Malaria Detection using Polymerase Chain Reaction (PCR) Method in Pregnant Women’s Saliva on Several Hospitals in North Sulawesi Province

Indonesian Journal of Obstetrics and Gynecology ◽

10.32771/inajog.v36i1.279 ◽

2016 ◽

Author(s):

Dewanto Khrisnamurti

Keyword(s):

Pregnant Women ◽

First Trimester ◽

Cross Sectional Study ◽

Malaria In Pregnancy ◽

Cross Sectional ◽

North Sulawesi ◽

Blood Smears ◽

Pcr Method ◽

Polymerase Chain ◽

In Pregnancy

Objective: To detect malaria by PCR examination of saliva in pregnant women and to obtain the incidence of malaria and the type of plasmodium causing malaria in pregnant women at various hospital in North Sulawesi. Method: A descriptive cross sectional study in pregnant women during antenatal care at the Department of Obstetrics and Gynecology Faculty of Medicine University of Sam Ratulangi/Prof. Dr. R.D. Kandou General Hospital in Manado, R.W. Monginsidi Hospital in Manado, Bethesda Hospital in Tomohon, and Datoe Binangkang Hospital in Kotamobagu, from 1 April until 31 May 2008. Result: There were 43 pregnant women clinically diagnosed with malaria, 23 (53.49%) by PCR examination of saliva and 20 (46.59%) by blood smears. From 23 cases of malaria in pregnancy detected by PCR, there were 18 diagnosed as tropical malaria, 3 tertian malaria, and 2 mixed malaria. Conclusions: The incidence of malaria in pregnancy at various hospital in North Sulawesi using PCR methods for saliva examination from April 1 - May 31 2008 is 53.49%. In this study malaria are mostly caused by Plasmodium falciparum with the largest incidence in primigravida, in the first trimester. [Indones J Obstet Gynecol 2012; 36-1:14-9] Keywords: malaria, PCR, pregnancy, saliva

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Cross-sectional study to assess etiology and associated factors for anaemia during first trimester of pregnancy in Anuradhapura District, Sri Lanka: a protocol

F1000Research ◽

10.12688/f1000research.28226.1 ◽

2021 ◽

Vol 10 ◽

pp. 223

Author(s):

Gayani Amarasinghe ◽

Vasana Mendis ◽

Thilini Agampodi

Keyword(s):

Sri Lanka ◽

Data Collection ◽

Pregnant Women ◽

Associated Factors ◽

First Trimester ◽

Cross Sectional Study ◽

Sectional Study ◽

Cross Sectional ◽

Anaemia In Pregnancy ◽

In Pregnancy

Background: Anaemia in pregnancy, which can lead to adverse maternal and fetal outcomes, is a significant global health problem. Despite Sri Lanka’s strong public health system and commitment towards prevention, maternal anaemia remains a major problem in the country. While prevention is focused on iron deficiency, detailed etiological studies on this topic are scarce. Moreover, estimates of socio demographic and economic factors associated with anaemia in pregnancy, which can provide important clues for anaemia control, are also lacking. This study aims to evaluate the hemoglobin distribution, spatial distribution, etiology and associated factors for anaemia in pregnant women in Anuradhapura, Sri Lanka. Methods: This is a cross sectional study of pregnant women in their first trimester registered for antenatal care from July to September 2019 in the Anuradhapura district. The minimal sample size was calculated to be 1866. Initial data collection has already been carried out in special field clinics for pregnant women between June to October 2019. An interviewer-administered questionnaire, a self-completed dietary questionnaire and an examination checklist were used for data collection. In addition, all participants underwent complete blood count testing. Further investigations are being conducted for predicting the etiology of anaemia based on a developed algorithm (such as high-performance liquid chromatography [HPLC] and peripheral blood film analysis). Discussion: Being the largest study on anaemia during pregnancy in a single geographical area in Sri Lanka, this study will provide important clues about geographical clustering of anaemia cases with similar etiology, associated factors and etiologies which would help to develop interventions to improve the health of pregnant women in the area. The possibility of selection bias is a potential limitation associated with the study design.

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Malaria in Pregnancy in Endemic Regions of Colombia: High Frequency of Asymptomatic and Peri-Urban Infections in Pregnant Women with Malaria

Infectious Diseases in Obstetrics and Gynecology ◽

10.1155/2020/2750258 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10

Author(s):

Ana-María Vásquez ◽

Lina Zuluaga-Idárraga ◽

Margarita Arboleda ◽

Luz-Yáned Usuga ◽

Carolina Gallego-Marin ◽

...

Keyword(s):

Antenatal Care ◽

Pregnant Women ◽

Cross Sectional Study ◽

Malaria In Pregnancy ◽

Limited Information ◽

Cross Sectional ◽

History Of ◽

Placental Blood ◽

Adverse Pregnancy ◽

In Pregnancy

Background. Malaria in pregnancy (MiP) has been associated with adverse pregnancy outcomes. There is limited information on MiP in low transmission regions as Colombia. This study aimed to describe the epidemiology of MiP through active surveillance of infections by microscopy and polymerase chain reaction (PCR). Methods. A cross-sectional study was conducted between May 2016 and January 2017 in five municipalities (Apartadó, Turbo, El Bagre, Quibdó, and Tumaco) in Colombia. Pregnant women self-presenting at health centers for antenatal care visits, seeking medical care for suspected malaria, or delivery, were enrolled. Diagnosis of Plasmodium spp was made in peripheral and placental blood samples by microscopy and PCR. Results. A total of 787 pregnant women were enrolled; plasmodial infection was diagnosed by microscopy in 4.2% (95% CI 2.8-5.6; 33/787) or by nPCR in 5.3% (95% CI 3.8-6.9; 42/787) in peripheral blood. Most of the infections were caused by P. falciparum (78.5%), and 46% were afebrile (asymptomatic). Women in the first and second trimester of pregnancy were more likely to be infected (aOR=3.06, 95%CI=1.6−5.8). To live in the urban/peri-urban area (aOR=3.04, 95%CI=1.4−6.56), to have a history of malaria during last year (aOR=5.45, 95%IC=2.16−13.75), and the infrequent bed net usage (aOR=2.8, 95%CI=1.31−5.97) were associated with the infection. Pregnant infected women had a higher risk of anaemia (aOR=2.18, 95%CI=1.15−4.12) and fever (aOR=14.2, 95%CI=6.89−29.8). Conclusion. The screening for malaria during antenatal care in endemic areas of Colombia is highly recommended due to the potential adverse effects of Plasmodium spp. infection in pregnancy and as an important activity for the surveillance of asymptomatic infections in the control of malaria.

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Preventive and Curative treatment of malaria during pregnancy in Mali: Evaluation of the Healthcare Professionals based on the Malian National Malaria Control Program (NMCP) Guidelines

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v11i5-s.5092 ◽

2021 ◽

Vol 11 (5-S) ◽

pp. 71-76

Author(s):

Mahamadou BALLO ◽

Karim Traoré ◽

Samou Sidibé ◽

Seidina Diakité ◽

Abdoulaye Guindo ◽

...

Keyword(s):

Pregnant Women ◽

Malaria Transmission ◽

Control Program ◽

Preventive Treatment ◽

First Trimester ◽

Cross Sectional Study ◽

Antimalarial Drugs ◽

Third Trimester ◽

Cross Sectional ◽

In Pregnancy

Malaria infections in pregnancy should be treated promptly with safe and efficacious antimalarial drugs to prevent harmful effects on the mother and fetus. To succeed, the Malian has developed NMCP guidelines for the management of malaria cases in pregnant women. The study aimed at the analysis of the prescription of antimalarial drugs based on the Mali's NMCP guidelines. We conducted a cross-sectional study during malaria transmission season from June to August 2020. The sampling concerned all prescriptions for pregnant women containing at least one antimalarial drug. The frequency of prescription of antimalarial drugs was 85%. 132 (74.16%) were preventive treatments and 46 (25.84%) curative treatments. 30 (90.91%) of pregnant women in the first trimester received one dose of Sulfadoxine-Pyrimethamine. 6 (12.5%) received three doses in the third trimester. Of the 46 antimalarial drugs prescribed for the treatment of uncomplicated malaria, 30 (65.22%) were Artemether-lumefantrine (tablet), 10 (21.74%) were Quinine (tablet). 29 (63.04%) were compliant with NMCP guidelines and 17 (36.96%) were not. The non-compliances concerned 3 prescriptions of Artemether-lumefantrine in the first trimester, 3 and 5 prescriptions of Quinine (tablet) in the second and third trimester respectively and at the end 2 and 4 non-compliances respectively for the prescription of injectable dosage forms of Quinine and Artesunate. This study showed a great noncompliance with the Mali's NMCP guidelines in the prescription of antimalarial in pregnant women. Chemoprophylaxis should be prohibited in the first trimester. Keywords: Curative and Preventive Treatment, Malaria in Pregnancy, Malaria Transmission, Mali

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Cross-sectional study to assess etiology and associated factors for anaemia during first trimester of pregnancy in Anuradhapura District, Sri Lanka: a protocol

F1000Research ◽

10.12688/f1000research.28226.2 ◽

2021 ◽

Vol 10 ◽

pp. 223

Author(s):

Gayani Amarasinghe ◽

Vasana Mendis ◽

Thilini Agampodi ◽

Suneth Agampodi

Keyword(s):

Sri Lanka ◽

Data Collection ◽

Pregnant Women ◽

Associated Factors ◽

First Trimester ◽

Cross Sectional Study ◽

Sectional Study ◽

Cross Sectional ◽

Anaemia In Pregnancy ◽

In Pregnancy

Background: Anaemia in pregnancy, which can lead to adverse maternal and fetal outcomes, is a significant global health problem. Despite Sri Lanka’s strong public health system and commitment towards prevention, maternal anaemia remains a major problem in the country. While prevention is focused on iron deficiency, detailed etiological studies on this topic are scarce. Moreover, estimates of socio demographic and economic factors associated with anaemia in pregnancy, which can provide important clues for anaemia control, are also lacking. This study aims to evaluate the hemoglobin distribution, and geographical distribution, contribution of known aetiologies and associated factors for anaemia in pregnant women in Anuradhapura, Sri Lanka. Methods: This is a cross sectional study of pregnant women in their first trimester registered for antenatal care from July to September 2019 in Anuradhapura district. The minimal sample size was calculated to be 1866. Initial data collection has already been carried out in special field clinics for pregnant women between June to October 2019. An interviewer-administered questionnaire, a self-completed dietary questionnaire and an examination checklist were used for data collection. In addition, all participants underwent complete blood count testing. Further investigations are being conducted for predicting the etiology of anaemia based on a developed algorithm (such as high-performance liquid chromatography [HPLC] and peripheral blood film analysis). Discussion: Being the largest study on anaemia during pregnancy in a single geographical area in Sri Lanka, this study will provide important clues about geographical clustering of anaemia cases with similar etiology, associated factors and etiologies which would help to develop interventions to improve the health of pregnant women in the area. The possibility of selection bias is a potential limitation associated with the study design.

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Patterns OF Prevention OF Malaria in Pregnancy Among Pregnant Women at Selected Antenatal Care Facilities, Ilishan-Remo, Ogun State, Nigeria

African Journal of Health, Nursing and Midwifery ◽

10.52589/ajhnm-akih7uzw ◽

2021 ◽

Vol 4 (2) ◽

pp. 105-128

Author(s):

Ogungbesan O.F. ◽

Okafor N.A. ◽

Ogungbesan J.O.

Keyword(s):

Pregnant Women ◽

Tertiary Education ◽

Sampling Technique ◽

Malaria Prevention ◽

Cross Sectional Study ◽

Malaria In Pregnancy ◽

Chi Square ◽

Cross Sectional ◽

Ogun State ◽

In Pregnancy

Malaria in pregnancy is a preventable infectious disease, yet it remains the cause of maternal, in-utero morbidity and mortality. Pregnant women adopt different patterns of prevention against malaria occurrences and complications. This study aims to identify patterns of malaria prevention among pregnant women inIlishan Remo, Ogun State. In a descriptive cross-sectional study, a purposive sampling technique was used to proportionately select 271 pregnant women attending Ante-natal care (ANC) facilities. A self-administered questionnaire was used to obtained information on 56 items. Data were collected on clinic days for eight weeks duration and analyzed with SPSS 23 (for descriptive frequencies and percentages) and hypotheses tested with chi-square at p ≤ 0.005. One-third (35.4%) of the pregnant women aged 31-35years. Predominantly, 80.8% of the women were Christians, almost half (49.1%) had tertiary education. The mean of preventive measures used by pregnant women was 27.16±0.25. Slightly half (52.4%) always net windows and doors, 38.4% always and 53.4% often cleared bushes around house respectively. About 30.3% of pregnant women often used IPT-SP and 19.9% used direct observation therapy (DOT). On the categorized prevention patterns, half (54.2%) used conventional and 6.64% non-conventional. There was a significant relationship between the patterns of prevention and ANC facilities (χ2 =20.291), beliefs (χ2 = 16.540) at p< 0.005. The patterns of conventional and non-conventional prevention were used by pregnant women. However, pregnant women should be advised to use the recommended FMOH/WHO standard malaria prevention for safety.

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Antibiotic Prescriptions among China Ambulatory Care Visits of Pregnant Women: A Nationwide Cross-Sectional Study

Antibiotics ◽

10.3390/antibiotics10050601 ◽

2021 ◽

Vol 10 (5) ◽

pp. 601

Author(s):

Houyu Zhao ◽

Mei Zhang ◽

Jiaming Bian ◽

Siyan Zhan

Keyword(s):

Pregnant Women ◽

Ambulatory Care ◽

National Level ◽

Mainland China ◽

Antibiotic Use ◽

First Trimester ◽

Cross Sectional Study ◽

Cross Sectional ◽

Fetal Safety ◽

First Trimester Of Pregnancy

Background: Antibiotic use in pregnant women at the national level has rarely been reported in China. Objectives: We aimed to investigate antibiotic prescriptions during pregnancy in ambulatory care settings in China. Methods: Data of 4,574,961 ambulatory care visits of pregnant women from October 2014 to April 2018 were analyzed. Percentages of Antibiotic prescriptions by different subgroups and various diagnosis categories and proportions of inappropriate antibiotic prescriptions for different subgroups were estimated. Food and Drug Administration (FDA) pregnancy categories were used to describe the antibiotic prescription patterns. The 95% confidence intervals (CIs) were estimated using the Clopper––Pearson method or Goodman method. Results: Among the 4,574,961 outpatient visits during pregnancy, 2.0% (92,514 visits; 95% CI, 2.0–2.0%) were prescribed at least one antibiotic. The percentage of antibiotic prescriptions for pregnant women aged >40 years was 4.9% (95% CI, 4.7–5.0%), whereas that for pregnant women aged 26–30 years was 1.5% (95% CI, 1.4–1.5%). In addition, percentages of antibiotic prescriptions varied among different trimesters of pregnancy, which were 5.4% (95% CI, 5.3–5.4%) for the visits in the first trimester of pregnancy and 0.5% (95% CI, 0.4–0.5%) in the third trimester of pregnancy. Furthermore, the percentages of antibiotic prescriptions substantially varied among different diagnosis categories and nearly three-quarters of antibiotic prescriptions had no clear indications and thus might be inappropriate. In total, 130,308 individual antibiotics were prescribed; among these, 60.4% (95% CI, 60.0–60.8%) belonged to FDA category B, 2.7% (95% CI, 2.1–3.5%) were classified as FDA category D and 16.8% (95% CI, 16.2–17.4%) were not assigned any FDA pregnancy category. Conclusions: Antibiotic prescriptions in ambulatory care during pregnancy were not highly prevalent in mainland China. However, a substantial proportion of antibiotics might have been prescribed without adequate indications. Antibiotics whose fetal safety has not been sufficiently illustrated were widely used in pregnant women.

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Prevalence and Serotype Determination of Streptococcus agalactiae Isolated From Non-Pregnant Women in Tehran, Iran

ACTA MEDICA IRANICA ◽

10.18502/acta.v57i9.2641 ◽

2020 ◽

Author(s):

Leila Goudarzi ◽

Mohammad Bagher Khalili ◽

Mahmood Vakili ◽

Maryam Sadeh

Keyword(s):

Pregnant Women ◽

Streptococcus Agalactiae ◽

Group B Streptococcus ◽

Cross Sectional Study ◽

Chi Square ◽

Cross Sectional ◽

Specific Pcr ◽

Pcr Method ◽

Group B ◽

Tehran Area

Consequence of Streptococcus agalactiae, Group B Streptococcus (GBS) relating infant’s diseases are well documented. Although many women carry this bacterium in their vagina, they may transfer to their infant during delivery and may result in different neonatal invasive diseases. The aim of this study was to determine the prevalence of GBS and serotyping the isolated species among un-selective non-pregnant women who attended two gynecology clinics in Tehran. In this cross-sectional study, a total of 560 vaginal samples collected from non-pregnant women. Following inoculation of the specimen on Blood Agar, the standard technology was applied for the final identification of GBS. Detected GBS species were further confirmed using specific PCR directed on dlts gene. Capsular serotyping was done by using the multiplex PCR method. The chi-square method was used for statistical analysis. Fifty (8.9%) out of 560 non-pregnant women were carriers of GBS. The most common types were III (36%), followed by type II (32%), Ia (26%), and Ib (6%), respectively. Results represent that the prevalence rate of GBS in non-pregnant women was reliable and similar to what obtained from pregnant women. In addition, the serotype III was found the most dominant types, as well as other investigations in the Tehran area. Therefore, vaccine designation based on type III is recommended.

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Intimate partner violence against pregnant women: sociodemographic profile and characteristics of the aggressions

Revista Gaúcha de Enfermagem ◽

10.1590/1983-1447.2021.20200394 ◽

2021 ◽

Vol 42 ◽

Author(s):

Naiane Beatriz da Silva ◽

Rosely Erlach Goldman ◽

Hugo Fernandes

Keyword(s):

Black Women ◽

Pregnant Women ◽

Partner Violence ◽

Physical Violence ◽

First Trimester ◽

Cross Sectional Study ◽

Disease Notification ◽

Cross Sectional ◽

Notification System ◽

Sociodemographic Profile

ABSTRACT Objective: To identify the sociodemographic profile and the main characteristics of violence by intimate partners in pregnant women in São Paulo, Brazil. Method: Cross-sectional study based on notifications for suspected or confirmed cases of inter-police violence from the National Disease Notification System (SINAN) carried out in the 2016-2019 period. Collection was performed between March and June 2020. Chi-squared test or Fisher’s Exact test were used in statistical analysis. Results: A total of 4,269 notifications were obtained and the prevalent profile was women between 20 and 34 years old (62.5%), brown or black (51.3%), who have completed high school (22.5%) in the first trimester of pregnancy (44.2%). Physical violence was more frequent (48.3%), occurred at home (59.1%), motivated by sexism (22.29%). Sexual violence or rape was more frequent (85.4%) with abortion in cases provided for by law (39%). Conclusion: Adult brown or black women in the first gestational trimester experienced physical violence more frequently.

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Asymptomatic malaria and anemia among pregnant women during high and low malaria transmission seasons in Burkina Faso: household-based cross-sectional surveys in Burkina Faso, 2013–2017

10.21203/rs.3.rs-110522/v1 ◽

2020 ◽

Author(s):

Toussaint Rouamba ◽

Sékou Samadoulougou ◽

Mady Ouédraogo ◽

Hervé Hien ◽

Halidou Tinto ◽

...

Keyword(s):

Burkina Faso ◽

Pregnant Women ◽

Malaria Transmission ◽

Malaria In Pregnancy ◽

Asymptomatic Malaria ◽

Cross Sectional ◽

Transmission Season ◽

Asymptomatic Carriage ◽

Low Malaria Transmission ◽

In Pregnancy

Abstract Background: Malaria in endemic countries is often asymptomatic during pregnancy, but it has substantial consequences for both the mother and her unborn baby. In Burkina Faso, the prevalence and risk factors of asymptomatic malaria in pregnancy and its main consequence, anemia, during high and low malaria transmission seasons is underexplored at the household level.Methods: Data of 1751 pregnant women from October 2013 to March 2014 and 1931 pregnant women from April 2017 to June 2017 were drawn from two cross-sectional household surveys conducted in 24 health districts of Burkina Faso. Pregnant women were tested for malaria in their household after consenting. Asymptomatic carriage was defined as a positive result from malaria rapid diagnostic tests in the absence of malaria symptoms. Anemia was defined as hemoglobin level less than 11 g/dL.Results: Prevalence of asymptomatic malaria in pregnancy was estimated at 23.9% (95%CI: 20.2–28.0) during the high transmission season (October–November) in 2013. During the low transmission season, it was 12.7% (95%CI: 10.9–14.7) between December and March in 2013–2014 and halved (6.4%; 95%CI: 5.3–7.6) between April and June 2017. Anemia prevalence was estimated at 43.7% (95%CI: 39.3–48.3) during the high transmission season in 2013. During the low transmission season, it was 32.7% (95%CI: 30.1–35.5) between December and March 2013–2014 and 46.6% (95%CI: 44.3–48.9) between April and June, 2017.Conclusion: Prevalence of asymptomatic malaria in pregnancy was significantly higher during the high malaria transmission season while anemia prevalence was lower. Our study provides an opportunity to assess the prevalence of asymptomatic carriage and one of its main consequences, anemia, among pregnant women at the community level throughout the year. In order to mitigate the harmful effects of asymptomatic carriage for both the mother and fetus, health programs aimed at increasing the number of women coming into contact with health workers are necessary.

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