scholarly journals Phytochemical and Biological investigations of Phoenix paludosa Roxb.

1970 ◽  
Vol 8 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Farzana Alam ◽  
Mohammad S Rahman ◽  
Md Shahanur Alam ◽  
Md Khalid Hossain ◽  
Md Aslam Hossain ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Brine Shrimp Lethality ◽  
First Report ◽  
Soluble Fractions

Lupeol (1), epilupeol (2) and β-sitosterol (3) were isolated from the n-hexane and the carbontetrachloride soluble fraction of a methanol extract of the leaves of Phoenix paludosa Roxb. The n-hexane, carbontetrachloride and chloroform soluble materials from the concentrated methanol extract were subjected toantimicrobial screening and brine shrimp lethality bioassay. All of the partitionates showed insensitivity to microbialgrowth, while the n-hexane, chloroform and methanol soluble fractions showed significant cytotoxicity having LC502.17 μg/ml, 2.77 μg/ml and 2.46 μg/ml, respectively. This is the first report of isolation of the compounds 1-3 andbioactivities of P. paludosa.Key words: Phoenix paludosa; Aracaceae; Lupeol; Epi-Lupeol; β-sitosterol; Brine shrimp lethality bioassay;Antimicrobial.DOI: 10.3329/dujps.v8i1.5329Dhaka Univ. J. Pharm. Sci. 8(1): 7-10, 2009 (June)

scholarly journals Phytochemical and Biological Investigations of Phyllanthus reticulatus

1970 ◽  
Vol 5 (1) ◽  
pp. 21-23 ◽  
Author(s):  
Taslima Begum ◽  
Mohammad S Rahman ◽  
Mohammad A Rashid
Keyword(s):  
Carbon Tetrachloride ◽  
Inhibitory Activity ◽  
Full Text ◽  
Brine Shrimp ◽  
Microbial Growth ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Stem Bark ◽  
Brine Shrimp Lethality ◽  
Soluble Fractions

Scopoletin (1) was isolated from the chloroform soluble fraction of a methanol extract of the stem bark of Phyllanthus reticulates (Family: Euphorbiaceae). The petroleum ether, carbon tetrachloride and choloroform soluble fractions of this methanol extract were subjected to antimicrobial screening and brine shrimp lethality bioassay. All of the partitionates showed moderate to strong inhibitory activity to microbial growth while the chloroform soluble fraction showed strongest cytotoxicity having LC50 1.99 ?g/ml. Key words: Phyllanthus reticulates, Euphorbiaceae, Scopoletin, Brine shrimp lethality bioassay, Antimicrobial Dhaka Univ. J. Pharm. Sci. Vol.5(1-2) 2006 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

scholarly journals Steroids from the Leave of Persicaria perfoliata (L.) H. Gross

2019 ◽  
Vol 22 (2) ◽  
pp. 224-227
Author(s):  
Md Sagar Hossain ◽  
NM Mofiz Uddin Khan ◽  
Md Saiful Quddus ◽  
AM Sarwaruddin Chowdhury
Keyword(s):  
Antimicrobial Activity ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Pharmaceutical Journal ◽  
Antimicrobial Screening ◽  
Brine Shrimp Lethality ◽  
Persicaria Perfoliata ◽  
Test Organisms

β-sitosterol (1) and β-sitosterol-D-glucoside (2) were isolated from the dichloromethane soluble fraction of a methanol extract of the leave of Persicaria perfoliata. The crude fraction was subjected to antimicrobial screening and brine shrimp lethality bioassay, where moderate antimicrobial activity was observed against most of the test organisms was seen. The hexane soluble fraction also displayed significant cytotoxic activity with LC50 0.64 μg/ml in brine shrimp lethality bioassay. This is the first report of occurrence of these compounds from this plant. Bangladesh Pharmaceutical Journal 22(2): 224-227, 2019

scholarly journals Preliminary Cytotoxicity and Antimicrobial Investigation of Anthocaphalus chinensis

2011 ◽  
Vol 3 (3) ◽  
pp. 689-692 ◽  
Author(s):  
M. A. Hossain ◽  
M. Z. Sultan ◽  
A. M. S. Chowdhury ◽  
C. M. Hasan ◽  
M. A. Rashid
Keyword(s):  
Carbon Tetrachloride ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Stem Bark ◽  
Microbiological Investigation ◽  
Brine Shrimp Lethality ◽  
Inhibition Of Growth ◽  
Test Organisms ◽  
Soluble Fractions

The methanol extract of the stem bark of Anthocephalus chinensis as well as its n-hexane, carbon tetrachloride, chloroform and aqueous soluble fractions were subjected to brine shrimp lethality bioassay and microbiological investigation. In the brine shrimp lethality bioassay, the aqueous soluble partitionate of the methanolic extract revealed the highest cytotoxicity having LC50 of 1.19 mg/ml while in case of antimicrobial screening, the chloroform soluble materials demonstrated moderate inhibition of growth of test organisms. Keywords: Anthocephalus chinensis; Rubiaceae; Cytotoxicity; Antimicrobial. © 2011 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. doi: 10.3329/jsr.v3i3.7529               J. Sci. Res. 3 (3), 689-692 (2011)

scholarly journals Isolation of Quercetin-3-O-beta-d-glucopyranoside from the Leaves of Azadirachta Indica and Antimicrobial and Cytotoxic screening of the Crude Extracts

2012 ◽  
Vol 60 (1) ◽  
pp. 11-14 ◽  
Author(s):  
Monirul Islam ◽  
MD Al-Amin ◽  
M Mahboob Ali Siddiqi ◽  
Shakila Akter ◽  
Mohammad Majedul Haque ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Azadirachta Indica ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Soluble Fraction ◽  
Ethanol Extract ◽  
Antimicrobial Screening ◽  
Brine Shrimp Lethality ◽  
Test Organisms ◽  
Soluble Fractions

Quercetin-3-O-beta-D-glucopyranoside was isolated from the ethylacetate soluble fraction of the ethanol extract of the fresh leaves of Azadirachta indica (Family: Meliaceae). The crude extract of hexane, ethylacetate and butanol soluble fractions of this ethanol extract were subjected to antimicrobial screening and brine shrimp lethality bioassay. The ethylacetate crude extract exhibited moderate antimicrobial activity against most of the test organisms and also showed significant cytotoxicity having LC50 0.61 µg/ml.DOI: http://dx.doi.org/10.3329/dujs.v60i1.10328Dhaka Univ. J. Sci. 60(1): 11-14, 2012 (January)

scholarly journals The Antimicrobial activity and Brine Shrimp Lethality Bioassay of Leaf extracts of Stephania japonica (Akanadi)

2012 ◽  
Vol 28 (2) ◽  
pp. 52-56 ◽  
Author(s):  
Rahman M Minhajur ◽  
M Masud Alam ◽  
A F M Shahid Ud Daula ◽  
Mohammad Shahriar ◽  
Md Mizanur Rahman Moghal ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Methanolic Extract ◽  
Soluble Fraction ◽  
Positive Control ◽  
Gram Negative Bacteria ◽  
Brine Shrimp Lethality ◽  
Vincristine Sulphate ◽  
Soluble Fractions

The purpose of the present study is to examine the antibacterial and cytotoxic properties of methanol extract of leaves of Stephania japonica. The crude methanolic extract of S. japonica, n-hexane, chloroform and ethyl acetate soluble fractions of methanolic extract were screened for their antimicrobial activity against a wide range of both Gram-positive and Gram-negative bacteria by disc diffusion method. The crude extract showed moderate and n-Hexane, chloroform soluble fraction of crude extract showed mild antibacterial activity against both Gram-positive and Gram-negative bacteria. Ethyl acetate soluble fraction of the extract showed significant antimicrobial activity against Salmonella typhi, Escherichia coli and Bacillus cereus. The zones of inhibition produced by the crude methanolic extract, n-hexane, chloroform and ethyl acetate soluble fractions were found to be 12.80-16.55 mm, 12.60 mm, 5-14.30 mm and 10-20.25 mm, respectively, at a concentration of 30 g/disc. Chloroform, n-hexane and ethyl acetate soluble fractions of methanolic extract of S. japonica were screened for antitumor properties using brine shrimp lethality bioassay. A reputed cytotoxic agent vincristine sulphate was used as a positive control. From the results of the brine shrimp lethality bioassay, it can be well predicted that chloroform and ethyl acetate soluble fractions of methanolic extract of S. japonica possess cytotoxic principles (with LC50 66.488 mg/ml and LC50 45.662 mg/ml, respectively) comparison with positive control vincristine sulphate (with LC50 0.839 mg/ml). But n-hexane soluble fractions of methanolic extract of S. japonica exhibited no lethality effect on shrimp nauplii. DOI: http://dx.doi.org/10.3329/bjm.v28i2.11816 Bangladesh J Microbiol, Volume 28, Number 2, December 2011, pp 52-56

scholarly journals Neoandrographolide Isolated from Leaves of Adhatoda vasica Nees

2012 ◽  
Vol 60 (1) ◽  
pp. 1-3 ◽  
Author(s):  
MD Al-Amin ◽  
Md Monirul Islam ◽  
M Mahboob Ali Siddiqi ◽  
Shakila Akter ◽  
Shamim Ahmed ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Soluble Fraction ◽  
Ethanol Extract ◽  
Adhatoda Vasica ◽  
Antimicrobial Screening ◽  
Brine Shrimp Lethality ◽  
Test Organisms ◽  
Soluble Fractions

Neoandrographolide was isolated from the ethylacetate soluble fraction of the ethanol extract of the fresh leaves of Adhatoda vasica (Family: Acanthaceae). The crude extracts of hexane, ethylacetate and butanol soluble fractions of ethanol extract were subjected to antimicrobial screening and brine shrimp lethality bioassay. The ethylacetate crude extract exhibited moderate antimicrobial activity against most of the test organisms and also showed significant cytotoxicity having LC50 0.61 µg/ml.DOI: http://dx.doi.org/10.3329/dujs.v60i1.10326Dhaka Univ. J. Sci. 60(1): 1-3, 2012 (January)

scholarly journals Phytochemical and Biological Investigations of Ixora lutea Hutch.

1970 ◽  
Vol 8 (1) ◽  
pp. 17-21 ◽  
Author(s):  
Shapna Sultana ◽  
Mohammad S Rahman ◽  
Md Aslam Hossain ◽  
Md Khalid Hossain ◽  
Mohammad A Rashid
Keyword(s):  
Antioxidant Activity ◽  
Petroleum Ether ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Preliminary Screening ◽  
Brine Shrimp Lethality ◽  
Growth Inhibitory ◽  
Growth Inhibitory Activity ◽  
Ic50 Value

Two triterpenoids, lupeol (1) and stigmasterol were isolated from the petroleum ether soluble fractionof the methanolic extract of Ixora lutea Hutch. In our preliminary screening, the petroleum ether, carbontetrachloride, chloroform soluble fractions of methanol extract of the leaves and stem were subjected to antioxidant,antimicrobial and brine shrimp lethality bioassay. All of the fractions showed moderate to potent antioxidant activity,of which the chloroform soluble fraction demonstrated the strongest antioxidant activity with the IC50 value of 3.0μg/ml. In case of antimicrobial screening, all extractives showed mild growth inhibitory activity. However, in thebrine shrimp lethality bioassay, the petroleum ether soluble fraction was found to be most cytotoxic among thepartitionates having LC50 value 0.938 μg/ml.Key words: Ixora lutea; rubiaceae; lupeol; stigmasterol; antioxidant activity; brine shrimp lethality bioassay;antimicrobial.DOI: 10.3329/dujps.v8i1.5331Dhaka Univ. J. Pharm. Sci. 8(1): 17-21, 2009 (June)

scholarly journals Investigation of Antioxidant, In-vitro Cytotoxic, and In-vivo Antitumor Effects of Leaf Extracts of Annona Reticulata

2016 ◽  
Vol 29 (2) ◽  
pp. 70-74
Author(s):  
Shashwata Biswas ◽  
Mohammad Shahriar ◽  
Jahan Ara Khanam ◽  
Chowdhury Rafiqul Ahsan
Keyword(s):  
Petroleum Ether ◽  
Crude Extract ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Control Group ◽  
Percentage Increase ◽  
Initial Weight ◽  
Antitumor Effects ◽  
Brine Shrimp Lethality ◽  
Annona Reticulata

This study was carried out to evaluate the antioxidant, brine shrimp lethality, bioassay and antitumor effects of nhexane, petroleum ether, methanol and chloroform extracts of Annona reticulata leaves. All extracts demonstrated good cupric reducing activity with increasing concentrations keeping methanol extract at the top and n-hexane extract at the second position. The methanol extract was found to have the highest antioxidant capacity 28±26.88 followed by n-hexane 16.95±11.39 and chloroform 12.85±10.32 in the form of mg of ascorbic acid equivalent per gram of crude extract. The methanol extract also showed the highest values of 2.41±0.963 mg of gallic acid equivalent phenolic content and 6.54±4.08 mg of quercetin equivalent flavonoid content per g of crude extract. In the brine shrimp lethality bioassay, LC50 values of methanol, chloroform, petroleum ether, and n-hexane extracts were found to be 1.084?g/ml, 1.096?g/ml, 1.995?g/ml and 3.126 ?g/ml, respectively. These findings suggest that A. reticulata leaves can be a good source of antioxidants and possess significant cytotoxic effect. In a narrow spectrum investigation on antitumor activity of the extracts, we found that the methanol extract inhibited the tumor growing process (control group, chloroform group and petroleum ether group showed significant rise in body weight; i.e., 8% - 14% of initial weight compared to that of methanol group showing a rise of 0.45% - 0.94% of initial weight) and improved ILS (Percentage Increase in Life Span) with increasing doses (Doses of 50mg/kg, 100mg/kg and 200mg/kg showed ILS values of 13.70%, 19.20% and 21.92%, respectively) to some extent when compared with control group and groups receiving other extracts.Bangladesh J Microbiol, Volume 29, Number 2, Dec 2012, pp 70-74

scholarly journals Antioxidant, Antibacterial and Cytotoxic Activity of the Methanol Extract of Urtica Crenulata

2009 ◽  
Vol 2 (1) ◽  
pp. 169-177
Author(s):  
M. A. Rahman ◽  
M. S. Rana ◽  
M. M. Zaman ◽  
S. A. Uddin ◽  
R. Akter
Keyword(s):  
Ascorbic Acid ◽  
Free Radical ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Brine Shrimp Lethality ◽  
Brine Shrimp Lethality Assay ◽  
Stem Extract

The antioxidant, antibacterial and cytotoxic properties of methanol extract of Urtica crenulata (syn: Laportea crenulata Gaud) stem has been investigated in the present study. The antioxidant and cytotoxic activity of the Urtica crenulata methanol extract were assessed by DPPH free radical scavenging method and brine shrimp lethality bioassay method, respectively. The antibacterial activity of the plant extract (500 μg/disc) was also carried out by disc diffusion technique. Stem extract showed DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging effect compared with ascorbic acid. IC50 value of ascorbic acid and stem extract was found 14.72 μg/ml and 1468.9 μg/ml, respectively. In antibacterial experiment, Urtica crenulata stem extract showed 8, 14 and 10 mm of diameter of zone inhibition against Salmonella typhi, Shigella flexneri and Shigella sonnei, respectively and 9 and 8 mm of diameter of zone inhibition against Bacillus subtilis and Bacillus cereus but no activity was observed against Staphylococcus aureus. In brine shrimp lethality assay, the LC50 value of the extract was found 104.0 μg/ml, which indicates that the extract has high cytoxic effect. The present study demonstrates that methanol extract of Urtica crenulata stem has significant cytotoxic effect. The extract also showed some moderate antibacterial and minimum significant antioxidant effects.  Keywords: Urtica crenulata;  Antioxidant; Antibacterial; Cytotoxic; BHT. © 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v2i1.2872             J. Sci. Res. 2 (1), 169-177 (2010) 

scholarly journals In Vitro Anticancer MCF-7, Anti-Inflammatory, and Brine Shrimp Lethality Assay (BSLA) and GC-MS Analysis of Whole Plant Butanol Fraction of Rheum ribes (WBFRR)

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Jahangir Khan Achakzai ◽  
Muhammad Anwar Panezai ◽  
Muhammad Ayub Kakar ◽  
Abdul Manan Kakar ◽  
Shahabuddin Kakar ◽  
...  
Keyword(s):  
Cell Line ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Aqueous Fraction ◽  
Brine Shrimp Lethality ◽  
Butanol Fraction ◽  
Whole Plant ◽  
Brine Shrimp Lethality Assay ◽  
Anti Inflammatory ◽  
Mcf 7

In this study, GC-MS analysis has shown that whole plant butanol fraction of rheum ribes (WBFRR) comprises of 21 compounds which exhibited anticancer (MCF-7) activity having IC50 value of 36.01± 0.26. MTT assay (MCF-7), Oxidative Burst assay using chemiluminescence technique, and B-Hatching techniques were the methods used for anticancer MCF-7, anti-inflammatory, and Brine Shrimp Lethality Assay (BSLA). GC-MS was used for structural elucidation. Whole plant methanol extract of rheum ribes (WMERR), whole plant n-hexane fraction of rheum ribes (WHFRR), and whole plant aqueous fraction of rheum ribes (WAFRR) were inactive against anticancer (MCF-7) cell line. Whole plant methanol extract of rheum ribes (WMERR), whole plant aqueous fraction of rheum ribes (WAFRR) and whole plant butanol fraction of rheum ribes (WBFRR) showed anti-inflammatory activity on ROS having IC50 value of 23.2±1.9, 24.2±2.7 and 12.0±0.6. Whole plant butanol fraction of rheum ribes (WBFRR) showed Brine Shrimp Lethality with LD50 693.302 while whole plant methanol extract of rheum ribes (WMERR) and whole plant aqueous fraction of rheum ribes (WAFRR) showed high lethality at highest concentration. This study revealed that whole plant butanol fraction of rheum ribes (WBFRR) exhibited significant anticancer (MCF-7) activity. In the near future, the constituent of whole plant butanol fraction of rheum ribes (WBFRR) can be the alternative drug against MCF-7 cell line with least toxicity and side effects.

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