scholarly journals Efecto de los factores determinantes para el establecimiento de microesquejes de papaya (Carica papaya L.)

Respuestas ◽  
2015 ◽  
Vol 15 (1) ◽  
pp. 5-12
Author(s):  
Jesús Arturo Ramírez Sulvarán
Keyword(s):  
Production Process ◽  
Key Words ◽  
Statistical Method ◽  
Carica Papaya ◽  
The Other ◽  
Tropical Fruits ◽  
Important Goal ◽  
Experimental Procedure ◽  
Micro Propagation

El objetivo fue determinar los efectos de los factores para el establecimiento de microesquejes de papaya. Se incluyeron los efectos de los factores: 1. Protocolo de desinfección. 2. Variedad. 3. Edad de la planta donadora. 4. Número de yemas por microesquejes. 5. Los componentes hormonales en los medios de cultivo y 6. La temperatura de desarrollo de los microesquejes. Los resultados se analizaron estadísticamente mediante tablas de contingencia. También se encontró la correlación entre las variables. Los resultados indican que las mejores condiciones para el establecimiento de los microesquejes fueron: 1.Desinfección con hipoclorito al 1,5% durante 5 minutos. 2. La variedad de papaya Roja Melonera. 3. La edad optima de las plantas donadora fue a los 60 días. 4. Los mejor microesquejes eran los que poseía 2 yemas. 5. La relación hormonal optima fue ANA 0,1 mg/l y BAP 0,5 mg/l. 6. La temperatura de desarrollo óptima fue a 27° C. Se concluyó que todos los factores estudiados presentaron efecto sobre el desarrollo y crecimiento de los microesquejes de papaya. De esta forma es necesario abordar estos procesos con un enfoque multifactorial.Palabras clave: Carica papaya, microesqueje, establecimiento, multifactorial. AbstractPrevious studies on apical and axilar microesqueje micropropagation of papaya have been lined up and without a systemic working. Consequently, it arises the actual research, which brough into study all the variables and parameters and their relationships, in order to improve the vitroplants production process, more efficiently. The main purpose of this study was to establish the effects of the former and in vitro factors, which have incidence upon the establishment of microesquejes. The effects of the former variables previous to the in vitro growing, which were studied, were: Sanitizing procedure, variety incidence, mother plant age incidence, among other ones. On the other hand, the in vitro variables studied were: temperature and hormonal compositions of the crops. Respuestas Año 15 No. 1 Junio 2010 ISSN 0122-820X This research was held at the vegetal biotechnology laboratory, which belongs to the biotechnology complex of the Universidad Francisco de Paula Santander in “Los Patios” Norte de Santander, Colombia. The experimental procedure was held with two months old plants from “Maradol” and “Roja Melonera” varieties. In order to accomplish the different effects of each one of the variables, it was used qualitative statistical method for their measurements. The final results showed that all factors were important to the papaya microesquejes micro propagation. As a result, the actual conclusion was to introduce the necessity to give a systemic focusing to the process in order to improve the efficiency in the vitroplants production, where all the factors and their relationships must be considered. Finally, the most important goal of this study is that all these results could be extended beyond other researches related to tropical fruits micro propagation, so important to the tropical micro propagation workers. Key Words: Carica papaya, microcuttings, establishment, multifactors.

scholarly journals Karakterisasi Tingkat Produksi Duku Berbasis Pewilayahan Hujan di Provinsi Jambi

2016 ◽  
Vol 7 (2) ◽  
pp. 121
Author(s):  
Fendy Arifianto ◽  
Yonny Koesmaryono ◽  
Impron .
Keyword(s):  
Key Words ◽  
Economic Value ◽  
Provincial Government ◽  
Land Suitability ◽  
The Other ◽  
Rainfall Pattern ◽  
Tropical Fruits ◽  
Precipitation Characteristics ◽  
Government Work ◽  
Lansium Domesticum

<p align="center"><strong><em>ABSTRACT</em></strong></p><p><em>Duku </em><em>(<span style="text-decoration: underline;">Lansium</span> <span style="text-decoration: underline;">domesticum</span> Corr) </em><em>is one of tropical fruits </em><em>and of</em><em> high economic value. The Jambi provincial government work</em><em>s</em><em> to maintain and develop duku </em><em>production</em><em> through improv</em><em>ement</em><em> of cultivation and expansion. </em><em>T</em><em>he supporting factor for success of duku production was the weather especially precipitation. This study was conducted to </em><em>obtain precipitation characteristics</em><em> </em><em>on land suitability</em><em> </em><em>of duku productivity </em><em>in Jambi Province. The results showed that the rainfall pattern in Jambi province had </em><em>five</em><em> characters</em><em> </em><em>in which the annual precipitation 2583 kg tree<sup>-1</sup>yr<sup>-1</sup></em><em>. The average of duku producti</em><em>vity</em><em> in </em><em>rainfall pattern I dan II</em><em> was</em><em> </em><em>269 </em><em>kg tree<sup>-1</sup>yr<sup>-1</sup>, and the other result outside rinfall pattern I and II had the average productivity 370 kg tree<sup>-1 </sup>yr<sup>-1</sup>.</em><em> </em></p><p><em>Key words: duku, Jambi Province,land suitability, precipitation, productivity</em></p><p><strong> </strong></p><p align="center"><strong>ABSTRAK <br /></strong></p><p>Duku (<em>Lansium domesticum </em>Corr) merupakan salah satu buah tropis yang memiliki nilai jual yang cukup tinggi. Pemerintah Daerah Provinsi Jambi berupaya untuk mempertahankan dan mengembangkan produksi duku melalui perbaikan budidaya tanaman dan perluasan lahan. Faktor yang menjadi pendukung terhadap keberhasilan produksi suatu tanaman adalah faktor iklim terutama curah hujan dan suhu. Penelitian ini bertujuan untuk mendapatkan karakteristik curah hujan wilayah untuk kesesuaian lahan tanaman duku di Provinsi Jambi. Hasil penelitian menunjukkan bahwa pola curah hujan wilayah di Provinsi Jambi memiliki lima pola dengan curah hujan rata-rata tahunan sebesar 2583 mm tahun<sup>-1</sup>. Rata-rata produksi duku di wilayah yang berpola hujan I dan II 269 kg pohon<sup>-1 </sup>tahun<sup>-1</sup>, sedangkan daerah yang diluar pola tersebut memiliki rata-rata produktivitas sebesar 370 kg pohon<sup>-1</sup>tahun<sup>-1</sup>.</p><p>Kata kunci: duku, kesesuaian lahan, presipitasi, produktivitas, Provinsi Jambi</p>

scholarly journals In-Vitro androgenesis in papaya (Carica papaya L.) cv. Pusa Nanha

2015 ◽  
Vol 7 (1) ◽  
pp. 273-278 ◽  
Author(s):  
Gyanchand Gyanchand ◽  
Manoj Kumar Sharma ◽  
Sandeep Kumar ◽  
Sushma Sagar ◽  
Vinay Kumar ◽  
...  
Keyword(s):  
Anther Culture ◽  
Carica Papaya ◽  
Industrial Applications ◽  
Liquid Form ◽  
Induction Rate ◽  
In Vitro Androgenesis ◽  
Embryo Induction ◽  
Micro Propagation ◽  
The Media

Papaya (Carica papaya L.) is an economically important fruit crop of tropics and subtropics. It has high nutritional value, as well as medicinal and industrial applications. Papaya is a polygamous species with three sex types male, female, and hermaphrodite. Conventional methods of papaya breeding are time consuming and needs advent of anther culture which may be effective for shortening of breeding cycles. The present study on in vitro androgenesis in papaya cv. Pusa Nanha observed the highest embryo induction rate (8.0%) when anthers werecultured on agar medium with 0.1 mg/L BA and 0.1 mg/L NAA after incubation in liquid MS medium with 2.0% sucrose for 7 days at 35ºC. The high temperature (35ºC) was more suitable for embryo induction in papaya than slightly low temperature (25ºC). At these both temperatures longer incubation of anthers in water reduced embryo induction rate. Sugar starvation results were ambiguous. Shoots were also developed in the media when used in liquid form. The highest rooting (75.0%) was observed at 2.0 mg/L IBA. Increasing IBA concentration reduced rooting. All well rooted plants were hardened in hardening chamber and successfully transferred to field. The present findings indicated that anther culture can be efficiently contributed for the direct micro-propagation of papaya plants. This study would also be helpful to the researchers to develop more efficient anther culture protocols for further improvement of papaya through in vitro androgenesis.

The effect of the natural assemblage of microorganisms and selected strains of the yeast Metschnikowia reukaufii in controlling the germination of pollen of the common milkweed Asclepias syriaca

1990 ◽  
Vol 68 (5) ◽  
pp. 1163-1165 ◽  
Author(s):  
D. Eisikowitch ◽  
M. A. Lachance ◽  
P. G. Kevan ◽  
S. Willis ◽  
D. L. Collins-Thompson
Keyword(s):  
Key Words ◽  
Pollen Germination ◽  
Natural Habitat ◽  
Pollen Tubes ◽  
The Other ◽  
Asclepias Syriaca ◽  
Natural Assemblage ◽  
The Common ◽  
Germination Of Pollen

The yeast Metschnikowia reukaufii is a natural contaminant of nectar and is vectored to the flowers of the field milkweed Asclepias syriaca by insects, some of which are pollinators of the plants. In its natural habitat, the yeast inhibits the germination of the milkweed's pollen, which normally uses nectar in the stigmatic cavity for germination. This inhibition is irreversible after about 8 h of exposure to the yeasts. Two selected strains of the yeast were isolated and investigated for their effects on pollen germination in vitro. The two strains, and their mixture, affected pollen germination adversely by reducing its amount and vigour and causing any pollen tubes that were produced to burst: One strain was more virulent than the other, and the mixture seemed to have an additive effect. The strains may be more efficacious than the natural assemblage of microbes in disrupting fertilization of milkweed flowers because they cause the immediate death (bursting) of the growing microgametophyte (pollen and tube). Key words: yeasts, pollen germination, milkweed, Metschnikowia reukaufii, Asclepias syriaca.

Genotypic differences in chemical composition and ruminal degradability of oat hulls

2000 ◽  
Vol 80 (2) ◽  
pp. 377-379 ◽  
Author(s):  
R. K. Thompson ◽  
A. F. Mustafa ◽  
J. J. McKinnon ◽  
D. Maenz ◽  
B. Rossnagel
Keyword(s):  
Chemical Composition ◽  
Key Words ◽  
Dry Matter ◽  
The Other ◽  
Genotypic Differences ◽  
Oat Hulls ◽  
Acid Detergent Lignin ◽  
Ruminal Degradability

A study was conducted to determine differences in chemical composition and ruminal degradability of oat hulls derived from ten oat varieties. Hulls derived from AC Assiniboia had a lower (P < 0.05) acid detergent lignin (ADL) and a higher (P < 0.05) in vitro dry matter disappearance (IVDMD) relative to the other nine varieties. Effective ruminal degradability of neutral (NDF) and acid (ADF) detergent fiber for AC Assiniboia hulls were higher (P < 0.05) than those of Calibre hulls and similar to those of Calibre straw. Key words: Oat hulls, chemical composition, ruminal degradability

scholarly journals Genetic Uniformity of the MSXJ papaya hybrid (Carica papaya L.) during Micropropagation

2021 ◽  
Vol 14 (3) ◽  
Author(s):  
Dulce M. Ramírez-Hernández ◽  
Odón Castañeda-Castro ◽  
María Elena Galindo-Tovar ◽  
Luis A. Solano Rodríguez ◽  
Otto R. Leyva Ovalle ◽  
...  
Keyword(s):  
Carica Papaya ◽  
Quality Characteristics ◽  
Plant Tissues ◽  
Tropical Fruits ◽  
Commercial Success ◽  
Genetic Uniformity ◽  
In Vitro Plants ◽  
Ctab Method ◽  
Issr Primers

Objective: To analyze the genetic uniformity of MSXJ hybrid papaya in vitro plants, obtained by direct organogenesis.Design/Methodology/Approach: The MSXJ papaya hybrid demonstrates quality characteristics for the national and exports market. In vitro culture of plant tissues represents a useful tool for their multiplication and conservation, but somaclonal variation can diminish their genetic and agronomic uniformity. In order to analyze the genetic uniformity of in vitro plants of this hybrid, ten ISSR primers were used for in vitro plants micropropagated during nine subcultures. DNA was extracted using the CTAB method. Data were analyzed using the program PopGene v 1.3.1.Results: Eighty-five loci of 200 to up to 2000 pb were generated, with 37 polymorphic loci. In the cluster analysis, three groups were observed which separate subculture one, subcultures two to eight, and subculture nine; the Gst value of 0.87 indicated genetic uniformity as far as subculture eight.Study Limitations/Implications: Papaya is one of the most important tropical fruits worldwide; however, these plants need to be healthy and genetically uniform to guarantee commercial success. In vitro propagation allows obtaining healthy and uniform plants, but it is necessary to study genetic uniformity during their micropropagation.Findings/Conclusions: The in vitro multiplication of the MSXJ papaya hybrid permitted the regeneration of vigorous plants in 30 d. Molecular profiles indicate that as far as subculture eight, there is genetic uniformity. As such, no more thaneight subcultures are recommended during micropropagation.

scholarly journals Effect of Relative Humidity on the Development of Head Blight by Bipolaris sorokiniana in Wheat

1970 ◽  
Vol 4 (1) ◽  
pp. 61-65 ◽  
Author(s):  
Sheikh Afsar Uddin ◽  
KM Khalequzzaman ◽  
AQM Bazlur Rashid
Keyword(s):  
Relative Humidity ◽  
Key Words ◽  
Grain Quality ◽  
Control Variable ◽  
Bipolaris Sorokiniana ◽  
Leaf Blight ◽  
The Other ◽  
High Humidity ◽  
Head Blight

Effect of exposure to different duration of high humidity (5,10,15,20,25 & 30 days) on the development of head blight caused by Bipolaris sorokiniana in wheat was studied in vitro. Maximum leaf blight severity of 42.97% and head blight development of 87.53% caused by the pathogen were recorded for the infected plants incubated for a period of 30 days under condition of high humidity. The treatments did not differ significantly with respect to head blight development except the control. Variable effects of the treatments was observed on the grain quality. The highest diseased (black pointed and shriveled) grains of 62.27% were recorded in treatment 6 (30 days exposure) followed by treatment 5 (25 days exposure) with 48.01% diseased grains. There were no significant differences among the other treatments except the check. Key words: Humidity, head blight, leaf blight, Bipolaris sorokiniana, wheat.

Synergistic in-vitro anthelmintic potentials of Vernonia amygdalina Delile stem and Carica papaya Lin. seeds

2017 ◽  
Author(s):  
A Quartey ◽  
A Oppong ◽  
I Ayensu ◽  
J Apenteng ◽  
D Mintah ◽  
...  
Keyword(s):  
Carica Papaya ◽  
Vernonia Amygdalina

Enhancement of ADP-induced Platelet Aggregation by Adrenaline in Vivo and Its Prevention

1973 ◽  
Vol 29 (02) ◽  
pp. 490-498 ◽  
Author(s):  
Hiroh Yamazaki ◽  
Itsuro Kobayashi ◽  
Tadahiro Sano ◽  
Takio Shimamoto
Keyword(s):  
Platelet Count ◽  
Platelet Aggregation ◽  
Platelet Rich Plasma ◽  
The Other ◽  
Endothelial Surface ◽  
Important Interaction ◽  
Blood Coagulability ◽  
The Difference

SummaryThe authors previously reported a transient decrease in adhesive platelet count and an enhancement of blood coagulability after administration of a small amount of adrenaline (0.1-1 µg per Kg, i. v.) in man and rabbit. In such circumstances, the sensitivity of platelets to aggregation induced by ADP was studied by an optical density method. Five minutes after i. v. injection of 1 µg per Kg of adrenaline in 10 rabbits, intensity of platelet aggregation increased to 115.1 ± 4.9% (mean ± S. E.) by 10∼5 molar, 121.8 ± 7.8% by 3 × 10-6 molar and 129.4 ± 12.8% of the value before the injection by 10”6 molar ADP. The difference was statistically significant (P<0.01-0.05). The above change was not observed in each group of rabbits injected with saline, 1 µg per Kg of 1-noradrenaline or 0.1 and 10 µg per Kg of adrenaline. Also, it was prevented by oral administration of 10 mg per Kg of phenoxybenzamine or propranolol or aspirin or pyridinolcarbamate 3 hours before the challenge. On the other hand, the enhancement of ADP-induced platelet aggregation was not observed in vitro, when 10-5 or 3 × 10-6 molar and 129.4 ± 12.8% of the value before 10∼6 molar ADP was added to citrated platelet rich plasma (CPRP) of rabbit after incubation at 37°C for 30 second with 0.01, 0.1, 1, 10 or 100 µg per ml of adrenaline or noradrenaline. These results suggest an important interaction between endothelial surface and platelets in connection with the enhancement of ADP-induced platelet aggregation by adrenaline in vivo.

MICROPROPAGATION OF CHICKPEA FROM SHOOT TIP AND NODE SEGMENT

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
J. D. BARSHILE
Keyword(s):  
Shoot Tip ◽  
Root Induction ◽  
Multiple Response ◽  
Ms Medium ◽  
Growth Responses ◽  
Rapid Multiplication ◽  
Micro Propagation ◽  
G 12 ◽  
Better Than

Present investigation was undertaken to standardize technique for in vitro micro-propagation of chickpea( Cicer arietinum ) cultivar Vishwas (Phule G 12). Micropropagation method for chickpea was established and this method enabled much more efficient propagation of plants. The present work was aimed at evolving a protocol for rapid multiplication of chickpea using micropropagation technique. Explants from shoot tip and node segment were cultured on MS medium supplemented with different concentrations of BAP and Kinetin (1.0 to 2.5 mg/l) and their growth responses like shooting were elucidated. The maximum multiple response was observed with 2 mg/l concentration of BAP from both types of explant. The highest number of shoots (12.5 ± 0.3) was achieved on MS medium with 2 mg/l BAP using node segments. The medium supplemented with 2 mg/l of BAP was found better than all other concentrations. Individual shoots were transferred to IBA and IAA (1.0-1.5 mg/l) for root induction. MS medium supplemented with 2 mg/l of IBA proved better for rooting. Rooted plantlets were successfully hardened in greenhouse and established in the pot.

Sign in / Sign up

Export Citation Format

Share Document