scholarly journals Ozonized water used as complementary therapy for stomatitis in Salvator merianae – case report

2021 ◽  
Vol 10 (11) ◽  
pp. e280101119550
Author(s):  
Lucas Micael Freire Pereira ◽  
Fabiano Rocha Prazeres Júnior ◽  
João Vitor de Oliveira Gurgel ◽  
Vanessa Silva Santana ◽  
Leonardo Lomba Mayer ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Low Cost ◽  
Complementary Therapy ◽  
Ex Situ ◽  
Tissue Cell ◽  
Wild Animals ◽  
Ozone Therapy ◽  
Black And White ◽  
The Family ◽  
Stimulation Of

The Argentine black and white tegu (Salvator merianae) is a reptile belonging to the family Teiidae. Compared with other families, teiids are morphologically similar. However, their sizes vary considerably, with S. merianae being the largest endemic lizard in South America. The characteristics, habitat, and habits of S. merianae need to be investigated, to provide adequate ex situ adaptation opportunities and avoid accidents in the pet industry. One advancing complementary therapy is the use of ozone, which has potent antimicrobial action when applied topically, due to the oxidizing and healing effects resulting from the stimulation of tissue cell proliferation and remodeling. To generate information concerning ozone therapy, the treatment of stomatitis caused by Portland cement in an Argentine black and white tegu using ozonized water as a complementary therapy is reported. Fluid and antibiotic therapies (enrofloxacin, butorphanol, meloxicam, and sucralfate) were introduced, along with the daily rinsing of the oral cavity with freshly prepared ozonized water. No toxic effects relating to ozone use were observed, and its topical use as a complementary therapy proved to be low cost and highly efficient. These findings demonstrate the need to obtain knowledge concerning this therapy and promote its use in wild animals.      

scholarly journals P62.09 PLGF Regulates Stimulation of Cell Proliferation and Glycolysis of Lung Adenocarcinoma Through Wnt/β-catenin Pathway

2021 ◽  
Vol 16 (3) ◽  
pp. S550-S551
Author(s):  
B. Han ◽  
W. Zhang ◽  
Y. Zhang ◽  
F. Qian ◽  
W. Zhou ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Lung Adenocarcinoma ◽  
Stimulation Of

scholarly journals Polyamine-modulated c-Myc expression in normal intestinal epithelial cells regulates p21Cip1 transcription through a proximal promoter region

2006 ◽  
Vol 398 (2) ◽  
pp. 257-267 ◽  
Author(s):  
Lan Liu ◽  
Xin Guo ◽  
Jaladanki N. Rao ◽  
Tongtong Zou ◽  
Bernard S. Marasa ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Transcriptional Repression ◽  
Critical Role ◽  
Ectopic Expression ◽  
Proximal Region ◽  
Proximal Promoter ◽  
Transcriptional Level ◽  
Intestinal Epithelial ◽  
Major Player ◽  
Stimulation Of

Maintenance of intestinal mucosal epithelial integrity requires cellular polyamines that regulate expression of various genes involved in cell proliferation, growth arrest and apoptosis. Our previous studies have shown that polyamines are essential for expression of the c-myc gene and that polyamine-induced c-Myc plays a critical role in stimulation of normal IEC (intestinal epithelial cell) proliferation, but the exact downstream targets of induced c-Myc are still unclear. The p21Cip1 protein is a major player in cell cycle control, which is primarily regulated at the transcriptional level. The current study was designed to determine whether induced c-Myc stimulates normal IEC proliferation by repressing p21Cip1 transcription following up-regulation of polyamines. Overexpression of the ODC (ornithine decarboxylase) gene increased levels of cellular polyamines, induced c-Myc expression and inhibited p21Cip1 transcription, as indicated by repression of p21Cip1 promoter activity and a decrease in p21Cip1 protein levels. In contrast, depletion of cellular polyamines by inhibiting ODC enzyme activity with α-difluoromethylornithine decreased c-Myc, but increased p21Cip1 transcription. Ectopic expression of wild-type c-myc not only inhibited basal levels of p21Cip1 transcription in control cells, but also prevented increased p21Cip1 in polyamine-deficient cells. Experiments using different p21Cip1 promoter mutants showed that transcriptional repression of p21Cip1 by c-Myc was mediated through Miz-1- and Sp1-binding sites within the proximal region of the p21Cip1 promoter in normal IECs. These findings confirm that p21Cip1 is one of the direct mediators of induced c-Myc following increased polyamines and that p21Cip1 repression by c-Myc is implicated in stimulation of normal IEC proliferation.

Cell proliferation control by Notch signaling in Drosophila development

Development ◽  
1998 ◽  
Vol 125 (11) ◽  
pp. 2031-2040 ◽  
Author(s):  
M.J. Go ◽  
D.S. Eastman ◽  
S. Artavanis-Tsakonas
Keyword(s):  
Cell Proliferation ◽  
Notch Signaling ◽  
Imaginal Disc ◽  
Synergistic Effects ◽  
Wing Disc ◽  
Notch Receptor ◽  
Simple Consequence ◽  
Undifferentiated Cells ◽  
Cell Proliferation Control ◽  
Stimulation Of

The Notch receptor mediates cell interactions controlling the developmental fate of a broad spectrum of undifferentiated cells. By modulating Notch signaling in specific precursor cells during Drosophila imaginal disc development, we demonstrate that Notch activity can influence cell proliferation. The activation of the Notch receptor in the wing disc induces the expression of the wing margin patterning genes vestigial and wingless, and strong mitotic activity. However, the effect of Notch signaling on cell proliferation is not the simple consequence of the upregulation of either vestigial or wingless. Vestigial and Wingless, on the contrary, display synergistic effects with Notch signaling, resulting in the stimulation of cell proliferation in imaginal discs.

Stimulation of Early Childhood Intelligence through Interest Approach based on the Family Environment in the Pandemic Era

2021 ◽  
Vol 7 ◽  
pp. 00008
Author(s):  
Ismaniar Ismaniar ◽  
Setiyo Utoyo ◽  
Nur Hazizah
Keyword(s):  
Early Childhood ◽  
Home Environment ◽  
Family Environment ◽  
Literature Study ◽  
Approach To Learning ◽  
Study Approach ◽  
The Family ◽  
Learning Programs ◽  
Early Childhood Learning ◽  
Stimulation Of

The application of learning programs from home as an effort to reduce the transmission of the covid virus has brought about several phenomena in the field, including learning stimulation that is not carried out optimally, parents feel very bothered, and children are depressed and so on. All of this happened allegedly because some parents did not understand the approach to learning in early childhood. This research uses a literature study approach by utilizing various sources, both printed and online. From the results and discussion, it can be concluded that; 1. To optimize the results of intelligence stimulation/learning done at home, parents must understand the characteristics of early childhood learning in general, including children who like to play, each child is unique, and children like to imitate people in their environment. 2. Learning will take place well if parents understand and adapt the interests or tendencies of each child. 3. Every available space/spot in the home environment can be a fun place for intelligence stimulation for children if parents have sufficient understanding of the characteristics of an early child and the different interests of each child.

Cell type-specific requirement of the MAPK pathway for the growth factor action of gastrin

1999 ◽  
Vol 276 (6) ◽  
pp. G1363-G1372 ◽  
Author(s):  
Vinzenz M. Stepan ◽  
Chris J. Dickinson ◽  
John del Valle ◽  
Masashi Matsushima ◽  
Andrea Todisco
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Growth Factor ◽  
Protein Kinase ◽  
Mapk Pathway ◽  
Cell Type ◽  
Ar42j Cells ◽  
Cell Type Specific ◽  
Ar42j Cell ◽  
Stimulation Of

Gastrin (G17) has a CCKBreceptor-mediated growth-promoting effect on the AR42J rat acinar cell line that is linked to induction of both mitogen-activated protein kinase (MAPK) and c- fos gene expression. We investigated the mechanisms that regulate the growth factor action of G17 on the rat pituitary adenoma cell line GH3. Both AR42J and GH3cells displayed equal levels of CCKBreceptor expression and similar binding kinetics of125I-labeled G17. G17 stimulation of cell proliferation was identical in both cell lines. G17 stimulation of GH3cell proliferation was completely blocked by the CCKBreceptor antagonist D2 but not by the MEK inhibitor PD-98059 or the protein kinase C inhibitor GF-109203X, which completely inhibited G17 induction of AR42J cell proliferation. G17 induced a c- fos SRE-luciferase reporter gene plasmid more than fourfold in the AR42J cells, whereas it had no effect in the GH3cells. In contrast to what we observed in the AR42J cells, G17 failed to stimulate MAPK activation and Shc tyrosyl phosphorylation and association with the adapter protein Grb2. Epidermal growth factor induced the MAPK pathway in the GH3cells, demonstrating the integrity of this signaling system. G17 induced Ca2+mobilization in both the GH3and AR42J cells. The calmodulin inhibitor N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide inhibited AR42J cell proliferation by 20%, whereas it completely blocked G17 induction of GH3cell growth. The Ca2+ionophore ionomycin stimulated GH3cell proliferation to a level similar to that observed in response to G17, but it had no effect on AR42J cell proliferation. Thus there are cell type specific differences in the requirement of the MAPK pathway for the growth factor action of G17. Whereas in the AR42J cells G17 stimulates cell growth through activation of MAPK and c- fos gene expression, in the GH3cells, G17 fails to activate MAPK, and it induces cell proliferation through Ca2+-dependent signaling pathways. Furthermore, induction of Ca2+mobilization in the AR42J cells appears not to be sufficient to sustain cell proliferation.

scholarly journals MG-63 Cell Proliferation with Static or Dynamic Compressive Stimulation on an Auxetic PLGA Scaffold

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Myeong Jin Kim ◽  
Hong Jin Choi ◽  
Jongman Cho ◽  
Jung Bok Lee ◽  
Hak-Joon Sung ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Poisson Ratio ◽  
Dynamic Compression ◽  
Control Group ◽  
Plga Scaffold ◽  
Static Compression ◽  
Static Group ◽  
Stimulation Group ◽  
Dynamic Stimulation ◽  
Stimulation Of

The effect of dynamic compressive stimulation on MG-63 cell proliferation on an auxetic PLGA scaffold was investigated. The estimated Poisson ratio of the prepared auxetic scaffold specimens was approximately (−)0.07, while the Poisson ratio estimated for conventional scaffold specimens was (+)0.12 under 10% strain compression on average. Three stimulus groups were examined: control (no stimulation), static compression, and dynamic compression. In preparation for proliferation testing, cells were seeded at 2.2 × 105 cells/80 μL on each scaffold specimen. The average proliferation rates of the static and dynamic groups were higher than those of the control group: 13.4% and 25.5% higher at culture day 1, 34.7% and 56.2% at culture day 3, and 17.5% and 43.0% at culture day 5, respectively. The static and dynamic group results at culture day 5 were significantly different (p<0.01). Moreover, proliferation rate of the dynamic stimulation group was 1.22 times higher than that of the static group (p<0.01). Conclusively, proliferation of osteoblast-like cells was enhanced through compressive stimulation, but the enhancement was maximal with dynamic compressive stimulation of auxetic scaffolds.

scholarly journals Nuclear diacylglycerol is increased during cell proliferation in vivo

1993 ◽  
Vol 290 (3) ◽  
pp. 633-636 ◽  
Author(s):  
H Banfić ◽  
M Žižak ◽  
N Divecha ◽  
R F Irvine
Keyword(s):  
Cell Proliferation ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Nuclear Membrane ◽  
Cellular Proliferation ◽  
Detectable Change ◽  
Renal Growth ◽  
Kinase C ◽  
Stimulation Of

Highly purified nuclei were prepared from livers and kidneys of rats undergoing compensatory hepatic or renal growth, the former being predominantly by cellular proliferation, and the latter mostly by cellular enlargement. In liver, an increase in nuclear diacylglycerol (DAG) concentration occurred between 16 and 30 h, peaking at around 20 h. At the peak of nuclear DAG production a specific translocation of protein kinase C to the nucleus could be detected; no such changes occurred in kidney. There was no detectable change in whole-cell DAG levels in liver, and the increase in DAG was only measurable in nuclei freed of their nuclear membrane. Overall, these results suggest that there is a stimulation of intranuclear DAG production, possibly through the activation of an inositide cycle [Divecha, Banfić and Irvine (1991) EMBO J. 10, 3207-3214] during cell proliferation in vivo.

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