scholarly journals Efficacy of Different C18 HPLC Analytical Columns in the Analysis of Fumonisins B1 and B2 in Different Matrices

2021 ◽  
Vol 12 (2) ◽  
pp. 1721-1734
Keyword(s):  
Fusarium Verticillioides ◽  
Fungal Culture ◽  
Particle Sizes ◽  
Limit Of Quantification ◽  
Porous Particles ◽  
Hplc Column ◽  
Products Analysis ◽  
Core Column ◽  
Different Dimensions ◽  
Fumonisins B1 And B2

Fumonisins B1 and B2 are carcinogenic and commonly contaminate corn and corn-based products. Analysis of such toxins using C18 HPLC column is officially accredited but still unknown if all column types can effectively separate FB1 and FB2 or not. The present study evaluated the efficiency of 5 analytical columns with different dimensions, particle sizes, and porosities to determine these toxins in both agar cultures of Fusarium verticillioides and cornflakes. Interestingly, the traditional column 150mm of length with 5µm porous particles had close retention times to those of the short-fused core column 75mm of length with 2.7 µm reflecting in time and solvents saving. Using Sep-Pack C18 for clean-up played an important role in enhancement the limit of quantification (LOQ) for cornflake samples (5-13.7 and 16.1-39 µg kg-1 for FB1 and FB2, respectively). However, it was relatively higher for fungal culture samples that were not passed through the cleaning-up step (11.5-16 and 28.1-46.3 µg kg-1 for FB1 and FB2, respectively). Overall, the lowest LOQ was obtained using the shorter fused core column. Finally, using such clean-up in the extraction of FB1 and FB2 from spiked cornflakes samples gave good recoveries (>80%) using all tested columns.

Structure-, dimension-, and particle size-engineering toward highly efficient supported nanoparticulate metal catalysts

2018 ◽  
Vol 6 (38) ◽  
pp. 18561-18570 ◽  
Author(s):  
Yangke Long ◽  
Yixuan Huang ◽  
Xiaowen Shi ◽  
Ling Xiao
Keyword(s):  
Particle Size ◽  
Metal Catalysts ◽  
Cobalt Catalysts ◽  
Particle Sizes ◽  
Efficient Catalyst ◽  
Cobalt Particle ◽  
Nitrogen Doped ◽  
Different Dimensions ◽  
Structure Dimension ◽  
Nitrogen Doped Carbon

Among the nitrogen doped carbon-supported nanoparticulate cobalt catalysts of different dimensions and cobalt particle sizes screened, 2D Co–N–C-0.5 is identified as the most efficient catalyst.

Activity behavior of a HPLC column including α-chymotrypsin immobilized monosized-porous particles

2004 ◽  
Vol 516 (1-2) ◽  
pp. 125-133 ◽  
Author(s):  
Z Bilici ◽  
S.T Camli ◽  
E Unsal ◽  
A Tuncel
Keyword(s):  
Porous Particles ◽  
Hplc Column ◽  
Activity Behavior

Molecularly imprinted polydopamine nano-layer on the pore surface of porous particles for protein capture in HPLC column

2013 ◽  
Vol 404 ◽  
pp. 117-126 ◽  
Author(s):  
Ali Nematollahzadeh ◽  
Akbar Shojaei ◽  
Mohammad J. Abdekhodaie ◽  
Börje Sellergren
Keyword(s):  
Pore Surface ◽  
Molecularly Imprinted ◽  
Porous Particles ◽  
Protein Capture ◽  
Hplc Column

scholarly journals Slow viscous flow of two porous spherical particles translating along the axis of a cylinder

2018 ◽  
Vol 861 ◽  
pp. 643-678 ◽  
Author(s):  
Xin Yao ◽  
Chyi Huey Ng ◽  
Jia Rui Amanda Teo ◽  
Marcos ◽  
Teck Neng Wong
Keyword(s):  
Flow Field ◽  
Particle Interaction ◽  
Cylindrical Tube ◽  
Separation Distance ◽  
Spherical Particles ◽  
Particle Sizes ◽  
Slow Viscous Flow ◽  
Different Dimensions ◽  
Freely Moving ◽  
Cylindrical Harmonics

We describe the motion of two freely moving porous spherical particles located along the axis of a cylindrical tube with background Poiseuille flow at low Reynolds number. The stream function and a framework based on cylindrical harmonics are adopted to solve the flow field around the particles and the flow within the tube, respectively. The two solutions are employed in an iterated framework using the method of reflections. We first consider the case of two identical particles, followed by two particles with different dimensions. In both cases, the drag force coefficients of the particles are solved as functions of the separation distance between the particles and the permeability of the particles. The detailed flow field in the vicinity of the two particles is investigated by plotting the streamlines and velocity contours. We find that the particle–particle interaction is dependent on the separation distance, particle sizes and permeability of the particles. Our analysis reveals that when the permeability of the particles is large, the streamlines are more parallel and the particle–particle interaction has less effect on the particle motion. We further show that a smaller permeability and bigger particle size generally tend to squeeze the streamlines and velocity contour towards the wall.

Natural Occurrence of Fusarium Species, Fumonisin Production by Toxigenic Strains, and Concentrations of Fumonisins B1 and B2 in Conventional and Organic Maize Grown in Spain

2007 ◽  
Vol 70 (1) ◽  
pp. 151-156 ◽  
Author(s):  
AGUSTÍN ARIÑO ◽  
TERESA JUAN ◽  
GLORIA ESTOPAÑAN ◽  
JOSÉ F. GONZÁLEZ-CABO
Keyword(s):  
High Performance ◽  
Fusarium Species ◽  
Fusarium Verticillioides ◽  
Fumonisin B1 ◽  
Farming System ◽  
Natural Occurrence ◽  
Significant Difference ◽  
The Difference ◽  
Organic Farming System ◽  
Fumonisins B1 And B2

Sixty samples of corn from both conventional and organic farms were tested for internal fungal contamination. Molds were identified to genus, and those belonging to the genus Fusarium were identified to species. Twenty isolates of Fusarium verticillioides were tested with a high-performance liquid chromatography–naphthalene dicarboxaldehyde–fluorescence method for their ability to produce fumonisins B1 and B2. The internal fungal infection in organic maize (63.20%) was significantly higher than that in conventional maize (40.27%) (P < 0.05). However, the distribution of fungal genera indicated a significantly higher prevalence of Fusarium in conventional (34.93%) than in organic (18.15%) maize, making Fusarium the predominant fungus in conventional maize. This difference in mold distribution between organic and conventional maize was attributed to the difference in cultivation system. The dominant Fusarium species in both conventional and organic samples was F. verticillioides. There were no significant differences in the ability of 20 selected isolates of F. verticillioides to produce fumonisins on conventional or organic corn. Up to 13.3% of the conventional corn samples contained fumonisins B1 and B2 at mean concentrations of 43 and 22 ng/g, respectively. Organic corn samples had somewhat lower levels of contamination: 35 ng/g fumonisin B1 and 19 ng/g fumonisin B2 (P > 0.05). The organic farming system, with well-balanced crop rotation, tillage, and compost fertilization, produced corn that was less likely to be contaminated with Fusarium species, although no significant difference in fumonisin concentrations was found between the two types of contaminated corn.

The impact of silica gel pore and particle sizes on HPLC column efficiency and resolution for an immobilized, cyclodextrin-based, chiral stationary phase

2010 ◽  
Vol 33 (17-18) ◽  
pp. 2582-2589 ◽  
Author(s):  
Qing Qin ◽  
Sheng Zhang ◽  
Wei-Guang Zhang ◽  
Zhen-Bin Zhang ◽  
Ya-Jin Xiong ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Silica Gel ◽  
Chiral Stationary Phase ◽  
Particle Sizes ◽  
Column Efficiency ◽  
Hplc Column ◽  
The Impact

scholarly journals INHIBITORY CONCENTRATION DETERMINATION OF FUNGI CAUSING POSTHARVEST DISEASES ON RAMBUTAN BY SOME CHEMICAL COMPOUNDS AND TWO STRAINS OF LACTIC BACTERIA

2020 ◽  
Vol 1 (40) ◽  
pp. 121-130
Author(s):  
Yen Thi Ngoc Thach ◽  
Thanh Van Nguyen ◽  
Phong Van Nguyen
Keyword(s):  
Room Temperature ◽  
Fusarium Verticillioides ◽  
Chemical Compounds ◽  
Calcium Lactate ◽  
Fruit Rot ◽  
Potassium Sorbate ◽  
Fungal Culture ◽  
Postharvest Diseases ◽  
Fungal Strains ◽  
Lactic Bacteria

The study surveyed on the inhibition of post-harvest fungi on rambutan by Presim, calcium lactate, Citribio,potassium sorbate and two strains of Lactbacillus plantarum, Lactobaillus fermentum at 13oC and room temperature (28oC ± 2 ). The experiment was carried out by fungal culture method in the inhibitoryenvironment at the concentration range of 0.05% – 0.45% (for chemical compounds), and by co-cultivation method in a double medium between colony and spores fungal and lactic acid bacteria. The resultsshowed that 7 strains caused rambutan fruit rot to be harvested after harvesting include Lasiodiplodia psedotheobromae, Fusarium verticillioides, Phomopsis mali, Lasmenia sp., Gliocephalotrichum cylindrosporum, Pestalotiopsis virgatula voucher, Pestalotiopsis clavispora are inhibited by presim and triobio concentrations are from 0.05% to 0.15%. The two compounds are calcium lactate (concentrations of 0.15% – 0.45% and potassium sorbate (0.02% – 0.05%) did not show inhibition for these seven strains. L. plantarum and L. fermentum) are highly inhibitory to colony and spores of 7 fungal strains, of which L. planterum has astronger inhibition capacity than L. fermentum in diameter inhibition is 30 – 75 mm at 13oC At room temperature (28oC ± 2) L. Fermentum doesn’t inhibit G. cylindrosporum. Of the seven fungal strains,there are five strains include F. verticillioides, P. mali, Lasmenia sp., G. cylindrosporum and P. virgatula voucher were inhibited with 2 strains of lactic bacteria higher than those of L. pseudotheobromae and P. clavispora.

Biogenesis of Catalase-Positive Rods in Excretory Duct Cells of Salivary Glands

1976 ◽  
Vol 34 ◽  
pp. 62-63
Author(s):  
Dwight K. Romanovicz ◽  
Jacob S. Hanker
Keyword(s):  
Light Microscopy ◽  
Salivary Glands ◽  
Submandibular Gland ◽  
Excretory Duct ◽  
Exocrine Glands ◽  
Duct Cells ◽  
Peroxidatic Activity ◽  
Microscopic Studies ◽  
Different Dimensions

The presence of catalase-positive rods (Fig. 1) of different dimensions, which frequently have a crystalline appearance by light microscopy, has been reported. They seem to be related to peroxisomes which were characterized morphologically and cytochemically in parotid and other exocrine glands of the rat by Hand in 1973. Our light microscopic studies of these spherical microbodies and rods of different sizes, stained by virtue of the peroxidatic activity of their catalase, indicate that they are almost entirely confined to the cells of the striated and execretory ducts of the submandibular gland in the mouse. The rods were usually noted only in the proximity of the ductal microbodies. The latter frequently showed a tendency to appear in linear close array, or even to be contiguous (Fig. 2). This suggested that the rods could be formed by the fusion of microbodies.

Characterization of carbides in M50NiL

1991 ◽  
Vol 49 ◽  
pp. 606-607
Author(s):  
L. S. Lin ◽  
K. P. Gumz ◽  
A. V. Karg ◽  
C. C. Law
Keyword(s):  
Temperature Effects ◽  
Base Composition ◽  
Lattice Parameter ◽  
Control Surface ◽  
Carbide Formation ◽  
Particle Sizes ◽  
Low Carbon ◽  
Fee Structure ◽  
Heat Treated

Carbon and temperature effects on carbide formation in the carburized zone of M50NiL are of great importance because they can be used to control surface properties of bearings. A series of homogeneous alloys (with M50NiL as base composition) containing various levels of carbon in the range of 0.15% to 1.5% (in wt.%) and heat treated at temperatures between 650°C to 1100°C were selected for characterizations. Eleven samples were chosen for carbide characterization and chemical analysis and their identifications are listed in Table 1.Five different carbides consisting of M6C, M2C, M7C3 and M23C6 were found in all eleven samples examined as shown in Table 1. M6C carbides (with least carbon) were found to be the major carbide in low carbon alloys (<0.3% C) and their amounts decreased as the carbon content increased. In sample C (0.3% C), most particles (95%) encountered were M6C carbide with a particle sizes range between 0.05 to 0.25 um. The M6C carbide are enriched in both Mo and Fe and have a fee structure with lattice parameter a=1.105 nm (Figure 1).

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