scholarly journals Essential oils of the leaves of Syzygium cumini (L.) Skeels and fruit peels of Hymenaea courbaril (L.) var. courbaril as molluscides against Biomphalaria glabrata

2020 ◽  
Vol 9 (10) ◽  
pp. e1239108215
Author(s):  
Gustavo Oliveira Everton ◽  
Paulo Sérgio Santos Júnior ◽  
Everton Holanda Sales ◽  
Paulo Victor Serra Rosa ◽  
Andressa Almeida Santana Dias ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Essential Oils ◽  
Chemical Characterization ◽  
Biomphalaria Glabrata ◽  
Artemia Salina ◽  
Major Constituent ◽  
Syzygium Cumini ◽  
Toxicity Assay ◽  
Hymenaea Courbaril ◽  
Molluscicidal Activity

This study evaluated the molluscicidal activity of essential oils (EOs) of Syzygium cumini L. and Hymenaea courbaril L. Hydrodistillation was used to extract the EOs. The chemical characterization was performed by Gas Chromatography coupled to mass spectrometry. The bioassay of Artemia salina Leach was used for the toxicity test. The molluscicidal assay tested concentrations of 10-80 mg L-1 of the EOs against Biomphalaria glabrata. The major constituent of The EO of S. cumini was isokaryophyllene and H. courbaril the Germacreno-D. The toxicity assay classified The EOs as nontoxic with LC50 412.10 mg L-1 for S. cumini and LC50 354.80 mg L-1 for H. courbaril. The molluscicidal assay purchased LC50 44.76/ LC90 77.20 for S. cumini and LC50 37.34 mg L-1/ LC90 73.24 mg L-1 for H. courbaril. The results showed that both EOs can be used as natural molluscicides in combating the snail Biomphalaria glabrata.

scholarly journals Chemical characterization, toxicity, antioxidant and antimicrobial activity of the essential oils of Hymenaea courbaril L. and Syzygium cumini (L.) Skeels

2021 ◽  
Vol 43 ◽  
pp. e11
Author(s):  
Gustavo Oliveira Everton ◽  
Ana Patrícia Matos Pereira ◽  
Paulo Victor Serra Rosa ◽  
Nilton Silva Costa Mafra ◽  
Paulo Sérgio Santos Júnior ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oils ◽  
Chemical Characterization ◽  
Artemia Salina ◽  
Major Constituent ◽  
Syzygium Cumini ◽  
Hymenaea Courbaril ◽  
E Coli ◽  
Low Toxicity ◽  
Antioxidant And Antimicrobial Activity

This study evaluated the antimicrobial, antioxidant and toxicity activity of essential oils (EOs) of Hymenaea courbaril L. var. courbaril bark and Syzygium cumini (L.) Skeels leaves. The EOs were extracted by hydrodistillation and chemically characterized by gas chromatography coupled to mass spectrometry (GC/MS). The ABTS and DPPH assay were used to evaluate antioxidant activity. For the toxicity assay, lethality was evaluated against Artemia salina Leach. For the antimicrobial assay, the method of Disc Diffusion and Dilution in Broth was applied to obtain the minimum inhibitory concentration and minimum bactericidal. The major constituent of the EO of H. courbaril was β-ocimene (23.33%) and the EO of S. cumini was isocaryophyllene (18.01%). Both OE showed relevant antioxidant activity and low toxicity against Artemia salina. The EOs showed bactericidal activity against E. coli, S. aureus, P. aeruginosa, Salmonella sp., B. cereus and P. mirabilis. The results obtained are encouraged by the potential use of the OE's studied in the control and fight of pathogenic microorganisms.

scholarly journals Suppression of Linalool Acetate Production in Lavandula x intermedia

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Amy Desautels ◽  
Kamal Biswas ◽  
Alexander Lane ◽  
Astrid Boeckelmann ◽  
Soheil S. Mahmoud
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Essential Oil ◽  
Essential Oils ◽  
Gas Chromatography Mass Spectrometry ◽  
Major Constituent ◽  
Improved Method ◽  
Acetate Production ◽  
Essential Oil Production ◽  
Sensitive Method

Linalool acetate, one of the major constituent of several essential oils, is heat-labile and decomposes upon exposure to the high injector temperature during gas chromatography. Here we report the development of an improved method for detection of this compound by gas chromatography mass spectrometry (GCMS) using cold on-column injection of the sample. By using this sensitive method, it has been demonstrated that a lavandin (L.x intermedia) mutant accumulates trace quantities of linalool acetate and camphor and higher amounts of cineole and borneol compared to its parent. This plant, which very likely carries a point mutation in one or more of the genes involved in essential oil production, provides a unique tool for investigating regulation of essential oil biogenesis in plants.

scholarly journals Molluscicidal activity of 2-hydroxy-[1,4]naphthoquinone and derivatives

2008 ◽  
Vol 80 (2) ◽  
pp. 329-334 ◽  
Author(s):  
Celso A. Camara ◽  
Tania M.S. Silva ◽  
Thiago G. da-Silva ◽  
Rodrigo M. Martins ◽  
Ticiano P. Barbosa ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
General Rule ◽  
Biomphalaria Glabrata ◽  
Artemia Salina ◽  
World Health ◽  
Significant Toxicity ◽  
The World ◽  
Health Organization ◽  
Polar Substituents ◽  
Molluscicidal Activity

The toxic profile of lawsone (2-hydroxy-[1,4]naphthoquinone) and a series of [1,4]naphthoquinone derivatives was evaluated against the brine shrimp Artemia salina and against the mollusk Biomphalaria glabrata, the main transmitting vector of schistosomiasis in Brazil. Of the seventeen compounds tested nine fell below the threshold of 100 µg/mL set for potential molluscicidal activity by the World Health Organization. As a general rule derivatives with non-polar substituents presented the highest molluscicidal activities. These substances showed significant toxicity in A. salina lethality bioassay.

scholarly journals Biological screening of extracts from leaf and stem bark of Croton floribundus Spreng. (Euphorbiaceae)

2018 ◽  
Vol 78 (4) ◽  
pp. 601-608 ◽  
Author(s):  
E. F. Barth ◽  
L. S. Pinto ◽  
P. Dileli ◽  
D. C. Biavatti ◽  
Y. L. Silva ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Biomphalaria Glabrata ◽  
Stem Bark ◽  
Artemia Salina ◽  
Preliminary Evaluation ◽  
Biological Screening ◽  
Significant Toxicity ◽  
Dpph Method ◽  
Acetylcholinesterase Enzyme ◽  
Molluscicidal Activity

Abstract This work describes the preliminary evaluation of cytotoxic, antimicrobial, molluscicidal, antioxidant and anticholinesterase activities from leaf (LECF) and stem bark alcoholic extracts (BECF) of the species Croton floribundus Spreng. (Euphorbiaceae), popularly known as capixingui or tapixingui. BECF presented significant toxicity (LC50 = 89.6 μg/ml) in the Artemia salina Leach, 1819 (Crustacea: Branchiopoda) bioassay, whereas LECF did not show activity (LC50 > 1000 μg/ml). From DPPH method, the values of IC50 for the LECF and BECF were 61.2 μg/ml and 62.2 μg/ml, respectively, showing that C. floribundus has an expressive antioxidant activity. Antimicrobial susceptibility was evaluated by microdilution technique and only BECF was active against Staphylococcus aureus (MIC = 39.6 μg/ml). The extracts did not present molluscicidal activity against snail Biomphalaria glabrata Say, 1818 (Gastropoda: Planorbidae). Both extracts revealed the presence of several components with an inhibiting capacity of acetylcholinesterase enzyme on the bioautographic assay. C. floribundus showed to be a promising species considering that it exhibited good biological activity in the most assays performed.

scholarly journals Chemical profile, bactericidal in vitro potential and toxicity against Artemia salina Leach of essential oils obtained from natural condiments

2021 ◽  
Vol 10 (2) ◽  
pp. e58310212898
Author(s):  
Thércia Gabrielle Teixeira Martins ◽  
Paulo Victor Serra Rosa ◽  
Ari Pereira de Araújo Neto ◽  
Ana Maria Almeida Silva Carvalho ◽  
Leila da Silva Silveira ◽  
...  
Keyword(s):  
Essential Oils ◽  
Low Cost ◽  
Artemia Salina ◽  
Market Potential ◽  
Dilution Method ◽  
Chemical Profile ◽  
Chemical Additives ◽  
Toxicity Assay ◽  
Plant Materials

Interest in essential oils and their food applications has grown due to the negative reaction of consumers to synthetic chemical additives intentionally added in industrialized products in order to increase their shelf life. In this context, the present study aimed to evaluate the chemical profile, toxicity to Artemia salina Leach and antibacterial activity in vitro of essential oils obtained from natural condiments on bacteria of clinical and food importance. Plant material was obtained in the municipality of São Luís-MA. The essential oils were extracted by hydrodistillation at 100 °C/3 h. Folin Ciocalteau methodology was performed for the determination of total phenolics. The toxicity assay was performed using the artemia salina Leach lethality bioassay. Antimicrobial activity followed the methodology described by the Clinical and Laboratory Standards Institute using the Broth Disc Diffusion and Dilution Method for the action of essential oils against the bacteria Escherichia coli, Salmonella sp., Staphylococcus aureus and Bacillus cereus. The presence of bioactive classes in the plant materials used in this research was identified and the essential oils obtained were classified as nontoxic in the toxicity assay, presenting low lethality to the micro crustacean Artemia salina Leach. In the bactericidal activity assay, the essential oils of O. vulgare, T. vulgaris, C. zeylanicum showed significant results, classified as efficient against the microorganisms tested. Finally, the use of essential oils classified as active and nontoxic is highlighted in this study as alternatives in the control and combat of pathogenic microorganisms presenting a proposal of natural product with low cost of obtaining and high market potential.

scholarly journals Antimicrobial activity of the essential oil from Bixa orellana L.

2020 ◽  
Vol 9 (10) ◽  
pp. e8129108447
Author(s):  
Yuri Nascimento Fróes ◽  
Ana Patrícia Matos Pereira ◽  
Paulo Victor Serra Rosa ◽  
Laiane Araujo da Silva Souto ◽  
Mariana Oliveira Arruda ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Total Phenolics ◽  
Chemical Characterization ◽  
Artemia Salina ◽  
Bixa Orellana ◽  
Toxicity Assay ◽  
Natural Substances ◽  
Application Potential ◽  
And Staphylococcus Aureus

The application of natural substances with bactericidal action is the target of research aimed at a more sustainable production, implying in various sources of substances for new therapeutic formulations. This study presents the chemical characterization, toxicity and antimicrobial activity of essential oil (EO) of Bixa orellana Labill leaves. EO was extracted by hydrodistillation at 100°C for 3h. Physicochemical parameters were determined and chemical characterization used Gas chromatography coupled with Mass Spectrometry (GC/MS). The toxicity assay was performed through the bioassay with Artemia salina Leach. The methods of Disc Diffusion and Dilution in Broth in front of the bacteria Escherichia coli and Staphylococcus aureus were used to evaluate the antimicrobial activity. LC50 in the toxicity assay was verified in 355 mg L-1 and was classified as nontoxic. The OE presented antimicrobial activity against the microorganisms tested and totaled 320 mg EAT g-1 of total phenolics. The results obtained highlight the importance of pointing out this product as a therapeutic alternative, encouraging its application potential.

scholarly journals Larvicidal activity, molluscicide and toxicity of the essential oil of Citrus limon peels against, respectively, Aedes aegypti, Biomphalaria glabrata and Artemia salina

2019 ◽  
Vol 44 (4) ◽  
pp. 85 ◽  
Author(s):  
Paulo Roberto Barros Gomes ◽  
Marlucy Bezerra Oliveira ◽  
Dionney Andrade De Sousa ◽  
Jeremias Caetano Da Silva ◽  
Romer Pessôa Fernandes ◽  
...  
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Biological Activities ◽  
Biomphalaria Glabrata ◽  
Artemia Salina ◽  
World Health ◽  
Lethal Concentration ◽  
Major Constituent ◽  
Citrus Limon

In this present work, we tested the larvicidal activity, molluscicide and toxicity of the oil extracted from Citrus limon peels, respectively against third stage larvae of Aedes aegypti, snail Biomphalaria glabrata, and Artemia salina. For this, we extract the essential oil by hydrodistillation. Then, we identified and quantified the components by gas chromatography coupled to mass spectrometry (GC-MS). We tested the larvicidal and molluscicidal activity, respectively, using the method adopted by the Brazilian Ministry of Health and the World Health Organization. We calculated the lethal concentration (LC50) from the Probit method for the three biological activities with 95 %. The results of the chromatographic analysis showed that the oil has 58.81% of Limonene (major constituent) and 0.11% α-Mulene (minority component). The essential oil presented lethal concentration (LC50) for larvicidal activity, molluscicide and toxicity, respectively at 15.48, 13.05 and 743.35 mg∙L-1. Therefore, the essential oil is active against larvae of A. aegypti and snail B. glabrata and non-toxic against larvae of A. salina.

Chemical characterization of Goniothalamus macrophyllus and Goniothalamus malayanus leaves’ essential oils

2020 ◽  
Vol 75 (11-12) ◽  
pp. 485-488
Author(s):  
Natasa Mohd Shakri ◽  
Wan Mohd Nuzul Hakimi Wan Salleh ◽  
Shamsul Khamis ◽  
Nor Azah Mohamad Ali
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Essential Oils ◽  
Chemical Characterization ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Compositions ◽  
Chromatography Mass Spectrometry ◽  
Germacrene D

AbstractThis study was aimed to investigate the chemical compositions of the essential oils from Goniothalamus macrophyllus and Goniothalamus malayanus growing in Malaysia. The essential oils were obtained by hydrodistillation and fully characterized by gas chromatography (GC–FID) and gas chromatography–mass spectrometry (GC–MS). Analyses of the essential oils from G. macrophyllus and G. malayanus resulted in 93.6 and 95.4% of the total oils, respectively. The major components of G. macrophyllus oil were germacrene D (25.1%), bicyclogermacrene (11.6%), α-copaene (6.9%) and δ-cadinene (6.4%), whereas in G. malayanus oil bicyclogermacrene (43.9%), germacrene D (21.1%) and β-elemene (8.4%) were the most abundant components.

scholarly journals Essential oil from two populations of Echinodorus grandiflorus (Cham. & Schltdl.) Micheli (Chapéu de couro)

2006 ◽  
Vol 78 (4) ◽  
pp. 623-628 ◽  
Author(s):  
Daniel S. Pimenta ◽  
Maria Raquel Figueiredo ◽  
Maria Auxiliadora C. Kaplan
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Essential Oil ◽  
Essential Oils ◽  
Gas Chromatography Mass Spectrometry ◽  
Major Constituent ◽  
Chromatography Mass Spectrometry ◽  
Different Populations ◽  
Two Populations

Analysis by Gas Chromatography and Gas Chromatography/Mass Spectrometry of the essential oils obtained from leaves of Echinodorus grandiflorus ("Chapéu de couro") from two different populations (Big Leaves and Small Leaves), collected monthly between September 1998 and December 1999 revealed 17 components. Phytol was the major constituent for both populations. The main sesquiterpene representatives are (E)-caryophyllene, alpha-humulene and (E)-nerolidol.

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