Antioxidants protect proteins' anchorage to the bilayer by improving plasma membrane integrity of ram spermatozoa during liquid preservation in a soya lecithin-based diluent

2017 ◽  
Vol 52 (6) ◽  
pp. 1052-1060 ◽  
Author(s):  
RK Paul ◽  
D Kumar ◽  
SMK Naqvi
Keyword(s):  
Plasma Membrane ◽  
Membrane Integrity ◽  
Plasma Membrane Integrity ◽  
Soya Lecithin

scholarly journals Cryopreservation of Semen of Mithun and Siri Bulls

2014 ◽  
Vol 12 (2) ◽  
pp. 147-153 ◽  
Author(s):  
P Dorji ◽  
V Pattarajinda ◽  
T Vongprolub
Keyword(s):  
Plasma Membrane ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Plasma Membrane Integrity ◽  
Soya Lecithin ◽  
Semen Volume ◽  
Significant Difference ◽  
Semen Characteristics ◽  
The Mean ◽  
Mass Activity

The objective of the study was to assess the semen characteristics of Jersey, Mithun and Siri breeds of bulls and to compare the suitability of Tris Eggyolk Citrate and commercial soya lecithin based BIOXcell extenders for cryopreservation of semen from the these breeds of bulls. The mean semen volume was 4.55± 1.57, 4.86±1.33 and 4.83±1.72 ml in Jersey, Mithun and Siri bulls, respectively. Mean mass activity of 3, mean initial motility of 78.0±4.22, 80±4.47 and 78.33±4.08%, mean sperm concentration of 1416±484.19, 1530.91±517.41, 1122.83±293.68 x106 and mean livability of 69.20±6.81, 70.45±8.42, 76.67±8.17% was found in Jersey, Mithun and Siri bulls, respectively. There is no significant difference in all above mentioned variables among the breeds. The post-thaw motility of semen cryopreserved in Tris Eggyolk Citrate extender was found to be 45.50 ± 4.38, 46.82 ± 6.81 and 47.50 ± 8.22% in Jersey, Mithun and Siri bulls, respectively. Whereas in BIOXcell extender, it was 45.50 ± 5.99, 46.36 ± 5.05 and 49.17 ± 5.85 % in Jersey, Mithun and Siri bulls, respectively. Similarly, the plasma membrane integrity of thawed spermatozoa in Tris eggyolk extender was 17.50±2.32, 21.00±3.12 and 21.58±3.83% in Jersey, Mithun and Siri bulls, respectively. Whereas in BIOXcell extender, it was found to be 20.45±2.86, 20.86±2.281 and 22.33±3.24% in Jersey, Mithun and Siri bulls, respectively. No significant difference (p>0.05) was observed in post-thaw motility as well as in plasma membrane integrity between extenders and between breeds, and there was no significant effect (p>0.05) of breed x extender interaction. It can be concluded that the semen characteristics of Siri and Mithun bulls fall with the range established for other breeds of bulls. Furthermore, both Tris eggyolk and BIOXcell extenders are equally suitable for the cryopreservation of semen from these three breeds of Bulls.DOI: http://dx.doi.org/10.3329/bjvm.v12i2.21277 Bangl. J. Vet. Med. (2014). 12 (2): 143-153 

Comparision of DNA fragmentantion and plasma membrane integrity between chilled and frozen semen of bulls

2014 ◽  
Author(s):  
Mello Papa Patricia de ◽  
Carlos Ramires Neto ◽  
Priscilla Nascimento Guasti ◽  
Rosiara Rosaria Dias Maziero ◽  
Yame F R Sancler-Silva ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Integrity ◽  
Plasma Membrane Integrity ◽  
Frozen Semen

scholarly journals Plasma membrane integrity in health and disease: significance and therapeutic potential

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Catarina Dias ◽  
Jesper Nylandsted
Keyword(s):  
Plasma Membrane ◽  
Therapeutic Potential ◽  
Calcium Influx ◽  
Membrane Integrity ◽  
Cell Types ◽  
Physical Parameters ◽  
Membrane Repair ◽  
Plasma Membrane Integrity ◽  
Repair Mechanisms ◽  
And Function

AbstractMaintenance of plasma membrane integrity is essential for normal cell viability and function. Thus, robust membrane repair mechanisms have evolved to counteract the eminent threat of a torn plasma membrane. Different repair mechanisms and the bio-physical parameters required for efficient repair are now emerging from different research groups. However, less is known about when these mechanisms come into play. This review focuses on the existence of membrane disruptions and repair mechanisms in both physiological and pathological conditions, and across multiple cell types, albeit to different degrees. Fundamentally, irrespective of the source of membrane disruption, aberrant calcium influx is the common stimulus that activates the membrane repair response. Inadequate repair responses can tip the balance between physiology and pathology, highlighting the significance of plasma membrane integrity. For example, an over-activated repair response can promote cancer invasion, while the inability to efficiently repair membrane can drive neurodegeneration and muscular dystrophies. The interdisciplinary view explored here emphasises the widespread potential of targeting plasma membrane repair mechanisms for therapeutic purposes.

scholarly journals Plasma membrane integrity: implications for health and disease

BMC Biology ◽  
2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Dustin A. Ammendolia ◽  
William M. Bement ◽  
John H. Brumell
Keyword(s):  
Plasma Membrane ◽  
Membrane Damage ◽  
Membrane Integrity ◽  
Whole Body ◽  
Plasma Membrane Integrity ◽  
Plasma Membrane Damage ◽  
Cellular Environment ◽  
Health And Disease ◽  
Repair Pathways ◽  
The Impact

AbstractPlasma membrane integrity is essential for cellular homeostasis. In vivo, cells experience plasma membrane damage from a multitude of stressors in the extra- and intra-cellular environment. To avoid lethal consequences, cells are equipped with repair pathways to restore membrane integrity. Here, we assess plasma membrane damage and repair from a whole-body perspective. We highlight the role of tissue-specific stressors in health and disease and examine membrane repair pathways across diverse cell types. Furthermore, we outline the impact of genetic and environmental factors on plasma membrane integrity and how these contribute to disease pathogenesis in different tissues.

scholarly journals Arabidopsis Synaptotagmin1 Maintains Plasma Membrane Integrity

2008 ◽  
Vol 20 (12) ◽  
pp. 3182-3182 ◽  
Author(s):  
Nancy A. Eckardt
Keyword(s):  
Plasma Membrane ◽  
Membrane Integrity ◽  
Plasma Membrane Integrity

Assessment of the Functional Integrity of Hepatocytes: A Brief Review

1993 ◽  
Vol 21 (3) ◽  
pp. 324-329
Author(s):  
Jeffrey R. Fry ◽  
Alison H. Hammond
Keyword(s):  
Plasma Membrane ◽  
Trypan Blue ◽  
Membrane Integrity ◽  
Blue Dye ◽  
Plasma Membrane Integrity ◽  
Functional Integrity ◽  
Cellular Integrity

A variety of approaches to assessment of cellular integrity exist, based on tests of integrity of the plasma membrane, tests of metabolic competence, and asessment of morphology. By definition, such approaches address different aspects of cellular integrity and hence are not interchangeable indices of cellular integrity. Accordingly, it would be most appropriate to characterise hepatocyte preparations on the basis of more than just trypan blue dye exclusion (a test of plasma membrane integrity) as is customary. A scheme for the choice of the most appropriate mix of tests of cellular integrity is presented.

Toxic and osmotic effects of glycerol on human granulocytes

1984 ◽  
Vol 247 (5) ◽  
pp. C382-C389 ◽  
Author(s):  
W. J. Armitage ◽  
P. Mazur
Keyword(s):  
Plasma Membrane ◽  
Osmotic Stress ◽  
Cell Volume ◽  
Membrane Integrity ◽  
Fluorescein Diacetate ◽  
Major Fraction ◽  
Plasma Membrane Integrity ◽  
Human Granulocytes ◽  
Osmotic Effects

Human granulocytes are damaged by exposure to concentrations of glycerol as low as 0.5 M. We therefore investigated the addition of glycerol to granulocytes and its subsequent dilution under various conditions to try to distinguish between toxic and harmful osmotic effects of glycerol. The lesion caused by glycerol at 0 degree C was expressed as a loss of plasma membrane integrity (as visualized by fluorescein diacetate) only after incubation (greater than or equal to 1 h) at 37 degrees C. This damage was not ameliorated when osmotic stress was lessened by reducing the rates of addition and dilution of glycerol to keep the computed cell volume within 80-170% of isotonic cell volume. However, when osmotic stress was reduced further by increasing the temperature of addition and dilution of glycerol from 0 degree C to 22 degrees C, the tolerance of the cells to 1 M glycerol increased somewhat. Reducing exposure to glycerol to 3 min or less at 0 degree C greatly increased survival, but this time was too short to allow glycerol to equilibrate intracellularly. Finally, the presence of extra impermeant solute (NaCl or sucrose) in the medium to reduce the equilibrium cell volume to 60% of isotonic cell volume enabled granulocytes to survive 30-min exposure to 1 M glycerol at 0 degree C, but cells had to remain shrunken during the 37 degrees C incubation to prevent the loss of membrane integrity. Suspensions that contained damaged granulocytes formed aggregates when incubated at 37 degrees C, and these aggregates were responsible for a major fraction of the observed loss in viability.

scholarly journals Effects of high ambient temperature on fish sperm plasma membrane integrity and mitochondrial activity — A flow cytometric study

2016 ◽  
Vol 67 (2) ◽  
pp. 125-132 ◽  
Author(s):  
Szabolcs Tamás Nagy ◽  
Balázs Kakasi ◽  
László Pál ◽  
Máté Havasi ◽  
Miklós Bercsényi ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Ambient Temperature ◽  
Membrane Integrity ◽  
High Ambient Temperature ◽  
Mitochondrial Activity ◽  
Plasma Membrane Integrity ◽  
Flow Cytometric ◽  
Fish Sperm ◽  
Sperm Plasma Membrane ◽  
Flow Cytometric Study

scholarly journals Efek Penambahan Vitamin E dalam Pengencer Glukosa Fosfat terhadap Daya Tahan Hidup Spermatozoa Domba pada Suhu 5 °C

2017 ◽  
Vol 9 (1) ◽  
pp. 25
Author(s):  
Nilawati Widjaya
Keyword(s):  
Plasma Membrane ◽  
Vitamin E ◽  
Analysis Of Variance ◽  
Membrane Integrity ◽  
Plasma Membrane Integrity ◽  
Glucose Phosphate ◽  
Randomized Design ◽  
Completely Randomized Design

<p class="p1">The purpose of this study was to know the effect of vitamin E addition in glucose phosphate diluent on the survival of sheep spermatozoa at 5 °C. The xperiment used 3 rams. The design used was Completely Randomized Design (CRD) with 5 treatments and 4 replicates, each replicate with 3 ejaculate cement of sheep. The treatments were the dose of Vitamin E with E<span class="s1">0 </span>= 0 μg/ml, E<span class="s1">1 </span>= 1 μg/ml, E<span class="s1">2 </span>= 4 μg/ml, E<span class="s1">3 </span>= 7 μg/ml, and E<span class="s1">4 </span>= 10 μg/ml. Variables measured were motility, live spermatozoa and plasma membrane integrity. Data were analyzed by using analysis of variance according to Steel and Torrie (1981). The result showed that treatment of vitamin E with a dose of 1 μg/ml - 10 μg/ml l had no significant effect on motility, percentage live spermatozoa and membrane integrity of sheep spermatozoa on day two (P&gt; 0.05).</p>

Sign in / Sign up

Export Citation Format

Share Document