scholarly journals Amelioration of seed yield, oil content and oil quality through induced mutagenesis in sesame (Sesamum indicum L.)

2015 ◽  
Vol 44 (1) ◽  
pp. 15-22 ◽  
Author(s):  
Tamina Begum ◽  
Tapash Dasgupta

Thirty mutant lines selected from 3 widely adapted genotypes of sesame viz. Rama, SI 1666 and IC 21706 (ten from each of the three genotypes), developed by induced physical (?-rays) and chemical (EMS) mutagens, were evaluated against their respective control genotype for yield and its important attributes in M4 generation to reveal the ramification of mutagens for disclosing the magnitude of variation among mutants in advance generation and also to identify the promising positive mutants to refurbish new improved varieties of sesame. Mutants professing higher seed yield were evaluated for oil quantity and quality. All selected mutant lines evinced improved seed yield over their respective controls. Irrespective of the genotypes highest yield was recorded in the line induced by 0.5% EMS. Based on mean seed yield and its components, selected 10 superior mutants, also possessed high oil percentage with a better oil profile having relatively more polyunsaturated fatty acid content, specially linoleic acid, than the control, indicating potentiality of mutation breeding to restructure plants with high yield, improved oil percentage and quality. DOI: http://dx.doi.org/10.3329/bjb.v44i1.22718 Bangladesh J. Bot. 44(1): 15-22, 2015 (March)

2021 ◽  
pp. 203-208
Author(s):  
Shamsun Nahar Begum ◽  
Mirza Mofazzal Islam ◽  
Rigyan Gupta

Abstract Chickpea has a high yield potential, nutritional importance and diversity of use. A mutation breeding programme was undertaken at Bangladesh Institute of Nuclear Agriculture (BINA) with a view to developing early-maturing, large-seeded and high-yielding varieties of chickpea. Seeds of the popular chickpea variety 'Desi Binasola-2' were treated with different doses of gamma-rays (200, 300 and 400 Gy). The treated seeds were grown in batches according to dose for raising the M1 generation. M2 seeds were collected from individual M1 plants and subsequently grown in plant-progeny rows in the M2 generation and selections were made from the M2 families. Only 85 plants were selected from the M2 population and these were grown in the M3 generation. The mutant 'CPM-kabuli' and 28 other mutants were selected from M3 and were grown in the M4 generation. Only five mutants, including 'CPM-kabuli', were selected from M4 and were grown in M5. The selected mutant 'CPM-kabuli' along with check varieties were put into preliminary yield trials. Finally, the mutant lines were evaluated, with respect to two check varieties, in advanced, zonal-yield, on-farm and on-station trials in successive generations. All the selected mutant lines were grown at different locations in Bangladesh to observe the yield and other characteristics. The performance of the mutants was evaluated under two management practices: research management and farmers' management. Contrary to its parent, 'CPM-kabuli' was found to be tolerant to root rot and Botrytis grey mould, and also showed greater tolerance to pod borer insect-pest infestation than other mutants and check varieties. The main improved attributes are a cream seed coat colour, which reflects kabuli type, larger seed size and higher seed yield. The mutant 'CPM-kabuli' matures in the range of 115-125 days and is high yielding (1.7 t/ha). Considering all these, the bold Kabuli type chickpea mutant 'CPM-kabuli' was registered as the variety 'Binasola-9' for commercial cultivation during 2017 and is suitable for farmers in drought-prone areas in Bangladesh.


2020 ◽  
Vol 4 (3) ◽  
pp. 453-456
Author(s):  
Paul Madina

A field experiment was carried out in the rainy season of 2019 in Gombe states, Nigeria. The experiment was carried out in Tal, Billiri Local Government, Gombe State. The aim of the experiment was investigate the performance of ten varieties of sesame. The ten different varieties were; E-8, NCRIBEN-01, NCRIBEN-02, NCRIBEM-031, YANDEV-55, EX-BR-1, EX-BR-2, E-SUDAN, 560-1 and LOCAL. The treatments were laid in a randomized complete block design with three replications. The treatments were combined to have 10 plots in a block with 1 meter between the block and 0.5 meters within the plots. The seeds were placed in 5cm depth at the spacing of 15 x 75cm. During the research growth parameters like plant height, number of leaves and number of branches were measured. Other growth parameters like days of 1st flower, 50% flower, days of maturity, number of capsule per plant, seeds per capsule, number of capsule per leaves axis, 1000 seed weight and seed yield were also recorded. The results of the experiment revealed that sesame generally responded to both varietal and fertilizer effects. All the parameters studied have significantly (P≤0.05) responded to the varietal effects, here variety NCRIBEN-01 and E-8 were observed to perform higher in both growth parameters, yield related character and overall yield. Based on the result obtained it can be suggested that the use of E-8, NCRIBEN-01, NCRIBEN-02, NCRIBEN-031, YANDEV-55 will give high yield followed by EX-BR-1, EX-BR-2, E-SUDAN and 506-1 leading to optimum yield for farmers in Tal, Billiri Local 


Plants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 321 ◽  
Author(s):  
Ambreen Asif ◽  
Mohammad Yunus K. Ansari ◽  
Abeer Hashem ◽  
Baby Tabassum ◽  
Elsayed Fathi Abd_Allah ◽  
...  

In the present investigation, the leaf proteome profile of the macro-mutant lines of Nigella sativa L. was analyzed to identify the key proteins involved in the expression of traits associated with the morphology, seed yield, and content of thymoquinone. In our earlier study, the macro-mutants were generated with contrasting morphological traits and seed yields through induced mutagenesis, using ethyl methyl sulfonate, gamma rays, and combinations of both. Analysis of the leaf proteome of the control and macro-mutant lines of N. sativa showed that twenty-three proteins were differentially expressed. These differentially expressed proteins were sequenced through mass spectrometry and identified using the MASCOT software. On the basis of their function, these proteins were categorized into several groups. Most proteins were found in the categories of signal transduction (18%) and carbon metabolism (18%). A total of 13% of proteins belonged to the categories of energy and metabolism. Proteins in the categories of secondary plant metabolism, stress defense, cytoskeleton, and protein synthesis were also found. The polycomb group protein (FIE1), transcription factor (PRE1), and geranyl diphosphate synthase were notable proteins, in addition to some proteins of signal transduction and carbon metabolism. Expression patterns of the differentially expressed proteins were also studied at the transcript level by using qRT-PCR. Transcriptomics analysis was consistent with the proteomics data. This study shows the changes that take place at the proteomic level through induced mutagenesis, as well as the involvement of some proteins in the expression traits associated with plant height, seed yield, and the thymoquinone content of N. sativa. The identified proteins might help elucidate the metabolic pathways involved in the expression of traits, including seed yield, and the active compounds of medicinal plants.


2021 ◽  
Vol 27 (2) ◽  
Author(s):  
Shipra Raj

During the induced mutagenesis in six rowed barley, 18 mutant lines were isolated from the M2 and M3 generations. Thirteen lines were induced by gamma rays, four by EMS and one by HA. These lines were screened for resistance to stem rust and loss assessment. Mutants were grown in field as well as in earthen pots. Artificial inoculums were provided in condition suitable for rust occurrence. Rust reaction was recorded according to modified Cabb’s scale and the rust intensity was classified as 0,5, 10, 25, 40 and 65 percent. Lines K50-38, K50-97, K50-102 and K40-73 were completely free from infection (0 %) and thought to be immune lines(I). K50-47, KE-4-47 and G50-47 exhibited traces of symptoms and placed under 5% intensity and were very resistant(R). Line KE2-63 exhibited 10% intensity and classified as moderately resistant(MR). Line K50-44; K40-11, K40-67, K30-91, KE3-75, G50-35 and GH3-32 showed 25% intensity of pustules and were put into moderately susceptible(MS) class. One line K30-82 showed 40% intensity and classified as susceptible(S), while line KE4-87 exihibited 65% intensity and was classified as heavily susceptible. Class 100 percent was not observed. Immune, very resistant and moderately resistant were identified as resistant lines and selected for further study, while moderately susceptible and heavily susceptible lines were identified as susceptible lines and discarded. Reduction in yield contributing traits i.e. number of grains/spike, test weight, seed yield and biological yield was also observed which was positively correlated with disease intensity.


Author(s):  
N.V. Sergeev ◽  
◽  
A.Yu. Pivkin

The experience of cultivation of soybeans in SC "Agricultural machinery" of the Kaluga region on an area of 190 hectares shows that the soybean variety Alaska is sufficiently adapted to the soil and climatic conditions of this region, provides a high seed yield (up to 32 c / ha) and a high yield (up to 1344 kg / ha) relatively inexpensive protein. However, this variety has a long growing season (95-105 days) and therefore desiccation of crops is required for harvesting for seeds.


Diversity ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 105
Author(s):  
Amirah Yuslan ◽  
Sharifah Najuwa ◽  
Atsushi Hagiwara ◽  
Mazlan A. Ghaffar ◽  
Hidayu Suhaimi ◽  
...  

Salinity is a known factor in shaping population dynamics and community structure through direct and indirect effects on aquatic ecosystems. Salinity changes further influence food webs through competition and predation. The responses of Moina macrocopa (Cladocera) collected from Setiu Wetland lagoon (Terengganu) was evaluated through manipulative laboratory experiments to understand the ability of M. macrocopa to tolerate high salinity stress. Specifically, the fatty acid composition, growth, survival, and reproduction of this cladocerans species was examined. Sodium chloride (NaCl) as used in the treatments water with the concentration 0, 4, 6, 8, 12, and 15 salinity. Fatty acid levels were determined using Gas Chromatography and Mass Spectrophotometry (GC-MS). The results indicated that optimal conditions produced the highest fatty acid content, especially the polyunsaturated fatty acid content, such as EPA (eicosapentaenoic acid), ALA (alpha-linoleic acid), ARA (arachidonic acid), and DHA (docosahexaenoic acid). Furthermore, M. macrocopa survival was best at salinity 0, with a percentage of 98%, whereas the opposite occurred at salinity 15, with approximately 20% of viable animals surviving. Besides, M. macrocopa also showed the highest reproduction rate at salinity 0 (e.g., average initial age of reproduction, 4.33 ± 0.58 days) compared with other salinities level. Interestingly, the difference in growth at different salinities was not evident, an unusual finding when considering adverse effects such as osmoregulation pressure on the organism. Based on the results, we conclude that M. macrocopa can only tolerate salinity below salinity 8 and cannot withstand stressful environmental conditions associated with salinities above 8.


Helia ◽  
2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Khaled Mohamed Aboelkassem ◽  
Asmaa Abd-EL-Halime Ahmed ◽  
Mohamed Ali Abdelsatar

Abstract The present investigation was carried out to evaluate agronomic performance and oil quality of seven sunflower genotypes at Shandaweel Research Station, Agricultural Research Center, Sohag, Egypt during 2018 and 2019 summer seasons. These genetic materials were sown in a randomized complete block design having three replications. Significant genetic variations among evaluated sunflower genotypes for agronomic traits and oil quality were observed. The superior sunflower genotypes were Line 120 for seed yield per hectare (3102.38 kg), Sakha 53 for seed oil content (44.63 %) and Line 125 for oil quality where it contained the highest proportion of unsaturated fatty acids (89.20 %). The phenotypic coefficients of variation were slightly higher than genotypic coefficients of variation for all studied traits. High heritability (exceeded 60%) and genetic advance as percent of mean (ranged from medium to high, exceeded 10%) was observed for most studied traits. Seed yield per plant positively correlated with plant height, stem diameter, head diameter, and 100-seed weight and most chemical traits at phenotypic and genotypic levels. Maximum phenotypic direct effects on seed yield per plant were observed for 100-seed weight, head diameter and total unsaturated fatty acids. While, the highest genotypic direct effect on seed yield per plant was observed for head diameter. Hence, most studied traits could be employed as selection criteria for improving evaluated sunflower genotypes.


Lipid / Fett ◽  
1996 ◽  
Vol 98 (4) ◽  
pp. 141-144 ◽  
Author(s):  
W. De Greyt ◽  
M. Kellens ◽  
A. Huyghebaerts

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