scholarly journals Comparison of modern quantitative methods for determining level of D-dimer based on immunoturbidimetry

2019 ◽  
Vol 3 (22) ◽  
pp. 56-57
Author(s):  
A. N. Mamaev ◽  
M. V. Pykhteeva ◽  
S. S. Terekhov ◽  
A. V. Kudinov ◽  
D. A. Trukhina ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Quantitative Methods ◽  
Research Study ◽  
Lower Limbs ◽  
Control Group ◽  
Blood Samples ◽  
Thrombotic Complications ◽  
D Dimer ◽  
Duration Of Gestation

The object of this paper is the comparative analysis of the results of determination of the D-dimer level with the new home-produced reagent on the basis of original monoclonal antibodies with the results of the widespread diagnostic analogues. The research study included blood samples of 80 patients. 22 of these patients (27.5 %) were diagnosed venous thrombosises of the lower limbs, 28 patients (35.0 %) did not have any thrombotic complications (control group). Besides, the research included 30 pregnant women (37.5 %) with different duration of gestation. Despite some distinctions when determining the D-dimer level, contemporary quantitative methods of the D-dimer level study demonstrate comparability of the research results which is documented by the high rates of correlation. The new diagnostic kit Tech-D-dimer-auto, based on the use of original monoclonal antibodies, demonstrates good comparability with analogues and is suitable for detection of thrombosis.

Changes in blood acid-base balance parameters and coagulation profile during diarrhea in calves

2013 ◽  
Vol 16 (3) ◽  
pp. 543-549 ◽  
Author(s):  
P. Sobiech ◽  
W. Rękawek ◽  
M. Ali ◽  
R. Targoński ◽  
K. Żarczyńska ◽  
...  
Keyword(s):  
Control Group ◽  
Thrombin Time ◽  
Acid Base Balance ◽  
Acid Base ◽  
Blood Samples ◽  
Coagulation Profile ◽  
At Iii ◽  
Base Balance ◽  
Diarrheic Calves ◽  
D Dimer

Abstract The purpose of this study was to investigate possible alterations in acid-base balance parameters and the coagulation profile in neonatal diarrheic calves. Twenty neonatal diarrheic and 20 clinically healthy neonatal calves aged between 1 week to 10 days were used. All blood samples were taken on the third day from the onset of diarrhea symptom. Venous blood samples were collected from each animal to determine platelet numbers, pH, pCO2, pO2, HCO3-, BE, O2SAT, ctCO2 and electrolytes (K+, Na+ and Cl-). Plasma samples were collected from each animal for the measurement of prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), the concentrations of fibrinogen, D-dimer and the activity of antithrombin III (AT III). Blood pH (7.19), BE (-10.6 mmol/l), HCO3 - (25.15 mmol/l), pO2 (3.33 kPa), O2SAT (24.12 %) were significantly lower and serum concentration of K+ (6.55 mmol/l) was significantly higher in diarrheic calves. These changes indicate the state of uncompensated metabolic acidosis with accompanying hyperkalemia. TT (32.05s) and APTT (39.9s) values were more prolonged in calves with diarrhea than in the control group. D-dimer (587.25 μg/l) concentrations were significantly increased while a visible drop in AT III (103.75%) activity and platelets counts (598 x109/l) were observed in diarrheic group of calves. The results suggest that a consumptive type of disseminated intravascular coagulation (DIC) developed in diarrheic calves.

Binding Properties of Monoclonal Antibodies against Human Fragment D-Dimer of Cross-Linked Fibrin to Human Plasma Clots in an In Vivo Model in Rabbits

1989 ◽  
Vol 61 (02) ◽  
pp. 307-313 ◽  
Author(s):  
P Holvoet ◽  
J M Stassen ◽  
Y Hashimoto ◽  
D Spriggs ◽  
P Devos ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Human Plasma ◽  
Jugular Vein ◽  
Degradation Products ◽  
Control Group ◽  
Human Fibrinogen ◽  
Fab Fragments ◽  
Vein Segment ◽  
D Dimer

SummaryTwo (MA-15C5 and MA-8D3) out of approximately 500 monoclonal antibodies, obtained by fusion of P3X63-Ag8-6.5.3 myeloma cells with spleen cells of mice immunized with purified fragment D-dimer from human fibrin, demonstrated a more than 1,000-fold higher affinity for fragment D-dimer than for native fibrinogen. MA-15C5 was directed against a neoantigenic determinant only expressed in fragment D-dimer. MA-8D3 reacted equally well with fragment D-dimer of crosslinked fibrin and with fragment D of non-crosslinked fibrin but not with fragment D of fibrinogen. Both monoclonal antibodies did not crossreact with rabbit fibrin and its degradation products.The binding of 125I-labeled Fab fragments to human plasma clots, introduced and aged for 1 hr in the jugular vein of heparinized rabbits was studied. Following injection of an equimolar mixture of Fab fragments derived from MA-15C5 and MA-8D3, the clot to blood ratios of radioactivity increased from 3.2 ± 1.2(mean ± SD) at 4 hr to 7.2 ± 1.4 at 17 hr. The binding of Fab fragments of MA-15C5 and MA-8D3 was independent of the age (1 to 72hrs) of the clot and of heparin anticoagulation and was only slightly decreased (by 20%) in the presence of circulating human fibrinogen (90 mg/kg body weight) and of human crosslinked fibrin degradation products at a plasma concentration of 10 pg/ml. The binding of Fab fragments of MA-15C5 and MA-8D3 to occlusive human plasma clots in the femoral artery of rabbits was comparable to that of the non-occlusive human plasma clots in the jugular vein. The Fab fragments of MA-15C5 and MA-8D3, labeled with 123I to a specific activity of 10 μCi/pg were injected intravenously (3 μg/kg) in 72 rabbits with a nonocclusive 0.2 ml human plasma clot in the jugular vein and in 7 control rabbits that underwent the surgical procedure without clot formation. Total body scans performed at hourly intervals revealed a higher relative increase in gamma counts over the thrombus region in the group with thrombus as compared to controls: at 6 hr 54 ± 18 vs 16 ± 13% (mean ± SEM, p <0.1) and at12 hrs 35 ± 11 vs –7 ± 12 (p <0.05). The vein segment to blood ratios of 123I at 24 hrs were 6.6 ± 2.4 in the group with clot and 1.5 to 0.7 in the control group (p <0.01). We conclude that these Fab fragments may have a sufficiently high fibrin-affinity to allow in vivo thrombus localization by external scanning.

scholarly journals Hypercoagulation and Hypermetabolism of Fibrinogen in Severely Burned Adults

2019 ◽  
Author(s):  
Wenjun Z Martini ◽  
John B Holcomb ◽  
Yong-Ming Yu ◽  
Steven E Wolf ◽  
Leopoldo C Cancio ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Vital Signs ◽  
Institutional Review Board ◽  
Control Group ◽  
Clot Strength ◽  
Blood Samples ◽  
Respiration Rates ◽  
Institutional Review ◽  
Production And Consumption ◽  
D Dimer

Abstract This study investigated changes in plasma fibrinogen metabolism and changes in coagulation in severely burned adults. Ten patients (27 ± 3 years; 91 ± 6 kg) with 51 ± 3% TBSA were consented and enrolled into an institutional review board–approved prospective study. On the study day, stable isotope infusion of 1-13C-phenylalanine and d5-phenylalanine was performed to quantify fibrinogen production and consumption. During the infusion, vital signs were recorded and blood samples were drawn every hour. Coagulation was measured by thromboelastograph (TEG). Ten normal healthy volunteers (37 ± 7 years; 74 ± 4 kg) were included as the control group. Burned adults had elevated heart rates (120 ± 2 vs 73 ± 5 [control] beats/minute), respiration rates (23 ± 2 vs 15 ± 1 breaths/minute), plasma glucose (127 ± 10 vs 89 ± 2 mg/dl), and fibrinogen levels (613 ± 35 vs 239 ± 17 mg/dl); and decreased albumin (1.3 ± 0.2 vs 3.7 ± 0.1 g/dl) and total protein (4.4 ± 0.2 vs 6.8 ± 0.1 g/dl, all P < .05). Fibrinogen breakdown was elevated in the burn group (2.3 ± 0.4 vs. 1.0 ± 0.3 µmol/kg/minute); and fibrinogen synthesis was further enhanced in the burn group (4.4 ± 0.7 vs 0.7 ± 0.2 µmol/kg/minute, both P < .05). Clotting speed (TEG-alpha) and clot strength (TEG-MA) were increased in the burn group (62 ± 4 vs 50 ± 4°, and 76 ± 2 vs 56 ± 2 mm, respectively, both P < .05). Fibrinolysis of TEG-LY60 was accelerated in the burn group (16 ± 6 vs 3 ± 1) and so was the increase in D-dimer level in the burn group (4.5 ± 0.4 vs 1.9 ± 0.3 mg/l, both P < .05). The hypercoagulable state postburn is in part a result of increased fibrinogen synthesis, over and above increased fibrinogen breakdown.

The value of combined elevation of D-dimer and neopterin as a predictive parameter for early stage acute mesenteric ischemia: An experimental study

Vascular ◽  
2016 ◽  
Vol 25 (2) ◽  
pp. 163-169 ◽  
Author(s):  
Ali Kagan Coskun ◽  
Zekai Halici ◽  
Akgun Oral ◽  
Yasin Bayir ◽  
Ferhat Deniz ◽  
...  
Keyword(s):  
Experimental Study ◽  
Superior Mesenteric Artery ◽  
Mesenteric Artery ◽  
Mesenteric Ischemia ◽  
Early Stage ◽  
Acute Mesenteric Ischemia ◽  
Artery Occlusion ◽  
Control Group ◽  
Blood Samples ◽  
D Dimer

Background The diagnosis of acute mesenteric ischemia is variable. Early diagnosis is important for reducing the mortality and morbidity rates. Aim This experimental study aims to investigate the diagnostic utility of D-dimer and neopterin as a marker for the early stage of acute mesenteric ischemia caused by occlusion of superior mesenteric artery. Methods The levels of D-dimer and neopterin were measured using an animal acute mesenteric ischemia model in 21 male rabbits. Superior mesenteric artery occlusion (Group 1, n = 14) and control (Group 2, n = 7) groups were identified. Blood samples at different times are collected from each rabbits. Blood samples from superior mesenteric artery occlusion group were taken 30 min after anesthesia but before laparotomy, 1, 2, and 3 h after superior mesenteric artery ligation. Blood samples from control group were taken 1 h before, 1 and 3 h after anesthesia and laparotomy. The D-dimer and neopterin levels of each blood sample were measured. Results The probability of acute mesenteric ischemia was found to be 36 times higher when the D-dimer level was over 0.125 ng/L, whereas the probability was 19.2 times higher when the neopterin level was over 1.25 nmol/L. Conclusions In this experimental study, the combined elevation of two significant markers, D-dimer and neopterin, may be helpful for the early diagnosis of acute mesenteric ischemia.

scholarly journals Some blood values in cows diagnosed clinically as ephemeral fever

2012 ◽  
Vol 36 (0E) ◽  
pp. 190-193
Author(s):  
Jameel G. H.
Keyword(s):  
Statistical Analysis ◽  
Sodium Bicarbonate ◽  
Intravenous Injection ◽  
Serum Cholesterol ◽  
Control Group ◽  
Blood Samples ◽  
Cholesterol Levels ◽  
Bovine Ephemeral Fever ◽  
Low Levels

Sixty cows were included in this study which was conducted in Baquba for the period from April 2011 to October 2011. They were clinically diagnosed as having bovine ephemeral fever (BEF) infection. Blood samples were collected from infected cows before, after treatment, and after recovery. Additionally, 30 healthy cows were included as control group. Sera were separated and submitted for the determination of phosphorus, glucose, cholesterol and pH levels. Statistical analysis of biochemical values revealed significant decrease in serum cholesterol levels in infected cows as compared to control group (P<0.05). Furthermore, the diseased cows showed low levels of pH (acidosis), thus when they treated by intravenous injection of 5% sodium bicarbonate, the signs were rapidly subsided and the cows were completely recovered.

scholarly journals An experimental study of albino rats fed on Nigella sativa and aspirin for four weeks duration: determination of coagulation profile

2016 ◽  
Vol 10 (2) ◽  
Author(s):  
Akhtar Munir ◽  
Muhammad Tayyib ◽  
Muhammad Farooq ◽  
Muhammad Mashraf ◽  
Anjum Rashid ◽  
...  
Keyword(s):  
Experimental Study ◽  
Body Weight ◽  
Nigella Sativa ◽  
Control Group ◽  
Albino Rats ◽  
Clot Retraction ◽  
Blood Samples ◽  
Synthetic Diet ◽  
Group I

Ninety albino rats were selected and were divided into six groups on the basis of different diets given. Control group (I) was fed on synthetic diet and experimental groups (IIA, IIB, IIC, IID and IIE) were fed on 1 mg aspirin, 15mg, 30 mg, 45 mg Nigella Sativa per kg body weight respectively while HE was given 30 mg NS and 1 mg aspirin/kg body wt. Blood samples were collected by heart puncture and Coagulation parameters were done. BT was prolonged in groups taking aspirin only. APTT was reduced significantly in groups taking different concentration of NS when compared with control. Percentage of clot retraction was weak significantly in groups taking aspirin only when comparing with other groups.

scholarly journals DETERMINATION OF HEMATOLOGICAL PARAMETERS AND D-DIMER AMONG SUDANESE CHILDREN INFECTED WITH PLASMODIUM FALCIPARUM IN KHARTOUM STATE – SUDAN.

2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Fatima H. Abdallah ◽  
Abuzar Elnager ◽  
Safa Wdidi ◽  
Alneil M. Hamza ◽  
Abdalla E. Ali
Keyword(s):  
Plasmodium Falciparum ◽  
Platelet Count ◽  
Hematological Parameters ◽  
Hematological Parameter ◽  
Control Group ◽  
Healthy Children ◽  
Parameter Measuring ◽  
Hematological Changes ◽  
D Dimer

Background: Falciparum malaria is one of the causes of morbidity and mortality in third world , the pathogenesis of the infection results from the sequestration of infected hematological change in vital organs. Aim:  this study aim to measuring  hematological changes and  D- dimer among children affected with P falciparum. Methods: The total study group100 children, 60 as case study infected with Plasmodium Falciparum and 40 as control group healthy children. Hematological parameter measuring by hematological analyzer and D dimer measured by fluorescence Immunoassay used I. Chroma instrument. Results: Statistical analysis results of sixty children infected with malaria falciparum participated in the study the mean±SD had a significantly lower Hb (8.14gm/dl  ± 2.2) and haematocrit (24.4% ± 6.6) than control children (p <0.001), Thrombocytopenia was found in 59.3% of enrolled patients. Platelet count (98.53% ± 48.9) , D. dimer (7397.58 ng/ml ± 5867.07)  (p <0.001). D. dimer showed correlation with parasite density, Platelet count and Haematocrit correlated (r = 0.4, p <0.0001); (p <0.001). Conclusion: Malaria infection by plasmodium falciparum had major effects on hematological parameters such as thrombocytopenia , anemia and hyper coagulation   have been significantly associated with severity of malaria falciparum.  Keywords: Plasmodiu, Falciparum, D-dimer, Hematological parameters

Vergleich von hämostaseologischen Parametern im arteriellen und venösen Blut bei Patienten mit peripherer arterieller Verschlußkrankheit

VASA ◽  
1999 ◽  
Vol 28 (1) ◽  
pp. 10-14 ◽  
Author(s):  
Woller ◽  
Lawall ◽  
Amann ◽  
Angelkort
Keyword(s):  
Venous Blood ◽  
Arterial System ◽  
Coagulation Activation ◽  
Blood Samples ◽  
Coagulation Parameters ◽  
Arterial Blood ◽  
Artery Disease ◽  
Peripheral Arterial ◽  
D Dimer

Background: Several studies proved the co-existence of peripheral arterial occlusive disease (PAOD) and hypercoagulability. However, in practice coagulation parameters are mainly determined from venous blood samples. In this study several coagulation parameters in arterial and venous blood were examined for differences and the validity of coagulation parameters determined in venous blood was investigated. Patients and methods: In 22 patients with peripheral artery disease venous and arterial blood samples from vessels of the diseased leg were examined for the concentration of thrombine-antithrombine III-complex (TAT), prothrombin fragments (F1 and F2) and D-dimers, and results were compared. Results: Mean concentrations of TATs and prothrombin fragments F1 and F2 were significantly higher in arterial than in venous blood. TAT-complex was the most sensitive parameter for quantification of thrombin generation. D-dimer levels did not differ in arterial and venous blood. TAT and F1 and F2 concentrations in arterial and venous blood did not correlate in individual patients whereas D-dimer concentration did. Conclusion: The determination of TAT and F1+F2 in venous blood does not adequately reflect the degree of the local coagulation activation in the arterial system. As indicators for hypercoagulability, D-Dimer values are less sensitive than F1+2, but venous D-dimer concentrations mirror arterial levels.

scholarly journals Comparative Study of Coagulation Profile in Benign and Malignant Neoplasms in Bhopal, India

2021 ◽  
Vol 10 (22) ◽  
pp. 1662-1666
Author(s):  
Rajni Choudhary ◽  
Seema Jathapi ◽  
Nigam R. K. ◽  
Reeni Malik ◽  
Ravi Kumar Meena
Keyword(s):  
Platelet Count ◽  
Comparative Study ◽  
Complete Blood Count ◽  
Morbidity And Mortality ◽  
Control Group ◽  
Malignant Neoplasms ◽  
Coagulation Parameters ◽  
Thrombotic Complications ◽  
Key Words Coagulation ◽  
D Dimer

BACKGROUND Thrombosis is a well-recognized and common complication in patients with malignant disease and can contribute significantly to the morbidity and mortality of this disease. We wanted to compare the coagulation parameters in benign and malignant neoplasms. METHODS A comparative study was done and a total of 230 cases (75 benign and 155 malignant) and 120 controls were evaluated. After processing the blood samples, various tests like complete blood count, platelet count, prothrombin time (PT), activated partial thromboplastin time (APTT), fibrin degradation product (FDP), and D-Dimer were done. RESULTS Significant differences were noted in the coagulation parameters of both the groups. Malignant cases show abnormal coagulation parameters as compared to control group. Mean platelet count - control - 250 +/- 61, cases - 375 +/- 100, mean PT – control - 14.1 +/- 6 s, cases - 15.2 +/- 1.2 s, mean APTT- control - 29.5 +/- 1.3 s, cases - 34.8 +/- 2.5 s. CONCLUSIONS Cancer is associated with a high risk of thrombotic complications. Altered coagulation parameters significantly correlate with malignant nature of tumour and also their spread. Preventing this complication is clinically relevant because it significantly contributes to morbidity and mortality in these patients. KEY WORDS Coagulation, Neoplasm, FDP, D-Dimer, Thrombosis

scholarly journals Some indicators of hemostasis and mortality from thrombotic complications in patients with CKD stage VD who were treated by programmed hemodialysis (five-year follow-up)

2021 ◽  
Vol 25 (3) ◽  
pp. 409-412
Author(s):  
O. B. Storozhuk ◽  
I. B. Seleznyova ◽  
L. O. Storozhuk ◽  
T. M. Platonova ◽  
B. G. Storozhuk ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Protein C ◽  
Control Group ◽  
Strong Positive Correlation ◽  
General Group ◽  
Soluble Fibrin ◽  
Ckd Stage ◽  
Thrombotic Complications ◽  
D Dimer

Annotation. Thrombotic complications in patients with stage VD chronic kidney disease (CKD), treated by program hemodialysis is one of the causes of high mortality in this category of patients; its major pathogenetic component proved to be hemostatic system disturbances characterized by systemic activation of blood clotting process leading to the development of thrombophilia. Objective – to study the biochemical molecular markers of hemostasis in patients with stage VD CKD, treated by program hemodialysis, and to determine their long-term effect on the development of thrombotic complications and mortality. The study included 88 patients (52 males and 36 females) aged 26 to 65 years with stage VD CKD, treated by program hemodialysis. The patients were followed up for five years. Soluble fibrin (sF) level was determined by two-site enzyme-linked immune-sorbent quantitative assay; D-dimer – by enzyme immunoassay using specific monoclonal antibodies to D-dimer epitopes; protein C (pC) activity in blood plasma was estimated by its activation with copperhead snake venom followed by spectrophotometry with wavelength 405 nm. Blood plasma fibrinogen was determined using thrombin-like enzyme Antsistron-H by spectrophotometry with wavelength 280 nm. Blood plasma fibrinolytic activity was evaluated by the relationship between D-dimer and soluble fibrin. Processing of materials was carried out using the methods of variation statistics and correlation analysis. During five years of follow up there were 13 deaths (14.8%), 7 among males and 6 among females, caused by thrombotic complications. The main thrombotic complications were myocardial infarction (6), ischemic stroke (4), mesenteric thrombosis (2) and disseminated intravascular coagulation syndrome (DIC) (1) (according to pathomorphological data). The patients of general group were found to have significantly increased sF level, decreased pC as compared to the control group, as well as twofold increase of fibrinogen concentration along with decreased D-dimer/sF ratio and no response of D-dimer to increased soluble fibrin level. The tendency of D-dimer/sF ratio to increase in those who died because of thrombotic complications could be indicative of microthrombosis with formation of fibrin derivatives along with mild activation of fibrinolysis. Correlation relationships between soluble fibrin and D-dimer, fibrinogen and protein C in general group were assessed, and the following data were obtained: medium direct relationship between soluble fibrin and D-dimer (r= 0.56) (p<0.001), and absence of correlation with fibrinogen (r= -0.12) and protein C (r= -0.10) (p˃0.1). Besides, strong positive correlation was demonstrated between D-dimer and soluble fibrin (r= 0.87) (p<0.001), and moderately negative one between D-dimer and protein C (r= -0.21) (p<0.05). It should be noted that in patients with thrombotic complications, positive correlations between soluble fibrin and D-dimer become stronger (r= 0.51) (p<0.001), as well as negative ones between soluble fibrin and protein C (r= -0.22) (p<0.05). Depressive state of anticoagulant component along with activation of coagulation factors are supposed to be one of the indicators predicting thrombophilia in this category of patients.

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