Fabrication of Gelatin/Chitosan/Zeolite Composite Packaging Film for Enhancing the Quality of Fresh Meat During Storage

Valorisation of fishery by-products for the extraction of gelatin-like biopolymers is an active area of research presently due to their significant food and biomedical applications. In the present study, gelatin was extracted from the discarded portions of the Amphioctopusmarginatus. One of the major octopus species landed near the Kerala coast. Physico-chemical and functional characterization of the extracted gelatin was done based on SDS- PAGE, UV-Vis spectrophotometer, FTIR, XRD, HPLC, gelling and emulsifying capacities. Later, biocomposite film based on the gelatin/chitosan/zeolite was developed and characterized according to its solubility, opacity, spectroscopic analysis, antioxidative and antimicrobial activities. The results showed the dimensions of improving the properties of biopolymer films in terms of its ability to prevent the oxidative lipid changes, protein degradation and microbial spoilage in packaged fresh chicken meat over 9 days of refrigerated storage. The gelatin/chitosan/zeolite composite film thus developed has the potential to serve as a promising bio-based packaging material in the food packaging and meat processing industries.

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Expression and functional characterization of a novel antimicrobial peptide: human beta-defensin118

10.21203/rs.3.rs-17411/v1 ◽

2020 ◽

Author(s):

Qian Lin ◽

KunHong Xie ◽

Daiwen Chen ◽

Bing Yu ◽

Xiangbing Mao ◽

...

Keyword(s):

Inflammatory Responses ◽

Functional Characterization ◽

Antimicrobial Activities ◽

Bacterial Strains ◽

E Coli ◽

Gram Positive Bacteria ◽

Sds Page ◽

Novel Host ◽

Late Apoptosis

Abstract Background: β-defensin 118 (DEFB118 ) is a novel host defence peptide (HDP) identified in human. To evaluate its potentials for future utilization, the DEFB118 gene was expressed in Escherichia coli ( E. coli ) and the recombinant protein was fully characterized. Methods: The DEFB118 protein was obtained by heterologous expression using E. coli Rosetta (DE3). Antibacterical activity of DEFB118 were determined by using various bacterial strains. IPEC-J cells challenged by E. coli K88 were used to determine its influences on inflammatory responses. Results: The E. coli transformants yielded more than 250 mg/mL D EFB118 protein after 4 h induction by 1.0 mM IPTG. The DEFB118 was estimated by SDS-PAGE to be 30 kDa, and MALDI-TOF analysis verified it is a human β-defensin 118. Importantly, the DEFB118 showed antimicrobial activities against both Gram-negative bacteria ( E. coli K88 and E. coli DH5α) and Gram-positive bacteria ( S. aureus and B. subtilis ), with a minimum inhibitory concentration (MIC) of 4 μg/mL. Hemolytic assays showed that DEFB118 had no detrimental impact on cell viability. Additionally, DEFB118 was found to elevate the viability of IPEC-J2 cells upon E. coli K88 challenge. Moreover, DEFB118 significantly decreased cell apoptosis in the late apoptosis phase and down-regulated the expression of inflammatory cytokines such as the IL-1β and TNF-a in the IPEC-J2 cells exposure to E. coli K88. Conclusions: These results suggested a novel function of the mammalian defensins, and the anti-bacterial and anti-inflammatory properties of DEFB118 may allow it a potential substitute for conventionally used antibiotics or drugs.

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Novel biochemical and functional insights into nuclear Ca2+ transport through IP3Rs and RyRs in osteoblasts

AJP Renal Physiology ◽

10.1152/ajprenal.2000.278.5.f784 ◽

2000 ◽

Vol 278 (5) ◽

pp. F784-F791 ◽

Cited By ~ 21

Author(s):

Olugbenga A. Adebanjo ◽

Gopa Biswas ◽

Baljit S. Moonga ◽

Hindupur K. Anandatheerthavarada ◽

Li Sun ◽

...

Keyword(s):

Gene Expression ◽

Nuclear Envelope ◽

Nuclear Membrane ◽

Ryanodine Receptors ◽

Functional Characterization ◽

Inositol Trisphosphate Receptors ◽

Nuclear Membranes ◽

Sds Page ◽

Direct Regulation

We report the first biochemical and functional characterization of inositol trisphosphate receptors (IP3Rs) and ryanodine receptors (RyRs) in the nuclear membrane of bone-forming (MC3T3-E1) osteoblasts. Intact nuclei fluoresced intensely with anti-RyR (Ab34) and anti-IP3R (Ab40) antisera in a typically peripheral nuclear membrane pattern. Isolated nuclear membranes were next subjected to SDS-PAGE and blotted with isoform-specific anti-receptor antisera, notably Ab40, anti-RyR-1, anti-RyR-2 (Ab129), and anti-RyR-3 (Ab180). Only anti-RyR-1 and Ab40 showed bands corresponding, respectively, to full-length RyR-1 (∼500 kDa) and IP3R-1 (∼250 kDa). Band intensity was reduced by just ∼20% after brief tryptic proteolysis of intact nuclei; this confirmed that isolated nuclear membranes were mostly free of endoplasmic reticular contaminants. Finally, the nucleoplasmic Ca2+ concentration ([Ca2+]np) was measured in single nuclei by using fura-dextran. The nuclear envelope was initially loaded with Ca2+ via Ca2+-ATPase activation (1 mM ATP and ∼100 nM Ca2+). Adequate Ca2+ loading was next confirmed by imaging the nuclear envelope (and nucleoplasm). Exposure of Ca2+-loaded nuclei to IP3 or cADP ribose resulted in a rapid and sustained [Ca2+]np elevation. Taken together, the results provide complementary evidence for nucleoplasmic Ca2+ influx in osteoblasts through nuclear membrane-resident IP3Rs and RyRs. Our findings may conceivably explain the direct regulation of osteoblastic gene expression by hormones that use the IP3-Ca2+pathway.

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EXPRESSION ANALYSIS OF THE COFFE (Coffea arabica L.) FRIGIDA4-like GENE (CaFRL4)

DESAFIOS Revista Interdisciplinar da Universidade Federal do Tocantins ◽

10.20873/uft.2359-3652.2018v5nespecialp204 ◽

2018 ◽

Vol 5 (Especial) ◽

pp. 204-213

Author(s):

Matheus Martins Daude ◽

André Almeida Lima ◽

Antonio Chalfun Junior ◽

Horllys Gomes Barreto

Keyword(s):

Molecular Mechanisms ◽

Functional Characterization ◽

Foreign Market ◽

Expression Levels ◽

Future Studies ◽

Coffea Arabica L ◽

Production Losses ◽

Flowering Process

ABSTRACT Coffee is one of the most economically important commodities. In Brazil, this crop is responsible for generating more than eight million jobs. In the foreign market, Brazil is the largest producer and exporter of coffee. Due to its economic importance, several studies aiming the improvement of coffee are conducted, but there are still problems related to its productivity and quality of the beverage, such as sequential flowering, which causes production losses and a low quality drink. Thus, understanding the molecular mechanisms involved in the flowering process is essential to elucidate how flowering occurs in the coffee crop. The FRI gene is one of the main genes involved in flowering, as it positively regulates the FLC gene at expression levels that inhibit flowering. Thus, the objective of this work was to identify and analyze the expression of the FRIGIDA4-like gene (FRL4) through Bioinformatics and real-time PCR (RT-qPCR). The CaFRL4 gene was identified and showed high expression levels in leaf during flowering, which corroborates with the literature. The results obtained provide the basis for future studies involving genetic transformation in model plants and coffee, permitting the functional characterization of this gene.

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RsSOS1 Responding to Salt Stress Might Be Involved in Regulating Salt Tolerance by Maintaining Na+ Homeostasis in Radish (Raphanus sativus L.)

Horticulturae ◽

10.3390/horticulturae7110458 ◽

2021 ◽

Vol 7 (11) ◽

pp. 458

Author(s):

Wanting Zhang ◽

Jingxue Li ◽

Junhui Dong ◽

Yan Wang ◽

Liang Xu ◽

...

Keyword(s):

Plasma Membrane ◽

Salt Stress ◽

Salt Tolerance ◽

Functional Characterization ◽

Vital Role ◽

Salt Stress Response ◽

Reading Frame ◽

Yield And Quality

Radish is a kind of moderately salt-sensitive vegetable. Salt stress seriously decreases the yield and quality of radish. The plasma membrane Na+/H+ antiporter protein Salt Overly Sensitive 1 (SOS1) plays a crucial role in protecting plant cells against salt stress, but the biological function of the RsSOS1 gene in radish remains to be elucidated. In this study, the RsSOS1 gene was isolated from radish genotype ‘NAU-TR17’, and contains an open reading frame of 3414 bp encoding 1137 amino acids. Phylogenetic analysis showed that RsSOS1 had a high homology with BnSOS1, and clustered together with Arabidopsis plasma membrane Na+/H+ antiporter (AtNHX7). The result of subcellular localization indicated that the RsSOS1 was localized in the plasma membrane. Furthermore, RsSOS1 was strongly induced in roots of radish under 150 mmol/L NaCl treatment, and its expression level in salt-tolerant genotypes was significantly higher than that in salt-sensitive ones. In addition, overexpression of RsSOS1 in Arabidopsis could significantly improve the salt tolerance of transgenic plants. Meanwhile, the transformation of RsSOS1△999 could rescue Na+ efflux function of AXT3 yeast. In summary, the plasma membrane Na+/H+ antiporter RsSOS1 plays a vital role in regulating salt-tolerance of radish by controlling Na+ homeostasis. These results provided useful information for further functional characterization of RsSOS1 and facilitate clarifying the molecular mechanism underlying salt stress response in radish.

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Chromatographic characterization of pyroligneous liquid obtained from carbonization of peach stone

Revista Ibero-Americana de Ciências Ambientais ◽

10.6008/cbpc2179-6858.2019.003.0018 ◽

2019 ◽

Vol 10 (3) ◽

pp. 207-218

Author(s):

Lidiane Schmalfuss Valadão ◽

Caroline Dos Santos Duarte ◽

Pedro José Sanches Filho

Keyword(s):

Industrial Process ◽

Chemical Characterization ◽

Raw Material ◽

Added Value ◽

Process Conditions ◽

Physico Chemical ◽

Color Density ◽

Agroindustrial Residue

The peach stone is considered an agroindustrial residue originating from the industrial process of peach in halves in syrup. It does not have an adequate destination, its final disposal is incorrect and may cause contamination in the environmental compartments. In this way, the burning of this raw material as biomass enables its reuse, besides adding value to the residue. Among the processes used for the application of this residue is the carbonization process, which allows to obtain co-products with higher added value, such as pyroligneous liquid, which represents a fraction of organic compounds condensed from the smoke emitted during carbonization. The quality of the liquid depends on the process conditions and the biomass used. Therefore, the objective of this study was to characterize the sample of pyrolignous liquid obtained from the carbonization of the peach stone, on an industrial scale, qualitative and semi quantitative. Preliminary characterization (pH, conductivity, color, density and contents of tar, organic matter and acidity) and a chemical characterization by gas chromatography coupled to mass spectrometry (GC-MS) were performed. The liquid presented satisfactory results for the physico-chemical evaluations. Regarding the qualitative determination, it was possible to identify 49 compounds. Highlighting the phenols, with 44.90% of the number of compounds, mainly methoxyphenols. These are compounds with significant added value and industrial importance, indicating their use as raw material in the production of polymer resins, among other purposes.

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Spatial and Seasonal Characterization of the Physico-Chemical Quality of the Water of the Tovè River in the Southern Benin (West Africa)

10.18782/2320-7051.3096 ◽

2017 ◽

Vol 5 (3) ◽

pp. 23-36

Author(s):

Gildas Djidohokpin ◽

Keyword(s):

West Africa ◽

Chemical Quality ◽

Physico Chemical ◽

Southern Benin

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Characterization of Soil Quality from some Farms of Ghatanji Taluka Region, Dist.Yavatmal (M.S.)

IRA-International Journal of Applied Sciences (ISSN 2455-4499) ◽

10.21013/jas.v3.n1.p1 ◽

2016 ◽

Vol 3 (1) ◽

Author(s):

R.R. Wankhade

Keyword(s):

Quality Parameters ◽

Exchange Capacity ◽

Free Calcium ◽

Density Maximum ◽

Water Capacity ◽

Physico Chemical ◽

Maximum Water ◽

Water Holding

<p>The quality of soil and availability of water are essential factors for the good yield of the crop. Hence it is necessary to analyze some quality parameters of the soil to determine the quality of soil. So in the present study is undertaken to determine the physico-chemical characteristics of some samples of soil from some farms of nearby villages of Ghatanji taluka region, Dist. Yavatmal.The soil characterization was carried out with respect to particle size distribution, bulk density, maximum water holding capacity, available water capacity, hydraulic conductivity, soil pH, electrical conductivity, cation exchange capacity, free calcium carbonate and organic carbon. The important observation during the study was that the parameters were varying for farm to farm of nearby villages. The overall quality of the soil in the study area is good and soil is not getting polluted as there is no industrial waste problem in the region.</p>

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Physico-Chemical, Nutritional, and Sensory Evaluation of Two New Commercial Tomato Hybrids and Their Parental Lines

Plants ◽

10.3390/plants10112480 ◽

2021 ◽

Vol 10 (11) ◽

pp. 2480

Author(s):

Zoltán Felföldi ◽

Floricuta Ranga ◽

Sonia Ancuta Socaci ◽

Anca Farcas ◽

Mariola Plazas ◽

...

Keyword(s):

F1 Hybrids ◽

Maternal Line ◽

Breeding Lines ◽

Paternal Line ◽

Physico Chemical ◽

Parental Lines ◽

Tomato Breeding ◽

Hedonic Scale

Tomato (Solanum lycopersicum) is the globally most consumed vegetable. The objective of this research was to analyze physico-chemical, nutritional and sensorial components (taste and flavor) in two new commercial hybrids (AS 300 F1 and AS 400 F1) and their four F7 parental lines. Two widely grown F1 hybrids (Precos F1 and Addalyn F1) were used as controls. The results obtained for carbohydrates (HPLC-RID) indicated that the highest values (27.82 mg/g) were recorded in the paternal line AS 10 of the new hybrid AS 400 F1. The highest values of total organic acids (HPLC-VWD) were recorded in Addalyn F1 (5.06 m/g), while the highest value of phenolic compounds (HPLC-DAD-ESI⁺) were identified in the maternal line AS 09 of the hybrid AS 400 F1 (96.3 µg/g). Intrinsic sensory values were analyzed by male and female tasters of different ages using a hedonic scale. The tasters’ perception revealed obvious taste differences between tomato genotypes. The study allowed determining genetic parameters of interest (heterosis and heterobeltosis) for the new hybrids, as well as a detailed characterization of the chemical composition and organoleptic quality of the parental breeding lines and their hybrids, which is useful in tomato breeding.

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Qualité des eaux pluviales stockées dans les citernes pour la consommation dans la commune de Toffo : cas de l’arrondissement de Damè

Journal of Applied Biosciences ◽

10.35759/jabs.154.4 ◽

2020 ◽

Vol 154 ◽

pp. 15871-15880

Author(s):

DOVONOU Edia Flavien ◽

HOUNSOU .B. Mathieu ◽

SAMBIENOU W. Gédéon ◽

ADANDEDJAN Constant ◽

HOUESSOUGA Farida ◽

...

Keyword(s):

Preliminary Treatment ◽

Household Surveys ◽

Water Sampling ◽

Safe Drinking Water ◽

Mots Clés ◽

Sampling Campaign ◽

Physico Chemical ◽

Water Borne

Objectifs : Le but visé par cette recherche est d’apprécier la qualité des eaux de pluie stockées dans les citernes à Damè afin de prévenir les pathologies sur les populations. Méthodologie et Résultats : Les enquêtes menées auprès des ménages ont été complétées par une campagne d’analyse. Vingt (20) citernes ont été ciblées et ont permis de d’apprécier la qualité bactériologique et physico-chimique des eaux stockées au vu des usages effectifs qui en sont faits. Les enquêtes menées auprès des ménages ont montré un manque d’hygiène aux alentours des citernes. Sur un total de 20 échantillons d’eau analysés, un seul échantillon est exempt de toute contamination. Conclusion et application : La consommation de ces eaux expose donc les usagers aux maladies hydriques tels que les gastro-entérites et bien d’autres pathologies. Toutefois, au regard de la difficulté d’accès de ces zones aux sources d’eau potable et grâce à l’état non trouble de ces eaux, ces dernières peuvent être consommées après un traitement préalable au chlore. Mots clés : Citerne, physico-chimique, bactériologie, risque sanitaire, Toffo Physico-chemical and bacteriological characterization of Rain water stored in tanks for consumption in the community of Toffo: case of the borough of Damè ABSTRACT Objectives: The aim of this research is to assess the quality of the rainwater stored in the cisterns in Damè in order to prevent pathologies on the populations. Methodology and Results: In addition to household surveys, a water sampling campaign was conducted. Twenty (20) tanks were analyzed to assess the bacteriological and physicochemical quality of the stored water used by the households for their various needs. Household surveys showed a lack of hygiene around the reservoirs. Out of a total of 20 samples analyzed, only one sample is free of contamination. Dovonou et al., J. Appl. Biosci. 2020 Qualité des eaux pluviales stockées dans les citernes pour la consommation dans la commune de Toffo : cas de l’arrondissement de Damè 15872 Conclusion: The consumption of these waters therefore exposes users to water-borne diseases such as gastroenteritis and many other pathologies. However, in view of the difficulties to access safe drinking water in these areas, water stored in these tanks are not turbid and could f these waters, consumed after treatment. We strongly advise a preliminary treatment with chlorine of the waters before their uses. Key words: Tank, physico-chemical, bacteriology, health risk, Toffo.

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