scholarly journals Antibacterial and antifungal activity of Moringa oleifera stem bark

2013 ◽  
pp. 109-117 ◽  
Author(s):  
Md Shafiqur Rahman ◽  
Laila Zerin ◽  
MN Anwar
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Moringa Oleifera ◽  
Ethyl Acetate Extract ◽  
Pathogenic Fungi ◽  
Stem Bark ◽  
Shigella Dysenteriae ◽  
Antibacterial And Antifungal Activity ◽  
Antibacterial And Antifungal

Petroleum ether, chloroform, ethyl acetate and carbon tetrachloride extracts of Moringa oleifera stem bark were studied for their antimicrobial activities against eleven human pathogenic bacteria (Shigella dysenteriae, S. sonnei, Salmonella typhi, S. paratyphi, Bacillus subtilis, B. megaterium, B. cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Vibrio cholerae) and four human pathogenic fungi (Aspergillus niger, A. ochraceus, A. ustus and Candida albicans) using disc diffusion and poisoned food method, respectively. Chloroform and ethyl acetate extracts exhibited moderate to good antibacterial and antifungal activity against all the pathogens tested. The ethyl acetate extract exhibited the largest zone of inhibition (21 mm in diameter with 2000 mg/disc extract) against S. sonnei. The highest inhibition of fungal radial mycelial growth (52. 00% with 100 mg extract/ml medium) was recorded against C. albicans with ethyl acetate extract. The ethyl acetate extract exhibited the lowest MIC (750 mg/ml) against B. megaterium, S. dysenteriae, V. cholerae and E. coli. For fungi, the lowest MIC was 500 mg/ml against C. albicans with crude extract of ethyl acetate. DOI: http://dx.doi.org/10.3329/cujbs.v3i1.13411 The Chittagong Univ. J. B. Sci.,Vol. 3(1&2):109-117, 2008

scholarly journals Antibacterial and antifungal activity of crude extracts of Plumeria rubra L.

2013 ◽  
pp. 87-94
Author(s):  
Laila Jarin ◽  
Md Shafiqur Rahman ◽  
MN Anwar
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Shigella Dysenteriae ◽  
Antibacterial And Antifungal Activity ◽  
E Coli ◽  
Antibacterial And Antifungal ◽  
Ethyl Acetate Extracts ◽  
Plumeria Rubra

Petroleum ether, carbon tetrachloride, chloroform and ethyl acetate extracts of Plumeria rubra leaves were studied for their antimicrobial activities against eleven human pathogenic bacteria, viz., Shigella dysenteriae, S. sonnei, Salmonella typhi, S. paratyphi, Bacillus subtilis, B. megaterium, B. cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Vibrio cholerae and four human pathogenic fungi, viz., Aspergillus niger, A. ochraceus, A. ustus and Candida albicans using disc diffusion and poisoned food method, respectively. Chloroform and ethyl acetate extract exhibited moderate to good antibacterial and antifungal activity against all the pathogens tested. The ethyl acetate extracts exhibited the largest zone of inhibition (25 mm in diameter with 2000 mg/disc extract) against E. coli. The highest inhibition of fungal radial mycelial growth (62.00% with 100 mg extract/ml medium) was recorded against A. ustus with ethyl acetate extract. The MICs were determined by broth macrodilution technique. The ethyl acetate extract exhibited the lowest MIC (750 mg/ml) against E. coli. However, for fungi the lowest MIC was 500 mg/ml against A. ustus with the same extract. DOI: http://dx.doi.org/10.3329/cujbs.v3i1.13409 The Chittagong Univ. J. B. Sci.,Vol. 3(1&2):87-94, 2008

scholarly journals In vitro Antibacterial and Antifungal Activity of Borreria articularis

1970 ◽  
Vol 25 (2) ◽  
pp. 95-98 ◽  
Author(s):  
Razia Sultana ◽  
M Shafiqur Rahman ◽  
M Nazrul Islam Bhuiyan ◽  
Jaripa Begum ◽  
M Nurul Anwar
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Fungal Infections ◽  
Petroleum Ether Extract ◽  
Antibacterial And Antifungal Activity ◽  
Pure Compound ◽  
Alcohol Extract ◽  
Antibacterial And Antifungal

Petroleum ether extract, chloroform extract, ethyl acetate extract, ethyl alcohol extract and a pure compound 6-methyl-5-cyclodecen-1-ol obtained from aerial parts of Borreria articularis were studied for their antimicrobial activities against eleven human pathogenic bacteria and four human pathogenic fungi using disc diffusion and poisoned food method respectively. Ethyl acetate extract, ethanol extracts and the pure compound 6-methyl-5-cyclodecen-1-ol exhibited good antibacterial and antifungal activity against all the pathogens tested herein. The ethanol extract and 6-methyl-5-cyclodecen-1-ol exhibited the largest zone of inhibition (20 mm) at a concentration of 2,000 ìg/disc against Escheichia coli and Vibrio cholerae respectively. In case of fungi, the crude extract of ethanol and the pure compound exhibited the highest inhibition 53.5 and 52.0% of fungal radial mycelial growth (with 100 ìg/ml medium) against Aspergillus ustus and A. ochraceus respectively. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) of the pure compound 6-methyl-5-cyclodecen-1-ol were determined by broth macrodilution method. The lowest MIC (500 μg/ml) and MBC (1,000 μg/ml) were determined against V. cholerae. However, for fungi, the lowest MIC (750 μg/ml) and MFC (1,500 μg/ml) were recorded against A. ochraceus. The results suggest that active antimicrobial agent(s) present in the extracts of B. articularis may have potential for the treatment of bacterial and fungal infections. Keywords: Antimicrobial activity; Borreria articularis; Crude extracts; 6-Methyl-5 cyclodecen-1-olDOI: http://dx.doi.org/10.3329/bjm.v25i2.4869 Bangladesh J Microbiol, Volume 25, Number 2, December 2008, pp 95-98

scholarly journals Rauwolfia tetraphylla mediated synthesis of herbal nano powders by ball milling and GC-MS analysis, evaluation of their antimicrobial activity

2021 ◽  
Vol 12 (2) ◽  
pp. 967-977
Author(s):  
Vasa Padmaja ◽  
Gorrepati Rosaiah ◽  
Kakumanu Babu
Keyword(s):  
Antifungal Activity ◽  
Ball Milling ◽  
Pathogenic Bacteria ◽  
Nanoparticle Synthesis ◽  
Pathogenic Fungi ◽  
Salmonella Typhi ◽  
Gas Chromatography Mass Spectrometry ◽  
Antibacterial And Antifungal Activity ◽  
Ms Analysis ◽  
Antibacterial And Antifungal

Synthesis, characterization and evaluation of biological applications of nanoparticles is of immense importance in recent years in the field of Nanotechnology. Due to increasing demand of nanoparticle synthesis from medicinal plants now a days, in the present study, it is aimed to produce bio compatible, non-toxic, homogenous nanopowders by Ball milling, one of the top-down approaches from root, stem and leaf of Rauwolfia tetraphylla, an important medicinal plant of Apocynaceae. Rauwolfia tetraphylla L., an endangered species, is rich in various phytochemicals and often used as a substitute of Rauwolfia serpentina and therefore aimed to produce nanopowders from different parts of this plant. These biosynthesized nanopowders were characterized by UV-Visible Spectroscopy, Scanning Electron Microscopy, X-Ray Diffraction and Fourier Transform Infrared Spectroscopy. Further bioactive components of root, stem and leaf nanopowders were analysed by Gas Chromatography-Mass Spectrometry(GC-MS), and different compounds were identified by comparison of GC-MS spectrum with library searches. Methanolic root, stem and leaf nanopowders were evaluated for antibacterial and antifungal activity. Antibacterial activity was assessed against human pathogenic bacteria Klebsiella pneumoniae, Salmonella typhi, Vibrio cholerae, Corynebacterium diphtheriae, Vibrio parahemolyticus. Human pathogenic fungi Aspergillus fumigatus, Candida albicans, Mucor hiemalis were selected for evaluating antifungal activity. The present study confirms the good antibacterial and antifungal activity of Rauwolfia tetraphylla synthesized nanopowders. As GC-MS analysis revealed different phytoconstituents, other pharmacological activities need to be evaluated.

scholarly journals Phytochemical Analysis, Antibacterial and Antifungal Activities of Different Crude Extracts of Marine Red Alga Gracilaria corticata From The Gulf of Mannar South Coast, Navaladi, South India

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Manivachagam Chandrasekaran ◽  
Gnanaprakasam Adaikala Raj ◽  
Sakthivel Jegan ◽  
Venugopalan G. Venkatesalu
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Ethyl Acetate Extract ◽  
The Other ◽  
Disc Diffusion ◽  
Antibacterial And Antifungal Activity ◽  
Fungal Strains ◽  
Gracilaria Corticata ◽  
Antibacterial And Antifungal

To investigate the antibacterial and antifungal activity of hexane, chloroform, ethyl acetate, acetone and methanol extracts of Gracilaria corticata J. Ag against bacterial and fungal strains viz., Bacillus subtilis, Streptococcus pyogenes, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhimurium, Vibrio cholerae, Shigella flexneri, Proteus mirabilis and Proteus vulgaris. Fungal strains Candida albicans, Candida krusei, Candida guilliermondi, Candida parapsilosis, Candida tropicalis, Candida glabrata, four dermatophytes viz., Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum gypseum and Epidermophyton flocossum. The extracts of G. corticata were extracted with different solvents viz., hexane, chloroform, ethyl acetate and methanol against bacterial and fungal strains by using disc diffusion method, MIC, MBC and MFC were determined. The ethyl acetate extract of G. corticata showed the highest antibacterial and antifungal activity against all the bacterial and fungal strains tested than the other extracts. The mean zones of inhibition produced by the extracts in agar disc diffusion assays were from 7.1 to 16.0 mm. The Minimum Inhibitory Concentrations (MIC) was between 125 and 500?g/ml, while the Minimum Bactericidal Concentrations and Minimum Fungicidal Concentrations (MFC) were between 250 and 500?g/ml. The highest mean zone of inhibition (16.0 mm) was observed in ethyl acetate extract of G. corticata against B. subtilis. The lowest MIC (125?g/ml), MBC and MFC (250?g/ml) values was observed in ethyl acetate extract of G. corticata against B. subtilis. The results suggest that the effective ethyl acetate crude extract of G. corticata showed the presence of phytochemical, terpenoids, tannins and phenolic compounds strongly than the other solvent extracts. These finding suggest that ethyl acetate crude extract of G. corticata have potential antimicrobial activity are under going further analysis to identify the active compounds currently progress.

scholarly journals Antibacterial and Antifungal Activity of Heartwood of Acacia catechu of Nepal

2012 ◽  
Vol 27 ◽  
pp. 94-99 ◽  
Author(s):  
Susan Joshi ◽  
Yagya Prasad Subedi ◽  
Shobha Kumari Paudel
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Salmonella Typhi ◽  
Klebsiella Pneumonia ◽  
Zone Of Inhibition ◽  
Diethyl Ether Extract ◽  
Pseudomonas Species ◽  
Acacia Catechu ◽  
Antibacterial And Antifungal

Antibacterial activity of different extracts of heartwood of Acacia catechu were tested against 11 species of bacteria and antifungal activity were tested against four species of fungi. Among bacteria species highest zone of inhibition (ZOI) was measured against Pseudomonas species by diethyl ether extract, likewise among fungal organism highest zone of inhibition (ZOI) was measured against Fusarium oxysporim by ethyl acetate extract. No extracts showed activity against Salmonella typhi, Salmonella paratyphi, Escherichia coli and Proteus mirabilis. The minimum bactericidal concentration (MBC) of ethyl acetate extract was evaluated against Bacillus subtilis, Klebsiella pneumonia, Staphylococus aureus and Shigella species.DOI: http://dx.doi.org/10.3126/jncs.v27i1.6667 J. Nepal Chem. Soc., Vol. 27, 2011 94-99 

scholarly journals Evaluation of Antifungal, Hemolytic and Cytotoxic Potential of Ethyl Acetate Extract of a New Marine Streptomyces sp. AIAH-10

2016 ◽  
Vol 19 (1) ◽  
pp. 37-43
Author(s):  
Md Anwarul Haque ◽  
Ashish Kumar Sarker ◽  
Md Ajijur Rahman ◽  
Md Aktar Uzzaman Chouduri ◽  
Md Anwar Ul Islam
Keyword(s):  
Antifungal Activity ◽  
Mortality Rate ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Bioactive Molecules ◽  
Diffusion Method ◽  
Small Scale ◽  
Dilution Method ◽  
Streptomyces Sp

To face newly generated diseases, search for new, safe and effective bioactive molecules is highly warranted. The marine microbial flora may be a potential source of such molecules. The present study was designed to isolate marine microorganisms (AIAH-1 to AIAH-29) from the soil of mangrove forest Sundarbans, Bangladesh by serial dilution method using isolation media. Among them, AIAH-10 was selected for further study due to its promising antibacterial activity (done by streak plate and plug technique method) against a series of pathogenic bacteria. On the basis of morphological, cultural and biochemical analysis, the strain AIAH-10 belongs to Streptomyces sp. Secondary metabolites of the strain was obtained by small scale fermentation process. Antifungal activity of the extracts was performed by disc diffusion method. The crude ethyl acetate extract (50µg/disc) showed significant antifungal activity against Aspergillus niger, Candida albicans and Saccharromyces cerevaceae (14, 12 and 10 mm zone of inhibition respectively). No hemolytic activity was found of the extracts towards the human erythrocytes. During cytotoxic study against brine shrimp nauplii (Artemia salina), a dose dependent mortality rate was observed. A 100% larva mortality rate was recorded in 40µg/ml and more where as LC50 was found to be 6.61µg/ml.Bangladesh Pharmaceutical Journal 19(1): 37-43, 2016

Antibacterial and antifungal activity of essential oils against some pathogenic bacteria and yeasts shed from poultry

2016 ◽  
Vol 31 (4) ◽  
pp. 302-309 ◽  
Author(s):  
Valentina Virginia Ebani ◽  
Simona Nardoni ◽  
Fabrizio Bertelloni ◽  
Silvia Giovanelli ◽  
Guido Rocchigiani ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antibacterial And Antifungal Activity ◽  
Antibacterial And Antifungal

scholarly journals Antibacterial and Antifungal Activity Analysis of Essential Oil of Pogostemon cablin (Blanco) Benth

2016 ◽  
Vol 30 (1-2) ◽  
pp. 7-10 ◽  
Author(s):  
Puspa Das ◽  
Shoma Dutta ◽  
Jaripa Begum ◽  
Md Nural Anwar
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Bacillus Cereus ◽  
Pathogenic Bacteria ◽  
Pathogenic Fungi ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Pogostemon Cablin ◽  
Antibacterial And Antifungal

The essential oil of Pogostemon cablin (Blanco) Benth, also known as Patchouli oil was subjected for its antimicrobial investigation against a panel of ten human pathogenic bacteria and six human pathogenic fungi by Agar well diffusion method and Macrobroth dilution technique using Ampicillin (20ìg/well) and Nystatin (20ìg/well) as control. Antibacterial activity revealed that, the essential oil was more active against Gram positive bacteria than Gram negative bacteria. The largest zone of inhibition was 35 mm (against Bacillus cereus) with 20 ìl of oil. Ditermination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) showed that, Bacillus cereus exhibited the lowest MIC (250 ìg/ml) and MBC (750 ìg/ml). The oil showed moderate antifungal activity against all tested organisms. Candida albicans showed greater zone of inhibition (16 mm) than Saccharomyces cerevisiae (14 mm) with 20 ìl and Candida albicans showed lowest MIC and MFC (both were 750 ìg/ml). The zone of inhibition was 25 mm for each filamentous fungal strain with 20 ìl, except for Rhizopus oligosporus (15 mm) and the lowest MIC (250 ìg/ml) and MFC (500 ìg/ml) were reported for Aspergillus fumigatus.Bangladesh J Microbiol, Volume 30, Number 1-2,June-Dec 2013, pp 7-10

scholarly journals ASSESSMENT OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF A SPECIES OF ASPERGILLUS: AN ENDOPHYTIC FUNGUS OF SCHIMA WALLICHII (DC.) KORTH. LEAVES

2017 ◽  
Vol 10 (9) ◽  
pp. 361
Author(s):  
Sunrit Basu Sarbadhikary ◽  
Narayan Chandra Mandal
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Mode Of Action ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Pathogenic Fungi ◽  
Structural Features ◽  
Fungal Endophyte ◽  
Schima Wallichii

  Objective: The main goals of this study were to check the antimicrobial and antioxidant potentials of an endophytic fungal strain isolated from the leaves of Schima wallichii (DC.) Korth.Methods: The antibacterial and antifungal activities of the isolated fungal endophyte Visva-Bharati endophyte fungal (VBEF2) were checked by disc diffusion and agar well diffusion methods, respectively, against six pathogenic bacteria and four pathogenic fungi. The minimum inhibitory concentration (MIC) values and the mode of action of VBEF2 against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli were determined following colony-forming units (CFU) counting method. Antioxidant activity of the isolate was studied following 2, 2-diphenyl-2- picrilhydrazyl (DPPH) reduction assay.Results: The cell-free supernatants (CFS) of VBEF2 exhibited excellent antibacterial activity against all the bacteria used. The ethyl acetate extract of the endophyte was found to have the MIC of 50 μg/ml and 150 μg/ml against S. aureus and E. coli, respectively. It showed bactericidal mode of action against both of them. The CFS of the strain VBEF2 also showed excellent activities against two animal and two plant pathogenic fungi by producing zones of inhibition in the range of 10-20 mm. In the DPPH scavenging antioxidant assay, the ethyl acetate extract of VBEF2 was found with a low IC50 value of 19.01 μg/ml. The strain VBEF2 was identified as a species of Aspergillus based on its colony morphology and structural features observed under a compound light microscope.Conclusion: The strain VBEF2 can be implemented in various fields of pharmaceutical industry as it showed multidimensional beneficial attributes such as excellent antimicrobial and antioxidant activity.

scholarly journals Genome-based analysis of the type II PKS biosynthesis pathway of xanthones in Streptomyces caelestis and their antifungal activity

RSC Advances ◽  
2019 ◽  
Vol 9 (64) ◽  
pp. 37376-37383
Author(s):  
Ling-Li Liu ◽  
Hong-Fei Liu ◽  
Hua-Hua Gao ◽  
Zheng-Zhong Yang ◽  
Xiao-Lan Feng ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Pathogenic Fungi ◽  
Plant Pathogenic Fungi ◽  
Type Ii ◽  
Biosynthesis Pathway ◽  
Antifungal Activities ◽  
Liquid Fermentation ◽  
Type Ii Pks

The ethyl acetate extract from the liquid fermentation of S. caelestis Aw99c exhibited high and broad antifungal activities against plant pathogenic fungi.

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