scholarly journals Microbiological investigation and determination of the antimicrobial potential of cow dung samples

2019 ◽  
Vol 8 (1) ◽  
pp. 34-37 ◽  
Author(s):  
Saurab Kishore Munshi ◽  
Juel Roy ◽  
Rashed Noor
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Cow Dung ◽  
Agricultural Field ◽  
Salmonella Spp ◽  
Antimicrobial Potential ◽  
Bacillus Spp ◽  
Bacteria And Fungi ◽  
Staphylococcus Spp

Cow dung is being used in agriculture as well for the household and religious purposes from the ancient time. Cow dung is known to possess antimicrobial activity and contains a wide variety of microorganisms with variable properties. Therefore, the present study was carried out to assess the microbial diversity including pathogenic ones of the cow dung samples as well as to determine the antimicrobial traits of the samples. In this regard, a total of 8 fresh cow dung samples were tested. All the samples contained a huge load of bacteria and fungi in an average of 108 and 107 cfu/g, respectively. An extended number of pathogenic bacteria were recovered. Among the pathogenic bacteria, Staphylococcus spp. and Bacillus spp. were predominantly found in every sample. The presence of Pseudomonas spp. Escherichia coli, Klebsiella spp. and fecal coliform were exhibited in most of the samples. Salmonella spp. and Vibrio spp. were found in 3 and 5 samples, respectively. The average load of the pathogens was 104 cfu/g. All the samples showed substantial degree of antimicrobial activity against all the pathogens tested. Samples 1, 2, 3 and 4 were effective in inhibiting the growth of all the tested pathogens. The findings of the present study revealed the need for safe application of cow dung in the agricultural field and of further investigation for the antimicrobial potential of the diversified microflora of cow dung offering agricultural, environmental and medical applications. Stamford Journal of Microbiology, Vol.8(1) 2018: 34-37

scholarly journals Influence of tannery wastes and the surrounding environment of tannery industries on microbial growth and proliferation

2017 ◽  
Vol 7 (1) ◽  
pp. 14-18
Author(s):  
Nayan Chandra Das ◽  
Farzana Hossaini ◽  
Saurab Kishore Munshi
Keyword(s):  
Pathogenic Bacteria ◽  
Multidrug Resistant ◽  
Salmonella Spp ◽  
Tannery Waste ◽  
Surrounding Environment ◽  
Bacillus Spp ◽  
Tannery Wastes ◽  
Bacteria And Fungi ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

The present study was carried out to assess the degree of microbiological proliferation in tannery wastes and the surrounding environment of the tannery industries. In this regard, a total of 8 tannery waste (n=4) and environmental (n=4) samples were tested. All the samples contained a huge load of bacteria and fungi in an average of 108 cfu/g or ml. An extended numbers of pathogenic bacteria were recovered. Among the pathogenic bacteria, Staphylococcus spp. was predominant. Most of the samples exhibited the presence of Pseudomonas spp. Salmonella spp. and fecal coliform were found each in one sample. Bacillus spp., Escherichia coli, Klebsiella spp. and Vibrio spp. were found in few samples. The average load of the pathogens was 104 cfu/g or ml. All the pathogenic isolates were found to be multidrug resistant. Higher resistance was found against penicillin and streptomycin. Tannery waste after lather treatment sample showed antibacterial activity against all the pathogens tested. Overall, presence of pathogenic microorganisms with multidrug resistance traits may pose serious public health threats. Stamford Journal of Microbiology, Vol.7(1) 2017: 14-18

scholarly journals Evaluation of sanitary quality of goat milk in dairy industries from the Cariri region, state of Paraíba

2021 ◽  
Vol 10 (12) ◽  
pp. e596101220735
Author(s):  
Iara Nunes de Siqueira ◽  
Aline Antas Cordeiro Cavalcanti ◽  
Joyce Galvão de Souza ◽  
Filipe Jordão Pereira de Medeiros ◽  
João Carlos Taveira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Pathogenic Bacteria ◽  
Goat Milk ◽  
Salmonella Spp ◽  
Pasteurized Milk ◽  
Enterococcus Spp ◽  
Bacillus Spp ◽  
Thermotolerant Coliforms ◽  
Staphylococcus Spp

The sanitary evaluation of equipment and hands is fundamental to investigate the presence of pathogens in the dairy industry. Then, this study aims to evaluate the sanitization of equipment, workers’ hands, raw and pasteurized milk in goat milk dairies in the Cariri region, state of Paraíba.  Collected 32 samples of four dairies represented by letters A, B, C, and D. The followings contents were analyzed: mesophiles, total and thermotolerant coliforms, Escherichia coli, Staphylococcus aureus, Samonella spp. and Listeria monocytogenes in the reception tank, pasteurization tank, packing machine, package, wall, workers’ hand, and each dairy’s raw and pasteurized milk. After isolation, 84 colonies were confirmed by MALDI TOF. The indicator microorganisms presented variations for the workers’ hands, while A and B stayed within the patterns. For the equipment, only dairy B was within limits. They were out of the standard for mesophiles, total coliforms, and thermotolerant regarding raw and pasteurized milk. The microorganisms, the Enterobacteriaceae family presented a higher frequency, with 77.38%, and within this family, Escherichia coli, Klebsiella spp., and Enterobacter spp. were the most prevalent. Gram-positive corresponded to 22.62%, Bacillus spp., Staphylococcus spp., Enterococcus spp., and Macrococcus caseolyticus. Listeria monocytogenes and Salmonella spp. were not isolated. These demonstrate failures in goat milk processing with pathogenic bacteria in several dairy plants, indicating the need to adjust the product’s quality control.

scholarly journals Antimicrobial effects of spices and herbs essential oils

2009 ◽  
pp. 195-209 ◽  
Author(s):  
Marija Skrinjar ◽  
Nevena Nemet
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Food Additives ◽  
Inhibitory Effect ◽  
Natural Food ◽  
Garlic Mustard ◽  
Salmonella Spp ◽  
Food Preservatives ◽  
Bacteria And Fungi ◽  
Staphylococcus Spp

Spices and herbs have been used as food additives since ancient times, as flavouring agents but also as natural food preservatives. A number of spices shows antimicrobial activity against different types of microorganisms. This article gives a literature review of recent investigations considering antimicrobial activity of essential oils widely used spices and herbs, such as garlic, mustard, cinnamon, cumin, clove, bay, thyme, basil, oregano, pepper, ginger, sage, rosemary etc., against most common bacteria and fungi that contaminate food (Listeria spp., Staphylococcus spp., Salmonella spp., Escherichia spp., Pseudomonas spp., Aspergillus spp., Cladosporium spp. and many others). Antimicrobial activity depends on the type of spice or herb, type of food and microorganism, as well as on the chemical composition and content of extracts and essential oils. Summarizing results of different investigations, relative antimicrobial effectiveness can be made, and it shows that cinnamon, cloves and mustrad have very strong antimicrobial potential, cumin, oregano, sage, thyme and rosemary show medium inhibitory effect, and spices such as pepper and ginger have weak inhibitory effect.

scholarly journals Antimicrobial activity of some Bacillus spp. Separated from the soil

2019 ◽  
Vol 10 (2) ◽  
pp. 1460-1463
Author(s):  
Zhara Tareq Abdulhameed Baqqal ◽  
Rafal Mhaidi Younus ◽  
Saja Dhyaaldain Mustafa
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Effect Of Temperature ◽  
Good Growth ◽  
Candida Spp ◽  
Gram Positive ◽  
Bacillus Spp ◽  
Bacillus Genus ◽  
Bacteria And Fungi

In this study select two active isolate from nine isolates of local agricultural soil of Mosul, were collected from 10 sample of 6 different region depending on antimicrobial activity to some test bacteria and fungi, Identified as gram-positive rod-shaped bacteria and, biochemical test, the selected isolates belonged to Bacillus genus, gave signal B1 and B2 were effective against both pathogenic bacteria Gram-positive and Gram-negative, two isolates gave high inhibition zone about 23mm against Staphylococcus aureus whereas gave less inhibition zone against Escherichia .coli, Klebsiella spp, Aspergillus niger and Candida spp, 20, 16, Furthermore studied the effect of temperature on the growth of two isolates which gave good growth at 40-45 degree whereas there was no growth at low temperature 5°C. Furthermore studied the effect of salt on the growth of two isolates that gave good growth at concentration 3% NaCl; the two strains were analysed utilizing agar publishing way and cross streak method.

scholarly journals Estimation of microbiological propagation and antimicrobial traits of the frequently accessible flowers

2015 ◽  
Vol 4 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Marufa Sharmin ◽  
Kamal Kanta Das ◽  
Mrityunjoy Acharjee
Keyword(s):  
Pathogenic Bacteria ◽  
Antimicrobial Activities ◽  
Salmonella Spp ◽  
E Coli ◽  
Pseudomonas Spp ◽  
Bacillus Spp ◽  
Almost All ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Present study portrayed a complete microbiological profile of commonly available flowers including Rosa kordesii, Gladiolus hybrid, Acmella oleracea, Nyctanthes arbortristis and Pseudomussaenda flava which were randomly collected from Dhaka city, Bangladesh. The microbial contamination was quantified up to 108 cfu/g. Exploration of specific pathogenic bacteria was estimated within the range of 103 to 108 cfu/g of which Pseudomonas spp. was found in G. hybrid, A. oleracea and P. flava (~106 cfu/g), whereas Escherichia coli and Staphylococcal contamination was evident in almost all samples up to 108 cfu/g. The in vitro antimicrobial activities of the flower extracts were notable against most of the test bacteria. The ethanolic extracts of R. kordesii showed anti-bacterial activity against most of the bacteria except E. coli and Salmonella spp. G. hybrid extracts showed activity against Klebsiella spp. and Bacillus spp., A. oleracea against E. coli, Pseudomonas spp., Bacillus spp., Klebsiella spp., Staphylococcus spp. and Salmonella spp., P. flava against Pseudomonas spp. and Bacillus spp., and N. arbortristis against Bacillus spp. The methanol extracts of G. hybrid possessed activity against E. coli, Listeria spp. and Pseudomonas spp., N. arbortristis extracts against E. coli, Vibrio spp., Bacillus spp., Klebsiella spp. and Staphylococcus spp., P. flava extracts against E. coli. DOI: http://dx.doi.org/10.3329/sjm.v4i1.22756 Stamford Journal of Microbiology, Vol.4(1) 2014: 19-23

scholarly journals Antimicrobial potency assay of common antibiotics collected from different drug stores in Dhaka Metropolis

2015 ◽  
Vol 3 (1) ◽  
pp. 26-29 ◽  
Author(s):  
Biswajit Modak ◽  
Md Shahinur Rahman ◽  
Mohammad Shahid Gazi ◽  
Md Aftab Uddin ◽  
Tasmina Rahman
Keyword(s):  
Pathogenic Bacteria ◽  
Public Health Issue ◽  
Magic Bullet ◽  
Bacterial Strains ◽  
Salmonella Spp ◽  
Potency Assay ◽  
Antibiotic Susceptibility Pattern ◽  
Bacillus Spp ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Antibiotic, known as the magic bullet, was developed to control the growth of pathogenic bacteria. Efficacy of an antibiotic depends on its target site on bacteria. The current public health issue is the emergence of resistant bacterial strains against these drugs. The present study was undertaken to perform the routine antibiotic susceptibility pattern of eight laboratory isolates (Escherichia coli, Bacillus spp., Staphylococcus spp., Vibrio spp., Salmonella spp., Pseudomonas spp., Klebsiella spp. and Listeria spp.) against eleven different categories of antibiotics (Ciprofloxacin, Cephradine, Metronidazole, Cefuroxime, Cefixime, Levofloxacin, Amoxicillin, Azithromycin, Doxycycline, Erythromycin and Flucloxacillin) available in local markets of Dhaka metropolis. Among these antibiotics, Azithromycin, Ciprofloxacin, Cefuroxime, Levofloxacin and Doxycycline showed the highest potency against these bacteria. On the contrary, Amoxicillin and Metronidazole showed almost no potency. Erythromycin, Cephradine and Flucloxacillin gave variable effectivity against the tested isolates. These findings reveal that measures for prevention and containment of antimicrobial resistance are necessary in Bangladesh. DOI: http://dx.doi.org/10.3329/sjm.v3i1.22749 Stamford Journal of Microbiology, Vol.3(1) 2013: 26-29

scholarly journals Isolation of Multi-Drug Resistant Potential Pathogenic Bacteria From Blow Fly Collected From Different Areas of Dhaka City

2021 ◽  
Vol 49 (2) ◽  
pp. 205-214
Author(s):  
Tangin Akter ◽  
Samira Jahan ◽  
Sangita Ahmed ◽  
Shanjida Sultana ◽  
Shefali Begum
Keyword(s):  
Pathogenic Bacteria ◽  
Dhaka City ◽  
Blow Fly ◽  
Salmonella Spp ◽  
Blow Flies ◽  
Fish Market ◽  
Shigella Spp ◽  
Bacillus Spp ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

The research work was conducted for isolation and identification of potential pathogenic bacteria from blow fly (Calliphora spp.) and to investigate their antibiotic susceptibility. For this purpose, 20 blow flies were collected from 4 selected areas (Curzon Hall, Dhaka Medical College Hospital, Fruit Store and Fish Market) of Dhaka city. A total of 40 samples (20 from external surface and 20 from alimentary gut homogenates) were prepared from the blow flies. Among the total 20 flies, 3(15%) were collected from Curzon Hall and 5(12.5%) isolates were obtained from them; 6(30%) flies were collected from DMCH which had 11(27.5%) isolates; 5(25%) flies were collected from fruit stores and 10(25%) isolates were collected from there; 6(30%) flies were collected from fish market and 14(35%) isolates were collected from there. The isolated bacteria were presumptively identified as Escherichia coli, 12 (30%); Salmonella spp., 8 (20%); Shigella spp., 4(10%); Enterobacter spp., 3 (7.5%); Klebsiella spp., 2 (5%); Bacillus spp., 7 (17.5%) and Staphylococcus spp., 4 (10%) in number which were based on morphology as observed under microscope as well as cultural and biochemical properties. All of these isolates were resistant to various antibiotics. Bacterial susceptibility showed that E. coli isolates were highly resistant (66.6%) to Penicillin, Salmonella spp. mostly resistant (62.5%) to Penicillin and Tetracycline. Klebsiella spp. Isolates were 100% resistant to Penicillin and 100% sensitive to Ciprofloxacin, Imipenem and Tetracycline. In case of Shigella spp. and Enterobacter spp., 75% and 100% isolates were resistant to Penicillin respectively. Among the Staphylococcus spp. isolates, 75% were resistant to Ampicillin and Penicillin. In case of Bacillus spp. isolates, 57.2% were resistant to Penicillin and 100% sensitive to Imipenem. Antibiotic resistance of these species affect our human health also. This study demonstrated the potential of blow flies as a vector of various pathogenic microorganisms and a mode of transmission of antibiotic resistance. Bangladesh J. Zool. 49 (2): 205-214, 2021

scholarly journals Study on the existence of food born microflora and their drug resistant profile isolated from some fermented and non-fermented foods commonly available in Bangladesh

2019 ◽  
Vol 8 (1) ◽  
pp. 19-23
Author(s):  
Tasnuva Nusrat Jane ◽  
Nafisa Tabassum ◽  
Mrityunjoy Acharjee
Keyword(s):  
Pathogenic Bacteria ◽  
Fermented Food ◽  
Contamination Rate ◽  
Fermented Foods ◽  
Drug Resistant ◽  
Salmonella Spp ◽  
Viable Bacteria ◽  
Bacteria And Fungi ◽  
Staphylococcus Spp ◽  
Vibrio Spp

Present study depicted a complete microbiological profile of some popular fermented and non-fermented foods available in different food shops and restaurants in Dhaka city, Bangladesh. Furthermore, All the isolated strain were prepared to determine their survivability against some common synthetic drug through Kirby Bouer method. Total 60 samples of 20 categories (10 fermented and 10 non-fermented) unveiled the contaminating microbial flora up to 107 cfu/g including total viable bacteria and fungi in case of non-fermented food while the contamination rate was extremely low up to 105cfu/g in fermented food. Exploration of specific pathogenic bacteria was estimated such as E. coli, Salmonella spp, Vibrio spp., Staphylococcus spp. and Pseudomonas spp. within the range of 102 to 104cfu/g in non-fermented foods whereas only the Staphylococcus spp. was present in fermented food up to 103cfu/g. Among all the specific pathogens Salmonella spp., Vibrio spp. and Staphylococcus were significantly propagated in all categories of non-fermented samples up- to 104cfu/g. Total 17 available drugs were used against the identified bacterial strain. Most of the identified bacteria from fermented foods were found to be sensitive against commonly used antibiotics. Subsequently, five isolates from non-fermented foods were found to be 100% resistant against more than one antibiotic as multi drug resistant (MDR). Stamford Journal of Microbiology, Vol.8(1) 2018: 19-23

Characterization of Bacteria and Fungi Bioaerosol in the Indoor Air of selected Primary Schools in Malaysia

2011 ◽  
Vol 20 (6) ◽  
pp. 607-617 ◽  
Author(s):  
Nor Husna Mat Hussin ◽  
Lye Munn Sann ◽  
Mariana Nor Shamsudin ◽  
Zailina Hashim
Keyword(s):  
Indoor Air ◽  
Primary Schools ◽  
Average Concentration ◽  
Outdoor Air ◽  
Inadequate Ventilation ◽  
Bacillus Spp ◽  
Bacteria And Fungi ◽  
Staphylococcus Spp ◽  
Indoor And Outdoor

This study reports the types and concentrations of bacterial and fungal bioaerosols found in five randomly selected primary schools in Malaysia. Normal flora bacteria was the most frequently isolated bacteria including Staphylococcus spp., Pseudomonas spp. and Bacillus spp. Terribacillus spp. found in this study had never been reported before. The most frequently isolated fungal genera were Aspergillus, Penicillium, Fusarium, Rhizopus and Zygomycetes. The average concentration of bacteria in indoor and outdoor air were 1025 ± 612 CFU/m3 and 1473 ± 1261 CFU/m3, respectively, while the average concentration of fungal bioaerosol in indoor and outdoor air were 292 ± 83 CFU/m3 and 401 ± 235 CFU/m3, respectively. The percentages of bacterial and fungal samples that were within the American Conference of Industrial Hygenists (ACGIH) recommended levels were 44% and 33.8%, respectively. The ratio of indoor to outdoor fungi concentration was below 1.0, suggesting minimal indoor generative source for fungal bioaerosols. However, the ratio of indoor to outdoor bacteria concentration was approaching 1.0, suggesting the presence of potential internal generative source and inadequate ventilation. Building occupants might be one of the potential sources of bacteria in the indoor air as the bacteria concentrations without occupants were significantly lower than with occupants (p < 0.05).

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