scholarly journals Antibiotic sensitivity pattern of diarrhoeal pathogens in under five children

2020 ◽  
Vol 52 (1-2) ◽  
pp. 35-39
Author(s):  
Mst Khashruba Pervin ◽  
Sanya Tahmina Jhora ◽  
Shikha Paul ◽  
Akhtarun Naher
Keyword(s):  
Escherichia Coli ◽  
Membrane Filter ◽  
Enteric Bacteria ◽  
Salmonella Spp ◽  
Medical College ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern ◽  
Shigella Spp ◽  
Agar Media ◽  
Campylobacter Spp

Background: Different bacterial enteropathogens are responsible for diarrhoea in children less than 5 years of age in Bangladesh. This study aimed to determine the antimicrobial sensitivity pattern of diarrhoeal pathogens that is necessary to know for the specific management of acute bacterial diarrhoea. Objectives: To find out the bacterial enteropathogens causing diarrhoea and their antimicrobial sensitivity pattern in under 5 children. Methodology: This was an observational cross sectional study carried out at Microbiology Department of Sir Salimullah Medical College, Dhaka. A total of 217 diarrhoeal patients 0-5 years age, attending Pediatric unit of Sir Salimullah Medical College and Mitford Hospital. Dhaka and Dhaka Shishu Hospital, Dhaka during the period of July, 2015 to June, 2016, were enrolled in the study. For isolation of the common enteric bacteria, diarrhoeal stools were cultured on MacConkey agar media and for Campylobacter spp, diarrhoeal stools were cultured on selective Modified Campy BAP media and nonselective Blood agar media with membrane filter. Positive culture was confirmed by Gram stain morphology and standard biochemical tests. Antimicrobial susceptibility test were used to identify the bacterial sensitivity pattern in the isolates. Results: Out of 217 diarrhoeal stools, bacteria was isolated from 97 (44.70%) patients by culture. Among 97 culture positive cases Eschericha coli (51- 52.58%), Campylobacter spp. (23-23.71%), Shigella spp. (15-15.46%) and Salmonella spp. (8-8.25%) were isolated. Mecillinam is the most sensitive drug for Escherichia coli, Campylobacter spp., Shigella spp. and Salmonella spp. Among the other antimicrobials, Campylabacter spp. were sensitive to azithromycin, erythromycin and nitrofurantoin. Escherichia coli were sensitive to nitrofurantoin, gentamicin and azithromycin. Shigella spp. were sensitive to ceftriaxone, ciprofloxacin and nitrofurantoin and Salmonella spp. were sensitive to ceftriaxone, cotrimoxazole and gentamicin. Conclusion: Mecillinam and nitrofurantoin were highly effective against bacteria causing diarrhoea in children. Bang Med J (Khulna) 2019; 52 : 35-39

scholarly journals Causative agents for diarrhoea in under 5 children in a tertiary care hospital

2019 ◽  
Vol 51 (1-2) ◽  
pp. 25-28
Author(s):  
Mst Khashruba Pervin ◽  
Sanya Tahmina Jhora ◽  
Shikha Paul ◽  
Akhtarun Naher ◽  
Dipika Sarkar
Keyword(s):  
Escherichia Coli ◽  
Tertiary Care ◽  
Public Health Problem ◽  
Enteric Bacteria ◽  
Giardia Intestinalis ◽  
Major Public Health Problem ◽  
Care Hospital ◽  
Medical College ◽  
Agar Media ◽  
Campylobacter Spp

Background: nfectious diarrhoea is a major public health problem in developing countries like Bangladesh. Bacteria and parasites are important etiological agents for diarrhoea in infants and children. Objectives: To isolate and identify the causative organisms of diarrhoea from diarrhoeal stools in under 5 children. Methodology: This was an observational cross sectional study carried out at Microbiology Department of Sir Salimullah Medical College, Dhaka. A total of 217 diarrhoeal patients upto 5 years age, attending Pediatric unit of Sir Salimullah Medical College and Mitford Hospital, Dhaka and Dhaka Shishu Hospital, Dhaka during the period of July 2015 to June 2016, were enrolled in the study. For isolation of enteric bacteria, diarrhoeal stools were inoculated on Mackonkey agar media and for Campylobacter spp. diarrhoeal stools were inoculated on selective Modified Campy-BAP media and nonselective Blood agar media with membrane filter. Positive culture was confirmed by Gram stain morphology and standard biochemical tests. Oocysts of Cryptosporidium were identified by Modified Ziehl Neelsen staining and other parasites were identified by wet mount preparation. Results: Out of 217 diarrhoeal stools bacteria were isolated from 97 (44.7%) patients. Among 97 cases, Escherichia coli were detected in 51(52.58%) patients; Campylobacter spp. were detected in 23(23.71%) patients; Shigella spp. were detected in 15(15.46%) patients and Salmonella spp. were detected in 8(8.25%) patients. Microscopic finding of diarrhoeal stools were detected by pus cells with or without RBCs in 90(70.31%) samples. Cysts of Giardia intestinalis were found in 9(4.14%) samples, cysts of Entamoeba histolytica in 2(0.9%) samples, eggs of Trituris trichiura in 2(0.9%) samples and oocysts of Cryptosporidium were found in.5(2.30%) samples. Conclusion: Escherichia coli was the most common bacteria and Giardia intestinalis was predominant parasite causing diarrhoea in under 5 children. Bang Med J (Khulna) 2018; 51 : 25-28

Microbiological Quality of Retail Imported Unprepared Whole Lettuces: A PHLS Food Working Group Study

1999 ◽  
Vol 62 (4) ◽  
pp. 325-328 ◽  
Author(s):  
C. LITTLE ◽  
D. ROBERTS ◽  
E. YOUNGS ◽  
J. de LOUVOIS
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Microbiological Quality ◽  
Escherichia Coli O157 ◽  
Salmonella Spp ◽  
Shigella Spp ◽  
Natural Microflora ◽  
Production Practices ◽  
Campylobacter Spp

A study of imported unprepared whole lettuces sampled from supermarkets, greengrocers, shops, and market stalls found that all were of acceptable microbiological quality. Twenty-seven out of 151 (18%) imported lettuce samples had Enterobacteriaceae levels of 104 CFU/g or more. However, these bacteria that constitute part of the natural microflora of unprepared vegetables may also be derived from the soil and/or by poor handling. The pathogens, Salmonella spp., Shigella spp., Campylobacter spp., Escherichia coli O157:H7, Vibrio cholerae, Listeria monocytogenes, and also Escherichia coli, an indicator of fecal contamination, were not detected in any imported lettuces, indicating that hygiene, harvesting, and production practices were good. Imported lettuces with Enterobacteriaceae levels of 104 CFU/g or more varied with type of retail premises and the temperature at which the lettuces were displayed. Samples from greengrocers, shops, and market stalls were more likely to contain Enterobacteriaceae at levels in excess of 104 CFU/g than those from supermarkets.

scholarly journals Etiology of acute diarrhea in children in Shanghai, 2015-2018

2019 ◽  
Author(s):  
Hailing Chang ◽  
Jiayin Guo ◽  
Zhongqiu Wei ◽  
Zheng Huang ◽  
Chuning Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Foodborne Pathogens ◽  
Acute Diarrhea ◽  
Enteric Bacteria ◽  
Enterohemorrhagic Escherichia Coli ◽  
Enteric Pathogens ◽  
Enterotoxigenic Escherichia Coli ◽  
Salmonella Spp ◽  
Shigella Spp ◽  
Fresh Stool

Abstract Background Diarrhea is still a major cause of childhood morbidity and mortality worldwide. This hospital-based study aimed to monitor the consecutive epidemiological trend of etiology in children with acute diarrhea in Shanghai.Methods Outpatient children with diarrhea were prospectively enrolled within 7 days after onset of diarrheal symptoms during 2015-2018. Fresh stool samples were collected for testing enteropathogens. Enteric bacteria were identified and typed through culture and serotyping. Enteric viruses were identified through real-time PCR assay. hadResults Enteric pathogens were identified in 1572 (58.4%) of the 2692 enrolled children with acute diarrhea. Viruses were more frequently detected than bacteria (41.3% versus 25.0%), and co-infection with 2 or more pathogens was found in 13% of outpatients. Nontyphoidal Salmonella spp . (NTS) was the most common bacteria with 10.3% of isolate rate, followed by enteropathogenic Escherichia coli (EPEC) (6.5%), enteroaggregative Escherichia coli (EAEC) (6.2%), Campylobacter spp . (3.6%), enterotoxigenic Escherichia coli (ETEC) (1.1%), Shigella spp . (0.2%), and enterohemorrhagic Escherichia coli (EHEC) (0.1%). Rotavirus was the most common virus with 16.0% of detection rate, followed by norovirus (15.5%), adenovirus (7.2%), sapovirus (3.0%) and astrovirus (2.7%).Conclusions Infectious diarrhea remains the major cause of diarrhea in children in Shanghai. Rotavirus, norovirus and NTS were the major enteric pathogens responsible for diarrhea in Shanghainese children. Improving uptake of rotavirus vaccine and strengthening prevention of foodborne pathogens will be helpful to reduce the burden of diarrheal diseases in children in Shanghai.

scholarly journals Etiology of acute diarrhea among children in Ribeirão Preto-SP, Brazil

2001 ◽  
Vol 43 (1) ◽  
pp. 21-24 ◽  
Author(s):  
Marta Inês Cazentini MEDEIROS ◽  
Suzel Nogueira NEME ◽  
Paulo da SILVA ◽  
Divani Maria CAPUANO ◽  
Maria Clarice ERRERA ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Acute Diarrhea ◽  
Acute Gastroenteritis ◽  
Salmonella Spp ◽  
Standard Methods ◽  
E Coli ◽  
Cryptosporidium Spp ◽  
Shigella Spp ◽  
Campylobacter Spp

To study the main enteropathogens causing diarrhea in the region of Ribeirão Preto regarding serogroups and serotypes, the feces of 1836 children under 10 years old, from both sexes, attack of acute gastroenteritis, were analysed during a period of 4 years in Adolfo Lutz Institute - Ribeirão Preto, SP. The pathogens identified by standard methods were the following: Escherichia coli, Salmonella spp., Shigella spp., Campylobacter spp., Yersinia spp., and Cryptosporidium spp. Positive samples were 22.8% (419) with 1.7% association of pathogens. Larger isolates were mainly from children 0 to 11 months old. Enteropathogenic E. coli (EPEC) was most frequent (8.7%) with predominance of serogroup O119 (40.2%), followed by Shigella (6.2%), 63.6% of which S. sonnei.

scholarly journals In Vitro Antimicrobial Sensitivity of Escherichia coli Isolated from Clinical Sources

1970 ◽  
Vol 34 (1) ◽  
pp. 99-101
Author(s):  
Mohammad Shahriar ◽  
Mahboob Hossain ◽  
Shaila Kabir
Keyword(s):  
Escherichia Coli ◽  
Nalidixic Acid ◽  
E Coli ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern ◽  
Low Sensitivity ◽  
Academy Of Sciences ◽  
Higher Sensitivity

A study of antimicrobial sensitivity of Escherichia coli (E. coli) isolated from clinical sourcesof different diagnostic centers of Dhaka, Bangladesh was carried out to facilitate the choice of drugin the management of E. coli induced symptoms. Very low sensitivity of E. coli towards ampicillin(4%), aztreonam (4%), cloxacillin (5%), nalidixic acid (5%), ciprofloxacin (7.5%), ceftriaxone(12.5%), doxycycline (12.5%), ceftazidime (16.25%), co-trimoxazole (20%), chloramphenicol(22.51%), tetracycline (25%), and netilmicin (35%) was observed. Higher sensitivity pattern wasobserved for gentamicin (56%) and only imipenem (95%) showed sensitivity pattern possiblysusceptible enough to consider for the management of E. coli induced cases in the area under study.The low sensitivity to different antimicrobial could be attributed to their prevailing usage and abusein the area under study.Key words: E. coli; Antimicrobial sensitivity; Clinical isolatesDOI: 10.3329/jbas.v34i1.5497Journal of Bangladesh Academy of Sciences, Vol.34, No.1, 99-101, 2010

scholarly journals Antimicrobial susceptibility pattern for Salmonella Typhi isolated from blood in Shaheed Suhrawardy Medical College, Dhaka

2019 ◽  
Vol 10 (2) ◽  
pp. 96-98 ◽  
Author(s):  
Mst Naznin Tarana ◽  
Samshad Jahan Shumu ◽  
Rashida Akter Khanam ◽  
Hosne Jahan ◽  
Soma Sarker ◽  
...  
Keyword(s):  
Public Health ◽  
Typhoid Fever ◽  
Salmonella Typhi ◽  
Health Concern ◽  
Public Health Concern ◽  
Salmonella Spp ◽  
Sensitivity Testing ◽  
Female Ratio ◽  
Medical College ◽  
Sensitivity Pattern

Background: Typhoid fever remains a public health concern in developing countries. Antibiotic therapy constitutes the mainstay of management and multidrug resistant Salmonella spp has been emerged as a major public health concern. Objective: This study was done to evaluate antimicrobial sensitivity pattern of Salmonella typhi isolated from blood in Shaheed Suhrawardy Medical College Hospital. Methods: The retrospective study was done from January 2017 to December 2017 at microbiology laboratory, Shaheed Suhrawardy Medical College. A total of 367 samples, 30 isolates of Salmonella typhi obtained from blood culture. Both the indoor and outdoor patients were enrolled in this study. Results: During one year study period, total 367 cases were enrolled and the prevalence of Salmonella typhi was 30 (8.2 %). Among them (56.1%) were male with a male to female ratio 1.27:1. The bulk (50.1%) cases were in the age group of 15-30 years, 14.4% cases were in less than 15 years and 6.5% cases were in more than 60 years of age. Regarding antibiotic sensitivity pattern, 70% strains were sensitive to amikacin, 73.33% to azithromycin, 63.33% to ceftazidime, 66.66% to ceftriaxone, 86.66% to ciprofloxacin and 70% were nalidixic acid resistant Salmonella typhi. Conclusion: Ciprofloxacin may be used to treat typhoid fever cases and Ceftriaxone, azithromycin may be used as alternative drugs if they are found susceptible in culture and sensitivity testing. J Shaheed Suhrawardy Med Coll, December 2018, Vol.10(2); 96-98

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