scholarly journals Larvicidal effects of some plant seed extracts on Anopheles annularis Vander Wulp and Culex quinquefasciatus Say (Diptera: Culicidae)

2020 ◽  
Vol 5 (2) ◽  
pp. 41-52
Author(s):  
M Nasiruddin ◽  
MA Azadi ◽  
MR Chowdhury ◽  
RAH Tonni
Keyword(s):  
Ethyl Alcohol ◽  
Culex Quinquefasciatus ◽  
Mosquito Species ◽  
Exposure Period ◽  
Distilled Water ◽  
Chi Square ◽  
Plant Seed ◽  
Methanol Extracts ◽  
Average Temperature ◽  
Seed Extracts

Larvicidal effects of distilled water, 50% ethyl alcohol, acetone and methanol extracts on seed of four plants viz. Sinapis alba (Linn.), Carica papaya (Linn.), Momordica charantia (Linn.) and Capsicum annuum (Linn.) were studied upon the mosquito larvae of Anopheles annularis Vander Wulp and Culex quinquefasciatus Say under normal laboratory conditions with average temperature and relative humidity of 31.1oC and 57.6%, respectively. Percentage mortality, probit mortality, chi-square and ANOVA values for the larvae, extracts and concentrations were calculated for 24 hours exposure period. Mortality was dose dependent. The values of LC50 of the seeds of S. alba on An. annularis at the application doses for the distilled water, 50% ethyl alcohol, acetone and methanol extracts were 3431.791, 1038.363, 1254.810 and 2269.975 ppm, respectively; for C. papaya were 804.008, 403.294, 597.165 and 573.241 ppm, respectively; for M. charantia were 10593.241, 5017.710, 5650.191 and 6075.204 ppm, respectively; and for C. annuum were 1274.968, 4637.201, 1604.852 and 1905.692 ppm, respectively. The values of LC50 of S. alba seeds on Cx. quinquefasciatus at the application doses for the distilled water, 50% ethyl alcohol, acetone and methanol extracts were 1415.311, 432.996, 569.701 and 770.250 ppm, respectively; for C. papaya were 705.599, 385.688, 341.003 and 481.067 ppm, respectively; for M. charantia were 7764.154, 4496.464, 5353.759 and 5825.031 ppm, respectively; and for C. annuum were 2173.631, 1311.538, 78.748 and 182.869 ppm, respectively. Of the four plant seed extracts, C. papaya seed extracts were the most toxic for both the mosquito species. J. Biodivers. Conserv. Bioresour. Manag. 2019, 5(2): 41-52

scholarly journals Effectiveness of Three Fruit Seed Extracts as Larvicide against Three Major Mosquito Vectors Aedes aegypti Linnaeus, Culex quinquefasciatus Say and Anopheles gambiae Giles (Diptera: Culicidae)

2020 ◽  
pp. 16-29
Author(s):  
Lame Younoussa ◽  
Kary Mallam Oumarou ◽  
Theodora Kopa Kowa ◽  
Serge Eteme Enama ◽  
Gabriel Agbor Agbor ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Anopheles Gambiae ◽  
Culex Quinquefasciatus ◽  
Tap Water ◽  
Mosquito Species ◽  
Mangifera Indica ◽  
Larval Mortality ◽  
Persea Americana ◽  
Plant Seed ◽  
Seed Extracts

The CH2Cl2-MeOH (30:70 v/v) extracts of the seeds of Mangifera indica (Mango), Persea americana (Avocado) and Dacryodes edulis (African plum) were evaluated for potential mosquito larvicidal activity against 3rd and 4th instar larvae of Aedes aegypti, Culex quinquefasciatus and Anopheles gambiae. Extracts were diluted with 1 mL of methanol and concentrations ranging from 1000 to 125 mg/L in 4 replicates each, were prepared in the volume of 100 mL in the plastic cups (250 mL). A volume of 1 mL of methanol added to 99 mL of tap water was prepared as negative control and Bi-one (1000 mg/L) constituted a positive control. In each test solution, 25 larvae of each mosquito species were separately transferred and larval mortality was recorded after 24 h post-treatment. As results, the three plant seed extracts applied at 1000 mg/L caused for at least 79% mortality of each mosquito species larvae assessed. The seed extract of P. americana (LC50 of 98.31, 129.24 and 136.26 mg/L, respectively against An. gambiae, Ae. aegypti and Cx. quiquefasciatus larvae) was the most potent followed by D. edulis (LC50 of 176.87 mg/L for An. gambiae, 198.68 mg/L for Ae. aegypti and 201.70 mg/L for Cx. quinquefasciatus) and M. indica (LC50 of 258.98 mg/L for An. gambiae, 297.35 mg/L for Ae. aegypti and 435.45 mg/L for Cx. quinquefasciatus).  Globally, all the seed extracts were more toxic against An. gambiae larvae compared to other mosquito species and need further exploration for the development of a new botanical larvicide to reduce mosquito densities.

scholarly journals Toxic response of Heteropneustes fossilis (Bloch) to some indigenous plant seed extracts

2013 ◽  
pp. 01-12
Author(s):  
Munira Nasiruddin ◽  
Monika Rahman ◽  
Rozina Akhter
Keyword(s):  
Ethyl Alcohol ◽  
Water Extract ◽  
Distilled Water ◽  
Heteropneustes Fossilis ◽  
Absolute Ethyl Alcohol ◽  
Plant Seed ◽  
Alcohol Extract ◽  
Toxic Response ◽  
Indigenous Plant ◽  
Seed Extracts

Toxic responses of distilled water, 50% ethyl and absolute ethyl alcohol extracts of six indigenous plants, Datura innoxia (Mill), Clerodendrum viscosum (Vent), Amoora rohituka (Roxb) Wt. et. Arnott, Acacia auriculaeformis A. Cunn. ex. Benth., Pongamia pinnata (L) Pierre and Acacia catechu Willd were studied on a predatory fish Heteropneustes fossilis (Bloch) in laboratory conditions. Amongst the extracts the toxicity range varied, absolute ethyl alcohol extract of A. rohituka seeds being the most toxic (LC50 = 8.67 ppm) whilst distilled water extract of D. innoxia seeds was the least toxic (LC50 = 1950.03 ppm). On the basis of LC50 and relative potency values the relative toxicity of the six plants was in the order- A. rohituka > C. viscosum > P. pinnata > A. auriculaeformis > A. catechu > D. innoxia seed extracts. These six indigenous plant seed extracts assayed might be helpful as controlling agent of undesirable fishes in fish culture ponds. DOI: http://dx.doi.org/10.3329/cujbs.v4i1.13382 The Chittagong Univ. J. B. Sci.,Vol. 4(1&2):1-12, 2009

scholarly journals Toxicological effect and behavioral response of a predatory Stinging Catfish, Heteropneustes fossilis exposed to three indigenous plant seed extracts

2021 ◽  
Vol 48 (2) ◽  
pp. 379-391
Author(s):  
Sadia Sultana ◽  
Munira Nasiruddin ◽  
MA Azadi ◽  
MR Chowdhury
Keyword(s):  
Ethyl Alcohol ◽  
Fish Species ◽  
Water Extract ◽  
Predatory Fish ◽  
Distilled Water ◽  
Heteropneustes Fossilis ◽  
Plant Seed ◽  
Toxicological Effect ◽  
Indigenous Plant ◽  
Seed Extracts

For the removal of undesired fish species from aquaculture ponds, an alternative to synthetic toxins is the use of botanical toxicants which are biodegradable and environmentally safer. Toxicological effects of distilled water, 50% ethyl alcohol, methanol and acetone extracts of three indigenous plant seeds, Jatropha carcus (Linn.), Hydnocarpus wightianus (Blume) and Aleurites moluccana (Linn. Willd) were studied on the predatory fish, Heteropneustes fossilis (Bloch) under normal laboratory conditions. Depending upon the type of plants, extracts and concentrations, percentage mortality varied. Behavioral activities increased with increasing concentrations. During exposure, fish exhibited discolouration, gulping for air, erratic swimming, loss of reflex, slow movement and ultimately became motionless before death . The extent of toxicity of extracts for J. carcus, H. wightianus and A. moluccana on H. fossilis could be ranked in the order: Acetone > methanol > 50% ethyl alcohol > distilled water. On the basis of LC50 values the most toxic was acetone extract of J. carcus seeds and least toxic was distilled water extract of A. moluccana seeds. It is suggested that these plant seed extracts would be helpful in aquaculture to remove unwanted fish species from culture ponds. Bangladesh J. Zool. 48(2): 379-391, 2020

scholarly journals Potential of the Ethanol Extract of Aerial Parts of Diplazium esculentum (Retz) SW. AS Larvicide against Anopheles gambiae Giles and Culex quinquefasciatus Say

2020 ◽  
pp. 40-47
Author(s):  
I. A. Umohata ◽  
P. M. E. Ubulom ◽  
E. J. Udofa ◽  
D. N. Bala ◽  
I. V. James
Keyword(s):  
Anopheles Gambiae ◽  
Culex Quinquefasciatus ◽  
Mosquito Species ◽  
Differential Susceptibility ◽  
Ethanol Extract ◽  
Exposure Period ◽  
Plant Metabolites ◽  
Aerial Parts ◽  
Set Up ◽  
Anopheles Gambiae Giles

Plant products have been tested as insecticides against mosquitoes as they are promising candidates to replace conventional insecticides. This study was carried out to evaluate the larvicidal potential of ethanol extract of the aerial parts of Diplazium esculentum against Anopheles gambiae and Culex quinquefasciatus. Ethanol extract of the aerial parts of D. esculentum was screened for its phytochemical constituents and used for larvicidal assay. A stock solution of the extract (5 g in 100 ml of water) was prepared. From the stock solution, 0.45, 0.60, 0.75, 0.90 and 1.05% w/v concentrations of the extract were obtained for the study. Each concentration of the extract had 3 replicates. The control was also replicated. Twenty (20) third instar larvae each of Anopheles gambiae and Culex quinquefasciatus were separately exposed to each extract concentration for a duration of 48 hours. Larval nutrient was added to each experimental set up. Observations were made after 24 and 48 hours exposure period. Phytochemical screening revealed the presence of some plant metabolites. Mortality of larvae exposed to the extract increased with increased concentration and exposure time. This study revealed a differential susceptibility of larvae of the two mosquito species to the extract as evident by the 24 h LC50 values obtained which were 0.355 and 2.468% w/v for An. gambiae and Cx. quinquefasciatus respectively. Exposure of An. gambiae larvae to the extract resulted in 100% mortality even with the least concentration of 0.45% w/v after 48 hours exposure period while the highest concentration of extract (1.05% w/v) resulted in 53.33% mortality of Cx. quinquefasciatus larvae, after an exposure period of 48 hours. Results obtained from this study suggest that the aerial parts of D. esculentum if further explored would be useful in the control of An. gambiae and Cx. quinquefasciatus.

scholarly journals Efficacy of Petiveria Alliacea (Linneaus) leaf and root bark powder in the control of filariasis vector Culex Quinquefasciatus

2021 ◽  
Vol 82 (1) ◽  
Author(s):  
Michael Olarewaju Akintan ◽  
Joseph Onaolapo Akinneye ◽  
Oluwatosin Betty Ilelakinwa
Keyword(s):  
Synergistic Effect ◽  
Culex Quinquefasciatus ◽  
Mosquito Control ◽  
Exposure Period ◽  
Botanical Insecticides ◽  
Adult Mosquito ◽  
Root Bark ◽  
Plant Powders ◽  
Petiveria Alliacea ◽  
Feeding Activities

Abstract Background Mosquitoes are vectors of parasitic diseases such as malaria, lymphatic filariasis, yellow fever, and dengue fever among others. They are well known as public enemies for their noise nuisance, biting annoyance, sleeplessness, allergic reactions, and diseases transmission during the biting and feeding activities. This then necessitate the search for insecticides of plant origin which are bio-degradable, non-toxic, and readily available for man use. Result This study, evaluated the fumigant efficacy of the powder of P. alliacea to control the adult stage of Culex mosquito. Powder of Petiveria alliacea were administered at different dose of (1 g, 2 g, 3 g, 4 g, and 5 g), respectively. Result obtained shows the fumigant effect of the powder were effective with percentage mortality of 18.33–60.00% for the leaf powder and 23.30–71.60% for the root powder within 2 h post-treatment period (P < 0.05). The synergistic effect of the leaf and root powder was also investigated. The lethal dosage (LD50) of the leaf, root, and synergistic effect of leaf and root bark powder required to kill 50% of the adult Culex quinquefasciatus was 3.76 g, 2.86 g, and 2.63 g, respectively. However, 25.06 g, 15.25 g, and 12.94 g of the leaf, root, and leaf and root powder were required to kill 90% (LD90) after a 2-h exposure period. Conclusion These finding suggested P. alliacea powder could be a good source of insecticide which may be used for the production of biopesticides. The present findings have important implications in the practical control of adult mosquito by using botanical insecticides. These plant powders are easy to prepare, inexpensive, and safe for use in mosquito control.

scholarly journals Behavioural response of mosquito vectors Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus to synthetic pyrethroid and organophosphorus-based slow-release insecticidal paint

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Sunil Dhiman ◽  
Kavita Yadav ◽  
B. N. Acharya ◽  
Raj Kumar Ahirwar ◽  
D. Sukumaran
Keyword(s):  
Aedes Aegypti ◽  
Culex Quinquefasciatus ◽  
Anopheles Stephensi ◽  
Slow Release ◽  
Disease Risk ◽  
Mosquito Species ◽  
Escape Time ◽  
Behavioural Avoidance ◽  
Vector Mosquito ◽  
Contact Exposure

Abstract Background The direct toxicological impact of insecticides on vector mosquitoes has been well emphasized; however, behavioural responses such as excito-repellency and physical avoidance as a result of insecticide exposure have not been much studied. We have demonstrated the excito-repellency and behavioural avoidance in certain vector mosquito species on exposure to a slow-release insecticidal paint (SRIP) formulation in addition to direct toxicity. Methods A SRIP formulation developed by the Defence Research and Development Establishment, Gwalior, contains chlorpyriphos, deltamethrin and pyriproxyfen as active insecticides. Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti mosquitoes were used to study the excito-repellency response of the formulation. The experiments were performed in a specially designed dual-choice exposure and escape chamber made of transparent polymethyl methacrylate. For the experiments, the SRIP formulation was applied undiluted at a rate of 8 m2 per kg on 15 cm2 metallic surfaces. Mosquitoes were introduced into the exposure chamber, and observations of the movement of mosquitoes into the escape chamber through the exit portal were taken at 1-min intervals for up to 30 min. Results The evaluated formulation displayed strong excito-repellency against all three tested vector mosquito species. Results showed that the ET50 (escape time 50%) for Ae. aegypti, An. stephensi and Cx. quinquefasciatus was 20.9 min, 14.5 min and 17.9 min for contact exposure (CE) respectively. Altogether in CE, the escape rates were stronger in An. stephensi mosquitoes at different time intervals compared to Ae. aegypti and Cx. quinquefasciatus mosquitoes. The probit analysis revealed that the determined ET did not deviate from linearity for both non-contact exposure (NCE) and placebo exposure (PE) (χ2 ≤ 7.9; p = 1.0) for Ae. aegypti mosquitoes and for NCE (χ2 = 8.3; p = 1.0) and PE (χ2 = 1.7; p = 1.0) treatments in Cx. quinquefasciatus. Mortality (24 h) was found to be statistically higher (F = 6.4; p = 0.02) in An. stephensi for CE but did not vary for NCE (p ≥ 0.3) and PE (p = 0.6) treatments among the tested mosquito species. Survival probability response suggested that all the three tested species displayed similar survival responses for similar exposures (χ2 ≤ 2.3; p ≥ 0.1). Conclusion The study demonstrates the toxicity and strong behavioural avoidance in known vector mosquito species on exposure to an insecticide-based paint formulation. The combination of insecticides in the present formulation will broaden the overall impact spectrum for protecting users from mosquito bites. The efficacy data generated in the study provide crucial information on the effectiveness of the tested formulation and could be useful in reducing the transmission intensity and disease risk in endemic countries.

Evaluation of the effect of cetrimide with edta on Microhardness and surface changes in root dentin – An invitro study

2019 ◽  
Vol 11 (1) ◽  
pp. 3-11
Author(s):  
Dr. Swarooparanil Patil ◽  
Dr. B.S. Keshava Prasad
Keyword(s):  
Surface Erosion ◽  
Distilled Water ◽  
Test Results ◽  
Chi Square ◽  
Chi Square Test ◽  
Root Dentin ◽  
Group 3 ◽  
Post Hoc ◽  
Hardness Values ◽  
Surface Changes

The aim of the study was to evaluate the effect of different concentrations of cetrimide with or without EDTA solution on the microhardness and surface changes in root dentin. Single rooted human mandibular premolar teeth were decoronated and sectioned longitudinally. The root segments were horizontally embedded in autopolymerizing resin. These specimens were randomly divided into 6 experimental groups according to the irrigating solution used. The irrigating solutions used were 5% EDTA, 5%EDTA + 0.25% Cetrimide, 5%EDTA + 0.50% Cetrimide, 0.25% Cetrimide, 0.50% Cetrimide and distilled water. Each group was further divided into two subgroups. First subgroup specimens were employed for microhardness testing using vicker’s indenter. Second subgroup specimens were used to evaluate the surface changes of root dentin under stereomicroscope. Comparison of the mean microhardness of test groups was done using ANOVA followed by post hoc Tukey’s test. Pre- and post- treatment hardness values were compared using student paired t test. The data obtained from surface erosion scoring was subjected to statistical analysis using Chi square test. Results of this study showed significant reduction in the microhardness of dentin for all the groups except for the distilled water (p<0.001). The greatest reduction in dentin microhardness was observed with Group-3 (5% EDTA + 0.50% CTR). Specimens in 5% EDTA group showed greater erosion than other groups. Within the limitations of this study it was concluded that all the tested irrigating solutions reduced the microhardness of root dentin except distilled water. Maximum reduction in microhardness was observed with addition of 0.50% cetrimide to EDTA. EDTA increased the surface roughness of root dentin irrespective of cetrimide association. Use of cetrimide at concentrations higher than 0.25% would be detrimental to the microhardness of dentin.

scholarly journals MICROBIOLOGICAL ANALYSIS OF IN-USE TOOTHBRUSHES AND THEIR DECONTAMINATION USING DISINFECTION: A HOSPITAL BASED PILOT STUDY

2021 ◽  
Vol 9 (09) ◽  
pp. 614-620
Author(s):  
Shriya Choubey ◽  
◽  
Khyati Jain ◽  
Somendra K. Dhariwal ◽  
Ruchita Dixit ◽  
...  
Keyword(s):  
Pilot Study ◽  
Ethyl Alcohol ◽  
Randomized Control Trial ◽  
Microbiological Analysis ◽  
Distilled Water ◽  
Highly Effective ◽  
Randomized Control ◽  
Instructions For Use

Introduction and Aim: Purpose of this study is to sensitize healthcare givers about the significance of toothbrush acting as fomites and this study aimed at highlighting importance of disinfecting in-use toothbrushes. Material and Method: This was a randomized control trial involving 30 patients. 15 were given distilled water and 15 were given 70% ethyl alcohol for disinfecting in-use toothbrushes, which were then microbiologically tested after 7 days of use. Results: Finding of this study showed 70% alcohol was highly effective in decontaminating used toothbrushes. Conclusion: In-use toothbrushes become highly contaminated after use and can act as fomites for transmitting infections. Thus, proper instructions for use of toothbrushes should be given to patients and public.

scholarly journals Staining Interactions of Bixa Orellana (Annatto) Seed Extracts with Tissues of Wistar Rats

2019 ◽  
Vol 2 (2) ◽  
Author(s):  
Fasogbon Samuel Ayobami
Keyword(s):  
Column Chromatography ◽  
Wistar Rats ◽  
Blood Smear ◽  
Wistar Rat ◽  
Ethanol Solution ◽  
Giemsa Stain ◽  
Staining Reaction ◽  
Distilled Water ◽  
Bixa Orellana ◽  
Seed Extracts

Introduction: Bixa orellana is an ancestral multi use plant popularly known as Achiote or lipstick tree in view of its reddish – orange dye on its seeds, Central and South American populations used these seeds to color their bodies and lips, B. orellana is the only species of Bixaceae family. Aim: The aim of this study was to stain the organs such as skin, liver, kidney, brain, intestines, and bone marrow of a wistar rats with solutions of Bixa orellana (Annatto) seed extracts. Methods: The study design was experimental research, the seeds of Bixa orellana were extracted in distilled water, absolute ethanol and acetone, using maceration methods and the fractions of the extracts were determined using column chromatography while preliminary, the solutions of the extracts were used to stain tissues listed above at varying pH to establish staining interactions of the crude extracts and fractions eluted from the column chromatography. Results: The most significant staining interaction were observed in ethanol solution of bixa extract, followed by acetone and distilled water and staining were also observed at column fraction(9-26), with background yellowish appearance in all the staining solutions which was contrast to controls (haematoxylin and eosin) stained sections and no staining reaction was observed in blood smear of wistar rat. The staining interactions of great quality was observed at pH 5.0 and 7.5, despite its staining ability it was neither superior to eosin nor a substitute to giemsa stain due to the yellowish background appearance and its complete inactivity on blood smear. Conclusion: Bixa orellana dye extracts can be grouped under compound dyes or neutral dyes due to its interaction at both pH 5.0 and 7.5 and cannot be used as substitute for Giemsa stain.

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