scholarly journals Genetic Uniformity of the MSXJ papaya hybrid (Carica papaya L.) during Micropropagation

2021 ◽  
Vol 14 (3) ◽  
Author(s):  
Dulce M. Ramírez-Hernández ◽  
Odón Castañeda-Castro ◽  
María Elena Galindo-Tovar ◽  
Luis A. Solano Rodríguez ◽  
Otto R. Leyva Ovalle ◽  
...  
Keyword(s):  
Carica Papaya ◽  
Quality Characteristics ◽  
Plant Tissues ◽  
Tropical Fruits ◽  
Commercial Success ◽  
Genetic Uniformity ◽  
In Vitro Plants ◽  
Ctab Method ◽  
Issr Primers

Objective: To analyze the genetic uniformity of MSXJ hybrid papaya in vitro plants, obtained by direct organogenesis.Design/Methodology/Approach: The MSXJ papaya hybrid demonstrates quality characteristics for the national and exports market. In vitro culture of plant tissues represents a useful tool for their multiplication and conservation, but somaclonal variation can diminish their genetic and agronomic uniformity. In order to analyze the genetic uniformity of in vitro plants of this hybrid, ten ISSR primers were used for in vitro plants micropropagated during nine subcultures. DNA was extracted using the CTAB method. Data were analyzed using the program PopGene v 1.3.1.Results: Eighty-five loci of 200 to up to 2000 pb were generated, with 37 polymorphic loci. In the cluster analysis, three groups were observed which separate subculture one, subcultures two to eight, and subculture nine; the Gst value of 0.87 indicated genetic uniformity as far as subculture eight.Study Limitations/Implications: Papaya is one of the most important tropical fruits worldwide; however, these plants need to be healthy and genetically uniform to guarantee commercial success. In vitro propagation allows obtaining healthy and uniform plants, but it is necessary to study genetic uniformity during their micropropagation.Findings/Conclusions: The in vitro multiplication of the MSXJ papaya hybrid permitted the regeneration of vigorous plants in 30 d. Molecular profiles indicate that as far as subculture eight, there is genetic uniformity. As such, no more thaneight subcultures are recommended during micropropagation.

scholarly journals Sex Identification of in vitro Plants of Carica papaya L. MSXJ Hybrid through Molecular Markers

2021 ◽  
Vol 14 (3) ◽  
Author(s):  
M. Nieto-Soriano ◽  
María E. Galindo-Tovar ◽  
Miriam C. Pastelín Solano ◽  
Luis A. Solano Rodríguez ◽  
Otto R. Leyva Ovalle ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Large Scale ◽  
Carica Papaya ◽  
Positive Control ◽  
Unwanted Sex ◽  
Running Water ◽  
In Vitro Plants ◽  
Hybrid Plants ◽  
Ctab Method

Objective: To identify the sex of in vitro plants of papaya (Carica papaya L.) MSXJhybrid obtained via somatic organogenesis, through SCAR type molecular markers. Design/Methodology/Approach: Eight-month old MSXJ papaya hybrid plants in thefructification stage were collected in Cotaxtla, Veracruz, Mexico. They weresuperficially disinfected with abundant running water, detergent during 30 min, andthen alcohol at 70% was added for one minute, commercial chlorine at 30% for 30min, and they were rinsed with sterile distilled water; then the meristems werecultivated in MS medium and after 30 d a subculture was made. The DNA extractionwas made with the CTAB method, and the DNA PCR was done with the Deputy et al.(2002) method, and the primers T1, T12 and W11 were used.Results: The T1 primer was the positive control and the T12 and W11 primersallowed the amplification of fragments that identify hermaphrodite, feminine and maleplants, while the T12 and W11 primers were specific for hermaphrodite plants.Study Limitations/Implications: It is required to standardize the method for it to beinexpensive.Findings/Conclusions: The sexuality of papaya plants can be differentiated until thestage of flowering, which is why the implementation of molecular markers wouldfacilitate plant selection if it is implemented at a large scale. Costs, maintenance timeand elimination of plants of unwanted sex are reduced this way.

scholarly journals Efecto de los factores determinantes para el establecimiento de microesquejes de papaya (Carica papaya L.)

Respuestas ◽  
2015 ◽  
Vol 15 (1) ◽  
pp. 5-12
Author(s):  
Jesús Arturo Ramírez Sulvarán
Keyword(s):  
Production Process ◽  
Key Words ◽  
Statistical Method ◽  
Carica Papaya ◽  
The Other ◽  
Tropical Fruits ◽  
Important Goal ◽  
Experimental Procedure ◽  
Micro Propagation

El objetivo fue determinar los efectos de los factores para el establecimiento de microesquejes de papaya. Se incluyeron los efectos de los factores: 1. Protocolo de desinfección. 2. Variedad. 3. Edad de la planta donadora. 4. Número de yemas por microesquejes. 5. Los componentes hormonales en los medios de cultivo y 6. La temperatura de desarrollo de los microesquejes. Los resultados se analizaron estadísticamente mediante tablas de contingencia. También se encontró la correlación entre las variables. Los resultados indican que las mejores condiciones para el establecimiento de los microesquejes fueron: 1.Desinfección con hipoclorito al 1,5% durante 5 minutos. 2. La variedad de papaya Roja Melonera. 3. La edad optima de las plantas donadora fue a los 60 días. 4. Los mejor microesquejes eran los que poseía 2 yemas. 5. La relación hormonal optima fue ANA 0,1 mg/l y BAP 0,5 mg/l. 6. La temperatura de desarrollo óptima fue a 27° C. Se concluyó que todos los factores estudiados presentaron efecto sobre el desarrollo y crecimiento de los microesquejes de papaya. De esta forma es necesario abordar estos procesos con un enfoque multifactorial.Palabras clave: Carica papaya, microesqueje, establecimiento, multifactorial. AbstractPrevious studies on apical and axilar microesqueje micropropagation of papaya have been lined up and without a systemic working. Consequently, it arises the actual research, which brough into study all the variables and parameters and their relationships, in order to improve the vitroplants production process, more efficiently. The main purpose of this study was to establish the effects of the former and in vitro factors, which have incidence upon the establishment of microesquejes. The effects of the former variables previous to the in vitro growing, which were studied, were: Sanitizing procedure, variety incidence, mother plant age incidence, among other ones. On the other hand, the in vitro variables studied were: temperature and hormonal compositions of the crops. Respuestas Año 15 No. 1 Junio 2010 ISSN 0122-820X This research was held at the vegetal biotechnology laboratory, which belongs to the biotechnology complex of the Universidad Francisco de Paula Santander in “Los Patios” Norte de Santander, Colombia. The experimental procedure was held with two months old plants from “Maradol” and “Roja Melonera” varieties. In order to accomplish the different effects of each one of the variables, it was used qualitative statistical method for their measurements. The final results showed that all factors were important to the papaya microesquejes micro propagation. As a result, the actual conclusion was to introduce the necessity to give a systemic focusing to the process in order to improve the efficiency in the vitroplants production, where all the factors and their relationships must be considered. Finally, the most important goal of this study is that all these results could be extended beyond other researches related to tropical fruits micro propagation, so important to the tropical micro propagation workers. Key Words: Carica papaya, microcuttings, establishment, multifactors.

scholarly journals USE OF IRAP MARKERS FOR CONTROL OF GENETIC STABILITY OF GISELA 6 ROOTSTOCKS IN VITRO AFTER CHEMOTHERAPY

2012 ◽  
Vol 14 ◽  
pp. 189-197
Author(s):  
N.V. Tryapitcyna ◽  
D.O. Kyselyov ◽  
T.V. Medvedyeva ◽  
R.O. Vasyliev
Keyword(s):  
Salicylic Acid ◽  
Genetic Stability ◽  
Genetic Uniformity ◽  
In Vitro Plants ◽  
Irap Markers ◽  
Gisela 6

Present study covers the use of IRAP markers for identification of Gisela-6 rootstocks and detection of genetic uniformity of in vitro plants treated with different concentrations of Amixin® ІС and SA (salicylic acid) for chemotherapeutic purpose.

Performance of hermaphrodite Carica papaya in-vitro plants grown under greenhouse conditions in the tropics

2019 ◽  
pp. 159-164
Author(s):  
F.L. Espadas-Gil ◽  
J. Morales-Landa ◽  
C.R. Talavera-May ◽  
J. Patrón Castro ◽  
F. Cámara ◽  
...  
Keyword(s):  
Carica Papaya ◽  
In Vitro Plants ◽  
The Tropics

The role of silicon in forages: A quantitative X-Ray study

1987 ◽  
Vol 45 ◽  
pp. 416-417
Author(s):  
Janet H. Woodward ◽  
D. E. Akin
Keyword(s):  
Sodium Acetate ◽  
Dry Matter ◽  
Acetate Buffer ◽  
Plant Tissues ◽  
Sodium Acetate Buffer ◽  
X Ray ◽  
Dry Matter Digestibility ◽  
Percent Composition

Silicon (Si) is distributed throughout plant tissues, but its role in forages has not been clarified. Although Si has been suggested as an antiquality factor which limits the digestibility of structural carbohydrates, other research indicates that its presence in plants does not affect digestibility. We employed x-ray microanalysis to evaluate Si as an antiquality factor at specific sites of two cultivars of bermuda grass (Cynodon dactvlon (L.) Pers.). “Coastal” and “Tifton-78” were chosen for this study because previous work in our lab has shown that, although these two grasses are similar ultrastructurally, they differ in in vitro dry matter digestibility and in percent composition of Si.Two millimeter leaf sections of Tifton-7 8 (Tift-7 8) and Coastal (CBG) were incubated for 72 hr in 2.5% (w/v) cellulase in 0.05 M sodium acetate buffer, pH 5.0. For controls, sections were incubated in the sodium acetate buffer or were not treated.

Synergistic in-vitro anthelmintic potentials of Vernonia amygdalina Delile stem and Carica papaya Lin. seeds

2017 ◽  
Author(s):  
A Quartey ◽  
A Oppong ◽  
I Ayensu ◽  
J Apenteng ◽  
D Mintah ◽  
...  
Keyword(s):  
Carica Papaya ◽  
Vernonia Amygdalina

Influence of salicylic acid on organogenesis of in vitro plants Fragaria ananassa Duch.

2016 ◽  
Vol 48 (1) ◽  
pp. 26-33
Author(s):  
O.V. Subin ◽  
◽  
M.D. Melnychuk ◽  
A.F. Likhanov ◽  
O.L. Klyachenko ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Fragaria Ananassa ◽  
In Vitro Plants

Phytochemical analysis and salivary amylase inhibition activities of Carica papaya leaf and Garcinia mangostana pericarp extracts and partially purified fractions

2017 ◽  
Vol 6 (1) ◽  
pp. 34
Author(s):  
Michael Russelle Alvarez ◽  
Paolo Robert Bueno ◽  
Raymond Oliver Cruz ◽  
Richard Macapulay ◽  
Francis Jayson Vallesfin ◽  
...  
Keyword(s):  
Crude Extract ◽  
Carica Papaya ◽  
Uv Light ◽  
Digestive Enzyme ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Salivary Amylase ◽  
Garcinia Mangostana ◽  
Leaf Extracts

Plant-derived digestive enzyme inhibitors particularly those targeted to carbohydrate metabolism has been the focus of recent studies as natural supplements for weight control and diabetes. The present study explores the salivary amylase inhibition activity of Garcinia mangostana (Linn.) pericarp extracts and Carica papaya (Linn.) leaf extracts and fractions, as well as perform phytochemical screening and quantification, and thin layer – and high performance liquid chromatographic profiling. ­Results show that crude extracts and purified fractions were able to inhibit salivary amylase, with C. papaya fraction 1 being the most active at 30.89% inhibition. Phytochemical screening of all extracts tested ­positive for tannins, glycosides, phenolics, flavonoids and alkaloids. Quantification of phenolics showed that extracts contained high levels of phenolics, with C. papaya crude extract having the highest content with 219.0±12.7 mg GAE/g extract followed by G. mangostana crude extract with 247.1±18.0 mg GAE/g extract. Quantification of total flavonoids also showed C. papaya crude extract to contain the highest content with 55.12±0.679 mg QE/g extract. All extracts contained negligible alkaloid content, though. HPLC and TLC profiling showed several peaks and bands, when viewed in 210 nm and UV light, respectively. These results demonstrate in vitro the salivary amylase inhibitory activity of both plants and their potential as antidiabetic drug candidates; however, further studies need to be done, like isolation and structure elucidation of active components and toxicity assays. Keywords: Amylase inhibition, phytochemical quantification, Carica papaya, Garcinia mangostana

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