, 1: 1 and 1: 2. Furthermore, substrate hydrolysis liquefaction saccharification going through the process of hydrolysis to increase glucose levels were formed. Hydrolysis liquefaction aims to change the content of starch in the banana skin into glucose using Saccharomyces cerevisiae as the addition of 20% (v / v). Hydrolysis process of liquefaction is done by varying the sampling time on day - 3, 6, and 9. In this study the hydrolysis of liquefaction of banana peels use a mixture of Trichoderma reesei and Aspergillus niger 2:1 at a temperature of 50oC, pH 5, and 64 h for resulted in glucose with the best content was 0.52%. In addition, the results showed that the hydrolysis saccharification with the addition of Saccharomyces cerevisiae glucose levels were highest on day 3 with a temperature of 30oC and pH 5, resulting in glucose content to 1.63%.

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Production of Bio-Ethanol via Hydrolysis and Fermentation Using Cassava Peel and Used Newspaper as Raw Materials

Materials Science Forum ◽

10.4028/www.scientific.net/msf.981.222 ◽

2020 ◽

Vol 981 ◽

pp. 222-227

Author(s):

Tintin Mutiara ◽

Siska Widiawati ◽

Syafira Rachmatyah ◽

Achmad Chafidz

Keyword(s):

Saccharomyces Cerevisiae ◽

Energy Source ◽

Renewable Energy Source ◽

Gas Chromatography ◽

Manihot Esculenta ◽

Raw Materials ◽

Solid Wastes ◽

Waste Materials ◽

Fermentation Time ◽

Cassava Peel

Cassava peel and used newspaper are example of daily solid wastes that continue to increase in Indonesia from year to year. Most of people in Indonesia have not utilized cassava (Manihot Esculenta L.) peel and used newspaper properly, hence they only become untreated wastes. Cassava peel highly contains carbohydrate and starch which is about 44 - 59%, while newsprint contains quite a lot of cellulose which is 61%, so that both wastes have the potential to make bio-ethanol as a one of potential renewable energy source. The focus of this study is to produce bio-ethanol from these two waste materials. There were several stages to produce bio-ethanol, which are hydrolysis with 0.1 N hydrochloric acid (HCl), and fermentation with yeast (Saccharomyces Cerevisiae), and separation of the bio-ethanol by distillation. The content of bio-ethanol produced was analyzed by Gas Chromatography (GC). The bio-ethanol was produced under different variables, which are: amount of yeast (8 g and 10 g), fermentation time (3, 7, and 10 days), and ratio of volume of solution (mL) cassava peel waste : used newspaper (i.e. 80:20; 60:40; 50:50; 40:60). The GC analysis results showed that the bio-ethanol contents were approximately 0%, 6.194%, 8.421% and 8.887% for ratio volume of solution (mL) cassava peel waste : used newspaper of 80:20; 60:40; 50:50; 40:60, respectively. Whereas, the bio-ethanol contents for 3, 7, and 10 days of fermentation time were 6.690%, 8.765%, and 9.472%, respectively. In this study, the highest bio-ethanol content produced (i.e. 9.472%) was in the following variables: amount of yeast 10 g, 10 days fermentation time, ratio volume of solution (mL) cassava peel waste : used newspaper of 50:50.

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Optimization of enzymatic hydrolysis of steam-exploded corn stover by two approaches: Response surface methodology or using cellulase from mixed cultures of Trichoderma reesei RUT-C30 and Aspergillus niger NL02

Bioresource Technology ◽

10.1016/j.biortech.2010.01.078 ◽

2010 ◽

Vol 101 (11) ◽

pp. 4111-4119 ◽

Cited By ~ 80

Author(s):

Hao Fang ◽

Chen Zhao ◽

Xiang-Yang Song

Keyword(s):

Response Surface Methodology ◽

Enzymatic Hydrolysis ◽

Aspergillus Niger ◽

Response Surface ◽

Corn Stover ◽

Trichoderma Reesei ◽

Mixed Cultures ◽

Trichoderma Reesei Rut C30 ◽

Hydrolysis Of ◽

Rut C30

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Ethanol fermentation of enzymatically hydrolysed pretreated wood fractions using Trichoderma cellulases, Zymomonas mobilis, and Saccharomyces cerevisiae

Canadian Journal of Microbiology ◽

10.1139/m82-196 ◽

1982 ◽

Vol 28 (12) ◽

pp. 1311-1319 ◽

Cited By ~ 40

Author(s):

J. N. Saddler ◽

C. Hogan ◽

M. K.-H. Chan ◽

G. Louis-Seize

Keyword(s):

Saccharomyces Cerevisiae ◽

Cell Cultures ◽

Ethanol Fermentation ◽

Zymomonas Mobilis ◽

Protein A ◽

A Value ◽

Culture Filtrates ◽

Rate Of Hydrolysis ◽

Pretreatment Conditions ◽

Hydrolysis Of

Several highly cellulolytic fungi were initially screened for their cellulase and β-glucosidase activities. Culture filtrates from Trichoderma E58 demonstrated the highest β-glucosidase activity, giving a value of 1.0 IU/mg protein. A variety of different cellulose and pretreated wood substrates were hydrolysed by various cellulase preparations. The importance of the pretreatment conditions and ensuing chemical extractions of the cellulosic substrates was demonstrated by the range of sugar and ethanol values obtained after saccharification and fermentation of the liberated sugars. The rate of hydrolysis of the cellulosic substrate by Trichoderma E58 culture filtrates, concentrated culture filtrates, and whole cell cultures was compared. An ethanol value of 2.2% (w/v) could be obtained after hydrolysis of 5% Solka floe by concentrated culture filtrates of Trichoderma E58 and fermentation of the liberated sugars by Zymomonas mobilis or Saccharomyces cerevisiae.

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Bioethanol Levels of Dragon Fruit (Hylocereus polyrhizus) Peel with the Addition of Blend Crude Cellulase Enzyme from Trichoderma reesei and Aspergillus niger

Journal of Tropical Biodiversity and Biotechnology ◽

10.22146/jtbb.52189 ◽

2020 ◽

Vol 5 (1) ◽

pp. 1

Author(s):

Trianik Widyaningrum ◽

Masreza Parahadi

Keyword(s):

Aspergillus Niger ◽

Trichoderma Reesei ◽

Alternative Energy ◽

Cellulose Degradation ◽

Fruit Peel ◽

Yeast Saccharomyces Cerevisiae ◽

Fermentation Time ◽

Dragon Fruit ◽

Sugar Levels ◽

Hylocereus Polyrhizus

The petroleum fuel crisis shows that Indonesia's fossil energy reserves are limited. It is necessary to develop an environmentally, friendly and sustainable alternative energy, one of which is bioethanol. This study aims to determine the bioethanol levels of dragon fruit (Hylocereus polyrhizus) peel with the treatment of cellulase enzymes from Trichoderma reesei and Aspergillus niger. This research was an experimental study that uses steps such as making dragon fruit peel substrate and filtrate, cellulose degradation with enzymes from Trichoderma reesei and Aspergillus niger and inoculating with yeast (Saccharomyces cerevisiae) with a fermentation time of 96 hours and then measured reducing sugar levels with the method of DNS, distillation, and the measurement of bioethanol levels using alcohol meters. The results have shown that using enzymes from Trichoderma reesei and Aspergillus niger can increase the reduction of 49.68 % sugar levels in the treatment of T.reesei: A.niger (3: 1) and produce the highest bioethanol level, which is 2.46 % in the treatment of T.reesei: A.niger (2: 1)

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PENGARUH WAKTU FERMENTASI TERHADAP AKTIVITAS Saccharomyces cerevisiae DALAM MENGHIDROLISIS ECENG GONDOK (Eichhornia crassipes) (Effect of fermentation time activity in Saccharomyces cerevisiae Hydrolysis of Water Hyacinth (Eichhornia crassipes))

Jurnal Penelitian Saintek ◽

10.21831/jps.v26i2.43983 ◽

2021 ◽

Vol 26 (2) ◽

Author(s):

Purbowatiningrum Ria Sarjono ◽

Nies Suci Mulyani ◽

Ina Noprastika ◽

Ismiyarto Ismiyarto ◽

Ngadiwiyana Ngadiwiyana ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Water Hyacinth ◽

Eichhornia Crassipes ◽

Ph Optimum ◽

Time Activity ◽

Fermentation Time ◽

Hydrolysis Of

AbstrakSaccharomyces cerevisiae merupakan khamir yang berpotensi memproduksi enzim selulase yang mampu menghidrolisis ikatan β-1,4-glikosida pada rantai selulosa untuk menghasilkan glukosa. Salah satu sumber selulosa yang mudah ditemukan dan melimpah di alam adalah eceng gondok. Glukosa yang dihasilkan dari proses hidrolisis dapat dimanfaatkan untuk produksi sirup gula, asam organik dan bioetanol. Penelitian ini bertujuan untuk mendapatkan Saccharomyces cerevisiae yang diadaptasikan pada media fermentasi eceng gondok dan memperoleh data kadar gula pereduksi dari aktivitas Saccharomyces cerevisiae dalam menghidrolisis eceng gondok pada variasi pH dan waktu fermentasi. Proses yang terlebih dahulu dilakukan terhadap eceng gondok adalah delignifikasi dengan NaOH. Saccharomyces cerevisiae diadaptasikan dalam media modifikasi eceng gondok sebagai sumber karbon. Kurva pertumbuhan Saccharomyces cerevisiae dibuat dalam media modifikasi eceng gondok untuk mengetahui waktu optimum pertumbuhan Saccharomyces cerevisiae dalam menghasilkan enzim selulase, sehingga dapat mengetahui fase eksponensial dari Saccharomyces cerevisiae. Berdasarkan penelitian diperoleh hasil bahwa Saccharomyces cerevisiae dapat tumbuh pada media fermentasi eceng gondok dan Kadar gula pereduksi tertinggi dari aktivitas Saccharomyces cerevisiae dalam menghidrolisis eceng gondok pada pH optimum 5 dan waktu fermentasi optimum dalam menghasilkan gula pereduksi pada jam ke-48 sebesar 0,267 mg/L dari 0,3 gram eceng gondok kering.Kata kunci: Saccharomyces cerevisiae, hidrolisis selulosa, pH dan waktu fermentasi

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Microbial oil production byMortierella isabellinafrom corn stover under different pretreatments

RSC Advances ◽

10.1039/c7ra11900c ◽

2017 ◽

Vol 7 (89) ◽

pp. 56239-56246 ◽

Cited By ~ 4

Author(s):

Chen Zhao ◽

Lu Deng ◽

Hao Fang ◽

Shaolin Chen

Keyword(s):

Enzymatic Hydrolysis ◽

Aspergillus Niger ◽

Mixed Culture ◽

Corn Stover ◽

Trichoderma Reesei ◽

Oil Production ◽

Cellulase Production ◽

Microbial Oil ◽

Pretreated Corn Stover ◽

Hydrolysis Of

Mixed culture ofTrichoderma reeseiandAspergillus nigerwas employed to accomplish on-site cellulase production where cellulases were applied directly to the enzymatic hydrolysis of pretreated corn stover.

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