Clinical and diagnostic value of the method of chromato-mass spectrometry of microbial markers in case of damage to the oral mucosa in children by rheumatic diseases

2021 ◽  
Vol 1 (38) ◽  
pp. 49-57
Author(s):  
A. A. Skakodub ◽  
O. I. Admakin ◽  
Ad. A. Mamedov ◽  
N. A. Geppe ◽  
A. V. Simonova
Keyword(s):  
Mass Spectrometry ◽  
Oral Mucosa ◽  
Rheumatic Diseases ◽  
Modern Method ◽  
Diagnostic Value ◽  
Term Treatment ◽  
Individual Treatment ◽  
Oral Microbiota ◽  
Long Term Treatment ◽  
Wide Range

Due to the presence of a large percentage of 42.6% secondary oral infection in children with rheumatic diseases [1, 2], which arose during long-term treatment of shock and maintenance doses of anti-inflammatory therapy, it was important to study the microbiota [16, 17]. This paper for the first time applied a modern method for assessing the microbiota of various biotopes of the affected oral mucosa in children with rheumatic diseases – chromatosis-mass-spectrometry (CMSM), based on the quantitative determination of the level of markers of microorganisms: fatty acids, aldehydes, alcohols [5, 7, 10, 11]. СMSM is a highly sensitive method with a wide diagnostic spectrum. The study of a wide range of microorganisms provides new opportunities in the diagnosis of oral dysbacteriosis and increasing the effectiveness of individual treatment. The aim of the study is to improve the level of diagnosis and treatment of oral mucosal diseases in children with rheumatic diseases, through the use of chromato-mass-spectrometry of the oral microbiota.

scholarly journals Achieving Viral Suppression in 90% of People Living With Human Immunodeficiency Virus on Antiretroviral Therapy in Low- and Middle-Income Countries: Progress, Challenges, and Opportunities

2018 ◽  
Vol 66 (10) ◽  
pp. 1487-1491 ◽  
Author(s):  
Jean B Nachega ◽  
Nadia A Sam-Agudu ◽  
Lynne M Mofenson ◽  
Mauro Schechter ◽  
John W Mellors
Keyword(s):  
Human Immunodeficiency Virus ◽  
Viral Suppression ◽  
Cost Effective ◽  
Term Treatment ◽  
Middle Income ◽  
Middle Income Countries ◽  
Long Term Treatment ◽  
Wide Range ◽  
Immunodeficiency Virus ◽  
Low And Middle Income

Abstract Although significant progress has been made, the latest data from low- and middle-income countries show substantial gaps in reaching the third “90%” (viral suppression) of the UNAIDS 90-90-90 goals, especially among vulnerable and key populations. This article discusses critical gaps and promising, evidence-based solutions. There is no simple and/or single approach to achieve the last 90%. This will require multifaceted, scalable strategies that engage people living with human immunodeficiency virus, motivate long-term treatment adherence, and are community-entrenched and ‑supported, cost-effective, and tailored to a wide range of global communities.

scholarly journals GENETIC POLYMORPHISM OF RETROPERITONEAL MYXOID LIPOSARCOMA

2020 ◽  
Vol 19 (3) ◽  
pp. 89-96
Author(s):  
A. Yu. Volkov ◽  
V. M. Safronova ◽  
S. N. Nered ◽  
L. N. Lyubchenko ◽  
I. S. Stilidi
Keyword(s):  
Molecular Genetic ◽  
Myxoid Liposarcoma ◽  
Molecular Genetic Analysis ◽  
Term Treatment ◽  
Missense Mutations ◽  
Synonymous Mutations ◽  
Erbb2 Gene ◽  
Long Term Treatment ◽  
Wide Range ◽  
Next Generation Sequencing Ngs

Objective: to detect new molecular genetic markers and therapeutic targets in retroperitoneal myxoid liposarcoma.Material and Methods. DNA samples isolated from tumor tissue and obtained from formalinfixed paraffin-embedded (FFPE) slides were used. DNA was extracted using the GeneRead DNA FFPE Kit (50) (Qiagen). High-throughput next generation sequencing (NGS) using the GeneReader Actionable Insights Tumor Panel (GRTP – 101X) on the QCI Analyzer version 1.1 platform (Qiagen) was used for molecular genetic analysis of 12 genes involved in carcinogenesis: KRAS, NRAS, KIT, BRAF, PDGFRA, ALK, EGFR, ERBB2, PIK3CA, ERBB3, ESR1, RAF1.Results. Targeted sequencing of retroperitoneal extra-organ myxoid liposarcoma demonstrated genetic heterogeneity. Our study was the first to describe mutations and polymorphic variants in genes, such as EGFR, PIK3CA, ALK, BRAF, ERBB2 / 3, ESR1, KIT, PDGFRA in myxoid liposarcoma.Conclusion. This study demonstrated a wide range of molecular genetic rearrangements in retroperitoneal extra-organ myxoid liposarcoma. Synonymous mutations in the EGFR (Q787Q) and PDGFRA (P567P) genes were detected in all cases (100 %). Missense mutations in the ERBB2 gene (P1170A) and synonymous mutations in the ALK (G845G) and BRAF genes were identified in 75 % of cases. Missense mutation in the PIK3CA gene (I391M) was detected in 25 % of cases. The gene polymorphisms presented in this paper are most likely involved in the carcinogenesis of retroperitoneal myxoid liposarcoma. Further studies with larger patient groups and multivariate analysis of long-term treatment results are required. 

scholarly journals Effect of interleukin-6, -17, -21, -22, and -23 and STAT3 on signal transduction pathways and their inhibition in autoimmune arthritis

2021 ◽  
Author(s):  
Izabela Woś ◽  
Jacek Tabarkiewicz
Keyword(s):  
Rheumatic Diseases ◽  
Current Knowledge ◽  
Early Stage ◽  
Bone Destruction ◽  
Term Treatment ◽  
Inflammatory Factors ◽  
Autoimmune Arthritis ◽  
Preventative Measures ◽  
Long Term Treatment ◽  
Anti Inflammatory

AbstractRheumatic diseases are complex autoimmune diseases which include among others rheumatoid arthritis (RA), juvenile idiopathic arthritis (JIA), and psoriatic arthritis (PsA). These diseases are characterized by prolonged and increased secretion of inflammatory factors, eventually leading to inflammation. This is often accompanied by persistent pain and stiffness in the joint and finally bone destruction and osteoporosis. These diseases can occur at any age, regardless of gender or origin. Autoimmune arthritis is admittedly associated with long-term treatment, and discontinuation of medication is associated with unavoidable relapse. Therefore, it is important to detect the disease at an early stage and apply appropriate preventative measures. During inflammation, pro-inflammatory factors such as interleukins (IL)-6, -17, -21, -22, and -23 are secreted, while anti-inflammatory factors including IL-10 are downregulated. Research conducted over the past several years has focused on inhibiting inflammatory pathways and activating anti-inflammatory factors to improve the quality of life of people with rheumatic diseases. The aim of this paper is to review current knowledge on stimulatory and inhibitory pathways involving the signal transducer and activator of transcription 3 (STAT3). STAT3 has been shown to be one of the crucial factors involved in inflammation and is directly linked with other pro-inflammatory factors and thus is a target of current research on rheumatoid diseases.

EFFECTS OF LONG-TERM TREATMENT AND OF LOW CONCENTRATIONS OF THYROID HORMONES ON THE OXYGEN UPTAKE OF TOAD TISSUES

1966 ◽  
Vol 36 (3) ◽  
pp. 221-229 ◽  
Author(s):  
C. C. THORNBURN ◽  
A. J. MATTY
Keyword(s):  
Oxygen Consumption ◽  
Oxygen Uptake ◽  
Bufo Bufo ◽  
Term Treatment ◽  
Seasonal Effect ◽  
Long Term Treatment ◽  
Wide Range ◽  
Two Peaks

SUMMARY Isolated tissues of the toad Bufo bufo (urinary bladder, skin and kidney) were treated in vitro with thyroxine (T4) or tri-iodothyronine (T3) both with and without prior administration of the hormones in vivo. They were incubated at low temperature, and their oxygen consumption studied for 48 hr. Both hormones had little effect in vitro. Pretreatment with T4 in vivo usually lowered oxygen uptake; the effect of pretreatment with T3 varied. The presence of alanine in the incubating medium caused a marked increase in oxygen consumption. A seasonal effect was also found. The respiration of isolated toad bladder was stimulated by a wide range of concentrations of thyroxine in vitro, and there were two peaks of stimulation.

scholarly journals Role of hyaluronic acid in the prevention and treatment of radiation-induced oropharyngeal mucositis

2019 ◽  
Vol 9 (3) ◽  
pp. 29-37
Author(s):  
Yu. V. Alymov ◽  
А. М. Mudunov ◽  
S. B. Alieva ◽  
А. V. Ignatova ◽  
R. R. Kaledin ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Adverse Events ◽  
Term Treatment ◽  
Universal Method ◽  
Long Term Treatment ◽  
Wide Range ◽  
Prevention And Treatment ◽  
Radiation Induced ◽  
Oropharyngeal Mucositis

One of the earliest and most common adverse events of radiotherapy in patients with oropharyngeal cancers is mucositis. Radiation-induced mucositis significantly impairs the quality of life, patient compliance, and sometimes even long-term treatment outcomes. Currently, there is a wide range of drugs and techniques for the prevention and treatment of this complication. Nevertheless, these methods are not always highly effective and often have their own adverse events; therefore, the search for a universal method still continues. The present review aims to analyze the efficacy of hyaluronic acid in the prevention and treatment of oropharyngeal mucositis.

Prevalence of herpes simplex virus I, II, cytomegalovirus, Epstein–Barr virus and human papillomavirus (6, 11, 16 and 18) in normal oral mucosa and OSCC patients in Saint-Petersburg (Russia).

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e18539-e18539
Author(s):  
Svetlana Kutukova ◽  
Natalia P. Beliak ◽  
Julia V. Ivaskova ◽  
Alexey B. Chuhlovin ◽  
Andrey I. Yaremenko ◽  
...  
Keyword(s):  
Healthy Volunteers ◽  
Oral Mucosa ◽  
Normal Mucosa ◽  
Term Treatment ◽  
Viral Dna ◽  
Normal Oral Mucosa ◽  
Barr Virus ◽  
Radical Treatment ◽  
Long Term Treatment ◽  
Simplex Virus

e18539 Background: The objective of our study was to evaluate the prevalence of DNA of HSV I, II, CMV, EBV, HPV6.11, HPV16, HPV18 in normal oral mucosa and oral squamous cell carcinoma (OSCC), and determine this association with long-term treatment outcomes and patient survival. Methods: The study included 116 patients with OSCC (stage I-IVB who received standard treatment) and 50 healthy volunteers, median age 60.0 yrs (95% CI 58.0-64.0). Using real-time PCR (polymerase chain reaction), freshlyfrozen samples of the tumor and normal mucous membranes of the cavity were analyzed. mouths, which, after collection, were placed in an EDTA solution and frozen at 20°C. Results: In 54 (46.55%) OSCC and 17 (34.00%) normal mucous viral DNA was not found (p = 0.13). The occurrence of DNA (OSCC vs. normal) of the following viruses was the same in both OSCC and normal mucosa: HSV I, II: 2 (1.72%) vs. 2 (4.00%) (p = 0.38); EBV: 40 (34.48%) vs. 15 (30.00%) (p = 0.57); CMV: 5 (4.31%) vs. 2 (4.00%) (p = 0.93); HPV6.11: 16 (13.79%) vs. 6 (15.00%) (p = 0.84); HPV16: 12 (10.34%) vs. 2 (4.00%) (p = 0.18). HPV18 DNA: 20 (17.24%) vs. 24 (48.0%) (p <0.0001) was found significantly less frequently in the OSCC group. In addition, EBV + HPV18: 7 (14.00%) vs. 5 (4.31%) (p = 0.0270) was more common vs. OSCC in the group of healthy volunteers; CMV + HPV18: 2 (4.00%) vs. 0 (p = 0.0302). In the OSCC group, patients with stage III disease were more often virus-positive (p = 0.0137); the pterygo-maxillary fold region (p = 0.0438); with partial keratinization of cells (p = 0.05). PFS in the HPV18 (+) OSCC group was 38.5 months (95% CI 11.00-66.0) and 23.5 m higher than the group of HPV18 (-) patients (HR = 0.63; 95% CI 0.39-1.03; p = 0.0399). In virus-negative patients, PD was more often recorded: 12 (24.00%) vs. 2 (3.03%) (p = 0.0006). In the group of virus-positive patients, OS and PFS, even with non-radical treatment, were significantly higher than virus-negative patients with radical treatment. OS: vir (+)/non-radical = 69.0 mo vs. vir (-)/radical = 15.0 mo (HR = 0.22; 95% CI 0.11-0.47; p <0.0001). PFS: vir (+)/non-radical = 56.0 mo vs. vir (-)/radical = 11.0 mo (HR = 0.20; 95% CI 0.09-0.45; p <0.0001). Conclusions: The association of OSCC with viral DNA, especially HPV18, is a favorable prognosis of the disease and a longer OS and PFS, even when radical treatment is not possible.

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