scholarly journals ACUTE TOXICITY, PHYTOCHEMICAL SCREENING, AND ANTIMICROBIAL INVESTIGATION OF AQUEOUS EXTRACT OF SODOM APPLE TREE (Calotropis procera Ait.F) LEAF OBTAINED WITHIN ANCHOR UNIVERSITY, LAGOS

2021 ◽  
Vol 3 (2) ◽  
pp. 121
Author(s):  
Olatunde A Oseni ◽  
Hilda A Emmanuel-Akerele ◽  
Wuraola A Adebayo
Keyword(s):  
Acute Toxicity ◽  
Aqueous Extract ◽  
Apple Tree ◽  
Calotropis Procera ◽  
Phytochemical Screening ◽  
Sodom Apple

scholarly journals Antidiarrhoeal activity of aqueous leaf extract of Olea europaea var. sylvestris in albino Wistar rats

2018 ◽  
Vol 91 (2) ◽  
Author(s):  
Mohamed Zaouani ◽  
Fatima Yahiaoui ◽  
Nazli Nacer Bey ◽  
Meriem Hind Ben-Mahdi
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Aqueous Extract ◽  
Olea Europaea ◽  
Castor Oil ◽  
Wistar Rats ◽  
Toxicity Study ◽  
Motility Assay ◽  
Phytochemical Screening ◽  
Leaf Aqueous Extract

Olea europaea var. sylvestris, also named oleaster, is widely used by traditional medicine practitioners in Algeria to treat high blood pressure and diabetes. However, the antidiarrhoeal activity of this plant has not been scientifically evaluated. The main aim of the study deals with an investigation of three topics: the phytochemical screening, the acute toxicity, and antidiarrhoeal activity of the oleaster leaf aqueous extract. Acute oral toxicity study was carried out based on Organization for Economic Cooperation and Development 423 guideline. The extract was orally administered in wistar rats at a single dose of 2000 mg/kg body weight and the animals were observed for mortality, behavioral changes and other abnormal signs. Qualitative analysis of phytochemical constituents was carried out using standard methods developed by Harborne, Trease and Evans. Castor oil-induced diarrhoea tests and gastro intestinal motility assay were evaluated in rats to determine the antidiarrhoeal activity of the extract. In the acute toxicity study, the extract did not induce death or any sign of toxicity in treated rats. The preliminary phytochemical screening of the extract revealed the presence of saponins, flavonoids, and triterpenoids. The oleaster extract at oral doses of 100, 200 and 400 mg/kg body weight showed a significant (P<0.05) antidiarrhoeal activity compared to the control group treated with castor oil induced diarrhoea, enteropooling and gastrointestinal motility assay, after charcoal meal administration. The oleaster leaf aqueous extract has shown a gradual response with increasing dose. The present study indicates that the oleaster leaf aqueous extract is safe with antidiarrhoeal property.

Phytochemical screening, acute toxicity and analgesic properties of aqueous extract of flueggea virosa's root in rats

2013 ◽  
Vol 5 (1) ◽  
pp. 15 ◽  
Author(s):  
JOC Ezeonwumelu ◽  
EK Matuki ◽  
AM Ajayi ◽  
AG Okoruwa ◽  
JK Tanayen ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Aqueous Extract ◽  
Phytochemical Screening

scholarly journals Phytochemical Screening and Acute Toxicity of Aqueous Extract of Leaves of Conocarpus erectus Linnaeus in Swiss Albino Mice

2016 ◽  
Vol 88 (3) ◽  
pp. 1431-1437 ◽  
Author(s):  
DAYANE K.D. NASCIMENTO ◽  
IVONE A. DE SOUZA ◽  
ANTÔNIO F.M. DE OLIVEIRA ◽  
MARIANA O. BARBOSA ◽  
MARLLON A.N. SANTANA ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Aqueous Extract ◽  
Plant Material ◽  
Folk Medicine ◽  
Phytochemical Screening ◽  
Biological Productivity ◽  
Bioactive Substances ◽  
Swiss Albino Mice ◽  
Conocarpus Erectus ◽  
Albino Mice

ABSTRACT Mangroves represent areas of high biological productivity and it is a region rich in bioactive substances used in medicine production. Conocarpus erectus (Combretaceae) known as button mangrove is one of the species found in mangroves and it is used in folk medicine in the treatment of anemia, catarrh, conjunctivitis, diabetes, diarrhea, fever, gonorrhea, headache, hemorrhage, orchitis, rash, bumps and syphilis. The present study aimed to investigate the acute toxicity of aqueous extract of leaves of C. erectus in Swiss albino mice. The plant material was collected in Vila Velha mangroves, located in Itamaracá (PE). The material was subjected to a phytochemical screening where extractive protocols to identify majority molecules present in leaves were used. The evaluation of acute toxicity of aqueous extract of C. erectus followed the model of Acute Toxicity Class based on OECD 423 Guideline, 2001. The majority molecules were identified: flavonoids, tannins and saponins. The LD50 was estimated at 2,000 mg/kg bw. Therefore, the aqueous extract showed low acute toxicity classified in category 5.

scholarly journals Evaluation of antidiabetic activity of aqueous extract of Origanum floribundum Munby leaves in alloxan induced diabetic rats wistar

2021 ◽  
Vol 93 (2) ◽  
Author(s):  
M'hamed Nasri ◽  
Mohamed Zaouani ◽  
Noura Mimoune ◽  
Fatma Amira Hani ◽  
Mohamed Mahdid ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Aqueous Extract ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Albino Rats ◽  
Phytochemical Screening ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Standard Procedures ◽  
Wistar Albino Rats

The present study aimed to evaluate the phytochemicals and the antidiabetic properties of Origanum floribundum aqueous extract. Phytochemical screening was conducted by using various standard procedures. Acute toxicity of the extract was determined by OECD guidelines 423. The antidiabetic activity of the plant was determined by alloxan-induced diabetes in Wistar albino rats. Data obtained showed the detection of various secondary metabolites such as alkaloids, flavonoids, saponins, terpenoids in the extract. No mortality was observed during acute toxicity studies up to the dose of 2000 mg/kg. The aqueous extract presented the ability of reducing blood glucose levels. These findings indicated that Origanum floribundum aqueous extract had various phytopharmacological activities and thus it would be useful to isolate and characterize the compounds responsible for these bioactivities in the future.

Antihyperglycemic, Antihyperlipidemic and Antioxidant Effects of Cotula cinerea (Del) in Normal and Streptozotocin-Induced Diabetic Rats

2020 ◽  
Vol 20 (9) ◽  
pp. 1504-1513 ◽  
Author(s):  
Ayoub Amssayef ◽  
Mohamed Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Glucose Tolerance ◽  
Oral Administration ◽  
Beneficial Effect ◽  
Aqueous Extract ◽  
Aerial Part ◽  
Diabetic Rats ◽  
Histopathological Analysis ◽  
Phytochemical Screening ◽  
Cotula Cinerea

Aims: The current investigation aimed to assess the antioxidant, antidiabetic and antilipidemic effects of the aqueous extract of aerial part of Cotula cinerea (C. cinerea). Background: Cotula cinerea (Del). which belongs to the Asteraceae family is commonly used traditionally for the treatment of diabetes. Objective: The objective of the study was to study the effect of the aqueous C. cinerea extract on glucose and lipid metabolism in normal and streptozotocin-induced diabetic rats using a single and repeated oral administration. Methods: A preliminary phytochemical screening and the quantification of phenolic and flavonoid contents as well as the antioxidant activity using three methods (DPPH, FRAP and ABTS) were carried out. The effect of a single and repeated (15 days of treatment) oral administration of the aqueous extract of aerial part of Cotula cinerea (AEAPCC) at a dose of 20 mg/kg on glucose and lipid profile was examined in normal and streptozotocin-induced diabetic rats. Additionally, histopathological examination of the pancreas and liver was carried out according to the Hematoxylin-Eosin method. Results: AEAPCC (20 mg/kg) showed a significant blood glucose-lowering activity in both normal and diabetic rats after a single and repeated oral administration during 15 days. The aqueous extract was also able to decrease the plasma triglycerides levels in both normal and diabetic rats after 15 days of oral treatment at a dose of 20 mg/Kg while no effect was observed on plasma cholesterol levels. In addition, the results show that AEAPCC exhibits an in vitro antioxidant activity using different tests. Histopathological analysis of the pancreas and liver of AEAPCC-treated diabetic rats has revealed that AEAPCC had a beneficial effect on the architecture of these organs while no improvement of glucose tolerance was noticed using the glucose tolerance test. Furthermore, the results showed that the extract is rich in several phytochemical compounds and exhibited an important antioxidant activity. The phytochemical screening revealed that AEAPCC contains polyphenolic compounds, flavonoids, tannins, alkaloids, saponins, quinones, sterols, terpenoids, anthroquinones and reducing sugars. Whereas, it is free from glycosides. Conclusion: In conclusion, this study demonstrates that Cotula cinerea possesses a beneficial effect on diabetes. Further investigations are required to study the mechanism of action of the antidiabetic effect of this plant.

In-vitro and in-vivo evaluation of antidiabetic activity of Withania coagulans extract

2019 ◽  
Vol 09 ◽  
Author(s):  
Tejas Patel ◽  
B.N. Suhagia
Keyword(s):  
Blood Glucose ◽  
Acute Toxicity ◽  
Aqueous Extract ◽  
Pancreatic Beta Cell ◽  
Toxicity Study ◽  
Acute Toxicity Study ◽  
Withania Coagulans ◽  
Study Results

Background: Diabetes mellitus is major issue to public health as its prevalence is rising day by day. Synthetic agents available for the diabetic treatment are expensive or produce undesirable side effect on chronic use and some of them are not suitable during pregnancy. Herbal medicines accepted widely due to side effects and low cost. Objective: The aim of present study was to evaluate the activity of Withania coagulans extract using In-vitro and In-vivo model. Methods: Different three types of Withania coagulans extract were prepared using aqueous (W1), Alcohol (W2) and hydro-alcoholic (50:50) mixture (W3). In-vitro Anti-diabetic activity of the all three extracts evaluated using RINm5F Pancreatic beta cells.Further, n-vivo anti-diabetic evaluation performed by administering 50 mg/kg (p.o) aqueous extract for 7 days in Streptozotocin (STZ)-induced mice. Body weight of the animals was also determined to perform acute toxicity study. Results: The results of in –vitro cell based study indicated that among all three extract, aqueous extract (W1) of Withania coagulans showed potential increase in inulin release. The EC50 of the W1 (249.6 µg/L) which is compared with standard (Glibenclamide) EC50. From the results of In-vitro study, W1 subjected for acute toxicity study and the acute toxicity study results indicated LD50 of 50mg/kg. Diabetic rats treated with W1 extract at oral dose of 50 mg/kg for 7 days showed 34.17% reduction in blood glucose in comparison to untreated diabetic (STZ-induced) rats. Blood glucose levels of Standard treated (Glibenclamide) and control untreated. Conclusion: In conclusion, results of pancreatic beta cell based study showed increase in insulin release by administration of extract. Further aqueous extract (W1) was potentially reduced blood glucose level in STZ induced diabetic mice.

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