scholarly journals Perbandingan Susu Sapi Perah Pada Pemerahan Pagi dan Sore Terhadap Total Plate Count dan Colioform di KUD Gemah Ripah Sukabumi Jawa Barat

2020 ◽  
Vol 7 (1) ◽  
pp. 65
Author(s):  
Raden Febrianto Christi ◽  
Didin Supriat Tasripin ◽  
Dwi Suharwanto ◽  
Eka Wulandari
Keyword(s):  
Dairy Cows ◽  
Dairy Cow ◽  
Research Method ◽  
Cow Milk ◽  
Plate Count ◽  
Lactation Period ◽  
Nutritional Content ◽  
Milk Samples ◽  
Total Plate Count ◽  
Paired T Test

ABSTRAKSusu adalah cairan putih yang keluar dari ambing sapi perah yang memiliki kandungan gizi yang baik. Kualitas susu sangat dipengaruhi oleh beberapa faktor salah satunya adalah Total Plate Count dan Colioform. Tujuan penelitian ini adalah untuk mengetahui perbandingan  susu sapi perah pada pemerahan pagi dan sore terhadap TPC dan Colioform di KUD Gemah ripah, Sukabumi. Metode penelitian yang digunakan adalah uji t berpasangan dengan sampel ternak sapi perah 14 ekor yang masing-masing diulang sebanyak 7 ekor dengan masa laktasi sama. Sampel susu diambil 100ml dari setiap ekor dengan waktu pemerahan berbeda kemudian dimasukkan pada coolbox untuk dilakukan pengujian nilai TPC dan Colioform di Laboratoium. Hasil penelitian menunjukkan bahwa TPC dan colioform pada pemerahan susu pagi hari menghasilkan pengaruh nyata (P<0,05) dibandingkan pada susu hasil pemerahan sore yaitu 1,1 x 106 CFU/ml dan 6985,714 CFU/mL. Kesimpulan menunjukkan bahwa TPC dan colioform pada susu pada berbagai waktu pemerahan yang berada di KUD gemah ripah, sukabumi termasuk pada kualitas yang premium jika dibandingkan dengan kualitas SNI.Kata kunci : colioform, pemerahan pagi sore, susu sapi perah, total plate count,ABSTRACKMilk is a white liquid that comes out of a dairy cow's udder which has good nutritional content. Milk quality is strongly influenced by several factors, one of which is the Total Plate Count and Coloform. The purpose of this study was to determine the comparison of milk from dairy cows in the morning and evening milking of TPC and Colioform in Kemah Gemah ripah, Sukabumi. The research method used was paired t test with samples of 14 dairy cows, each of which was repeated as many as 7 with the same lactation period. Milk samples were taken 100ml from each tail with different milking times and then put in a coolbox for testing the value of TPC and Coloform in Laboratory. The results showed that TPC and colioform in milking in the morning produced a significant effect (P<0.05) compared to milk produced by afternoon milking namely 1.1 x 106 CFU / ml and 6985.714 CFU / mL. The conclusions show that TPC and colioform in milk at various milking times in KUD Gemah ripah, sukabumi are included in premium quality when compared to SNI quality.Keywords: colioform, dairy cow milk, milking morning afternoon, total plate count

scholarly journals Bacteriological quality and safety of raw cow milk in Madurai (South India)

2013 ◽  
Vol 48 (2) ◽  
pp. 109-114 ◽  
Author(s):  
S Lingathurai ◽  
P Vellathurai
Keyword(s):  
Microbiological Quality ◽  
Raw Milk ◽  
Cow Milk ◽  
Coliform Bacteria ◽  
Plate Count ◽  
Quality And Safety ◽  
E Coli ◽  
Milk Samples ◽  
Total Plate Count ◽  
The Mean

The microbiological quality and safety of raw milk from 60 dairy farms in Madurai were determined. Milk samples were collected at 60 centers from four regions, namely northern, eastern, western and southern (NEWS) according to stratified random sampling design. Samples were analyzed for Total plate count (TPC), psychrotrophs, thermophiles, Staphylococcus aureus, coliform, Escherichia coli 0157: H7 and Salmonella. The mean counts per ml for TPC, psychrotrophs and thermophiles were 12.5x106, 5x103 and 6.85x103 respectively. From the 60 milk samples tested, coliform bacteria contaminated approximately 90% and 70% were E. coli positive, with mean counts ranged from 103 to 104 cfu ml-1. S. aureus was isolated from more than 61.7% of the samples and the mean count per ml was 6.2x103. Meanwhile, E. coli 0157: H7 was also detected in 39 (65%) samples. However, Salmonella was only detected in 8 (13.3%) of the samples with the southern region having the highest frequency of isolation. Bangladesh J. Sci. Ind. Res. 48(2), 109-114, 2013 DOI: http://dx.doi.org/10.3329/bjsir.v48i2.15741

scholarly journals STATUS MIKROBIOLOGI (Total Plate Count dan Staphylococcus aureus) SUSU SAPI PERAH DI KABUPATEN TANGGAMUS PROVINSI LAMPUNG

2020 ◽  
Vol 4 (3) ◽  
pp. 197-204
Author(s):  
Oktora Luhur Handika ◽  
Veronica Wanniatie ◽  
Purnama Edy Santosa ◽  
Arif Qisthon
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Cows ◽  
Plate Count ◽  
Survey Method ◽  
Milk Samples ◽  
Fresh Milk ◽  
Census Methods ◽  
Total Plate Count ◽  
And Staphylococcus Aureus ◽  
Microbiological Status

This study aimed to determine the microbiological status (Total Plate Count and Staphylococcus aureus) of milk of dairy cows of people's farms. Research location was in Tanggamus Regency, Lampung Province. This research used survey method with census methods. Fresh milk samples were taken during milking in the morning, which is as much as 250 ml from all lactating cows. In addition, this study also used questionnaire to know the condition of the dairy cows. Nineteen milk samples were analyzed at the Veterinary Public Health Laboratory of Lampung Veterinary Center, Bandar Lampung. Analysis of milk microbiological status was based on Total Plate Count and Staphylococcus aureus. The results of this study indicated that 100% of milk samples had Total Plate Count and Staphylococcus aureus values ??below the maximum SNI number of 3141.1: 2011. Milk of dairy cow in Tanggamus Regency, Lampung Province is feasible and safe for consumption. Keywords: Dairy cows, Microbiological status, Milk.

Relationships among macro-minerals, other selected serum markers of bone profile and milk components of dairy cows during late-lactation

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Ryszard Mordak ◽  
Zbigniew Dobrzański ◽  
Robert Kupczyński
Keyword(s):  
Dairy Cows ◽  
Milk Fat ◽  
Dry Matter ◽  
Inorganic Phosphorus ◽  
Serum Markers ◽  
Laboratory Diagnostics ◽  
Lactation Period ◽  
Milk Samples ◽  
Milk Components ◽  
Macro Mineral

AbstractTesting blood and milk parameters as well as analysing the relationships among these markers is very useful for monitoring the internal homeostasis and health in high-yielding dairy cows during various production periods. The aim of the study was to assess the correlations (relationships) among macro-minerals, such as calcium (Ca), inorganic phosphorus (P), magnesium (Mg), other selected bone profile markers, such as total protein (TP), albumin, activity of alkaline phosphatase (ALP) measured in serum and selected milk components such as number of somatic cells (SCC), colony-forming units (CFU), milk fat (MF), milk protein (MP), milk lactose (ML), dry matter (DM), non-fat dry matter (FDM) and milk production in late-lactation cows. Both blood and milk samples were collected from 11 clinically healthy milking cows during the late-lactation period. The cows were examined once a day for 3 consecutive days resulting in 33 sets of blood and milk samples for laboratory and statistical analysis. Significant correlations were observed between: Mg and MP, Mg and FDM, ALP and SCC, TP and SCC, TP and MP, TP and FDM, albumin and MP, albumin and FDM, P and Mg, Mg and albumin, and between TP and albumin. When monitoring macro-mineral homeostasis and mammary gland health, especially in intensively fed high-yielding dairy cows correlations between these markers should be considered. The revealed correlations can allow for deeper comparative laboratory diagnostics of homeostasis and can be especially useful for laboratory monitoring of the potential risk of subclinical macro-mineral deficiency in high-yielding dairy cows.

Withdrawal of cefoperazone with milk after intramammary administration in dairy cows – prospective and retrospective analysis

2017 ◽  
Vol 20 (2) ◽  
pp. 261-268
Author(s):  
A. Burmańczuk ◽  
T. Grabowski ◽  
T. Błądek ◽  
C. Kowalski ◽  
P. Dębiak
Keyword(s):  
Dairy Cows ◽  
Half Life ◽  
Drug Distribution ◽  
Compartment Model ◽  
Clinical Mastitis ◽  
Pharmacokinetic Analysis ◽  
Pharmacokinetic Parameters ◽  
Lactation Period ◽  
Milk Samples ◽  
Drug Concentrations

Abstract The aim of the study was to carry out retrospective and prospective comparative analyses of the pharmacokinetics of CEF after single intramammary (IMM) administration in cows. The prospective study (study A) was conducted on 9 dairy cows of the Polish Black-White race with clinical mastitis during the lactation period. Milk samples were collected at 2, 4, 6, 8, 10, 24, 36, 48, 72 and 84 h after single IMM administration of 250 mg of CEF to one quarter. Drug concentrations in milk samples were determined by HPLC-MS/MS technique and the results of the pharmacokinetic analysis were compared to those obtained in previous studies based on the microbiological (study B) and HPLC-UV methods (study C and D). Pharmacokinetic parameters were calculated based on adapted two-compartment model of drug distribution. One of the findings of the comparison of the analysed investigations is that the CEF kinetics determined with the microbiological method is consistent with the results obtained by the authors of this paper. Both studies yielded similar results of the key pharmacokinetic parameters related to the level of the drug distribution to tissues and elimination half-life. In the pharmacodynamic analysis, the observations in all four studies were entirely consistent and have shown lower values of T>MIC90 in healthy animals and significantly higher values in infected dairy cows. The comparison of studies A, B, C, and D revealed that the time of complete CEF wash-out of 90.90% varied and amounted to 5.7, 8.0, 2.2, and 2.2 days after administration of the drug, respectively. It was confirmed that not only the type of the analytical method but also correct sampling have a significant impact on determination of the correct value of the drug half-life after IMM administration. The comparative analysis of studies in which the milk yield was high and low allows a conclusion that this parameter in the case of CEF has no significant effect on T>MIC90.

scholarly journals Identifikasi Sakarin, Siklamat, dan Natrium Benzoat Serta Karakteristik Susu Kedelai yang Dijual di Pasar Tradisional Wilayah Jimbaran, Bali Selama Penyimpanan

2021 ◽  
Vol 10 (1) ◽  
pp. 141
Author(s):  
Novelita Olivea Herman ◽  
Ni Luh Ari Yusasrini ◽  
I Nengah Kencana Putra
Keyword(s):  
Sodium Benzoate ◽  
Sampling Technique ◽  
Plate Count ◽  
Survey Method ◽  
Soy Milk ◽  
Sensory Assessment ◽  
Traditional Markets ◽  
Milk Samples ◽  
Total Plate Count

This study aims to identify saccharin, cyclamate, and sodium benzoate in soy milk circulating in traditional markets in the Jimbaran region, determine the percentage of soy milk samples in traditional markets in the Jimbaran region that contain saccharin, cyclamate, and sodium benzoate, and determine the characteristic of milk soybeans in the traditional markets of the Jimbaran region, Bali during storage. This study uses a survey method with saturation sampling technique. Saccharin, cyclamate and sodium benzoate were analyzed qualitatively and quantitatively while the characteristic of soy milk were observed included pH, total plate count, and sensory assessment. Saccharin, cyclamate, sodium benzoate, pH, total plate count, and sensory assessment were analyzed using descriptive methods. The results showed that soy milk in traditional markets in the Jimbaran region did not contain saccharin, 80% used cyclamate, 100% used sodium benzoate, and characteristics of soy milk in traditional markets in Jimbaran, Bali during 8 hours storage has pH between 6.8 to 8.34, as much as 70% of soy milk has total plate count less than 106 CFU/ml, and it is still well consuming because the color has the like criteria, aroma and flavor has the neutral criteria and very not acidic.

scholarly journals PROKSIMAT DAN TOTAL BAKTERI IKAN LAYANG (Decapterus sp.) ASIN KERING HASIL PENGERINGAN MENGGUNAKAN PENGERING SURYA TERTUTUP

2021 ◽  
Vol 1 (1) ◽  
pp. 29-39
Author(s):  
Raja B. D. Sormin ◽  
Edir Lokollo ◽  
Febe F. Gaspersz ◽  
Vicko F. J. Tahalea
Keyword(s):  
Moisture Content ◽  
Protein Content ◽  
Salt Solution ◽  
Research Method ◽  
Ash Content ◽  
Plate Count ◽  
Solar Drying ◽  
Drying Method ◽  
Salted Fish ◽  
Total Plate Count

This study aims to determine the proximate and total bacterial of dry salted scad fish (Decapterus sp.)dried using a closed solar drying sistem. The research method begins with fish weed in ‘butterfly’ thenwashed using flowed water and immersed in 15% salt solution for 30 minutes. The drying method was aclosed sun drying cabinet sistem, with 3 drying racks named Rack 1, Rack 2 and Rack 3 starting from thebottom. The dried salted fish was analyzed its proximate consisting of moisture content, ash content, fatcontent and protein content, as well as bacterial tests using Total Plate Count (TPC). The results showedthat the proximate values on Rack 1, Rak2, and Rack 3 were water content of 25.58%, 20.39%, and17.80% respectively; ash content of 9.64%, 9.69% and 7.71% respectively, fat content 8.08%, 11.40%and 15.16%, respectively and protein content 53.73%, 58.10%, and 59.11%. respectively. Meanwhile,the total plate count (TPC) value of dried salted layang fish (Decapterus sp) on Rack 1, Rak2, and Rack 3was 2.73 log x CFU / g or 5.4 x 102 CFU / g, 2.74. logx CFU / g or 5.5 x 102 CFU / g, and 2.74 logx CFU / gror 5.9 x 102 CFU / gr, respectively. The best place to put fish in the dryer is on Rack 3 as this is indicatedby the low moisture content. The TPC value was still within the value limit required by the SNI.

scholarly journals Study of aflatoxins incidence in cow feed and milk in Serbia during 2013.

2015 ◽  
Vol 69 (6) ◽  
pp. 651-656 ◽  
Author(s):  
Danka Spiric ◽  
Jasna Djinovic ◽  
Vesna Jankovic ◽  
Branko Velebit ◽  
Tatjana Radicevic ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Aflatoxin B1 ◽  
Negative Impact ◽  
Raw Milk ◽  
Self Control ◽  
Cow Milk ◽  
Aflatoxin M1 ◽  
Climate Conditions ◽  
Milk Samples ◽  
Beet Pulp

Atypical weather and climate conditions during the spring and summer 2012th were assumed to be the main reason for the aflatoxins contamination of corn crops in Serbia. High humidity in spring, and summer temperatures above the average contributed to the increased possibility of mycotoxins occurance in cereals in the fields. As a consequence, at the begining of 2013th contaminated corn used for dairy cows diet had negative impact on the safety of cow milk. The routine laboratory control data revealed an increased content of aflatoxin M1 in milk samples. Large number of raw milk and various feedstuff samples were collected from February to April 2013th and were analysed for the presence of aflatoxin M1 (AFM1) and aflatoxin B1 respectively. The collected samples were a part of enhanced self-control plans of the large dairy farms. Quantitative competitive and sandwich types of ELISA tests were used for the screening analysis of the feed and milk samples. Confirmation of the positive results obtained by ELISA tests was performed by UPLC-MS/MS method. Out of 281 samples of complete mixtures for dairy cows, 67 samples (24%) contained aflatoxin B1 quantities higher than the MRL of 0,005 mg/kg [4]. Corn samples were also tested for the presence of aflatoxin B1 revealing contamination of 22% above the MRL (0.03mg/kg). Aflatoxin M1 content in the 934 positive milk samples ranged from 0,005- 1,25 ?g/kg. The corresponding feed samples of sunflower meal, hay silage, corn silage and sugar beet pulp were screening negative, with the content of aflatoxin B1 less than 2 ?g/kg. The main source of aflatoxins were corn samples, wholemeal and feed mixtures derived from contaminated corn. The contamination peak was during March 2013, when 65% of milk samples contained amounts of aflatoxin M1 higher than 0,05 ?g/kg, and 13% of milk samples contained amounts higher than 0,5 ?g/kg.

Occurrence of Aflatoxin in Dairy Cow Feed Ingredients and Total Mixed Ration

2019 ◽  
Vol 35 (5) ◽  
pp. 679-686 ◽  
Author(s):  
Zeinab Mohammadi Shad ◽  
Mehrdad Ghavami ◽  
Griffiths G. Atungulu
Keyword(s):  
Dairy Cows ◽  
Dairy Cow ◽  
Rainy Season ◽  
Raw Milk ◽  
Farm Management ◽  
Average Level ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Feed Ingredients ◽  
Milk Samples

Abstract. The level of aflatoxin B1 (AFB1) in dairy cow feed ingredients and Total Mixed Rations (TMRs) procured at two farms for low- and high-yielding dairy cows were surveyed. Raw milk from the two groups of cows at each farm was sampled 24 h after feeding the cows with examined feedstuffs during both the rainy and the non-rainy season. The aflatoxin M1 (AFM1) level in the raw milk samples was measured 12-24 h later. The levels of AFB1 in feed and AFM1 in milk were determined by validated enzyme linked immunosorbent assay (ELISA). The influence of farm management and type of feeding system on aflatoxin occurrence were considered. AFB1 and AFM1 were detected in 100% of feed and milk samples, respectively. The average level of AFB1 in the feed ingredients and TMRs were in the range of 1.6-104.7 µg/kg and 11.0-56.0 µg/kg, respectively. The average level of AFM1 in milk samples was 77.0 ng/L. The average concentrations of AFB1 in feeds and AFM1 in milk procured in the rainy season were significantly greater than those procured in the non-rainy season (p&lt;0.05). Of the studied feed, maize silage was determined as the most contaminated feed ingredient in terms of AFB1 content. Furthermore, the AFM1 in 75% of milk samples obtained from high-yielding dairy cows and 25% of milk samples obtained from low-yielding dairy cows indicated AFM1 level higher than the maximum allowable Europe Commission limit of 50 ng/L. The results also showed that the occurrence of AFB1 in feed varied with farm feed management. The extent of translocation to AFMI in milk samples was dependent on type of cow, whether low- or high- milk yielding. This study suggests regular risk analysis and using good farm management practices are important to control aflatoxin contamination in feed and milk. Keywords: Aflatoxin B1, Aflatoxin M1, Dairy cows, Feed, Milk yield.

scholarly journals IDENTIFIKASI SAKARIN, SIKLAMAT, DAN NATRIUM BENZOAT SERTA KARAKTERISTIK SUSU KEDELAI YANG DIJUAL DI PASAR TRADISIONAL WILAYAH JIMBARAN, BALI SELAMA PENYIMPANAN

2020 ◽  
Vol 9 (4) ◽  
pp. 468
Author(s):  
Novelita Olivea Herman ◽  
NL. Ari Yusasrini ◽  
I Nengah Kencana Putra
Keyword(s):  
Sodium Benzoate ◽  
Sampling Technique ◽  
Plate Count ◽  
Survey Method ◽  
Soy Milk ◽  
Sensory Assessment ◽  
Traditional Markets ◽  
Milk Samples ◽  
Total Plate Count

This study aims to identify saccharin, cyclamate, and sodium benzoate in soy milk circulating in traditional markets in the Jimbaran region, determine the percentage of soy milk samples in traditional markets in the Jimbaran region that contain saccharin, cyclamate, and sodium benzoate, and determine the characteristic of milk soybeans in the traditional markets of the Jimbaran region, Bali during storage. This study uses a survey method with saturation sampling technique. Saccharin, cyclamate and sodium benzoate were analyzed qualitatively and quantitatively while the characteristic of soy milk were observed included pH, total plate count, and sensory assessment. Saccharin, cyclamate, sodium benzoate, pH, total plate count, and sensory assessment were analyzed using descriptive methods. The results showed that soy milk in traditional markets in the Jimbaran region did not contain saccharin, 80% used cyclamate, 100% used sodium benzoate, and characteristics of soy milk in traditional markets in Jimbaran, Bali during 8 hours storage has pH between 6.8 to 8.34, as much as 70% of soy milk has total plate count less than 106 CFU/ml, and it is still well consuming because the color has the like criteria, aroma and flavor has the neutral criteria and very not acidic.

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