scholarly journals Chemical and Biological Investigations of Justicia gendarussa (Burm. f)

2012 ◽  
Vol 10 (1) ◽  
pp. 53-57
Author(s):  
Md Rakib Uddin ◽  
Suchana Sinha ◽  
Md Aslam Hossain ◽  
Md Abul Kaisar ◽  
Md Khalid Hossain ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Diffusion Method ◽  
Cytotoxic Activities ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Whole Plant ◽  
Ic50 Value ◽  
Crude Methanol Extract

In this study, three compounds, stigmasterol (1), lupeol (2), 16-hydroxylupeol (3) were isolated from the petroleum ether soluble fraction of a methanol extract of whole plant of Justica gendarussa (Burm. f). The crude methanol extract as well as its petroleum ether, carbon tetrachloride and chloroform soluble Kupchan fractions were studied for antioxidant, antimicrobial and cytotoxic activities. Among the different fractions tested for antioxidant activity, the chloroform soluble partitionate was the most potent with IC50 value of 18.80 μg/ml as compared to tertbutyl- 1-hydroxytoluene (IC50=17.69 μg/ml). Antimicrobial screening of the different extractives was conducted by the disc diffusion method and all the fractions except chloroform soluble partitionate exhibited mild antimicrobial activity with zone of inhibition ranging from 7-10 mm. In brine shrimp lethality bioassay, the petroleum ether soluble materials demonstrated the highest toxicity with LC50 of 1.27μg /ml. This is the first report of isolation of compounds from J. gendarussa, and its antioxidant, antimicrobial and cytotoxic properties. DOI: http://dx.doi.org/10.3329/dujps.v10i1.10016 DUJPS 2011; 10(1): 53-57

scholarly journals Antioxidant, Antimicrobial and Cytotoxic Activities of Desmodium Motorium Merr.

2012 ◽  
Vol 5 (1) ◽  
pp. 201-205
Author(s):  
M. A. Alam ◽  
T. K. Pal ◽  
M. A. Chowdhury ◽  
M. A. Rashid ◽  
M. S. Rahaman
Keyword(s):  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Microbial Growth ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Diffusion Method ◽  
Cytotoxic Activities ◽  
Disc Diffusion Method ◽  
Whole Plant ◽  
Standard Agent

The n-hexane, carbon tetrachloride, dichloromethane soluble fractions of methanol extract of the whole plant of Desmodium motorium were subjected to antioxidant activity by indicated method, inhibition of microbial growth by disc diffusion method and cytotoxicity by brine shrimp lethality bioassay. The methanol i.e., crude extract showed highest antioxidant activity having IC50 9.5 ?g/ml as compared to 9.0 ?g/ml for standard agent BHT. The n-hexane, carbon tetrachloride and dichloromethane soluble fraction showed moderate inhibitory activity to microbial growth, while all the extractive exhibited strong cytotoxic property with the carbon tetrachloride soluble materials revealed the strongest cytotoxicity with LC50 1.54 ?g/ml.© 2013 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi: http://dx.doi.org/10.3329/jsr.v5i1.10955         J. Sci. Res. 5 (1), 201-205 (2013)

scholarly journals EVALUATION OF ANTIBACTERIAL ACTIVITY OF A CYANOBACTERIUM SCYTONEMA SCHMIDTII GOM

2021 ◽  
pp. 77-79
Author(s):  
Janardhan Namdeo Nehul
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Axenic Culture ◽  
Soil Samples ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Air Blower

Scytonema schmidtii,a cyanobacterium was isolated from the collected soil samples from different locations of Ahmednagar district of Maharashtra state (India). Identification was carried out using morphological variation and taxonomical approaches according to Desikachary (1959) and Prescott (1962) .The axenic culture of Scytonema schmidtii was obtained by using the method recommended by Bolch and Blackburn (1996). The isolated Scytonema schmidtii was grown autotropically in BG-11 medium as described by Rippka et al.,(1979) and incubated at 30±2°c.After 25 days, biomass was harvested by filtration through double layered muslin cloth and dried using air blower. The biomass of this Scytonema schmidtii species was used for the assessment of antibacterial activity against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Micrococcus flavus and Proteus mirabilis. The antibacterial activity was studied by disc diffusion method (Anonymous,1996).Methanol extract of Scytonema schmidtii showed the activity against all the tested bacterial strains.Maximum zone of inhibition (23±1.9 mm) was recorded with methanol extract of Scytonema schmidtii.

scholarly journals In vitro Antioxidant, Cytotoxic and Membrane Stabilizing Activities of Bauhinia acuminata L.

2015 ◽  
Vol 17 (1) ◽  
pp. 99-101
Author(s):  
Mohammad Firoz Khan ◽  
Rabeya Islam Shilpi ◽  
Ridwan Bin Rashid ◽  
Mohammad A Rashid
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Pharmaceutical Journal ◽  
The Other ◽  
Hypotonic Solution ◽  
Lc50 Value ◽  
Ic50 Value ◽  
Crude Methanol Extract

The crude methanol extract of leaves of Bauhinia acuminata and its Kupchan fractions were screened for antioxidant, cytotoxic and membrane stabilizing activities. Among all partitionates the aqueous soluble fraction of B. acuminata demonstrated the highest antioxidant activity with IC50 value of 7.22±0.200 ?g/ml. Moreover, the carbon tetrachloride soluble fraction showed significant cytotoxic activity having LC50 value of 12.13±0.215 ?g/ml. On the other hand, in hypotonic solution- and heat- induced conditions, the crude methanol extract inhibited haemolysis of human erythrocyte by 63.94±0.14% and 51.95±0.20%, respectively as compared to 81.97±0.77% and 42.11±0.39% demonstrated by the standard acetyl salicylic acid. DOI: http://dx.doi.org/10.3329/bpj.v17i1.22324 Bangladesh Pharmaceutical Journal 17(1): 99-101, 2014

scholarly journals Antimicrobial and Cytotoxic Activities of the Extracts of Glochidion multiloculare

2013 ◽  
Vol 10 (1) ◽  
pp. 1-11
Author(s):  
Selina Kabir ◽  
AM Sarwaruddin Chowdhury ◽  
Mohammad A Rashid ◽  
Choudhury M Hasan
Keyword(s):  
Escherichia Coli ◽  
Petroleum Ether ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Stem Bark ◽  
Diffusion Method ◽  
Cytotoxic Activities ◽  
Chloroform Fraction ◽  
Brine Shrimp Lethality ◽  
Ether Fraction

The current study was designed to investigate the antimicrobial and cytotoxic activities of methanol extract (MEGM), petroleum ether fraction (PEFGM), carbontetrachloride fraction (CTFGM), chloroform fraction (CFFGM) and aquous fraction (AQFGM) of stem bark of Glochidion multiloculare (Euphorbiaceae). Antimicrobial activity was evaluated by disc diffusion method and cytotoxic activity by brine shrimp lethality bioassay. In case of antimicrobial screening the CFFGM showed moderate inhibitory activity against B. subtilis having the zone size 12 mm and against Escherichia coli and Escherichia coli were 11 mm, while in the brine shrimp lethality bioassay, the petroleum ether soluble fraction revealed the highest cytotoxicity having LC50 of 3.11 g/ml. DOI: http://dx.doi.org/10.3329/jsf.v10i1.16273 J Sci Foundation, January-June 2012;10(1):1-11

scholarly journals Preliminary Anti-bacterial Activity of Some Indigenous Plants of Bangladesh

1970 ◽  
Vol 8 (1) ◽  
pp. 61-65 ◽  
Author(s):  
Hasina Yasmin ◽  
Md Abu Kaiser ◽  
Md Moklesur Rahman Sarker ◽  
Mohammed Shafikur Rahman ◽  
Mohammad A Rashid
Keyword(s):  
Antibacterial Activity ◽  
Plant Species ◽  
Soluble Fraction ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Zone Of Inhibition ◽  
Indigenous Plants ◽  
Shigella Boydii ◽  
Whole Plant ◽  
Indigenous Plant

The antibacterial activity of petroleum ether, carbon tetrachloride and chloroform solublefractions of crude methanol extracts of nine indigenous plant species of Bangladesh was evaluated by the agardiffusion method. Kanamycin (30 μg/disc) was used as a standard antibacterial agent. The results indicated thatall the nine plant species (not all partitionates) exhibited moderate to potent antibacterial activity against a widevariety of gram- positive and gram-negative bacteria at a concentration of 400μg/disc. Among them the carbontetrachloride soluble fraction of whole plant extract of Corriandrum sativum (family-Apiaceae) revealed thehighest antibacterial activity against Shigella boydii with zone of inhibition of 29 mm.Key words: Antibacterial activity; Disc diffusion method; Extract; Indigenous plants of Bangladesh.DOI: 10.3329/dujps.v8i1.5337Dhaka Univ. J. Pharm. Sci. 8(1): 61-65, 2009 (June)

scholarly journals Cytotoxic, thrombolytic, membrane stabilizing and anti-oxidant activities of Hygrophila schulli

2015 ◽  
Vol 10 (3) ◽  
pp. 692
Author(s):  
Md. Abu Sufian ◽  
Mohammad Rashedul Haque
Keyword(s):  
Petroleum Ether ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Soluble Extract ◽  
Vincristine Sulfate ◽  
Thrombolytic Activity ◽  
Activity Test ◽  
Anti Oxidant ◽  
Crude Methanol Extract

<p class="Abstract">The crude methanol extract of Hygrophila schulli and its petroleum ether, chloroform, ethyl acetate and aqueous soluble Kupchan partitionates were investigated for in vitro cytotoxic, thrombolytic, membrane stabilizing and anti-oxidant activities. In the brine shrimp lethality bioassay, the petroleum ether soluble extract of H. schulli showed significant cytotoxicity (LC<sub>50</sub> = 0.1 µg/mL and LC<sub>90</sub> = 15 µg/mL) as compared to vincristine sulfate (LC<sub>50</sub> = 0.4 µg/mL and LC<sub>90</sub> = 9 µg/mL). Among all the partitionates, chloroform soluble fraction demonstrated the highest thrombolytic activity with 10.5% clot lyses. Moreover, in hypotonic- and heat-induced conditions, the chloroform soluble extractive inhibited hemolysis of human erythrocyte by 113.7% and 14.3%, respectively as compared to 71.9% and 42.2% demonstrated by standard acetylsalicylic acid. On the other hand, in anti-oxidant activity test, chloroform soluble fraction revealed mild antioxidant activity (IC<sub>50</sub> = 195.1 µg/mL) as compared to standard tert-butyl-1-hydroxytoluene (IC<sub>50</sub> = 27.5 µg/mL).</p><p> </p>

scholarly journals Phytochemical and Biological Investigations of Ixora lutea Hutch.

1970 ◽  
Vol 8 (1) ◽  
pp. 17-21 ◽  
Author(s):  
Shapna Sultana ◽  
Mohammad S Rahman ◽  
Md Aslam Hossain ◽  
Md Khalid Hossain ◽  
Mohammad A Rashid
Keyword(s):  
Antioxidant Activity ◽  
Petroleum Ether ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Preliminary Screening ◽  
Brine Shrimp Lethality ◽  
Growth Inhibitory ◽  
Growth Inhibitory Activity ◽  
Ic50 Value

Two triterpenoids, lupeol (1) and stigmasterol were isolated from the petroleum ether soluble fractionof the methanolic extract of Ixora lutea Hutch. In our preliminary screening, the petroleum ether, carbontetrachloride, chloroform soluble fractions of methanol extract of the leaves and stem were subjected to antioxidant,antimicrobial and brine shrimp lethality bioassay. All of the fractions showed moderate to potent antioxidant activity,of which the chloroform soluble fraction demonstrated the strongest antioxidant activity with the IC50 value of 3.0μg/ml. In case of antimicrobial screening, all extractives showed mild growth inhibitory activity. However, in thebrine shrimp lethality bioassay, the petroleum ether soluble fraction was found to be most cytotoxic among thepartitionates having LC50 value 0.938 μg/ml.Key words: Ixora lutea; rubiaceae; lupeol; stigmasterol; antioxidant activity; brine shrimp lethality bioassay;antimicrobial.DOI: 10.3329/dujps.v8i1.5331Dhaka Univ. J. Pharm. Sci. 8(1): 17-21, 2009 (June)

Antibacterial Activity of Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Kyoung- Sun Seo ◽  
Seong Woo Jin ◽  
Seongkyu Choi ◽  
Kyeong Won Yun
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
The Other ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

scholarly journals Prenylated Flavonoids and C-15 Isoprenoid Analogues with Antibacterial Properties from the Whole Plant of Imperata cylindrica (L.) Raeusch (Gramineae)

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4717
Author(s):  
Romeo Nago ◽  
Paul Nayim ◽  
Armelle Mbaveng ◽  
James Mpetga ◽  
Gabin Bitchagno ◽  
...  
Keyword(s):  
Silica Gel ◽  
Methanol Extract ◽  
Antibacterial Properties ◽  
Imperata Cylindrica ◽  
Drug Resistant ◽  
Bacterial Strains ◽  
Prenylated Flavonoids ◽  
Whole Plant ◽  
Crude Methanol Extract ◽  
Potential Origin

The local botanical Imperata cylindrica in Cameroon was investigated for its antibacterial potency. The methanol extract afforded a total of seven compounds, including five hitherto unreported compounds comprising three flavonoids (1–3) and two C-15 isoprenoid analogues (4 and 5) together with known derivatives (6 and 7). The novelty of the flavonoids was related to the presence of both methyl and prenyl groups. The potential origin of the methyl in the flavonoids is discussed, as well as the chemophenetic significance of our findings. Isolation was performed over repeated silica gel and Sephadex LH-20 column chromatography and the structures were elucidated by (NMR and MS). The crude methanol extract and isolated compounds showed considerable antibacterial potency against a panel of multi-drug resistant (MDR) bacterial strains. The best MIC values were obtained with compound (2) against S. aureus ATCC 25923 (32 µg/mL) and MRSA1 (16 µg/mL).

scholarly journals Evaluation of Bioactivities of Gouania tiliaefolia Lam., an Indigenous Traditional Medicinal Plant of Bangladesh

2019 ◽  
Vol 22 (2) ◽  
pp. 200-207
Author(s):  
Tufael Ahmed ◽  
Rifat Khan ◽  
Nafisa Tabassum ◽  
Fahima Aktar ◽  
Mohammad Kaisarul Islam
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Analgesic Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Oral Glucose ◽  
Tail Flick ◽  
Cytotoxic Activities ◽  
Crude Methanol Extract

The crude methanol extract of Gouania tiliaefolia Lam. was partitioned by the modified Kupchan method and the fractions were evaluated for total phenolic content, antioxidant, cytotoxic, thrombolytic, hypotonic and heat-induced membrane stabilizing activities. The petroleum ether soluble fraction (PESF) and methanol extract (ME) showed the highest phenolic content of 78.30 ±1.60 mg and 70.37 ± 0.84 mg, respectively, which were expressed in gallic acid equivalent (GAE). Similar trends were observed in case of anti-oxidant and cytotoxic activities, where the PESF possessed the highest free radical scavenging activity and brine shrimp lethality (IC50 = 2.88 ± 0.02 μg/ml, LC50 = 2.59 ± 0.14 μg/ml), followed by ME (IC50 = 4.79 ± 0.17 μg/ml, LC50 = 3.38 ± 0.08 μg/ml) and CSF (IC50 = 37.51 ± 0.96 μg/ml, LC50 = 73.55 ± 0.26 μg/ml). In case of assays for thrombolytic and membrane stabilizing activities, all extractives showed insignificant results compared to the respective standards. The crude methanol extract of G. tiliaefolia was used to examine the in-vivo analgesic (central and peripheral), antidiarrheal and antidiabetic activities in Swiss albino mice. In case of castor oil induced diarrhea, the ME gave better reduction of diarrhea by 71.43% (at 400 mg/kg-body weight) compared to loperamide (64.29%). Antidiabetic activity was evaluated by oral glucose tolerance test and the ME showed 71.42% and 75.39% reduction of blood glucose at doses 200 and 400 mg/kg-body weight, respectively when compared with the standard glibenclamide that reduced blood glucose by 66.17%. The central- and peripheral-analgesic activity was evaluated by the tail-flick test and acetic acid induced writhing test, respectively. In both the cases, ME demonstrated dose-dependent analgesic activity compared to the standards. Bangladesh Pharmaceutical Journal 22(2): 200-207, 2019

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