scholarly journals Stereospermum suaveolens (Roxb.) DC. Shows Potential in vivo and in vitro Bioactivities

2018 ◽  
Vol 17 (2) ◽  
pp. 257-263
Author(s):  
Md Moniruzzaman ◽  
Md Ruhul Kuddus ◽  
Mohammad Rashedul Haque ◽  
AM Sarwaruddin Chowdhury ◽  
Mohammad A Rashid
Keyword(s):  
Salicylic Acid ◽  
Carbon Tetrachloride ◽  
Methanolic Extract ◽  
Soluble Fraction ◽  
Acetyl Salicylic Acid ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Oral Glucose ◽  
Tail Flick ◽  
Hypotonic Solution

The methanol extract of Stereospermum suaveolens (Roxb.) DC was investigated for antidiabetic, antidiarrheal and analgesic activities in Swiss Albino mice. Antidiabetic activity was evaluated by oral glucose tolerance test where the crude extract of S. suaveolens (400 mg/kg b.w.) exhibited 56.10% reduction of blood glucose level as compared to 58.53% by standard glibenclamide (0.1 mg/kg b.w.). In the castor oil-induced diarrhea in mice, the plant extract, at the dose of 400 mg/kg b.w. demonstrated 42.11% reduction of diarrheal feces, while the standard loperamide revealed 57.89% reduction of diarrheal feces. The analgesic activity of S. suaveolens was assessed by both radiant heat tail-flick and acetic acid-induced writhing test. The methanolic extract and different Kupchan fractions of S. suaveolens were also subjected to screening for total phenolic content, DPPH free radical scavenging assay, membrane stabilizing, thrombolytic and antimicrobial activities. In the DPPH assay, the aqueous soluble fraction of methanolic extract revealed highest antioxidant properties with IC50 value of 18.99 μg/ml. The membrane stabilizing activity was assessed by hypotonic solution- and heat-induced methods and was compared with standard acetyl salicylic acid. In hypotonic solution-induced haemolysis, the hexane and carbon tetrachloride soluble fraction inhibited 54.42% and 52.67% haemolysis of RBCs, respectively. On the other hand, in heat-induced haemolysis, the chloroform soluble fraction inhibited the haemolysis of RBC by 57.10% as compared to 72.09% produced by acetyl salicylic acid. In antimicrobial assay by disc diffusion method, only the hexane and carbon tetrachloride soluble fractions demonstrated moderate antimicrobial activity (zone of inhibition = 7.0-15.0 mm) against the test organisms. Dhaka Univ. J. Pharm. Sci. 17(2): 257-263, 2018 (December)

scholarly journals In vitro Bioactivities of Three Reputed Medicinal Plants of Bangladesh

2015 ◽  
Vol 17 (2) ◽  
pp. 147-150 ◽  
Author(s):  
Abu Bakar Siddique ◽  
Ridwan Islam ◽  
Md Al Amin Sikder ◽  
Ridwan Bin Rashid ◽  
AKM Nawshad Hossian ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Free Radical ◽  
Soluble Fraction ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Acetyl Salicylic Acid ◽  
Total Phenolic ◽  
Hypotonic Solution

The study was designed to evaluate the preliminary biological activities of crude methanol extracts of the leaf of Justicia gendarussa, Sansevieria trifasciata and Hydnocarpus kurzii and their organic soluble partitionates. The polyphenol content was determined spectrophotometrically and expressed as gallic acid equivalents. The total phenolic content was found to vary for different test samples ranging from 1.45 ± 0.25 mg to 40.73 ± 0.22 mg of GAE/ gm of dried extract. The antioxidant potential was evaluated by DPPH free radical scavenging assay using butylated hydroxytolune (BHT) and ascorbic acid as standards. In the assay, the CSF of H. kurzii and the PESF of J. gendarussa revealed the highest free radical scavenging activity with IC50 values 3.25 ± 0.05 ?g/ml and 24.68 ± 0.26 ?g/ml, respectively. The brine shrimp lethality bioassay was utilized to evaluate the cytotoxicity. The chloroform soluble fraction (CSF) and methanol extract (ME) of J. gendarussa exhibited strong cytotoxicity with LC50 values of 0.002 ?g/ml and 0.06 ?g/ml, respectively. The membrane stabilizing activity was assessed by evaluating hemolysis of RBC in hypotonic solution and was compared with acetyl salicylic acid. On the other hand, the chloroform soluble fraction of J. gendarussa and aqueous soluble materials of S. trifasciata produced 75.60% and 75.0% inhibition of hemolysis of RBC, respectively as compared to 77.9% inhibited by acetyl salicylic acid (0.10 mg/ml). DOI: http://dx.doi.org/10.3329/bpj.v17i2.22331 Bangladesh Pharmaceutical Journal 17(2): 147-150, 2014

scholarly journals Biological Screenings of Two Araceous Plants Growing in Bangladesh

2012 ◽  
Vol 10 (2) ◽  
pp. 131-135 ◽  
Author(s):  
Tanjina Hoque ◽  
Md Al Amin Sikder ◽  
Mohammad A Kaisar ◽  
Abu Asad Chowdhury ◽  
Mohammad A Rashid
Keyword(s):  
Carbon Tetrachloride ◽  
Crude Extract ◽  
Soluble Fraction ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Hypotonic Solution ◽  
Thrombolytic Activity ◽  
Vincristine Sulphate ◽  
Gallic Acid Equivalent

Two Bangladeshi medicinal plants from Araceae family, Alocasia indica and Steudnera virosa, have been investigated for their in vitro thrombolytic, membrane stabilizing antimicrobial activities and generalized    toxicity. The total phenolic content was also determined and expressed in gallic acid equivalent. The membrane stabilizing activity was assessed by heat and hypotonic solution induced methods. The carbon tetrachloride soluble  fraction (CTCSF) of A. indica and aqueous soluble fraction (AQSF) of S. virosa demonstrated strong membrane stabilizing activity. Different extractives of A. indica and S. virosa showed moderate thrombolytic activity. Among the two plants, the methanoic crude extract and its chloroform soluble partitionate of S. virosa revealed moderate inhibition of microbial growth. In brine shrimp lethality bioassay, the pet-ether and carbon tetrachloride soluble fractions of A. indica and the crude extract and its pet-ether soluble fraction of S. virosa were found to be as lethal as    Vincristine sulphate after 24 hours observation on shrimp nauplii.   DOI: http://dx.doi.org/10.3329/dujps.v10i2.11794   Dhaka Univ. J. Pharm. Sci. 10(2): 131-135, 2011(December)  

scholarly journals Evaluation of Thrombolytic, Membrane Stabilizing, Antidiarrhoeal and Analgesic Activities of Leaves of Triumfetta pilosa

2016 ◽  
Vol 19 (2) ◽  
pp. 226-232 ◽  
Author(s):  
Tanvir Ahmed ◽  
Amiyo Bala ◽  
Jalal Uddin ◽  
Zubair Khalid Labu
Keyword(s):  
Salicylic Acid ◽  
Reaction Times ◽  
Ethanolic Extract ◽  
Acetyl Salicylic Acid ◽  
Clot Lysis ◽  
Tail Flick ◽  
Hypotonic Solution ◽  
Immersion Method ◽  
Tail Immersion ◽  
Analgesic Activities

The aim of the research has been focused on the phytochemical investigation of the plants which have ethno-botanical and folkloric importance for drug discovery. The widespread availability and folkloric use of Triumfetta pilosa leaves led us to determine the pharmacological potential of the plant through in vitro and in vivo experiments. The crude ethanolic extract (EE) of T. pilosa leaves were partitioned successively using solvent of different polarities. Then these fractions were subjected to qualitative and quantitative phytochemical screening by standard procedures. The extract of T. pilosa and its fractions were evaluated for their possible thrombolytic, membrane stabilizing, antidiarrhoeal and analgesic activities by using standard drugs streptokinase, acetyl salicylic acid, loperamide and diclofenac-Na, respectively. In the study for thrombolytic activity, among all partitionates, the ethyl acetate soluble fraction (ESF) showed the highest percent of clot lysis (58.67%) as compared to standard streptokinase (69.23%) and water (3.77%). Also, in case of membrane stabilizing activity, ESF significantly inhibited the haemolysis of human erythrocyte membrane both induced by hypotonic solution (65.33 ± 0.50%) and heat (56.22 ± 0.69%), as compared to standard acetyl salicylic acid (ASA) (71.12 ± 26%) and (75.92 ± 0.29%), respectively. In the antidiarrheal assay, the crude ethanolic extract inhibited the mean number of defecation by 45.71% and 63.18% at 200 and 400-mg/kg body weight, respectively. During assay for central and peripheral analgesic activity at dose of 400-mg/kg, the extract showed reaction times of 5.11 (p < 0.001) and 1.96 (p < 0.001) min in the tail-flick and tail-immersion models, while the normal and reference groups exhibited reaction times of 11.66, 1.46; (p < 0.001) and 4.91, 1.16; (p < 0.001) in the tail flick and tail immersion method, respectively. At 400 mg/kg, the extract showed 49.22% inhibition of acetic acid induced writhing in mice model.Bangladesh Pharmaceutical Journal 19(2): 226-232, 2016

scholarly journals Phytochemical and biological investigation on Artocarpus chaplasha Roxb

IIUC Studies ◽  
2018 ◽  
Vol 14 (1) ◽  
pp. 45-50
Author(s):  
Sanjida Sharmin ◽  
Fakir Shahidullah Tareq ◽  
Choudhury Mahmood Hasan ◽  
Monira Ahsan
Keyword(s):  
Carbon Tetrachloride ◽  
Petroleum Ether ◽  
Methanolic Extract ◽  
Soluble Fraction ◽  
Biological Activities ◽  
Stem Bark ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Biological Investigation ◽  
Pure Compounds

The methanolic extract of the stem bark of Artocarpus chaplasha Roxb. (Family: Moraceae) was investigated for the isolation of pure compounds and determination of few biological activities. A mixture of two compounds cycloartenyl acetate and lupeol acetate were isolated from it. The crude methanol extract as well as its petroleum ether, chloroform, carbon tetrachloride and aqueous soluble fractions were studied for antioxidant, cytotoxic and antimicrobial activities. Among the different fractions, the aqueous soluble partitionate was found to have most potent antioxidant activity as compared to tertbutyl-1- hydroxytoluene. Antimicrobial screening of the different fractions was performed using the disc diffusion method. In these tests, the chloroform soluble fraction and carbon tetrachloride soluble fraction of methanolic extract exhibited moderate antimicrobial activity (range of zone of inhibition 8-12 mm). In brine shrimp lethality test, the petroleum ether soluble extract exhibited the highest level of cytotoxicity.IIUC Studies Vol.14(1) June 2017: 45-52

scholarly journals Wild Cinchona (Neolamarckia cadamba) – Bioactivities of a Medicinal Plant of Bangladesh

2016 ◽  
Vol 19 (1) ◽  
pp. 32-36
Author(s):  
Faiza Tahia ◽  
Mohidur Rahman ◽  
Marzia Bilkiss ◽  
Md Al Amin Sikder ◽  
Mohammad A Rashid
Keyword(s):  
Free Radical ◽  
Carbon Tetrachloride ◽  
Methanolic Extract ◽  
Soluble Fraction ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Vincristine Sulphate

The methanol extract of bark of Neolamarckia cadamba (Roxb.) and its organic and aqueous soluble partitionates were subjected to assays for antioxidant, cytotoxic, thrombolytic, membrane stabilizing, antimicrobial and analgesic activities. In the DPPH free radical scavenging assay, the pet ether and carbon tetra chloride soluble partitionate of the methanolic extract demonstrated the highest free radical scavenging activity with IC50 values of 60.46 ?g/ml and 78.58 ?g/ml, respectively. On the other hand, the carbon tetrachloride and pet ether soluble fractions displayed the potential lethality to brine shrimps, having LC50 of 7.24 and 7.89 ?g/ml, as compared to standard vincristine sulphate (LC50 value of 0.45 ?g/ml). During assay for thrombolytic property, the carbon tetrachloride and aqueous soluble materials revealed 66.36 % and 64.25 % clot lysis of human blood, respectively. In the membrane stabilizing assay, the carbon tetrachloride soluble fraction inhibited 86.79 % haemolysis of human RBCs in hypotonic solution-induced condition, while the aqueous soluble partitionate inhibited 92.39 % haemolysis of RBCs in the heat-induced condition. The crude methanolic extract of bark of N. cadamba showed significant central and peripheral analgesic activity at both 200 and 400 mg/kg body weight, whereas its chloroform soluble fraction mildly inhibited the growth of test microorganisms. Therefore, our studies suggest that wild cinchona should be subjected to extensive phytopharmacological investigation.Bangladesh Pharmaceutical Journal 19(1): 32-36, 2016

scholarly journals Bioactivities of Artocarpus chaplasha Roxb. and Bougainvillea spectabillis Willd.

2013 ◽  
Vol 16 (1) ◽  
pp. 63-68 ◽  
Author(s):  
Farjana Chowdhury ◽  
Senjuti Pal ◽  
Tasnuva Sharmin ◽  
Ridwan Bin Rashid ◽  
Md. Al Amin Sikder ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Crude Extract ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Radical Scavenging ◽  
Clot Lysis ◽  
Total Phenolic ◽  
Hypotonic Solution ◽  
Lysis Activity ◽  
Crude Methanol Extract

The methanol extracts of leaf of Artocarpus chaplasha Roxb. and Bougainvillea spectabillis Willd. as well as their petroleum ether, carbon tetrachloride, chloroform and aqueous soluble partitionates were subjected to screenings for antioxidant, cytotoxic, thrombolytic, membrane stabilizing and antimicrobial activities. The antioxidant potential was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Folin- Ciocalteau reagent using butylated hydroxytolune (BHT) and ascorbic acid as standards. The aqueous soluble fraction of the crude methanol extract of A. chaplasha and the methanolic crude extract of B. spectabillis demonstrated the highest free radical scavenging activity with the IC50 of 15.60±0.27 ?g/ml and 18.33±0.17 ?g/ml, which could be correlated to their total phenolic content of 61.26±0.23 and 50.00±0.19 mg of GAE/gm of extractive, respectively. Different extractives of A. chaplasha and B. spectabillis showed clot lysis activity ranging from 2.56±0.68% to 17.07±0.23% and 2.24±0.71% to 17.59±0.74% as compared to 66.77% and 3.791 % produced by the standard streptokinase and water, respectively. In hypotonic solution and heat induced conditions, the crude extract of A. chaplasha inhibited the haemolysis of human erythrocyte by 41.96±0.87% and 40.00±0.78%, while the carbon tetrachloride soluble fraction of methanol extract of B. spectabillis inhibited haemolysis by 65.68±0.36% and 35.62±0.82%, respectively. Here, acetyl salicylic acid (0.1 mg/ml) was used as reference showing 72.79% and 42.12% of haemolysis of RBCs in hypotonic and heat induced conditions, respectively. Among the extractives of both plants, the crude methanol extract of A. chaplasha and the chloroform soluble fraction of B. spectabillis extract revealed mild to moderate antimicrobial activity with zone of inhibition ranging from with 7.0 to 13.0 mm. The general toxicity was determined by brine shrimp lethality bioassay where the pet-ether soluble fraction of A. chaplasha (LC50 0.781±0.36 ?g/ml) and the aqueous soluble partitionate of B. spectabillis (LC50 1.28±0.57 ?g/ml) suggested the presence of considerable bioactive principles. DOI: http://dx.doi.org/10.3329/bpj.v16i1.14493 Bangladesh Pharmaceutical Journal 16(1): 63-68, 2013

scholarly journals Preliminary Biological Investigations of Lophopetalum fimbriatum and Calophyllum inophyllum

2015 ◽  
Vol 16 (2) ◽  
pp. 205-209 ◽  
Author(s):  
Md Al Amin Sikder ◽  
Razib Saha ◽  
Md Rokibuzzaman ◽  
Tasnuva Sharmin ◽  
Ridwan Bin Rashid ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Soluble Fraction ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Zone Of Inhibition ◽  
Calophyllum Inophyllum ◽  
Methanol Extracts ◽  
Soluble Fractions

The crude methanol extracts of the leaf of Lophopetalum fimbriatum (non Wight) F. Vill. and Calophyllum inophyllum L. as well as their pet-ether, carbon tetrachloride, chloroform and aqueous soluble fractions were evaluated for antioxidant, cytotoxic, thrombolytic, membrane stabilizing and antimicrobial activities. The antioxidant potential was evaluated by DPPH and Folin-Ciocalteau reagent using butylated hydroxytolune (BHT) and ascorbic acid as standards, respectively. Among the extractives of L. fimbriatum and C. inophyllum, the chloroform soluble fraction and methanolic crude extract demonstrated the highest free radical scavenging activity (IC50 = 175.57 ± 0.02 ?g/ml and IC50 = 1.0 ± 0.22 ?g/ml) which could be correlated with their total phenol contents 82.15 ± 0.89 and 32.19 ± 0.81 mg of GAE /g of extractives, respectively. In the brine shrimp lethality bioassay, the carbon tetrachloride soluble fractions of L. fimbriatum (LC50 = 0.515 ± 0.03 ?g/ml) and C. inophyllum (LC50 = 0.77 ± 0.18 ?g/ml) revealed general toxicity. During assay for thrombolytic activity, the carbon tetrachloride soluble materials of L. fimbriatum and the chloroform soluble fraction of leaf of C. inophyllum revealed clot lysis by 8.89 ± 1.410% and 27.84 ± 0.94%, while the standard streptokinase and water, used as positive and negative controls, demonstrated 66.77% and 3.79% clot lysis, respectively. In hypotonic solution and heat induced conditions, the crude methanol extracts of L. fimbriatum and C. inophyllum inhibited haemolysis of human erythrocyte by 68.14 ± 2.05% & 40.00 ± 1.6% and 57.67 ± 0.26% and 28.12 ± 0.38%, respectively. Here, acetyl salicylic acid (0.1 mg/ml) was used as reference showing 72.79% and 42.12% haemolysis of RBCs in hypotonic solution and heat induced conditions, respectively. The antimicrobial activity was assessed by the disc diffusion method and the chloroform soluble fraction of L. fimbriatum demonstrated 16.0 mm zone of inhibition against Sarcina lutea. Different extractives of C. inophyllum inhibited microbial growth with zone of inhibition ranging from 10.0 mm to 22.0 mm. Among the different extractives of C. inophyllum, the pet-ether and carbon tetrarchloride soluble fractions demonstrated 22.0 mm zone of inhibition against Vibrio parahaemolyticus and Pseudomonas aeruginosa, respectively. DOI: http://dx.doi.org/10.3329/bpj.v16i2.22305 Bangladesh Pharmaceutical Journal 16(2): 205-209, 2013

scholarly journals Evaluation of Bioactivities of Gouania tiliaefolia Lam., an Indigenous Traditional Medicinal Plant of Bangladesh

2019 ◽  
Vol 22 (2) ◽  
pp. 200-207
Author(s):  
Tufael Ahmed ◽  
Rifat Khan ◽  
Nafisa Tabassum ◽  
Fahima Aktar ◽  
Mohammad Kaisarul Islam
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Analgesic Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Oral Glucose ◽  
Tail Flick ◽  
Cytotoxic Activities ◽  
Crude Methanol Extract

The crude methanol extract of Gouania tiliaefolia Lam. was partitioned by the modified Kupchan method and the fractions were evaluated for total phenolic content, antioxidant, cytotoxic, thrombolytic, hypotonic and heat-induced membrane stabilizing activities. The petroleum ether soluble fraction (PESF) and methanol extract (ME) showed the highest phenolic content of 78.30 ±1.60 mg and 70.37 ± 0.84 mg, respectively, which were expressed in gallic acid equivalent (GAE). Similar trends were observed in case of anti-oxidant and cytotoxic activities, where the PESF possessed the highest free radical scavenging activity and brine shrimp lethality (IC50 = 2.88 ± 0.02 μg/ml, LC50 = 2.59 ± 0.14 μg/ml), followed by ME (IC50 = 4.79 ± 0.17 μg/ml, LC50 = 3.38 ± 0.08 μg/ml) and CSF (IC50 = 37.51 ± 0.96 μg/ml, LC50 = 73.55 ± 0.26 μg/ml). In case of assays for thrombolytic and membrane stabilizing activities, all extractives showed insignificant results compared to the respective standards. The crude methanol extract of G. tiliaefolia was used to examine the in-vivo analgesic (central and peripheral), antidiarrheal and antidiabetic activities in Swiss albino mice. In case of castor oil induced diarrhea, the ME gave better reduction of diarrhea by 71.43% (at 400 mg/kg-body weight) compared to loperamide (64.29%). Antidiabetic activity was evaluated by oral glucose tolerance test and the ME showed 71.42% and 75.39% reduction of blood glucose at doses 200 and 400 mg/kg-body weight, respectively when compared with the standard glibenclamide that reduced blood glucose by 66.17%. The central- and peripheral-analgesic activity was evaluated by the tail-flick test and acetic acid induced writhing test, respectively. In both the cases, ME demonstrated dose-dependent analgesic activity compared to the standards. Bangladesh Pharmaceutical Journal 22(2): 200-207, 2019

scholarly journals Evaluation of In Vitro Antimicrobial and Anti-Inflammatory Potentials of Crude Methanolic Extracts of Excoecaria agallocha (Leaves)

2016 ◽  
Vol 5 ◽  
pp. 25-33
Author(s):  
Md. Abdur Rahman ◽  
Md. Saddam Hussain ◽  
Md. Shalahuddin Millat ◽  
Md. Mizanur Rahman Moghal
Keyword(s):  
Methanolic Extract ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Hypotonic Solution ◽  
Gram Negative ◽  
Excoecaria Agallocha ◽  
Methanolic Extracts ◽  
Wide Range ◽  
Zones Of Inhibition

Excoecaria agallocha plant (Leaves) was analyzed to explore In Vitro antimicrobial and membrane stabilizing activities as a crude methanolic extract. Antimicrobial activity was performed against a wide range of Gram positive(+Ve) and Gram negative(-Ve) bacteria by using disc diffusion method and various methanolic extracts of leaves of E.agallocha was tested for determining membrane stabilizing activity at hypotonic solution and heat induce condition and standard acetyl salicylic acid (0.10 mg/mL) was employed as standard. the zones of inhibition created was found to be 1.3 cm at a concentration of 100 µL/ disc in case of Gram negative(-Ve) bacteria Salmonella typhi. The results obtained were compared with that of a standard Ampicillin (10 µL) and imipenem (10µL), penicillin (10µl), cefoxitine(30µl). Crude methanolic extract of 10mg/ml concentration showed maximum value of 17.67±0.0102 and 18.92±0.086% respectively under hypotonic solution and heat induced condition. Thus the result suggest that, crude methanolic extracts of E.agallocha possessed slight to moderate antimicrobial and membrane stabilizing properties.

scholarly journals In vitro Antioxidant, Membrane Stabilizing and Thrombolytic Activities of Glycosmis arborea

2012 ◽  
Vol 15 (2) ◽  
pp. 141-143 ◽  
Author(s):  
Sayed Mamur Hossain ◽  
Farhana Islam ◽  
Tasnuva Sharmin ◽  
Hasib Sheikh ◽  
AM Rashedul Hasan ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Pharmaceutical Journal ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Rat Erythrocytes ◽  
Thrombolytic Activity ◽  
Negative Controls

The methanol extract of leaves and stems of Glycosmis arborea and their pet-ether, carbon tetrachloride, chloroform and aqueous soluble partitionates were subjected to assays for antioxidant activity by Folin-Ciocalteau reagent, membrane stabilizing and thrombolytic activities. The carbon tetrachloride soluble fraction of stems of G. arborea demonstrated the presence of significant amount of phenolic compounds (36.95 ± 0.54 mg of GAE/g of extract). The extractives inhibited heat as well as hypotonic solution-induced haemolysis of rat erythrocytes in vitro. The pet-ether soluble fraction of leaves and stems of G. arborea showed 20.46 and 38.24% and 22.50 and 48.25%. inhibition of hemolysis of RBC caused by heat and hypotonic solution, as compared to 30.55 and 72.91% inhibition of hemolysis of RBC caused by the standard Acetyl salicylic acid at 0.01 mg/ml concentration, respectively. Among the four fractions, the chloroform soluble materials of the stems of G. arborea revealed highest thrombolytic activity with clot lysis value of 36.50% while standard streptokinase and water used as positive and negative controls, showed 64.25±0.26 and 2.35±0.35 % lysis of clot respectively DOI: http://dx.doi.org/10.3329/bpj.v15i2.12579 Bangladesh Pharmaceutical Journal 15(2): 141-143, 2012

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