scholarly journals Reproductive characterization of the female Mexican turkey (Meleagris gallopavo L.)

2021 ◽  
Author(s):  
Elvia López-Pérez ◽  
Jaime Gallegos-Sánchez ◽  
Arturo Pró Martínez ◽  
Juan M. Cuca-García ◽  
Juan A. Herrera-Barragán
Keyword(s):  
Assisted Reproduction ◽  
Artificial Insemination ◽  
Rural Areas ◽  
Genetic Material ◽  
Meleagris Gallopavo ◽  
Reproductive Activity ◽  
First Year ◽  
Luteinizing Hormones ◽  
Reproductive Characteristics ◽  
Practical Experiences

Objective: To describe the anatomy, morphology, physiology, and reproductive characteristics of female turkeys. Design/methodology/approach: An analysis of the practical experiences of 10 years with Mexican native turkeys was conducted and complemented with scientific literature. We focused on reproductive aspects, anatomy, physiology and assisted reproduction. Results: Information on the breeding of Mexican native turkeys is scarce, few families in rural areas are dedicated to raising this poultry. Therefore, empirical knowledge on their management is being lost. The female turkey begins oviposition at 6 months of age with a body weight between 2 and 4 kg. The structures that make up their reproductive system are the ovary, developing follicles, left oviduct, and cloaca. The reproductive activity is initiated by photo receptors located in the retina and pineal gland, triggering follicle stimulating and luteinizing hormones release. Egg formation lasts from 24 to 26 hours. During the first year, females lay between 36 to 54 eggs in clutches of 12 to 18 eggs and in the second year from 30 to 45 eggs. Chloequez is prolactin induced. As for assisted reproduction, artificial insemination techniques in female turkeys are uncommon. Limitations on study/implications: Few rural families raise turkeys and since they lack economic resources, reproductive strategies are not used to improve their production. Findings/conclusions: The anatomical, morphological, physiological and reproductive characteristics of the native female turkeys from Mexico were documented. In native turkeys, artificial insemination techniques are not of common use, but their implementation may be adequate to conserve genetic material from animals with outstanding characteristics.

scholarly journals The native turkey (Meleagris gallopavo): male morphophysiology

2021 ◽  
Author(s):  
Elvia López-Pérez ◽  
Jaime Gallegos-Sánchez ◽  
Arturo Pró-Martínez ◽  
Juan M. Cuca-García ◽  
Francisco Calderón-Sánchez ◽  
...  
Keyword(s):  
Genetic Resources ◽  
Breeding Season ◽  
Reproductive Performance ◽  
Reproductive Strategies ◽  
Production Management ◽  
Meleagris Gallopavo ◽  
Low Resource ◽  
Reproductive Characteristics ◽  
Testicular Growth ◽  
Practical Experiences

Objective: To describe the morphophysiology and reproductive characteristics of the male turkey. Methodology: An analysis based on the practical experiences from over 10 years with the native Mexican turkey was developed and complemented with literature reports. Results: In Mexico the production management is customs based on traditions and beliefs. In their reproductive morphophysiology, the epididymis absence stands out, as well as a rapid testicular growth during the breeding season, which is controlled by the photoperiod. Limitations of the study: Native turkey farming is practiced in backyard, in marginalized and low-resource areas that have no strategies (nutritional and reproductive) to improve their production. Conclusions: The anatomical, morphological, physiological and reproductive characteristics of the native Mexican turkey were documented. The knowledge on these characteristics will allow to develop feeding and reproductive strategies to improve the productive and reproductive performance of the native turkey and preserve their genetic resources.

scholarly journals REPRODUCTIVE ASPECTS OF JAGUAR (PANTHERA ONCA): FEMALE

2021 ◽  
Author(s):  
E. López-Pérez ◽  
F. Cortés-Villavicencio ◽  
A. Ávalos-Rodríguez ◽  
J. Gallegos-Sánchez
Keyword(s):  
Literature Review ◽  
Assisted Reproduction ◽  
Reproductive Strategies ◽  
Design Methodology ◽  
Genetic Material ◽  
Free Living ◽  
Assisted Reproduction Techniques ◽  
Reproductive Characteristics ◽  
Reproduction Strategy ◽  
Documented Information

Objective: Describe the reproductive characteristics of the female jaguar as well as assisted reproduction techniques. Design/methodology/approach: A literature review about reproductive characteristics and assisted reproduction techniques was done to documented information about it. Results: The Jaguar is considered the largest feline in America, females are 1.57 to 2.19 m of length and weigh 45 to 82 kg. The jaguar female is ready for mating when it is about 2 years old. Regarding the estrous cycle, it is divided into: proestrus, estrus, diestrous, interestrous and anestrus. Environment influence on the reproductive behavior of the jaguar, being the steroids those that stimulate the production of pheromones to attract the male; in the female the secretion is cyclical. The gestation lasts approximately between 90 and 110 days and they usually give birth to 1 or up to 4 young. In the case of assisted reproduction, artificial insemination could be useful to preserve genetic material from animals with outstanding characteristics. Limitations on study/implications: The destruction of forests and jungles, the fragmentation of habitat, indiscriminate hunting, the lack of prey as a food source and the conflict with ranchers has put the Jaguar on the brink of extinction, in addition there is little information about its reproductive characteristics, which is why in Mexico, it is imperative to generate this information, mainly in free-living jaguar. Findings/conclusions: Reproductive characteristics of jaguar female were documented from a literature review as well as assisted reproduction techniques. Knowledge of these will allow developed reproductive strategies to work on a national conservation and reproduction strategy to preserve the species.

scholarly journals Sperm Cryopreservation in American Flamingo (Phoenicopterus Ruber): Influence of Cryoprotectants and Seminal Plasma Removal

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 203
Author(s):  
María Gemma Millán de la Blanca ◽  
Eva Martínez-Nevado ◽  
Cristina Castaño ◽  
Juncal García ◽  
Berenice Bernal ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Genetic Material ◽  
First Year ◽  
Sperm Cryopreservation ◽  
Straight Line ◽  
The Family ◽  
Second Year ◽  
American Flamingo ◽  
Phoenicopterus Ruber

The American flamingo is a useful model for the development of successful semen cryopreservation procedures to be applied to threatened related species from the family Phoenicopteridae, and to permit genetic material banking. Current study sought to develop effective sperm cryopreservation protocols through examining the influences of two permeating cryoprotectants and the seminal plasma removal. During two consecutive years (April), semen samples were collected and frozen from American flamingos. In the first year, the effect of two permeating cryoprotectants, DMA (dimethylacetamide) (6%) or Me2SO (dimethylsulphoxide) (8%), on frozen–thawed sperm variables were compared in 21 males. No differences were seen between DMA and Me2SO for sperm motility, sperm viability, and DNA fragmentation after thawing. In the second year, the role of seminal plasma on sperm cryoresistance was investigated in 31 flamingos. Sperm samples were cryopreserved with and without seminal plasma, using Me2SO (8%) as a cryoprotectant. The results showed that samples with seminal plasma had higher values than samples without seminal plasma for the following sperm variables: Straight line velocity (22.40 µm/s vs. 16.64 µm/s), wobble (75.83% vs. 69.40%), (p < 0.05), linearity (62.73% vs. 52.01%) and straightness (82.38% vs. 73.79%) (p < 0.01); but acrosome integrity was lower (55.56% vs. 66.88%) (p < 0.05). The cryoresistance ratio (CR) was greater in samples frozen with seminal plasma than without seminal plasma for CR-progressive motility (138.72 vs. 54.59), CR-curvilinear velocity (105.98 vs. 89.32), CR-straight line velocity (152.77 vs. 112.58), CR-average path velocity (122.48 vs. 98.12), CR-wobble (111.75 vs. 102.04) (p < 0.05), CR-linearity (139.41 vs. 113.18), and CR-straightness (124.02 vs. 109.97) (p < 0.01). This research demonstrated that there were not differences between Me2SO and DMA to successful freezing sperm of flamingos; seminal plasma removal did not provide a benefit for sperm cryopreservation.

scholarly journals Impact of “non-lethal” tarsal clipping on bumble bees (Bombus vosnesenskii) may depend on queen stage and worker size

2021 ◽  
Author(s):  
John M. Mola ◽  
Clara Stuligross ◽  
Maureen L. Page ◽  
Danielle Rutkowski ◽  
Neal M. Williams
Keyword(s):  
Genetic Material ◽  
Population Monitoring ◽  
First Year ◽  
Negative Effects ◽  
Worker Size ◽  
Monitoring Tools ◽  
Individual Survival ◽  
Body Sizes ◽  
Negative Effect ◽  
Worker Caste

Abstract Recent bumble bee declines have prompted the development of novel population monitoring tools, including the use of putatively non-lethal tarsal clipping to obtain genetic material. However, the potential side effects of tarsal clipping have only been tested in the worker caste of a single domesticated species, prompting the need to more broadly test whether tarsal clipping negatively affects sampled individuals. To determine if tarsal clipping reduces queen survivorship and colony establishment, we collected wild queens of Bombus vosnesenskii and clipped tarsi from a single leg of half the individuals. We reared captive queens and estimated survivorship and nest establishment success. We also clipped tarsi of workers from a subset of colonies across a range of body sizes. We found no consistent negative effect of clipping on queen survival. In the first year, clipped nest-searching queens suffered heavy mortality, but there was no effect on foraging queens. The following year, we found no effect of clipping on queen survival or establishment. Clipping did not reduce overall worker survival but reduced survivorship for those in the smallest size quartile. Implications for insect conservation Our findings suggest tarsal clipping does not have consistent negative effects on individual survival. However, our results varied with queen behavioral state, year, and worker size, suggesting differences within and among species and interactions with landscape stressors warrant further study. In the interim, we recommend researchers and conservationists minimize the use of tarsal clipping for sensitive species, populations, or small workers except in cases of exceptional scientific need.

scholarly journals Identification of Leishmania spp. in horses and a dog from rural areas of Uruguaiana, Rio Grande do Sul, Brazil

2020 ◽  
Vol 41 (6) ◽  
pp. 2687-2694
Author(s):  
Gabriela Döwich Pradella ◽  
◽  
Taiane Acunha Escobar ◽  
Claudia Acosta Duarte ◽  
Irina Lübeck ◽  
...  
Keyword(s):  
Rural Areas ◽  
Direct Sequencing ◽  
Geographical Area ◽  
Genetic Material ◽  
Rio Grande ◽  
Sand Fly ◽  
Rio Grande Do Sul ◽  
Transmission Area ◽  
Leishmania Spp ◽  
Polymerase Chain

Visceral leishmaniasis (VL) is a zoonosis caused by the protozoan of the genus Leishmania. The disease is transmitted by the bite of a sand fly vector. Although the main reservoirs are dogs, other hosts can be infected and may play this role. Rio Grande do Sul western region, located on the triple border of Brazil-Uruguay-Argentina, represents a VL transmission area. The goal of the present study was to identify Leishmania spp. infection in animals from rural areas of Uruguaiana, Rio Grande do Sul. Nine farms in the Uruguaiana municipality, Rio Grande do Sul state, were included. Peripheral blood samples were collected from 113 animals (canine [n=22], equine [n=91]) for detection of Leishmania spp. DNA was isolated and polymerase chain reaction was performed. Eight (7%) animals with Leishmania spp. infection were detected on two farms in the same geographical area, seven of which were horses and one was canine, all of which were asymptomatic. To investigate the species of Leishmania, one of the positive equine samples was subjected to direct sequencing, which confirmed the presence of L. infantum genetic material. Results of this study confirm the presence of L. infantum-infected animals in rural areas of Uruguaiana, and provide evidence supporting further investigation of risk factors for dissemination in such areas.

scholarly journals Reproductive aspects of the male jaguar (Panthera onca): A review

2021 ◽  
Author(s):  
E. López-Pérez ◽  
F. Cortés-Villavicencio ◽  
C. Muñoz-García ◽  
J. Gallegos-Sánchez ◽  
Alejandro Ávalos-Rodríguez
Keyword(s):  
In Vitro Fertilization ◽  
Assisted Reproduction ◽  
Reproductive System ◽  
Artificial Insemination ◽  
Relevant Information ◽  
Panthera Onca ◽  
Leopardus Pardalis ◽  
Assisted Reproduction Techniques ◽  
Vitro Fertilization

Objective: To describe the anatomy, morphology and physiology of the reproductive system of male jaguars, as well as assisted reproduction techniques. Methodology: A literature review on the anatomy and morphology of the jaguar´s reproductive system, its physiological characteristics and assisted reproduction techniques were carried out to document relevant information on the topic. Results: With this review, basic aspects of the morphology of the reproductive system of the jaguars are disclosed, although scarce knowledge is available on their reproduction. The advances in the collection, evaluation and cryopreservation of semen of this feline are shown, in addition to assisted reproduction techniques such as artificial insemination and in vitro fertilization, which have a great potential to safeguard the species. Study limitations: The jaguar, an emblematic species of Latinamerica, is an endangered species, like other wild felids species as ocelot (Leopardus pardalis) and margay (Leopardus wiedii), which makes it necessary to have a national assisted reproduction program. However, for this to be possible, information about their reproductive physiology is necessary, which is complicated in wild animals and even more so because the reproductive mechanisms greatly differ between felids species. There is scarce information in this regard from its free-living or Mexican zoos, it is for this reason necessary to generate such information. Conclusions: It is necessary to continue working on designing protocols for artificial insemination and other assisted reproduction techniques such as in-vitro fertilization specifically for male Panthera onca.

scholarly journals DNA recovery from archived RDTs for genetic characterization of Plasmodium falciparum in a routine setting in Lambaréné, Gabon

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
The Trong Nguyen ◽  
Brice Nzigou Mombo ◽  
Albert Lalremruata ◽  
Erik Koehne ◽  
Rella Zoleko Manego ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Real Time ◽  
Real Time Pcr ◽  
Rural Areas ◽  
Large Scale ◽  
Genetic Material ◽  
Chloroquine Resistance ◽  
Dna Recovery ◽  
Parasite Populations ◽  
Pcr Targeting

Abstract Background Rapid diagnostic tests (RDTs) have been described as a source of genetic material to analyse malaria parasites in proof-of-concept studies. The increasing use of RDTs (e.g., in focal or mass screening and treatment campaigns) makes this approach particularly attractive for large-scale investigations of parasite populations. In this study, the complexity of Plasmodium falciparum infections, parasite load and chloroquine resistance transporter gene mutations were investigated in DNA samples extracted from positive RDTs, obtained in a routine setting and archived at ambient temperature. Methods A total of 669 archived RDTs collected from malaria cases in urban, semi-urban and rural areas of central Gabon were used for P. falciparum DNA extraction. Performance of RDTs as a source of DNA for PCR was determined using: (i) amplification of a single copy merozoite surface protein 1 (msp1) gene followed by highly sensitive and automated capillary electrophoresis; (ii) genotyping of the pfcrt gene locus 72–76 using haplotype-specific-probe-based real-time PCR to characterize chloroquine resistance; and, (iii) real-time PCR targeting 18S genes to detect and quantify Plasmodium parasites. Results Out of the 669 archived RDTs, amplification of P. falciparum nucleic materials had a success rate of 97% for 18S real-time PCR, and 88% for the msp1 gene. The multiplicity of infections (MOI) of the whole population was 2.6 (95% CI 2.5–2.8). The highest number of alleles detected in one infection was 11. The MOI decreased with increasing age (β = − 0.0046, p = 0.02) and residence in Lambaréné was associated with smaller MOIs (p < 0.001). The overall prevalence of mutations associated with chloroquine resistance was 78.5% and was not associated with age. In Lambaréné, prevalence of chloroquine resistance was lower compared to rural Moyen-Ogooué (β = − 0.809, p-value = 0.011). Conclusion RDT is a reliable source of DNA for P. falciparum detection and genotyping assays. Furthermore, the increasing use of RDTs allows them to be an alternative source of DNA for large-scale genetic epidemiological studies. Parasite populations in the study area are highly diverse and prevalence of chloroquine-resistant P. falciparum remains high, especially in rural areas.

Unprotected freezing of human spermatozoa exerts a detrimental effect on their oocyte activating capacity and chromosome integrity

Zygote ◽  
1996 ◽  
Vol 4 (04) ◽  
pp. 263-268 ◽  
Author(s):  
Andrei V. Rybouchkin ◽  
Paul De Sutter ◽  
Marc Dhont
Keyword(s):  
Assisted Reproduction ◽  
Detrimental Effect ◽  
Chromosomal Abnormalities ◽  
Ethanol Solution ◽  
Human Spermatozoa ◽  
Physical Factors ◽  
Reproductive Characteristics ◽  
Chromosome Integrity ◽  
The Stability ◽  
Mammalian Spermatozoa

SummaryThe influence of unprotected freezing of mammalian spermatozoa on their oocyte activating capacity and chromosome integrity is unknown. However, this type of sperm treatment has been used in assisted reproduction by intracytoplasmic sperm injection in cattle and humans. The mouse oocyte injection test was used to analyse the influence of unprotected freezing of human spermatozoa on their reproductive characteristics. Mouse oocytes were microinjected with intact human spermatozoa or spermatozoa treated with two cycles of unprotected freeze-thawing. Oocytes surviving the injection were either cultured without further treatment or exposed to ethanol solution to induce parthenogenetic activation. Both injected and activated oocytes were used for sperm chromosome analysis. The results revealed a significant reduction in oocyte activating capacity and a tenfold increase in the incidence of structural chromosomal abnormalities in human spermatozoa treated by unprotected freezing. We conclude that unprotected freezing of human spermatozoa has a detrimental effect on their reproductive characteristics. Our data also provide a new perspective on the stability of mammalian spermatozoa to physical factors and demonstrate the importance of detailed analysis of the stability of sperm structures for successful development of new approaches in assisted reproduction.

scholarly journals Animal Health Services: Extent of Availability in Some Selected Rural Areas of Bangladesh

2014 ◽  
Vol 12 (2) ◽  
pp. 173-177
Author(s):  
MH Rahman ◽  
S Rana
Keyword(s):  
Health Services ◽  
Artificial Insemination ◽  
Rural Areas ◽  
Animal Health ◽  
Veterinary Services ◽  
Livestock Farmers ◽  
High Level ◽  
Almost All ◽  
Quantitative And Qualitative Methods ◽  
Animal Health Services

The main objective of the study was to assess the nature and extent of availability of animal health services received by the rural livestock farmers. The research was conducted in four upazilas namely Companyganj of Sylhet, Modhukhali of Faridpur, Mathbaria of Pirojpur and Mithamoin of Kishoreganj districts. Data were collected by using both quantitative and qualitative methods from a sample of 480 farmers during April to September 2010. Almost all of the farmers had high level of availability of veterinary services in relation to treatment of diseases and availability of medicine. Only few(6 percent) received services regarding artificial insemination. Percentage of respondents receiving services on vaccination (32 percent) and surgery and obstetrics (22 percent) were also moderate. The MFTS project beneficiaries received all services with higher frequencies than those of the non-beneficiaries except from the availability of medicine.The farmers living near upazila headquarter received better services than those living far from the upazila headquarters.DOI: http://dx.doi.org/10.3329/bjvm.v12i2.21281 Bangl. J. Vet. Med. (2014). 12 (2): 173-177 

scholarly journals ANXIETY & DEPRESSION;

2017 ◽  
Vol 24 (04) ◽  
pp. 589-595
Author(s):  
Muslim Ali Lakhiar ◽  
Shaheen Ahmed Mughal ◽  
Motti Ram Bhattia ◽  
Awais Bashir larik
Keyword(s):  
Rural Areas ◽  
Depression Scale ◽  
Female Gender ◽  
Health Sciences ◽  
Anxiety And Depression ◽  
Rural Environment ◽  
Psychiatric Evaluation ◽  
First Year ◽  
Cross Sectional ◽  
Environment Study

Objectives: To determine the prevalence of Anxiety & Depression among thestudents of Peoples University of Medical and Health Sciences for Women Nawabshah and toascertain the relative impact of rural environment. Study Design: Cross-sectional, questionnairebasedsurvey. Setting: Peoples University of Medical & Health Sciences for Women (PUMHSW),Nawabshah. Methods: This is the only medical university for female students located in theinterior of Sindh. The students belong to various rural districts of the Sindh Province. Aftertaking verbal consent the questionnaire of the Aga Khan University Anxiety & Depression Scale(AKUADS) Urdu version was administered to the students from first year to the final year. A scoreof ≥20 on AKUADS was used as a cut-off for the presence of anxiety and depressive symptoms.The data collected was tabulated and analyzed using SPSS 19. Results: Out of a total of 1035students in a five year MBBS program at PUMHSW, 851 students responded &agreed toparticipate. The response rate was 82.2%. According to the AKUADS 66.7% of female studentswere found to have anxiety and depression. Majority of the students were originally from variousrural districts of Sindh. The Prevalence of anxiety and depression among students of 1st year,2nd year, 3rd year, 4th year and 5th year was 71.1%, 76.9%, 53.7%, 70.7% and 63.6% respectively.It was significantly higher in 1st year and 2nd year as compared to 3rd, 4th and 5thyear (p<0. 05)students. Those aged <21 years were most vulnerable (p<0.025).Marital status and eitherliving at home or in hostel was statistically not significant (p<0.922 and p<0.596 respectively).None of the students were using antidepressants nor they ever visited a psychiatrist before.All students were attending lectures and hospital postings regularly. Conclusion: Our studyindicates that a high proportion of female medical students particularly those belonging tothe rural areas of Sindh suffer from anxiety and depression during the course of their medicaleducation. Female gender, younger age and the rural environment appears to have a profoundnegative impact. The overall prevailing situation warrants a proper psychiatric evaluation ofthese students so that remedial measures can be undertaken whenever necessary.

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