scholarly journals PENGARUH METODE EKSTRAKSI MINYAK PUPA ULAT JERMAN TERHADAP KOMPOSISI KIMIA

BUANA SAINS ◽  
2018 ◽  
Vol 18 (1) ◽  
pp. 67
Author(s):  
Erik Priyo Santoso ◽  
Farida Kusuma Astuti

The aim of this research is to know the extraction method of caterpillar pupa without detrimental to chemical composition to know the amount of pupa production of German caterpillar with two extraction methods per 100 grams of pupa To know the content of dry matter, organic matter, crude protein, crude fat, crude fiber and amino acid lysin, methionine, and vitamin E pupa oil of German caterpillar. The study was conducted from February to April 2017, at the Lab. Central University Tribhuwana Tunggadewi. Oil extraction analysis was conducted in the chemistry laboratory of Tribhuwana Tunggadewi University and chemistry laboratory of Poltek Malang. German caterpillar research material, German caterpillar pupa, pupa caterpillar germany extracted by maceration and sokletasi. German caterpillar pupa is obtained from German caterpillar breeders in Amadanom, Dampit, Malang Regency. This research uses two different extraction methods, namely cold extraction method (maseration method) and using heat method (soxhlet method). The conclusions of both research methods are known that the quantity of German caterpillar yield using soxhlet extraction method is more than the maceration extraction method. However, for the quality of the two methods it is still necessary to do further analysis to know the exact comparison of the two methods.

1975 ◽  
Vol 55 (4) ◽  
pp. 949-954 ◽  
Author(s):  
M. C. J. VAN ADRICHEM ◽  
J. N. TINGLE

The effects of spring-applied nitrogen (0, 56, 112 and 224 kg/ha) and phosphorus (0 and 27.4 kg/ha) on the dry matter (DM) yield and forage quality of successive harvests of meadow foxtail (Alopecurus pratensis L.) were investigated. Nitrogen increased DM yield, crude protein, Cu, K and Zn contents and decreased P, Ca, Mg and Mn contents. Application of P in combination with N increased K content in the first cut and arrested the decline of P content due to N application in all cuts. The levels of dry matter digestibility and Cu declined in successive cuts whereas Mn content increased. At low N rates, Ca and Mg contents increased as the season advanced.


2013 ◽  
Vol 59 (4) ◽  
pp. 167-177
Author(s):  
Miriam Kizeková ◽  
Ján Tomaškin ◽  
Jozef Čunderlík ◽  
Ľubica Jančová ◽  
Janka Martincová

Abstract This study highlights the effect of drought and ambient temperature on performance and herbage quality of legume monocultures and grass-legume mixtures. In a field experiment, the total dry matter yield, seasonal pattern of dry matter yield distribution, content of crude protein and crude fibre of monocultures of red clover and alfalfa and grass-legume mixtures were investigated during two consecutive dry years (2011-2012). Alfalfa cultivars Kamila and Tereza grown as monocultures or as mixtures with Festulolium braunii (cultivar Achilles) outperformed the red clover cultivars Fresko and Veles and provided a well-balanced total and seasonal dry matter yield during both years. Across all experimental years, crude protein content was significantly higher at alfalfa monocultures and mixture when compared with clover monocultures (P < 0.05). However, considerable lower content of crude fibre at clover monocultures in comparison with alfalfa ones was found. Responses of nutritive parameters of both legume species to weather variables were different. Crude protein content in red clover was independent of rainfall and temperature. In contrast, the crude fibre content correlated with temperature whereby the alfalfa monocultures showed stronger correlations (P < 0.05) than red clover monocultures.


2017 ◽  
Vol 6 (1) ◽  
pp. 26 ◽  
Author(s):  
Aqni Hanifa

<div class="Section1"><p class="Style1"><em>An experiment was conducted to study blood profile, milk yield and </em><em>liveweight gain of dairy cows as affected by dietary different quality of diets. The </em><em>experiment used </em><em>15 </em><em>lactating PFH were assigned into three treatments of diets and</em></p></div><em><br clear="all" /> </em><p class="Style1"><em> </em><em>five replication, three treatments of diets were </em><em>: </em><em>1) T1 (CP </em><em>12% dan TDN 65%); 2) T2 (CP 14% dan TDN 70%) dan 3) T3 (CP 16% dan TDN 75%).</em></p><p class="Style1"><em>The results of this experiment showed that the average of dry matter (DM) intake on Tl, T2 and T3 treatments were </em><em>8,59; </em><em>10,03 and </em><em>10,94 </em><em>kg/d, respectively </em><em>(P&lt;0,01). The average of crude protein intake (CP) on T1, </em><em>T2 and T3 treatments were 1,06; 1,44 and 1,71 kg/d (P&lt;0,01), also the average of TDN intake on T1, T2 and T3 treatments were 5,52; 6,86 and 8,02 kg/d (P&lt;0,01). The average of Hemoglobin (Hb) concentration on T1, T2 and T3 treatments were 10,21: 11,18 and 10,44 g/dl (P&gt;0,05), also the average of eritrosit value on TI, T2 and T3 treatments were 3,37; 3,73 and 3,48 million/mm<sup>3;</sup>, respectively (P&gt;0,05). The average of milk yield on T1, T2 and T3 treatments were 8,12; 11,29 and 13,42 l/d (P&lt;0,05). The average of liveweight gain on TI, T2 and T3 treatments were 81,66; 117,71 and 320,00 g/d (P&gt;0,05). </em></p><p class="Style1"><em>The conclusions of this research was the highest level of CP and </em><em>"1'DN </em><em>in </em><em>diets (CP 16% and </em><em>TDN 75%) showed that feed intake (DM, CP and TDN) and milk yield gave the best than other treatments. Different dietary quality of diets altered significant on feed intake (DM, CP and TDN) and milk yield, but the treatments did not affect on liveweight gain, Fib and eritrosit concentration.</em></p><p><em> </em></p><em>Key words :     diets quality, feed intake, blood profile, milk yield, liveweight gain, dairy cows</em>


2014 ◽  
Vol 68 (5) ◽  
Author(s):  
Hafizah Mohd Hadzri ◽  
Mohd Azizi Che Yunus ◽  
Salman Zhari ◽  
Fahim Rithwan

The effects of different types of solvents and extraction method were investigated to determine the presence of antioxidant contents and activity from the P. niruri plant. The aim of this study is to determine which extraction method will give higher natural antioxidant contents and antioxidant activity. The content of natural antioxidant and antioxidant activity were analysed by total phenolic content (TPC), total flavonoid content (TFC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity assay. The results showed that extracts from a supercritical fluid extraction (SFE) method without the addition of modifier showed the highest content of total phenolic (187.66 mg GAE/ g) and flavonoid (1100.93 mg QE/g) in P. niruri compared to the other methods of extraction with different type of solvents. The extract of P. niruri from different extraction methods showed antioxidant activity on DPPH radical scavenging assay. The soxhlet extraction method by methanol showed the lowest IC50 compared to the other methods of extraction. The results revealed that P. niruri extracts had different content of antioxidant and antioxidant activity. The solvent polarity and different methods of extraction play significant roles in determining the most suitable method for production of antioxidant contents and antioxidant activity from P. niruri extracts.


1980 ◽  
Vol 60 (3) ◽  
pp. 807-811 ◽  
Author(s):  
R. S. FULKERSON

Midas marrowstem kale (Brassica oleracea L.) was grown in different row width associations with United 106 corn (Zea maize L.) in two studies and ensiled in different moisture blends with corn stover in another. Highest dry matter yields were obtained where a single row of kale was grown at 30 cm to the side of a corn row. This combination also provided the lowest moisture content feed and the highest in vitro digestibility and crude protein content. Changing the corn row width had no significant effect upon yield, plant height, in vitro digestibility, kale leaf or corn ear content. Blending kale with corn stover to provide a silage of about 70% moisture increased the digestibility and protein content of the feed and provided a silage that kept well in storage.


2011 ◽  
Vol 51 (No. 6) ◽  
pp. 271-277 ◽  
Author(s):  
W. OPITZ VON BOBERFELD ◽  
K. BANZHAF ◽  
F. HRABE ◽  
J. SKLADANKA ◽  
S. KOZLOWSKI ◽  
...  

In addition to the results published in the first communication (Opitz von Boberfeld et al., 2006) this paper presents crude protein, energy (ME) and ergosterol concentrations of autumn saved herbage at different sites in Central Germany, Poland, Hungary and the Czech Republic. Within these sites, the influence of the factors pre-utilisation and winter harvest date was tested over three consecutive years. Related to the different climatic conditions of the sites, crude protein concentrations of the growths pre-utilised in July varied from 149 g/kg in November to 134 g/kg of dry matter (DM) in January. The influence of climatic conditions was different on each location and varied depending on the year. Generally, the consistent effect of the factor &ldquo;site&rdquo; related to altitude could not be observed. While the energy concentrations decreased with advancing winter and partly reached the values below 6 MJ&nbsp;ME/kg of DM in January, the ergosterol concentrations increased. The results demonstrate that under Central European conditions autumn saved herbage, pre-utilised in July, could provide adequate quantity and quality for suckler cows until December. Afterwards, the utilisation of preserved forages becomes essential. &nbsp;


2018 ◽  
Vol 58 (2) ◽  
pp. 376 ◽  
Author(s):  
Marta Bykowska ◽  
Marek Stanisz ◽  
Agnieszka Ludwiczak ◽  
Joanna Składanowska ◽  
Piotr Ślósarz

The quality of three muscles (supraspinatus, longissimus, semimembranosus) were studied in a group of 12 farmed fallow deer bucks (Dama dama) aged 28 months. The aim was to compare the selected quality traits of these muscles and to assess the extent to which these traits change after 14 days of being stored in vacuum packaging. Both the pH and the temperature were significantly affected by the muscle (P < 0.0001) and the time of storage (P < 0.0001). The supraspinatus muscle had the highest initial and final pH (pH0.5 h = 6.95; pH15d = 5.93), while m. semimembranosus showed the greatest drop in temperature (by 37.5°C) 24 h postmortem. The colour parameters differed among muscles. Only lightness (L*) was not influenced by the duration of storage (P = 0.081). The 14-day storage in a vacuum bag caused a decrease in the drip loss (P = 0.002), free water (%; P = 0.001), free water (cm2; P < 0.0001) and a cooking loss (P = 0.050). The duration of time in storage caused an increase in the dry-matter (P = 0.049) and crude-protein (P = 0.044) contents and a decrease of the water to protein ratio (W : CP; P = 0.014). There was a significant effect of muscle on the dry-matter (P < 0.0001) and crude-protein (P < 0.001) contents and W : CP (P < 0.0001). The highest contents of dry matter (P ≤ 0.01) and crude protein (P ≤ 0.01) were found in m. longissimus. The highest W : CP was observed in m. supraspinatus (W : CP24 h = 4.02; W/CP15d = 3.92). The results indicated a different technological quality of the analysed muscles, and, thus, a need to further explore the background of these differences in the early postmortem period and after meat maturation.


2015 ◽  
Vol 3 (3) ◽  
pp. 398-401
Author(s):  
Ranganathan Kapilan

Extraction of DNA is very important nowadays in bio-molecular researches. Extracted DNA should be purified and the quality of DNA should also be very high. The objective of the study was to develop a simple efficient method to isolate DNA from the rice varieties in an open laboratory environment, and to eliminate the usage of expensive chemicals and tools. The DNA extraction methods developed by the DNeasy plant kit method supplied by QIAGEN, Cheng et al., Doyle et al. and Michiels et al. were applied to five different rice varieties grown in different parts of Sri Lanka. Based on the quantity and quality of the extracted DNA tested by measuring the absorbance of DNA at 260 nm using Nanodrop® ND-1000 spectrophotometer and measuring the ratio of A260 / A280 and gel running on agarose, the efficiency of the extraction method chosen varied among rice varieties. Among the methods used, the methods introduced by DNeasy plant kit method supplied by QIAGEN and Cheng et al, yielded good and amplifiable quality DNA with satisfactory concentration for all the rice varieties tested. Therefore the modified method of Cheng et al, 1987 could be used to extract DNA from rice varieties instead of the commercially available expensive and hazardous DNeasy plant kit method supplied by QIAGEN.Int J Appl Sci Biotechnol, Vol 3(3): 398-401


2004 ◽  
Vol 84 (2) ◽  
pp. 529-531 ◽  
Author(s):  
Shi-kui Dong, Yuan Jiang ◽  
Quan-ru Liu ◽  
Xiao-xia Huang

Herbage yield increased with elevation from 2200 to 2600 m and then declined until 2800 m. Altitude did not affect herbage fat, acid detergent fibre, calcium or sulphur concentrations, but did influence herbage organic matter, crude protein and phosphorus concentrations. It is recommended that livestock should be rotationally grazed along an altitudinal gradient on Xiaowutai Mountain to meet their dry matter (DM) and nutrients requirements in summer grazing systems. Key words: Herbage biomass, crude protein, acid detergent fibre, minerals


2018 ◽  
Vol 53 (9) ◽  
pp. 1045-1052
Author(s):  
Mateus Merlo Coelho ◽  
Lúcio Carlos Gonçalves ◽  
José Avelino Santos Rodrigues ◽  
Kelly Moura Keller ◽  
Gustavo Vinícius de Souza dos Anjos ◽  
...  

Abstract: The objective of this work was to evaluate the effects of re-ensiling and bacterial inoculation on the quality of corn silage. The experiment was carried out in a 2x2 factorial design with or without inoculant (association of Lactobacillus plantarum and Propionibacterium acidipropionici), and with re-ensiling after 36 hours of aerobic exposure or only ensiling of the whole plant of 'BRS 1055' corn. The fermentative quality, nutritional parameters, dry matter losses, aerobic stability, and microbiological counts of silages were evaluated. Re-ensiling caused an increase of pH and in acetic acid and propionic acid concentrations, as well as in the dry matter (DM), crude protein, neutral detergent fiber, and neutral detergent fiber crude protein contents. Conversely, there was a reduction in the nonfiber carbohydrates concentration and in in vitro dry matter digestibility for the re-ensiled material. All changes were explained by the higher-effluent production and DM loss of re-ensiled material that was subjected to two compactions. Microbiology was not altered by the treatments. The use of inoculant altered ash content, but it did not influence other parameters. In contrast, re-ensiling after 36 hours of aerobic exposure caused a reduction in the nutritive value of corn silage and accentuated the DM losses.


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