scholarly journals In-vitro Cytotoxicity and Antioxidant Studies of Elaeocarpus serratus

1970 ◽  
Vol 2 (2) ◽  
pp. 86-90 ◽  
Author(s):  
Most Nazma Parvin ◽  
Shammy Sarwar ◽  
Sadia Afreen Chowdhury ◽  
Hasan Mohammed Zakaria ◽  
Naz Hasan Huda
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Methanolic Extract ◽  
Soluble Fraction ◽  
Reducing Power ◽  
Stem Bark ◽  
Scavenging Activity ◽  
Ic50 Value

The pet-ether soluble fraction (PEF), Carbon tetrachloride soluble fraction (CTSF) and Chloroform soluble fraction (CFSF) of the methanolic extract of  stem bark of Elaeocarpus serratus were investigated for cytotoxic activity using brine shrimp lethality bioassay .The methanolic extract was also evaluated for possible antioxidant activity using nitric oxide scavenging activity and reducing power assays. In Elaeocarpus serratus, the LC50 value were found to be 14.94, 0.831 & 3.288 μg/mL in pet-ether soluble fraction (PEF), Carbon tetrachloride soluble fraction (CTSF) and Chloroform soluble fraction (CFSF) of the methanolic extract respectively. The extract showed significant antioxidant activity in nitric oxide scavenging activity and reducing power assay. In nitric oxide scavenging activity, the IC50 value of Elaeocarpus serratus extract was 89.325μg/mL while the IC50 value of ascorbic acid was 47.684μg/mL. It was found that scavenging of nitric oxide by the extract was concentration dependent.  Key words: Elaeocarpus serratus; Cytotoxicity; Antioxidant; Reducing power.DOI: 10.3329/sjps.v2i2.5829Stamford Journal of Pharmaceutical Sciences Vol.2(2) 2009: 86-90

Investigation of Oral Subacute Toxicity and In-Vitro Antioxidant Activity of Standardized Methanolic Extract of Quercus serrata Leaves

2020 ◽  
Vol 16 ◽  
Author(s):  
Maibam Beebina Chanu ◽  
Biseshwori Thongam ◽  
Khumukcham Nongalleima ◽  
Hans Raj Bhat ◽  
Surajit Kumar Ghosh ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Biochemical Parameters ◽  
Methanolic Extract ◽  
Reducing Power ◽  
Quercus Serrata ◽  
Control Group ◽  
Blood Cell Count ◽  
Toxicological Profile

Background: Quercus serrata Murray leaves have been used traditionally in the treatment of diabetes, dysmenorrhoea, inflammation and urinary tract infection. So, far no study had been reported on the toxicological profile and antioxidant properties of the plant. Objective: The present study was aimed to investigate the in-vivo toxicological profile and in-vitro antioxidant activities of the methanolic extract of standardized Quercus serrata leaves. Methods: Per-oral sub-acute toxicity study was performed in rats using three dose levels (200, 400 and 800 mg/kg b.w.) of the extract for 28-days. Control group received gum acacia suspended in water. Bodyweight was measured weekly. Biochemical parameters were analysed using the serum, the blood-cell count was done using whole blood. Pathological changes were also checked in highly perfused tissues. Further, in-vitro reducing power assay, nitric oxide scavenging assay, DPPH free-radical scavenging assay were performed to check the antioxidant activity of the extract. Results: There were no significant alterations in the blood-cell count and biochemical parameters analysed in the treatment group when compared with the normal control. Histopathology study of liver, kidney, pancreas, heart and brain revealed normal cellular architecture in the treatment groups alike the control group animals. Quercus serrata also showed a significant reduction of DPPH with IC50 4.48±0.254 µg/mL, in-vitro reducing power activity with IC50121.65±0.320 µg/mL and nitric oxide scavenging activity IC50 106.43±0.338 µg/mL. Conclusion: The above study showed that standardized methanolic extract of Quercus serrata leaves was safe after subacute oral administration in rats and has good antioxidant potential.

scholarly journals Evaluation of antioxidant activity of unpurified and purified datura seed

2021 ◽  
Vol 7 (1) ◽  
pp. 030-035
Author(s):  
Umang Haribhai Gajjar ◽  
Niralee Kalpeshkumar Velhal ◽  
Hetvi Girdharbhai Parikh ◽  
Jayrajsinh Bhartbhai Parmar ◽  
Yash Bhut ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Reducing Power ◽  
Datura Stramonium ◽  
Cow Milk ◽  
Ferric Ion ◽  
Scavenging Activity ◽  
Ic50 Value ◽  
Cow Urine ◽  
Assay Result

Aim: Evaluation of effect of shodhana process of datura seed (Datura stramonium) antioxidant activity. Methods: The datura was purified with two different processes viz. cow’s urine, and cow’s milk by Dolayantra method. Antioxidant activity was evaluated by in-vitro 1,1-Diphenyl-2-picryl hydrazyl (DPPH) radicals scavenging activity and Ferric reducing power ability (FRPA) assay. Result: cow urine purified datura (CUD) showed a significant effect in inhibiting DPPH, reaching up to 85.96% at concentration 1000 mcg/ml and its IC50 was 314.3 mcg/ml while Unpurified Datura Seed (UD) reaching up to 86.06% at concentration 1000 mcg/ml and its IC50 was 171.73 mcg/ml. The Cow Milk Purified Datura Seed (CMD) extract reach 69.72% at concentration 1000 mcg/ml and its IC50 value was 640. The IC50 value of Ascorbic acid was 23.35 mcg/ml. CUD showed a significant effect in reduction of ferric ion, reaching up to 85.59% reduction at concentration 1000 mcg/ml and its EC50 was 304.46 mcg/ml while UD reaching up to 86.11% at concentration 1000 mcg/ml and its EC50 was 280.28 mcg/ml. CMD extract reach 52.28% at concentration 1000 mcg/ml and its EC50 value was 1091.3mcg/ml.

scholarly journals Antioxidant Activity Profiling of Acetonic Extract of Jamun (Syzygium cumini L.) Seeds in Different In-Vitro Models

2020 ◽  
Vol 12 (1) ◽  
pp. 3-8
Author(s):  
Neha Yadav ◽  
Ajay Pal ◽  
Sonam Sihag ◽  
Nagesh C.R
Keyword(s):  
Nitric Oxide ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Metal Chelation ◽  
Syzygium Cumini

Background: Syzygium cumini L., commonly known as Jamun, black-plum, and Indian blackberry, is one of the most widely distributed trees in India with booming medical benefits and possesses antioxidant, anticancer and anti-diabetic properties. It belongs to the family Myrtaceae. Despite countless phytochemicals, seeds are not consumed and are the waste part of Jamun fruit. Objective: The objective of this study was to evaluate the antioxidant capacity of phenolics from Jamun seeds against a bundle of oxidant moieties. Methods: The 50% acetone extract of Jamun seeds was investigated for in-vitro antioxidant profiling. Assays include free radical scavenging activity, metal chelation activity, hydroxyl radical scavenging activity, hydrogen peroxide scavenging activity, total antioxidant activity, total reducing power, nitric oxide scavenging activity, and lipid peroxidation inhibition activity. Results: The extract depicted maximum DPPH radical scavenging activity followed by ABTS radical scavenging activity. Hefty metal chelation and nitric oxide scavenging activity were recorded while lipid peroxidation, H2O2, and OH- scavenging activity was intermediate. Conclusion: Jamun seed showed ample antioxidant activity and certifies that it is the right candidate for exploitation as a source of natural antioxidants to counteract autoxidation-induced pathologies or diseases.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT ACTIVITY OF THE METHANOLIC EXTRACT OF LINDERNIA RUELLIOIDES (COLSM.) PENNELL

2020 ◽  
pp. 141-146
Author(s):  
IMTILEMLA A ◽  
VICKY BAREH ◽  
SAMIA BEGAM BARBHUIYA ◽  
LALZIKPUII SAILO
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Reducing Power ◽  
Fluorescence Analysis ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Reducing Power Assay ◽  
Dpph Scavenging ◽  
In Vitro Antioxidant Activity

Objective: The objective of the study was collection of plant materials, Extraction of phytoconstituents using a different solvent, to carry out preliminary phytochemical screening of different extracted solvent, to perform fluorescence analysis, to estimate the proximate composition of the leaves Lindernia ruellioides (Colsm.) Pennell, and to determine the presence of in vitro anti-oxidant of the methanolic extract of the plant. Method: Preliminary phytochemical screening of the methanolic extract of Lindernia ruellioides (Colsm.) Pennell, estimation of proximate composition of the leaves, fluorescence analysis, total phenolic content, total flavonoids content, and in vitro antioxidant activity of the methanol extract (DPPH scavenging activity, reducing power assay, and nitric oxide scavenging activity). Results: The result of phytochemical screening of methanolic extract of Lindernia ruellioides (Colsm.) Pennell contents the presence of amino acid, flavonoids, tannins, steroids, and triterpenoids. The moisture content and Ash value were found to be appropriate and the in vitro antioxidant activity of the methanolic extract showed potential antioxidant activity in terms of DPPH scavenging activity, reducing power assay, and nitric oxide scavenging activity. Conclusion: The work presented here suggests that the methanolic extract of Lindernia ruellioides (Colsm.) Pennell possesses potential antioxidant activity.

scholarly journals Determination of in vitro antioxidant activity of the leaves extracts of Ehretia pubescens

2020 ◽  
Vol 11 (4) ◽  
pp. 6262-6267
Author(s):  
Krishnamoorthy Meenakumari ◽  
Giridharan Bupesh ◽  
Mayur Mausoom Phukan
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Biological Properties ◽  
Scavenging Activity ◽  
Reducing Power Assay

The foods from plants were known to ensure against degenerative diseases and maturing because of their antioxidant activitycredited to their high content. Information on antioxidant activity of Indian medicinal plant is abundant. To the best of our knowledge, biological properties have not been accounted in the literature for this species of . As a point, this is the first results to assess the anti-oxidant activity of the plant which belongs to the family . The antioxidant activity of Methanol, , Ethyl acetate and Aqueous extracts of E. was determined using the DPPH free radical scavenging activity, ABTS radical scavenging activity and reducing power assay. The DPPH scavenging activity showed higher activity observed in extract (63%) of E. than (54%), (44%) and aqueous (30%). the ABTS assay inhibition in extract (58%) than (43%), (38%) and aqueous (32%) extracts. The reducing power assay of different extracts was increased in extract (54%) than (40%), (34%) and aqueous (28%) extracts. Overall, the and ethyl acetate extract had higher antioxidant properties than other extract. However, in this study, extracts exhibit great potential for antioxidant activity and may be useful for their nutritional and medicinal functions.

scholarly journals Evaluation of Free Radical Scavenging and Polyphenolic Contents of Bark of Pterygota alata Roxb

2014 ◽  
Vol 6 (3) ◽  
pp. 543-552 ◽  
Author(s):  
N. Jahan ◽  
M. S. Parvin ◽  
A. Khan ◽  
N. Das ◽  
M. S. Islam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Soluble Fraction ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Deciduous Tree ◽  
Total Phenolic ◽  
Phenolic And Flavonoid

Pterygota alata is a large deciduous tree of Malvaceae family. The present study was designed to evaluate in vitro antioxidant activity of stem bark of the plant. Ferric reducing power (FRP) test, 1, 1- diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging test and total antioxidant capacity (TAC) assay were used to detect the antioxidant activity. Total phenolic and flavonoid contents were also determined as they are well known phytochemicals with antioxidant property. The bark of the plant showed efficient reducing power as well as free radical scavenging property (IC50 values 52.25 - 172.05). The bark also found rich in total phenolic and flavonoid content. The highest amount of total phenolic content was found in chloroform soluble fraction (29.898 µg/mL) followed by ethyl acetate soluble fraction (15.88 µg/mL). The highest content of total flavonoid also detected in chloroform soluble fraction (107.56 µg/mL) but followed by crude ethanol extract (98.66 µg/mL). Overall, the bark of the plant possesses significant antioxidant activity, therefore can be used as a good natural source of antioxidant. © 2014 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. doi: http://dx.doi.org/10.3329/jsr.v6i3.17586 J. Sci. Res. 6 (3), 543-552 (2014)

scholarly journals Biological Activities of the Polysaccharides Produced in Submerged Culture of Two EdiblePleurotus ostreatusMushrooms

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Emanuel Vamanu
Keyword(s):  
Antioxidant Activity ◽  
Pleurotus Ostreatus ◽  
Submerged Culture ◽  
Biological Activities ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Carbohydrate Analysis ◽  
Chelating Activity ◽  
Batch System

Exopolysaccharides (EPS) and internal (intracellular) polysaccharides (IPS) obtained from thePleurotus ostreatusM2191 and PBS281009 cultivated using the batch system revealed an average of between 0.1–2 (EPS) and 0.07–1.5 g/L/day (IPS). The carbohydrate analysis revealed that the polysaccharides comprised 87–89% EPS and 68–74% IPS. The investigation of antioxidant activityin vitrorevealed a good antioxidant potential, particularly for the IPS and EPS isolated from PBS281009, as proved by the EC50value for DPPH, ABTS scavenging activity, reducing power, and iron chelating activity.

In vitro Antioxidant Capacities of Two Benzonaphthoxanthenones: Ohioensins F and G, Isolated from the Antarctic Moss Polytrichastrum alpinum

2009 ◽  
Vol 64 (3-4) ◽  
pp. 197-200 ◽  
Author(s):  
Hari Datta Bhattarai ◽  
Babita Paudel ◽  
Hong Kum Lee ◽  
Hyuncheol Oh ◽  
Joung Han Yim
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Commercial Application ◽  
Moss Species ◽  
Antioxidant Capacities ◽  
Antioxidant Agents ◽  
Antarctic Moss

Antioxidant agents against reactive oxygen species can be used for several cosmetic and medicinal applications. This study’s objective was to evaluate the antioxidant activities of Polytrichastrum alpinum (Hedw.) G. L. Sm. (Polytrichaceae), an Antarctic moss species collected from King George Island (Antarctica). The identifi cation of the moss species was performed on the basis of morphological characteristics and molecular sequencing of the 18S rRNA gene. Two benzonaphthoxanthenones: ohioensins F and G, were isolated from the extract after several chromatographic procedures. The various in vitro antioxidant capacities of a methanolic extract of P. alpinum and the isolated compounds were evaluated by analyzing the scavenging capacities of free radicals of 2,2-azino-bis(3-ethylbenzthiazoline- 6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH), the total phenol assay with Folin-Ciocalteu reagent, the ferric ion (Fe3+) reducing power and the nitric oxide (NO) scavenging activity and compared to those of commercial standards for each assay. The experimental data showed that even the crude extract of P. alpinum exhibited potent antioxidant activity. The antioxidant activity was increased two- to seven-fold for the purified compounds. The antioxidant activities of both purified compounds were found to be more or less the same in all experiments. However, the obtained data showed that the Fe3+ reducing power of the purified compounds and crude methanolic extract was almost the same suggesting the presence of other stronger reducing agents in the methanolic extract which could not be isolated in the present experiment. Therefore, further work on the isolation of these stronger antioxidant agents from this moss specimen of the extreme environment is warranted. Developments of laboratory mass culture techniques are anticipated to achieve bulk production of the active constituents for commercial application.

scholarly journals Studies on In Vitro Antioxidant Activity of Methanolic Extract and Fractions of Ficus Hispida Lin. Fruits

2016 ◽  
Vol 8 (3) ◽  
pp. 371-380 ◽  
Author(s):  
M. S. Hossain ◽  
S. Parvin ◽  
S. Dutta ◽  
M. S. I. Mahbub ◽  
M. E. Islam
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Traditional Use ◽  
Total Antioxidant ◽  
Ficus Hispida

The present study was designed to confirm the traditional use of the fruits of Ficus hispida Linn. (Moraceae) as an antioxidant agent. Fruits of the plant extracted with methanol and crude methanol extract (CME) were further fractionated with n-hexane, chloroform, and ethyl acetate. All the fractions, n-hexane (NHF), chloroform (CHF), ethyl acetate (EAF), aqueous (AQF) and CME were preliminary screened for in vitro antioxidant activity and total phenolic and total flavonoid content. In DPPH radical scavenging assay, CME exhibited highest scavenging activity (IC50 = 11.20 µg/mL) as compared to other fractions. In this assay, IC50 of reference standard BHT was 5.10 µg/mL. The reducing power of the samples was in the order as AQF > CME > CHF > EAF > NHF. The results for hydrogen peroxide scavenging activity indicated that CME, EAF and AQF had almost the same scavenging activity except NHF. Total antioxidant capacity of CME and other fractions were ranked as CHF > AQF > CME > EAF > NHF.  In the assay of antioxidant constituents (total phenol and total flavonoids content), the CME had highest phenolic and flavonoids content. The results indicate that Ficus hispida fruits could be considered as a potential source of natural antioxidant.

scholarly journals Ganoderma lucidum Metanolik Ekstraktının Fitotoksik Etkilerinin ve Antioksidan Potansiyelinin Araştırılması

2016 ◽  
Vol 4 (3) ◽  
pp. 163 ◽  
Author(s):  
Fuat Bozok ◽  
Tülin Eker ◽  
Gökhan Sezer ◽  
Adnan Bozdoğan ◽  
Hasan Hüseyin Doğan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Ganoderma Lucidum ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Phytotoxic Effect ◽  
Dpph Scavenging Activity ◽  
Allelopathic Effects ◽  
Dpph Scavenging

The aim of this study is to reveal allelopathic effects and antioxidant activities of methanolic extract of Ganoderma lucidum in Zorkun plateau (Osmaniye). It was determined that methanolic extract of G. lucidum has significantly phytotoxic effect by increasing the doses (1, 2, 4, 8 mg/mL) on Hordeum vulgare and Triticum aestivum. Total phenol and flavonoid amounts of G. lucidum were 114.55 mg/kg and 8.95 mg/kg, respectively. 2,2-diphenyl-1-picrylhydrazyl (DPPH), scavenging activity (60%, 91% and 92%), reducing power (0.83, 1.43 and 2.23 Abs), and nitric oxide (NO) scavenging activity (48%, 55% and 70%) of this mushroom at the different concentrations (1.25, 2.5 and 5 mg/mL) were determined, respectively. However, H2O2 scavenging activity of the different concentrations (0.0156-0.125 mg/mL) was found as 18%, 56%, 60% and 86%, respectively. Based on these findings, it can suggest that G. lucidum methanolic extract has significantly phytotoxic effect and antioxidant activity. The present study is the first report on the phytotoxic effects and antioxidant activities of G. lucidum which consumed and collected from Amanos Mountains.

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