scholarly journals Evaluation of osteointegrative and osteoinductive properties of silicon doped ceramics in a model of rabbit’s femur fractures

2018 ◽  
pp. 44-53 ◽  
Author(s):  
M. Rublenko ◽  
V. Chemerovsky ◽  
V. Vlasenko ◽  
N. Ulyanchich
Keyword(s):  
Bone Tissue ◽  
Bone Defect ◽  
Inflammatory Reaction ◽  
Tricalcium Phosphate ◽  
Soft Tissues ◽  
Cartilage Tissue ◽  
Injured Limb ◽  
X Ray ◽  
Reparative Osteogenesis ◽  
Experimental Group

Bone regeneration is one of the most complex and unique types of tissue regeneration, although quite long in time, comparatively, for example, with soft tissues, but provides the complete identity of the damaged site with normal bone. The most complex fractures are fragmentation, which can be occurs within wide range - 25-60% of the total number of all fractures. In such cases, due to the loss of contact with soft tissues, the fragments lose blood supply and regeneration, which leads to different bone size defect. This condition cause limitation of the main mechanisms of bone consolidation – endoostal and intramembrane ossification. In this regard, a strategic medical treatment is the replacement of bone defect with biological or synthetic material, which creates a site for the processes of reparative osteogenesis. The most widespread combined biocompatible materials in the various combinations of β-tricalcium phosphate and hydroxyapatite ("Maxresorb®", "Perossal®", "calc-i-oss®CRYSTAL", "easy-graft®CRYSTAL"), or composite composites based on bioactive and biogenic materials: hydroxylapatite + collagen (Biostite, Collagraft, Avitene, Collola, Hapkol, Collapan, MP Composite); hydroxylapatite + tricalcium phosphate + collagen ("Hydroxyapol", "Collapolum"); hydroxylapatite + collagen + sulfated glycosaminoglycans ("Biomatrix", "Osteomatrix", "Bioimplant"). Unfortunately, in veterinary medicine osteotropic materials developed for humane medicine are used only. Recently, a separate group of biocompatible composites based on the combination of hydroxyapatite with β-tricalcium phosphate, doped with magnesium, sodium, potassium, zinc, copper, aluminum, strontium, silicon, germanium, in order to provide them with specific properties - antibacterial, osteoinductive, antitumor, immunomodulating, etc. However, the spectrum of biological effects of these ions on bone metabolism is extremely diverse, and therefore the use of composite ceramics doped with microelement ions requires a comprehensive clinical and experimental justification. The purpose of the study is to evaluate the osteointegration and osteoinductive properties of ceramics based on hydroxyapatite and β-tricalcium phosphate doped with silicon for model fractures of the femur in rabbits. The work is done on rabbits of Californian breed at the age of 3 months. and a weight of about 2.5 kg. To substantiate the ceramics GTlKg-2, 2 groups of 10 rabbits were formed in each, in which model bone defects were formed in the distal parts of the hip dysthymia. Animals of the experimental group defects filled with granules of ceramics. In the rabbits of the control group, the defect was left to heal under a blood clot. Animals were extracted from the experiment at the 21st and 42nd day. X-ray and histomorphological studies were performed. On the 21st day of reparative osteogenesis, rabbits of all groups fully rested on the injured limb, signs of inflammatory reaction were absent in the experimental group, and the control marked the pronounced seal of the periosteum across the entire surface of the femur. It should be noted that hydroxyapatite ceramics does not possess x-ray contrast properties. On the 42nd day of regeneration of rabbits both groups fully rested on injured limb, signs of inflammatory reaction of soft tissues in the area of injury were absent. Radiologically, in animals of the experimental group in the place of bone defect, spot osteosclerosis was detected in the form of a clearly defined white heel, opposite to which the contour of the periosteum was sealed. At the same time, on the control X-rays, along with a well-defined, but more elongated septum of the periodontal, revealed a bone marrow panossus at the site of the injury, with a clearly defined extension of the eclipse. Substantially complemented macromorphological picture of bone biopsy. In particular, in the case of replacement of bone defect GTlKg-2, at the 21st day in the traumatic areas a limited and moderate periosteal reaction was noted. Along with this, in control animals, in this period, it was not completely replaced by fibrous cartilaginous tissue, as evidenced by its craterial appearance. Histologically, in the control animals, the bone defect formed a cartilage tissue along the periphery, and the bone beams, which were at a certain distance from the place of the defect, were at the stage of resorption. In the case of its replacement granules GTlKg-2 formed bone-ceramic regenerate, that is, the intervals between the granules are filled with bone tissue. The obtained results give grounds to consider that GTlKg-2 contributes to the formation of bone tissue due to its osteointegration and osteoinductive properties. Key words: reparative osteogenesis, osteointegration, osteocytes, osteoblasts, hydroxyapatite composite with β-tricalcium phosphate, doped with silicon.

scholarly journals Histomorphological characteristics of bone replacement in rabbits with hydroxyapatite ceramics and Platelet-Rich Fibrin

2021 ◽  
Vol 23 (102) ◽  
pp. 43-52
Author(s):  
S. М. Shevchenko ◽  
M. V. Rublenko ◽  
N. V. Ulyanchich ◽  
P. P. Klymenko
Keyword(s):  
Bone Tissue ◽  
Tricalcium Phosphate ◽  
Blood Clot ◽  
Compact Bone ◽  
Control Group ◽  
Bone Lesions ◽  
Platelet Rich Fibrin ◽  
Reparative Osteogenesis ◽  
Hydroxyapatite Ceramics ◽  
Experimental Group

The results of a histomorphological study of bone regenerates in rabbits after osteosubstitution with platelet-rich fibrin and its combination with hydroxyapatite granules with β-tricalcium phosphate in spongy and compact bone tissue at different periods of reparative osteogenesis are presented. Three groups of rabbits were formed. In the control group, the defects were left to heal under the blood clot. Bone lesions in the first experimental group were filled with platelet-rich fibrin, in the second – with a combination of platelet-rich fibrin and hydroxyapatite with β-tricalcium phosphate. All animals were kept in the same conditions of feeding and housing, had unlimited access to water. During the study, the rabbits were monitored clinically. The animals were taken out of the experiment on the 14th, 21st and 42nd days, samples of bone tissue were taken, they were fixed, decalcified, dehydrated in alcohols of increasing concentration and embedded in paraffin. In the presented study, it is most likely that the newly formed bone tissue is formed precisely due to osteoinduction in the experimental groups. When using granules of hydroxyapatite and β-tricalcium phosphate with platelet-rich fibrin, bone regenerate is between the composite granules and is not associated with contact with the maternal bone. Its cells appear in different places of the defect. In the spongy bone tissue on the 21st day, regeneration proceeded more fully and faster in the second experimental group, as evidenced by the significant density of cells of the osteoblastic row, the thickness of the bone trabeculae and their volume, filling the site of the defect. On the 42nd day, in the second experimental group, when using a combination of autobiomaterial and hydroxyapatite granules with β-tricalcium phosphate, the regenerate contained a significantly larger number of osteogenic cells in the thickness of the trabeculae, which indicated a more intensive course of reparative osteogenesis in comparison with the first experimental and control groups. In the compact bone on day 21, regeneration was more complete and faster in the second experimental group. On the 42nd day, according to the degree of maturity of the bone regenerate, calcium-phosphate ceramics in combination with platelet-rich fibrin optimizes reparative osteogenesis most clearly. According to the degree of intensity of the osteoregeneration process, the groups can be placed in the following sequence: control ˂PRF˂PRF+HA/β-TCP–700. According to histomorphological characteristics, the combination of hydroxyapatite with β-tricalcium phosphate and platelet-rich fibrin gives greater osteoinduction to the composite material, which is confirmed by the high cell density, namely of osteoblasts and osteocytes. The use of PRF in combination with other materials may become promising for the correction of reparative osteogenesis in conditions of limited or reduced regenerative potential of bone tissue.

Biological Response to Wollastonite Doped α-Tricalcium Phosphate Implants in Hard and Soft Tissues in Rats

2008 ◽  
Vol 396-398 ◽  
pp. 7-10 ◽  
Author(s):  
Ana Maria Minarelli Gaspar ◽  
Sybele Saska ◽  
R. García Carrodeguas ◽  
A.H. De Aza ◽  
P. Pena ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Bone Formation ◽  
Bone Tissue ◽  
Tricalcium Phosphate ◽  
Rattus Norvegicus ◽  
Soft Tissues ◽  
Subcutaneous Tissue ◽  
Biological Response ◽  
New Bone Formation ◽  
Rat Bone

The biological response following subcutaneous and bone implantation of β-wollastonite(β-W)-doped α-tricalcium phosphate bioceramics in rats was evaluated. Tested materials were: tricalcium phosphate (TCP), consisting of a mixture of α- and β-polymorphs; TCP doped with 5 wt. % of β-W (TCP5W), composed of α-TCP as only crystalline phase; and TCP doped with 15 wt. % of β-W (TCP15), containing crystalline α-TCP and β-W. Cylinders of 2x1 mm were implanted in tibiae and backs of adult male Rattus norvegicus, Holtzman rats. After 7, 30 and 120 days, animals were sacrificed and the tissue blocks containing the implants were excised, fixed and processed for histological examination. TCP, TCP5W and TCP15W implants were biocompatible but neither bioactive nor biodegradable in rat subcutaneous tissue. They were not osteoinductive in connective tissue either. However, in rat bone tissue β-W-doped α-TCP implants (TCP5W and TCP15W) were bioactive, biodegradable and osteoconductive. The rates of biodegradation and new bone formation observed for TCP5W and TCP15W implants in rat bone tissue were greater than for non-doped TCP.

scholarly journals Chondroplasty Efficacy of Bone Matrix

2019 ◽  
Vol 2 (1) ◽  
pp. 8-13
Author(s):  
Iryanov Yu.M ◽  
Kiryanov N.A
Keyword(s):  
Articular Cartilage ◽  
Bone Matrix ◽  
Cartilage Lesion ◽  
Cartilage Tissue ◽  
Rejection Reaction ◽  
X Ray ◽  
Osteoclastic Resorption ◽  
Male Wistar Rats ◽  
Experimental Group ◽  
Joint Material

Aim To study the chondroplasty efficacy of the bone matrix obtained using an original technology in restoring cartilage defect of the knee joint. Material and Methods Marginal defects were modeled on the surface of the distal end of the femur in 40 adult male Wistar rats. The bone matrix obtained using an original technology was implanted in the damaged area in animals of the experimental group. Material was investigated by means of light microscopy, transmission and scanning electron microscopy, and electron probe X-ray microanalysis. Results It was found that the bone matrix implanted did not cause an immune rejection reaction, activated reparative chondrogenesis for a prolonged period. In the area of articular cartilage lesion, the regenerate acquiring cellular and histochemical characteristics of the hyaline cartilage tissue was formed. The chondroinductive properties for the bone matrix were ensured by localized growth factors and morphogenetic proteins released during osteoclastic resorption. Conclusion The application of the bone matrix as a stimulator of chondrogenesis is theoretically reasonable and has a good perspective in treatment of damages and diseases of the articular cartilage.

scholarly journals Radiographic, macromorphological and hematological evaluation of hydroxyapatite ceramics with diff erent physicochemical properties

2020 ◽  
pp. 140-152
Author(s):  
V. Chemerovsky
Keyword(s):  
Composite Materials ◽  
Bone Tissue ◽  
Blood Clot ◽  
Hematological Parameters ◽  
Indirect Evidence ◽  
External Jugular Vein ◽  
Control Group ◽  
Reparative Osteogenesis ◽  
Hydroxyapatite Ceramics ◽  
Experimental Group

Treatments of fragmentary fractures in case of loss regenerative potential of bone tissue require usingdiff erent composite materials. Among all of them, ceramics based on synthetic hydroxyapatite and tricalcium phosphate are considered promising. The reparative osteogenesis in spongy and compact rabbit’s bone tissueusing 3 composite materials with diff erent physicochemical propertieswas studied. A reparative osteogenesiscontrolled by monitoring clinical, radiologic and macromorphologic parameters. Composite materials infl uence on the organism was studied by determining the dynamics of hematological parameters throughout the study period. To realize this scheme was formed a 3 experimental groups and one control group of rabbits. The created defects of animals of fi rst experimental group were fi lled out with GT + α-TKF-500, thedefects of second oneanimals group fi lled out with GTlKl-2, and defects ofthird oneanimals group fi lled out with GTlKg-700. The GTlKl-700materials were doped with silicon. In control animals, bone defects were allowed to heal under a blood clot. During the studies, all animals were located in the same environment conditions. A blood samples for hematological investigation was taken from the external jugular vein before anesthesia and at the 3rd, 7th, 14th, 21st and 42nd day after surgery. X-ray examination was performed on 14th, 21st, and 42nddays.The animals were removed from the experiment on 21stand 42nd days. The samples were examined by macromorphologic method. By radiologic examination was found that composite materials had osteoconductive properties except the silicondoped example which hadosteoinductive properties. The samples of compact bone tissue of 1st and 3rd experimental groups characterized by formation of punctate osteosclerosis with a compacted contour of the periosteumon the 42nd day. But the bone samples of 2nd experimental group had compacted contours of the periosteum only. However, in the cancellous bone tissue on the 42nd day in the animals of the 1st experimental group developed point osteosclerosis, which visualized as individual granules of the composite.But in the animals of 2nd group thewhite spot which was outlined shape and homogeneous structure at the defect was found. The bone samples of 3rd animals group shoved a clearly limited point osteosclerosisforming were was found ceramic granules. In case using ofGT + α-TKF-500, a strong connection of granules from the formed bone tissue and without any periosteal growthswas noted. Using GTlKl-2 places of defects were identical to not injured sites of radial bones. Using GTlKg-700 shows that bone defect is fi lled to the level of the plane of the bone surface and covered with a periosteum without visible growths. The granules of the composite are evenly distributed in the regenerate and associated with bone tissue. The analysis of hematological parameters did not reveal any fundamental diff erences, but the use of hydroxyapatite implants is not accompanied, in contrast to spontaneous reparative osteogenesis, the development of leukocytosis, which indicates a moderate course of its infl ammatory reaction. But the latter is accompanied by a platelet reaction, the most pronounced when using implants, which is probably due to the infl uence of platelet factors and is indirect evidence of early osteoblastic reaction. Key words: fractures, rabbits, erythrocytes, leukocytes, platelets, hemoglobin.

scholarly journals Lead – factors affecting its content in bone tissue

2020 ◽  
Vol 66 (4) ◽  
pp. 23-29
Author(s):  
Barbara Brodziak-Dopierała
Keyword(s):  
Nervous System ◽  
Mental Retardation ◽  
Bone Tissue ◽  
Human Body ◽  
Soft Tissues ◽  
Factors Affecting ◽  
X Ray ◽  
Human Bones ◽  
Wide Range ◽  
Living Organisms

AbstractLead is an element whose presence in the environment results mainly from man’s activities. It infiltrates the bodies of living organisms in many ways; in the human body, it has a multidirectional activity, causing anemia, kidney damage, mental retardation in children, genotoxicity, impaired immune function, damage to the nervous system and an inhibition of heme synthesis. Lead also affects bones; its concentrations there, in contrast to soft tissues, are dependent on exposure and increase throughout ones lifespan. This element is the most often studied and described metal in the bone tissue. The aim of this study was to determine the factors that influence the content of lead in the bone tissue and trace its content in this tissue based on literature data. The analysis of the collected literature showed an incredibly wide range of lead content in human bones was observed. This could be due to the different methods of determination (AAS, ICP-AES, K x-ray fluorescence) and the weight of the sample (dry or wet).

scholarly journals Results of the Study of Bone Tissue Density in Patients of the Study Groups at the Restoration of Bone Defects by Various Osteoplastic Materials

2021 ◽  
Vol 6 (2) ◽  
pp. 158-162
Author(s):  
A. V. Bambuliak ◽  
◽  
N. B. Kuzniak ◽  
R. R. Dmitrenko ◽  
S. V. Tkachik ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Bone Density ◽  
Bone Tissue ◽  
Bone Defect ◽  
Maxillofacial Surgery ◽  
Bone Defects ◽  
X Ray ◽  
Group A ◽  
X Ray Computed ◽  
Group B

The restoration processes of damaged or lost bone tissue are an important and topical issue of surgical dentistry and maxillofacial surgery today. According to statistics, diseases that are accompaning by destructive changes in bone tissue occupy one of the dominant places among the nosologies of MFA. Along with the use of updated methods of surgical technique, both before and now the problem of choice of bone-plastic material is relevant, because it is known that osteoplasty opens up great opportunities for complete rehabilitation of patients. The purpose of the study was to determine the effectiveness of our developed osteoplastic composition for the restoration of bone defects in dentistry. Materials and methods. X-ray computed tomography to determine bone density was performed in 26 patients with bone defect replacement "Kolapan-L" (group A), 28 people during augmentation of our proposed osteoplastic composition "Kolapan-L" + multipotent mesenchymal stromal cells + platelet-rich plasma) (group B), and 25 patients where the healing of the bone defect occurred spontaneously (group B). X-ray computed tomography was performed on a 16-slice spiral computed tomography Siemens Somatom Emotionc. Image processing was performed using the program "Dicom". Statistical processing of research results was carried out using conventional methods of variation statistics. Results and discussion. After 1 year of research, the average value of the bone density in patients of group B was probably higher: 1.5 times and 1.8 times relative to the corresponding data in groups A and B. At the same time, the minimum value of the bone density in patients of subgroups A and B was 1.5 times and 1.8 times lower than in persons of group B (p <0.01, p1 <0.01). At the same time, the maximum values of the bone density in patients of group B, where the augmentation of bone defects was performed using our proposed composition, were 1.4 times (p <0.01) and 1.8 times (p <0.05, p1 <0.01) higher than in group A, in the replacement of bone defects "Kolapan-L" and in group B, where the healing of the bone defect was spontaneous, respectively. It was found that in patients of group B after 12 months of studies, the average density of osteoregeneration was 1036.69±55.53 (HU), which was 1.5 times and 1.8 times more than in group A (p <0.01) and in group B patients (p <0.05, p1 <0.01) respectively. Conclusion. The use of tissue equivalent of bone tissue, proposed by us to replace a bone defect based on multipotent mesenchymal adipose tissue cells, contributed to the maximum increase in bone density, with a slightly lower effect of increasing bone density in the augmentation of bone defects

scholarly journals Bone Tissue Response to Endomethasone Implanted into the Rat Mandible / Reakcija koštanog tkiva na endometazon implantiran u mandibulu pacova

2015 ◽  
Vol 32 (2) ◽  
pp. 155-164 ◽  
Author(s):  
Marija Nikolić ◽  
Jovanka Gašić ◽  
Aleksandar Mitić ◽  
Jelena Popović ◽  
Aleksandra Radovanović ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Bone Healing ◽  
Inflammatory Reaction ◽  
Microscopic Analysis ◽  
Mental Foramen ◽  
Tissue Response ◽  
Male Rats ◽  
Control Group ◽  
Rat Mandible ◽  
Experimental Group

SUMMARY The aim of the paper was to investigate the tissue response to the implantation of "Endomethasone" (Septodont) in artificially prepared defect in the rat mandible. Sixteen Wistar male rats were used for the experiment. After anesthesia, a defect between the midline and the mental foramen of the left mandible was made (diameter 1.4 mm, 1.6 mm depth) using sterile steel burs. In the defect of the experimental group (12 animals) “Endomethasone“ was implanted while the defect in the control group (4 animals) was left to heal spontaneously. One half of animals of both groups was sacrificed after fifteen days, and the second half after sixty days. The samples consisted of the defect and the surrounding bone. After routine decalcination and processing, the samples were embedded in paraffin, and microscopic preparations were made, on the basis of which a microscopic analysis was performed. Fifteen-day-old specimens showed the early signs of bone resorption, as well as granulated connective tissue, hyperemic blood vessels, fibrin exudate to a lesser extent, with the difference that inflammatory infiltrates were less present in the experimental group. Sixty-day-old specimens demonstrated the degree of bone healing; tissue filling with newly formed bone was significantly more advanced in the control group. Experimental group showed a sign of delay with significant presence of callus tissue that more in the form of focus showed a deposition of the young bone tissue. Endomethasone masks the inflammatory reaction which follows operational procedures. In the long run, according to the results of the present research, Endomethasone can interfere with bone healing by causing delayed inflammatory reaction

scholarly journals Morphological Characteristics and Correction of Long Tubular Bone Regeneration under Chronic Hyperglycemia Influence

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Yevhenii S. Dudchenko ◽  
Olena S. Maksymova ◽  
Vasyl S. Pikaliuk ◽  
Dmytro V. Muravskyi ◽  
Ludmila I. Kyptenko ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Tissue ◽  
Bone Defect ◽  
Morphological Characteristics ◽  
Control Group ◽  
Tubular Bone ◽  
Reparative Osteogenesis ◽  
Defect Site ◽  
Woven Bone ◽  
Chronic Hyperglycemia

Introduction. Unsatisfactory consequences of bone regeneration disorders in diabetes mellitus (DM) patients, their high prevalence, complication number, and difficulties in treatment require further study and deeper understanding of reparative osteogenesis mechanisms under chronic hyperglycemia and finding new effective and affordable approaches to their treatment. Therefore, the aim of our work was to study the histological, ultramicroscopic, and histomorphometric features of reparative osteogenesis in rats with chronic hyperglycemia (CH), as well as to investigate the possibility of platelet-rich plasma (PRP) use in a fracture area in order to correct the negative effects of CH on reparative osteogenesis processes. Study Object and Methods. The studies were performed on 70 white laboratory rats, mature males, which were divided into the following groups: control group, animals with posttraumatic tibial defect under conditions of CH exposure, rats with experimental CH that were administered with PRP into the bone defect, and animals for the assessment of glucose homeostasis and confirmation of simulated CH. Light microscopy was performed using an Olympus BH-2 microscope (Japan). Ultramicroscopic examination was performed using REM-102 scanning electron microscope. The statistical analysis was performed using SPSS-17 software package. Results. The formation of new bone tissue in animals with CH did not occur after two weeks. Only on the 30th day of reparative osteogenesis the newly formed woven bone tissue was 61.54% of the total regenerated area. It was less than the reference value by 22.89% (P<0.001). On the 14th day of reparative osteogenesis, the regenerated area in a group of animals with CH and PRP injection consisted of connective tissue by 68.94% (4.94% less than in animals with CH (P<0.001)) and woven bone tissue by 31.06%, (13.51% less than in the control group (P<0.001)). On the 30th day, the area of woven bone tissue in a regenerate of this group was less than that of the control group by 12.41% (P<0.001). Conclusion. Thus, chronic hyperglycemia contributes to inflammation delay within the bone defect site, which makes the process of reparative osteogenesis more prolonged. The results of chronic hyperglycemia effect on bone regeneration are also impairment of osteogenic cell proliferation and shift of their differentiation towards the fibrocartilage regenerate formation. The PRP corrects the negative impact of chronic hyperglycemia on reparative osteogenesis, promoting more rapid inflammatory infiltrate removal from the bone defect site and osteogenic beam formation and remodeling of woven bone into lamellar membranous bone tissue.

scholarly journals X-ray and macromorphological evaluation of reparative osteogenesis by implantation of hydroxyapatite composite doped with germanium

2020 ◽  
pp. 183-194
Author(s):  
T. Todosyuk
Keyword(s):  
Bone Fractures ◽  
Compact Bone ◽  
Control Group ◽  
X Ray ◽  
Reparative Osteogenesis ◽  
Defect Site ◽  
Hydroxyapatite Ceramics ◽  
Hydroxyapatite Composite ◽  
Ray Density ◽  
Experimental Group

Fragmentary fractures require not only the use of complex methods of osteosynthesis, but also the replacement of the defect site with implants to optimize reparative osteogenesis. A large number of osteosubstitute materials are used, including hydroxyapatite ceramics. To enhance its osteointegration properties, microelements with osteoinductive properties are added. The aim of the work is X-ray and macromorphological evaluation of the influence of hydroxyapatite ceramics doped with germanium on reparative osteogenesis in model fractures of the femur and radius in rabbits. Model defects were formed in the radial diaphysis and femur metaphysis in rabbits with a drill with a diameter of 3 mm and 4.2 mm, respectively. Anesthesia included acepromazine, thiopenate, and lidocaine infiltration anesthesia. In the experimental group (n=12) defects were replaced by granules of hydroxyapatite ceramics doped with germanium, and in the control group (n=12) – undoped. The use of hydroxyapatite ceramics doped with germanium is accompanied by a moderate course of the inflammatory-resorptive phase of reparative osteogenesis. At the same time on the radiographs of animals of the experimental group on the 14th day the periosteal reaction is moderate, and on the 30th day it is barely noticeable with increased radiological density. In control animals after trauma to the compact bone proximal and distal to its location showed a thickened and compacted periosteum with a contrasting composite material. On the 60th day in the cancellous bone of experimental animals, the area of injury acquired an X-ray density close to normal, in the control – it remained elevated. Hydroxyapatin composite doped with germanium acquires osteoinductive properties and may be promising for the replacement of bone defects and correction of reparative osteogenesis in animals. Key words: bioceramics, germanium, bone fractures, compact and spongy bone tissue, rabbits.

scholarly journals EFFECTS OF DIABETES MELLITUS ON REPARATIVE OSTEOGENESIS

2019 ◽  
Vol 72 (9) ◽  
pp. 1723-1726
Author(s):  
Oksana K. Melekhovets ◽  
Vira D. Tovazhnyanska ◽  
Iryna I. Yakovtsova
Keyword(s):  
Soft Tissues ◽  
Callus Formation ◽  
Cartilage Tissue ◽  
Adult Rats ◽  
Reparative Osteogenesis ◽  
Morphological Studies ◽  
Morphological Evaluation ◽  
Chronic Hyperglycemia ◽  
Tibia Diaphysis ◽  
Group 2

Introduction: Chronic hyperglycemia as the main link in DM pathogenesis leads to systemic vessels and nerves lesion with chronic bone complications development consequently. The aim: To evaluate influence of hyperglycemia on reparative osteogenesis after perforated tibial fracture in rats. Materials and methods: A total of 30 white adult rats were subdivided into two groups: 15 healthy rats in Group 1 (control) and 15 rats with alloxan induced hyperglycemia in Group 2 (investigated) and were carried out of experiment on the 10th, 20th and 30th day after the fracture. Hyperglycemia in rats was verificated as the postprandial glycemic rate ≥ 8,0 mmol/l. Tibia diaphysis fracture was modeled by a cylindrical defect with a diameter of 2 mm with portable frezer. Morphological evaluation. A complex morphological studies included histological, morphometric and immunohistochemical examination. Results: This is confirmed by an increase in MMP-9 expression in connective tissue, a decrease in TGF-β expression in all phases, an increase in the expression of CD3 and CD20 and a marked decrease in the expression of all vascular markers. During hyperglycemia, incomplete blood supply to the tissues occurs, necrosis of bone and soft tissues develop in the area of the fracture, the reparative reaction slows down considerably and manifests itself in the development of fibrous and, less commonly, cartilage tissue. Conclusions: In hyperglycemia rats, there was a delay in the callus formation, a decrease in proliferation and ossification, and a slowdown in the processes of angiogenesis.

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