Bacteriological Profile of Wound Swab and Their Antimicrobial Susceptibility Pattern in Shaheed Suhrawardy Medical College, Dhaka

2019 ◽  
Vol 11 (1) ◽  
pp. 65-68
Author(s):  
Mst Naznin Tarana ◽  
Jannatul Fardows ◽  
Nasreen Farhana ◽  
Rehana Khatun ◽  
Shahida Akter
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Wound Infection ◽  
Antimicrobial Susceptibility ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Susceptibility Pattern ◽  
Medical College ◽  
Antimicrobial Susceptibility Pattern ◽  
Wound Swab

Background: Wound infections are global problem in the field of surgery associated with long hospital stay, higher treatment expenditure, morbidity and mortality. Objective: To isolate and identify the bacteria causing wound infection and to determine the antimicrobial susceptibility pattern. Methods: This retrospective study was conducted in the Department of Microbiology at Shaheed Suhrawady Medical College, Dhaka from January 2017 to December 2017 for a period of one (01) year. A total of 190 wound swabs were collected from the patients who were visited in outpatient department and were admitted at inpatient department with skin and soft tissue infection. Swabs from the wound were inoculated on appropriate media and cultured and the isolates were identified by standard procedures as needed. Antimicrobial susceptibility testing was done by disc diffusion method according to ‘The Clinical Laboratory Standard Institute’ guidelines. Results: In this study, out of 190 cases 115 (60.52%) were male and 75 (39.47%) were female and majority 85(44.73%) were in the age group of 16 to 30 years. A total number of 190 isolates were obtained, among which 124 (65.25%) were culture positive cases. Among the isolated organisms predominant bacteria was Staphylococcus aureus 68 (35.79%) followed by Escherichia coli 30 (15.79%), Pseudomonas 14 (7.37%), Klebsiella 6 (3.16%), Proteus 4 (2.10%) and Acinetobacter 2 (1.05%). Staphylococcus aureus was sensitive to linezolid (94.11%), vancomycin (88.23%) and amikacin (70.58%). Among the Gram negative isolates Escherichia coli was predominant and showed sensitivity to imipenem (80%), amikacin (70%), ceftazidime (60%), piperacillin+ tazobactum (56.66%), colistin (53.33%). Pseudomonas showed sensitivity to colistin (78.57%), imipenem (71.42%). Klebsiella showed sensitivity to imipenem (83.33%), amikacin (66.66%), piperacillin+ tazobactum (66.66%), and colistin(66.66%). Conclusion: Staphylococcus aureus was the most frequently isolated pathogen from wound swab and the antibiotic sensitivity pattern of various isolates will guide for appropriate selection of antibiotics against wound infection, so as to reduce the spread of resistant bacteria. J Shaheed Suhrawardy Med Coll, June 2019, Vol.11(1); 65-68

scholarly journals Current Microbial Isolates from Wound Swab and Their Susceptibility Pattern in a Private Medical College Hospital in Dhaka city

2015 ◽  
Vol 3 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Shahin Sultana ◽  
Nurunnahar Mawla ◽  
Shamima Kawser ◽  
Nayareen Akhtar ◽  
Md Khoybar Ali
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Wound Infection ◽  
Antimicrobial Susceptibility ◽  
Clinical Laboratory ◽  
Diffusion Method ◽  
College Hospital ◽  
Medical College ◽  
Wound Swab ◽  
Sensitivity Pattern

Background: Wound infection is one of the major health problems that are caused and aggravated by the invasion of pathogenic organisms where empiric treatment is routine. Objective: To isolate and identify the bacteria causing wound infection and to determine the antimicrobial susceptibility pattern.Materials and method: A total of 263 wound swab and pus samples were collected during the period of January to December 2012 from Delta Medical College and Hospital, Dhaka, Bangladesh. Swabs from the wound were inoculated on appropriate media and cultured and the isolates were identified by standard procedures as needed. Antimicrobial susceptibility testing was performed by disk diffusion method according to ‘The Clinical Laboratory Standard Institute’ guidelines. Results: In this study 220 bacterial isolates were recovered from 263 samples showing an isolation rate of 83.65%. The predominant bacteria isolated from infected wounds were Staphylococcus aureus 89 (40.45%) followed by Escherichia coli 62 (28.18%), Pseudomonas aeruginosa 34 (15.45%), Enterococci 18 (8.18%), Acinetobacter 5 (2.27%), Klebsiella 9 (4.09%) and Proteus 3 (3.36%). Staphylococcus aureus was sensitive to linezolid (94.38%), fusidic acid (91.01%), vancomycin (87.64%), amikacin (74.15%) and gentamicin (73.03%). Among the Gram negative isolates Escherichia coli was predominant and showed sensitivity to imipenem (93.54%) amikacin (83.87%) colistin (53.22%) and piperacillin and tazobactum (53.22%) and pseudomonas showed sensitivity to amikacin (73.52%), imipenem (70.58%) and colistin (70.58%). Conclusion: Staphylococcus aureus was the most frequently isolated pathogen from wound swab and the antibiotic sensitivity pattern of various isolates help to assist the clinician in appropriate selection of empirical antibiotics against wound infection. DOI: http://dx.doi.org/10.3329/dmcj.v3i1.22236 Delta Med Col J. Jan 2015; 3(1): 25-30

scholarly journals Antimicrobial Susceptibility Pattern and ESBL Prevalence of Bacteria Isolated from Street Vended Snacks

2021 ◽  
pp. 74-80
Author(s):  
Chidiebere Anab-Atulomah ◽  
Ngozi C. Oji ◽  
Vivian N. Kanu
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Antimicrobial Susceptibility ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Susceptibility Pattern ◽  
Laboratory Unit ◽  
Antimicrobial Susceptibility Pattern ◽  
Significant Difference ◽  
Esbl Producers

Aims: The objective of the study was to ascertain the antimicrobial susceptibility pattern and ESBL prevalence of bacteria isolated from snacks. Place and Duration of Study: Department of Microbiology (Laboratory Unit) Michael Okpara University of Agriculture Umudike. Methodology: The snacks were mashed aseptically, serially diluted and inoculated onto nutrient agar and MacConkey agar. Isolates were identified using standard microbiological procedures. Antimicrobial susceptibility of the isolates and ESBL detection was done using disk diffusion method. ESBL production was confirmed using Double Disc Synergy Test (DDST) method following CLSI recommendations. Results: Escherichia coli, Salmonella Typhi, Staphylococcus aureus and Klebsiella pneumoniae were the bacteria isolated with Escherichia coli as the most prevalent isolate with 42% occurrence in the samples screened. There was significant difference in the sensitivity of the bacteria isolates to the different antibiotics used at P=0.05. Salmonella Typhi isolates exhibited highest resistance to an antibiotic with 86% resistance to ciprofloxacin while Klebsiella pneumoniae isolates exhibited the lowest resistance to an antibiotic with 10% resistance to cefotaxime. Among the Gram-negative bacteria, 36% of suspected ESBL producing E. coli isolates were confirmed as ESBL producers indicating the highest occurrence. Conclusion: The study confirmed the presence of bacteria in street vended snacks which exhibited high resistance to antibiotics that could be attributed to the presence of ESBL producers among the isolates.

scholarly journals Antimicrobial Susceptibility Patterns of Escherichia Coli From Various Clinical Sources

2013 ◽  
Vol 3 (1) ◽  
pp. 14-17 ◽  
Author(s):  
R Gautam ◽  
ML Chapagain ◽  
A Acharya ◽  
N Rayamajhi ◽  
S Shrestha ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Tract Infection ◽  
Antimicrobial Susceptibility ◽  
E Coli ◽  
Medical College ◽  
Antimicrobial Susceptibility Pattern ◽  
Wound Swab ◽  
Highly Sensitive ◽  
High Degree ◽  
Susceptibility Patterns

Escherichia coli is the major organism causing the urinary tract infection, wound infection and respiratory tract infection. A total of 2376 samples of urine, wound swab and sputum were analyzed for identification of bacterial isolates and their antimicrobial susceptibility pattern. Escherichia coli (E. coli) was isolated from 357 [15%] samples. Majority of the E.coli, 307 [85.9%], were obtained from the urine samples, followed by wound swab [8.4%] and sputum [5.6%]. High degree of resistance was observed for nalidixic acid [92.8%] followed by ceftriaxone [65.7%] and cotrimoxazole [64.6%]. The isolates were highly sensitive to imipenem (100%) followed by nitrofurantoin [90.3%] and amikacin [82.1%]. Journal of Chitwan Medical College 2013; 3(1): 14-17 DOI: http://dx.doi.org/10.3126/jcmc.v3i1.8459

scholarly journals Escherichia coli O157:H7 in Retail Lettuce (Lactuca sativa) in Addis Ababa City: Magnitude of Contamination and Antimicrobial Susceptibility Pattern

2021 ◽  
Vol 12 ◽  
Author(s):  
Aklilu Feleke Haile ◽  
Silvia Alonso ◽  
Nega Berhe ◽  
Tizeta Bekele Atoma ◽  
Prosper N. Boyaka ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Addis Ababa ◽  
Foodborne Pathogen ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Susceptibility Pattern ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Antimicrobial Susceptibility Pattern

Escherichia coli O157:H7 is an important foodborne pathogen but largely under investigated in Africa. The objectives of this study were to estimate the prevalence and pattern of antimicrobial resistance of E. coli O157:H7 in lettuce in Addis Ababa, Ethiopia. A total of 390 retail lettuce samples were collected across the 10 subcities of Addis Ababa. E. coli O157:H7 was isolated and identified following ISO-16654:2001 standard. The isolates were further tested for antimicrobial susceptibility to 13 antimicrobials using the Kirby–Bauer disk diffusion method. Out of the 390 lettuce samples examined, two (0.51%) carried E. coli O157:H7. The antimicrobial susceptibility pattern of strains showed resistance to ampicillin (100%) and tetracycline (50.0%). One of the two isolates was multidrug resistant to two antimicrobials tested. The results of this study demonstrate the presence of drug-resistant E. coli O157:H7 in lettuce in markets in Addis Ababa. Despite the low prevalence, its presence in a product that is eaten raw highlights potential public health risk in the area associated with this pathogen.

scholarly journals Isolation and Antimicrobial Susceptibility Pattern of Urinary Escherichia Coli in Dhaka Medical College Hospital, Bangladesh

2017 ◽  
Vol 7 (1) ◽  
pp. 40-44
Author(s):  
Tashmin Afroz Binte Islam ◽  
SM Shamsuzzaman ◽  
Irin Rahman ◽  
Faria Ferdousi
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Medical College Hospital ◽  
Resistance Pattern ◽  
Susceptibility Pattern ◽  
Biochemical Tests ◽  
Test Reaction ◽  
College Hospital ◽  
Medical College ◽  
Antimicrobial Susceptibility Pattern

Background: Escherichia coli is the major bacterial pathogen being isolated and reported from urine, globally. For these uropathogens the therapeutic management becomes limited, so knowledge about the antimicrobial resistance pattern of these agents at a specific area may help the doctors to choose correct treatment regimen. The aim of the current study was to detect the common uropathogens from urine and the antimicrobial susceptibility pattern of Escherichia coli in Dhaka Medical College Hospital (DMCH).Methods: Samples were collected over a period of 12 months from July 2011 to June 2012 from the patients of DMCH irrespective of age and sex. Samples were cultured in blood agar and MacConkey agar media and organisms were identified by different biochemical tests such as oxidase test, reaction in MIU and simmon's citrate media and different sugar fermentation tests. Data were analyzed using Microsoft Excel (2007).Results: From total 300 urine samples, 177 (59%) Gram-positive and Gram-negative bacteria were isolated. Among them, Esch. coli was the most predominant 112 (63.28%) organism followed by Enterobacter spp. (11.87%). The prevalence of Esch. coli was significantly higher in females (63.39%) of younger age group than in males (36.61%). Esch. coli was highly sensitive to imipenem (100%) followed by ceftriaxone (62.50%), ceftazidime (55.36%) and azitromycin (35.72%) respectively. Low sensitivity patterns were found against doxycycline (13.39%), co-trimoxazole (16.07%), amoxiclav (21.43%) and ciprofloxacin (23.22%) respectively.Conclusion: The result of this study provides insight into the high proportion of multidrug resistant Escherichia coli and creates a need to report them routinely in laboratories.Anwer Khan Modern Medical College Journal Vol. 7, No. 1: Jan 2016, P 40-44

scholarly journals Antimicrobial susceptibility pattern of Enteropathogenic Escherichia coli (EPEC) in paediatric diarrhoeal patients

2015 ◽  
Vol 8 (1) ◽  
pp. 12-16
Author(s):  
Shimu Saha ◽  
Sanya Tahmina Jhora ◽  
Tanjila Rahman
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Tertiary Care ◽  
Diffusion Method ◽  
Enteropathogenic Escherichia Coli ◽  
Susceptibility Pattern ◽  
Antimicrobial Susceptibility Pattern ◽  
Number Of Children ◽  
Improper Use ◽  
Stool Samples

Enteropathogenic Escherichia coli (EPEC) mediated infantile diarrhoea among children is an important cause of morbidity and mortality in developing countries. The antimicrobial susceptibility pattern of EPEC strains isolated from children under 5 years of age was studied. Stool samples from 272 patients with diarrhoea were collected from two tertiary care hospitals. Out of 272 stool samples, 20 (7.35%) isolates were identified as EPEC on the basis of presence of bfpA gene detected by polymerase chain reaction and antibiotic susceptibility testing was performed on these EPEC strains by Kirby-Bauer disc diffusion method. The antimicrobial susceptibility test revealed that the EPEC isolates were highly resistant to ampicillin (100%), nalidixic acid (95%) and tetracycline (95%) and were sensitive to ceftazidime (95%), cefotaxime (90%), ceftriaxone (95%), imipenem (100%) and levofloxacin (85%). Isolation of EPEC is of great importance since they are responsible for acute diarrhoeal diseases in large number of children under the age of five years. The high antimicrobial resistance observed in our study indicates indiscriminate or improper use of antimicrobials, besides the risks of self-medication.Ibrahim Med. Coll. J. 2014; 8(1): 12-16

scholarly journals Bacteriology of urinary tract infection in pediatric patients at KIST Medical College Teaching Hospital

2014 ◽  
Vol 3 (1) ◽  
pp. 21-25 ◽  
Author(s):  
Birendra Raj Raghubanshi ◽  
Devendra Shrestha ◽  
Mahesh Chaudhary ◽  
Bal Man Singh Karki ◽  
Ajaya Kumar Dhakal
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antimicrobial Susceptibility ◽  
Demographic Data ◽  
College Teaching ◽  
Susceptibility Pattern ◽  
Medical College ◽  
Antimicrobial Susceptibility Pattern

Background: Urinary tract infection is common in children and is an important cause of morbidity. Urinary tract infection at young age can lead to renal injury and scarring, and ultimately lead to end stage renal disease in adulthood. Objectives: The purpose of study was to identify the different species of microorganisms, along with their antimicrobial susceptibility pattern, causing urinary tract infection in paediatric patients presenting with urinary tract infection at KIST Medical College, Imadol, Lalitpur, Nepal. Methods: This retrospective study examined microbiological and antimicrobial susceptibility pattern for urine samples collected at KIST Medical College, Imadol, Lalitpur from December 2010 to November 2013. A urine sample was included in our dataset if it demonstrated pure growth of a single organism and accompanying antimicrobial susceptibility and subject demographic data were available. Results: Escherichia coli was the most common organism isolated, followed by Klebsiella species, Staphylococcus species and then by Proteus species, Enterococcus species and Citrobacter species being equal in number. Microorganisms were most susceptible to amikacin and nitrofurantoin and most resistant to ampicillin and nalidixic acid. Conclusion: Though various microorganisms are responsible for urinary tract infection in children, Escherichia coli is the most common causative agent. Antimicrobial resistance has already emerged against many antibiotics, making empiric treatment of these infections challenging. DOI: http://dx.doi.org/10.3126/jkmc.v3i1.10919Journal of Kathmandu Medical CollegeVol. 3, No. 1, Issue 7, Jan.-Mar., 2014, Page: 21-25

scholarly journals fFrequency of Nasal Carriage of Staphylococcus aureus Among Healthcare Workers (HCWs) and Patients in Bandar Abbas, Southern Iran

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Motahare Amirizadeh ◽  
Ali Rahimi ◽  
Maryam Ansari ◽  
Ali AtashAbParvar ◽  
Hosein Hamadiyan ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Healthcare Workers ◽  
Methicillin Resistance ◽  
Nasal Carriage ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Susceptibility Pattern ◽  
Antimicrobial Susceptibility Pattern ◽  
Bandar Abbas

Background: Staphylococcus aureus is a problematic infectious agent in hospitals as well as in the community. Nasal carriage of healthcare workers (HCWs) and sometimes patients are an important source for transmitting this bacterium to vulnerable individuals. Objectives: The present study aimed to investigate the frequency of nasal carriage of S. aureus and the antimicrobial susceptibility pattern of this organism isolated from HCWs and patients at Shahid Mohammadi Hospital in Bandar Abbas, South of Iran. Methods: This cross-sectional study was conducted from November 2017 to December 2018. A total of 400 nasal swabs were taken from HCWs and patients to investigate the presence of S. aureus. An antimicrobial susceptibility pattern was carried out using the disc diffusion method according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Methicillin resistance was determined using cefoxitin disc diffusion and PCR for mecA gene. Agar dilution was performed to determine MIC of vancomycin and mupirocin. Results: Of 130 HCWs, 11 (8.5%) subjects were nasal carriers, of which 5 (45.5%) harbored methicillin-resistant Staphylococcus aureus (MRSA). Of 270 patients, 21 (7.8%) patients were nasal carriers, of whom 9 (42.9%) patients were MRSA carriers. Linezolid and vancomycin were the most effective agents, and 100% of isolates were susceptible to these agents. Furthermore, high-level mupirocin-resistant S. aureus (HLMuRSA) was observed in 6.3% of the isolates. Conclusions: Our findings demonstrate that the rate of nasal carriage among HCWs and patients was lower than global reports. However, the frequency of MRSA was comparable with previously reported ranges and was approximately high. Vancomycin and linezolid are the most effective antimicrobial agents. Appropriate decolonization is recommended for the control of transmission of MRSA to vulnerable individuals.

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