scholarly journals PENGARUH KONSENTRASI Carboxymethyl Cellulose (CMC) TERHADAP SIFAT FISIKOKIMIA DAN ORGANOLEPTIK SELAI KOPI DENGAN CARRIER LABU KUNING (Curcubita moschata Duchesne)

2021 ◽  
Vol 20 (2) ◽  
pp. 169-174
Author(s):  
Alvina Handoyo ◽  
◽  
Thomas Indarto Putut Suseno ◽  
Keyword(s):  
Carboxymethyl Cellulose ◽  
Ph Optimum

Selai merupakan produk pangan berbahan dasar buah yang ditambahkan air dan gula dan diproses hingga diperoleh konsistensi sesuai. Kopi adalah bahan yang dapat digunakan untuk membuat selai. Produktivitas kopi di Indonesia tinggi namun konsumsinya masih sangat rendah. Perlu ditambahkan carrier dan pengental agar diperoleh karakteristik fisik selai yang diinginkan. Pada penelitian ini digunakan Labu kuning (Curcubita moschata Duchesne) sebagai carrier dan Carboxymethyl Cellulose (CMC) sebagai pengental. CMC memiliki rentang pH yang cukup luas, yaitu 3-11 dengan pH optimum 5-10 sehingga dapat digunakan pada selai kopi yang memiliki pH 5-6. Tujuan penelitian ini adalah mengetahui pengaruh konsentrasi CMC terhadap sifat fisikokimia dan organoleptik selai kopi. Rancangan penelitian yang digunakan adalah Rancangan Acak Kelompok (RAK) dengan satu faktor, yaitu konsentrasi CMC, yaitu 0,60% (P1); 0,90% (P2); 1,20% (P3); 1,50% (P4); 1,80% (P5); 2,10% (P6) dari berat bubur buah. Pengulangan dilakukan sebanyak empat kali. Perlakuan terbaik diperoleh dengan menghitung luas segitiga dari parameter Aw, rasa, aroma, mouthfeel. Parameter lain yang diuji yaitu daya oles, viskositas, sineresis, dan warna. Berdasarkan hasil penelitian diketahui ada pengaruh konsentrasi CMC terhadap sifat fisikokimia dan organoleptik selai kopi dengan carrier labu kuning. Peningkatan konsentrasi CMC menyebabkan penurunan Aw, daya oles dan sineresis serta peningkatan viskositas. Rata-rata kesukaan konsumen terhadap rasa 3,82-4,11, mouthfeel 4,28-4,82, aroma 4,24-5,14 dengan skala skor 1-7. Perlakuan terbaik dengan luas segitiga 68,0353, Aw 0,930, daya oles 24,91 cm2, viskositas 9442,25 cP, sineresis 5,06-12,53%, tingkat kesukaan panelis terhadap rasa agak tidak suka, mouthfeel netral, aroma agak suka adalah selai dengan konsentrasi CMC 0,60%.

A novel ribonuclease from fresh fruiting bodies of the portabella mushroom Agaricus bisporus

2006 ◽  
Vol 84 (2) ◽  
pp. 178-183 ◽  
Author(s):  
H X Wang ◽  
T B Ng
Keyword(s):  
Carboxymethyl Cellulose ◽  
Agaricus Bisporus ◽  
Deae Cellulose ◽  
Fruiting Bodies ◽  
Terminal Sequence ◽  
Temperature Optimum ◽  
Ph Optimum

A 14 kDa ribonuclease with a novel N-terminal sequence was isolated from fresh fruiting bodies of the portabella mushroom. It was adsorbed on DEAE-cellulose and carboxymethyl-cellulose, and demonstrated the highest ribonucleolytic potency toward poly (A), 60% as much activity toward poly (C), 40% as much activity toward poly (U), and the least activity (7% as much) toward poly (G). It exhibited a pH optimum at pH 4.5 and a temperature optimum at 60 °C. Its activity at 100 °C was higher than that at 20 °C.Key words: ribonuclease, portabella mushroom, isolation.

The Uptake of Adenine by Isolated Human Platelet Membranes

1974 ◽  
Vol 32 (02/03) ◽  
pp. 457-464
Author(s):  
Paul C. French ◽  
Jan J. Sixma ◽  
Holm Holmsen
Keyword(s):  
Human Platelet ◽  
Facilitated Diffusion ◽  
Adenine Phosphoribosyltransferase ◽  
Ph Optimum ◽  
Intact Cells ◽  
Platelet Membranes ◽  
Group Translocation ◽  
Triton X

SummaryAdenine uptake into isolated platelet membranes had about the same Km (151 ± 21 • 9 nM) as uptake into intact cells (159 ± 21 nM) and was also competitively inhibited by papaverine and hypoxanthine. No uptake occurred at 0° and accumulated adenine was converted to AMP. AMP was not firmly bound to protein as judged by chromatography of triton X-100 solubilized membranes on Sephadex G25. The pH optimum for adenine uptake was at pH 5-5. Exogenous 5-phosphoribosyl-l-pyrophos- phate strongly stimulated uptake. These data may be explained by uptake of adenine by facilitated diffusion followed by conversion to AMP by adenine phosphoribosyltransferase but group translocation cannot be entirely excluded.

Isolation and Characterization of Plasminogen Activator from Pig Leucocytes

1974 ◽  
Vol 31 (01) ◽  
pp. 072-085 ◽  
Author(s):  
M Kopitar ◽  
M Stegnar ◽  
B Accetto ◽  
D Lebez
Keyword(s):  
Molecular Weight ◽  
Plasminogen Activator ◽  
Gel Electrophoresis ◽  
Gel Filtration ◽  
Ph Optimum ◽  
Isolation And Characterization ◽  
Ph Range ◽  
Inhibition Studies ◽  
Final Purification

SummaryPlasminogen activator was isolated from disrupted pig leucocytes by the aid of DEAE chromatography, gel filtration on Sephadex G-100 and final purification on CM cellulose, or by preparative gel electrophoresis.Isolated plasminogen activator corresponds No. 3 band of the starting sample of leucocyte cells (that is composed from 10 gel electrophoretic bands).pH optimum was found to be in pH range 8.0–8.5 and the highest pH stability is between pH range 5.0–8.0.Inhibition studies of isolated plasminogen activator were performed with EACA, AMCHA, PAMBA and Trasylol, using Anson and Astrup method. By Astrup method 100% inhibition was found with EACA and Trasylol and 30% with AMCHA. PAMBA gave 60% inhibition already at concentration 10–3 M/ml. Molecular weight of plasminogen activator was determined by gel filtration on Sephadex G-100. The value obtained from 4 different samples was found to be 28000–30500.

Complex formation of modified chitosan and carboxymethyl cellulose and its effect on paper properties

TAPPI Journal ◽  
2009 ◽  
Vol 8 (6) ◽  
pp. 29-35 ◽  
Author(s):  
PEDRAM FATEHI ◽  
LIYING QIAN ◽  
RATTANA KITITERAKUN ◽  
THIRASAK RIRKSOMBOON ◽  
HUINING XIAO
Keyword(s):  
Carboxymethyl Cellulose ◽  
Polymer System ◽  
Layer By Layer ◽  
Paper Strength ◽  
Anionic Polymer ◽  
Paper Properties ◽  
Modified Chitosan ◽  
Layer By Layer Assembly ◽  
Polyelectrolyte Titration ◽  
Layer Assembly

The application of an oppositely charged dual polymer system is a promising approach to enhance paper strength. In this work, modified chitosan (MCN), a cationic polymer, and carboxymethyl cellulose (CMC), an anionic polymer, were used sequentially to improve paper strength. The adsorption of MCN on cellulose fibers was analyzed via polyelectrolyte titration. The formation of MCN/CMC complex in water and the deposition of this complex on silicon wafers were investigated by means of atomic force microscope and quasi-elastic light scattering techniques. The results showed that paper strength was enhanced slightly with a layer-by-layer assembly of the polymers. However, if the washing stage, which was required for layer-by-layer assembly, was eliminated, the MCN/CMC complex was deposited on fibers more efficiently, and the paper strength was improved more significantly. The significant improvement was attributed to the extra development of fiber bonding, confirmed further by scanning electron microscope observation of the bonding area of fibers treated with or without washing. However, the brightness of papers was somewhat decreased by the deposition of the complex on fibers. Higher paper strength also was achieved using rapid drying rather than air drying.

Efektivitas Biokoagulan Cangkang Telur Ayam Ras dan Kulit Pisang Kepok (Musa Balbisiana ABB) dalam Menurunkan Turbiditas, TDS, dan TSS dari Limbah Cair Industri Farmasi

al-Kimiya ◽  
2020 ◽  
Vol 7 (1) ◽  
pp. 47-54
Author(s):  
Hesty Nuur Hanifah ◽  
Ginayanti Hadisoebroto ◽  
Turyati Turyati ◽  
Ineu Sintia Anggraeni
Keyword(s):  
Total Suspended Solid ◽  
Suspended Solid ◽  
Ph Optimum ◽  
Musa Balbisiana

Koagulasi merupakan tahap awal dalam proses pengolahan limbah cair. Salah satu industri yang berpotensi untuk menimbulkan pencemaran air bila limbah cairnya tidak dikelola dengan baik adalah industri farmasi. Cangkang telur ayam ras dan kulit pisang kepok merupakan limbah padat yang belum termanfaatkan, padahal kedua bahan tersebut mengandung zat-zat yang bisa membantu dalam proses koagulasi. Oleh karena itu, peneliti tertarik untuk mengembangkan biokoagulan dari cangkang telur ayam ras dan kulit pisang kepok. Penelitian ini bertujuan untuk mengetahui efektivitas dari cangkang telur ayam ras dan kulit pisang kepok sebagai biokoagulan dalam menurunkan nilai turbiditas, TDS (Total Disolved Solid) dan TSS (Total Suspended Solid) dari limbah cair industri farmasi. Alat yang digunakan dalam penelitian ini adalah jartest. Sampel air limbah yang digunakan dalam percobaan ini yaitu sampel air limbah industri farmasi dari PT Sinkona Indonesia Lestari. Berdasarkan hasil penelitian  menunjukkan bahwa biokoagulan cangkang telur ayam mempunyai dosis optimum yaitu 50 g/500 mL dan pada pH optimum 8 terjadi penurunan turbiditas sebesar 81,18%, TDS sebesar 24,3% dan TSS sebesar 82,05%. Sedangkan  biokoagulan kulit pisang kepok mempunyai dosis optimum 5 g/500 ml dan pada pH optimum 2 terjadi penurunan  turbiditas sebesar   94,9%, TDS 51,3% dan TSS  83,2%. Dari data tersebut bisa disimpulkan bahwa cangkang telur ayam ras dan kulit pisang kepok bisa dimanfaatkan sebagai biokoagulan untuk pengolahan limbah cair dari industri farmasi.

Effects of Trehalose and Sodium Carboxymethyl Cellulose on Prevention of Organ Adhesion after Laparotomy: A Preliminary Study

2009 ◽  
Vol 40 (2) ◽  
pp. 19-26 ◽  
Author(s):  
Soojung LEE ◽  
Yasutsugu MIWA ◽  
Ryohei NISHIMURA ◽  
Ung-il CHUNG ◽  
Shigeki SUZUKI ◽  
...  
Keyword(s):  
Carboxymethyl Cellulose ◽  
Sodium Carboxymethyl Cellulose ◽  
Preliminary Study

FERMENTATIVE HYDROLYTIC PREPARATION METHODS OF CEREAL WORT FOR ALCOHOL FERMENTATION

1970 ◽  
pp. 52-56
Author(s):  
Е. M. Serba ◽  
М. B. Overchenko ◽  
L. V. Rimareva ◽  
N. I. Ignatova ◽  
А. E. Orekhova ◽  
...  
Keyword(s):  
Alcoholic Fermentation ◽  
Raw Materials ◽  
Alcohol Concentration ◽  
Activity Level ◽  
Optimal Dosage ◽  
Main Role ◽  
Alcohol Fermentation ◽  
Ph Optimum ◽  
Amylolytic Enzyme ◽  
Enzyme Preparations

In the production of alcohol in the preparation of grain raw materials for fermentation, the main role is given to enzyme preparations of amylolytic action, which are key enzymes that catalyze the hydrolysis of starch. Amylolytic enzyme preparations with a different composition of enzymes and their level of activity, a mechanism of biocatalytic effect on starch, and a range of thermal and pH optimum are widely represented on the Russian market. The development of optimal conditions for the preparation of grain wort, the rational selection and dosage of concentrated enzyme preparations, the properties of which correspond to the parameters of the technological process, will ensure the effective preparation of starch for fermentation, and increase the profitability of alcohol production. The aim of this work was to study the influence of enzyme preparations of amylolytic action and the conditions of their use on the efficiency of the process of alcoholic fermentation and the yield of the final product, ethanol. The effect of various dosages of enzyme preparations of glucoamylase action, with a different ratio of the main enzyme glucoamylase and minor enzyme α-amylase, as well as methods for preparing wheat wort on the process of alcoholic fermentation, was studied. It was found that the enzyme preparation, the source of glucoamylase, in which α-amylase was present in a ratio of 15: 1 (in terms of activity level), turned out to be more effective in fermenting prepared wheat wort: its optimal dosage was 8 units. GLS / g starch. The presence of a sufficient amount of α-amylase in this preparation compensated for the dosage of thermostable α-amylase. The alcohol concentration in the mash was 10.2% vol., The alcohol yield was 67.9 cm3 / 100 g of starch. When glucoamylase with a lower ratio of the main and minor enzyme (75: 1) was used at the saccharification stage, an increase in the wort fermentation depth was observed with an increase in the concentration of glucoamylase to 9-10 units of GLS / g and α-amylase to 0.5 units. AC / g. It was also found that an increase in the duration of enzymatic-hydrolytic preparation of the wort had a positive effect on the fermentation process, the alcohol concentration in the mash increased to 10.2 vol.%. It was shown that the introduction of proteases into the wort helps to reduce the viscosity of grain wort, enriching it with assimilable yeast amino acids, which leads to an increase in the yield of alcohol. It has been confirmed that the synergy of the action of enzymes of amylolytic and proteolytic effects on polymers of grain raw materials allows to increase the efficiency of their conversion to ethanol. The conditions of enzymatic-hydrolytic processing of grain raw materials for fermentation are developed. The use of the digestion stage did not significantly affect the fermentation results of wheat wort.

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