Validation of in vitro digestion using simulated small intestinal fluid with specific digestive activity to predict the metabolizable energy of feed ingredients for duck

Anthocyanins are effective antioxidants but they have also been proposed to have other biological activities independent of their antioxidant capacities that produce health benefits. Examples range from inhibition of cancer cell growth in vitro, induction of insulin production in isolated pancreatic cells, reduction of starch digestion through inhibition of a-glucosidase activity, suppression of inflammatory responses as well as protection against age-related declines in cognitive behavior and neuronal dysfunction in the central nervous system. However, to achieve any biological effect in a specific tissue or organ, anthocyanins must be bioavailable; i.e. effectively absorbed from the gastrointestinal tract (GIT) into the circulation and delivered to the appropriate location within the body. In this study, we assess the stability of anthocyanins from commercial Black currant (Ribes nigrum L.) juice using an in vitro digestion procedure that mimics the physiochemical and biochemical conditions encountered in the gastrointestinal tract (GIT). The main objective of this work was the evaluation of stability of anthocyanins during in vitro digestion in gastric and intestinal fluid regarding whether appropriate enzyme (pepsin or pancreatin) was added or not. Anthocyanins present in commercial black currant juice remain stable during in vitro digestion in gastric fluid regardless whether pepsin was added into the medium or not. Also, they remain stable during in vitro digestion in simulated intestinal fluid without pancreatin. The stability studies of anthocyanins in the intestinal fluid containing pancreatin indicated reduced stability, which also mainly contribute to slight reduction of total anthocyanins content (1,83%-) in commercial black currant juice.

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Accuracy of predicting metabolizable energy from in vitro digestible energy determined with a computer-controlled simulated digestion system in feed ingredients for ducks

Animal Nutrition ◽

10.1016/j.aninu.2021.04.002 ◽

2021 ◽

Author(s):

Yuming Wang ◽

Liting Yin ◽

Hu Zhang ◽

Ke Li ◽

Dailin Li ◽

...

Keyword(s):

Metabolizable Energy ◽

Feed Ingredients ◽

Digestible Energy ◽

Simulated Digestion ◽

Computer Controlled

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Physico-Chemical Characteristics and In Vitro Gastro-Small Intestinal Digestion of New Zealand Ryegrass Proteins

Foods ◽

10.3390/foods10020331 ◽

2021 ◽

Vol 10 (2) ◽

pp. 331

Author(s):

Lovedeep Kaur ◽

Harmandeepsingh Lamsar ◽

Ignacio F. López ◽

Manon Filippi ◽

Dayna Ong Shu Min ◽

...

Keyword(s):

New Zealand ◽

Extraction Method ◽

Acid Precipitation ◽

In Vitro Digestion ◽

Enzymatic Extraction ◽

Physico Chemical ◽

N Release ◽

Food Applications ◽

Small Intestinal

Being widely abundant, grass proteins could be a novel source of plant proteins for human foods. In this study, ryegrass proteins extracted using two different approaches-chemical and enzymatic extraction, were characterised for their physico-chemical and in vitro digestion properties. A New Zealand perennial ryegrass cultivar Trojan was chosen based on its higher protein and lower dry matter contents. Grass protein concentrate (GPC) with protein contents of approximately 55 and 44% were prepared using the chemical and enzymatic approach, respectively. The thermal denaturation temperature of the GPC extracted via acid precipitation and enzymatic treatment was found to be 68.0 ± 0.05 °C and 66.15 ± 0.03 °C, respectively, showing significant differences in protein’s thermal profile according to the method of extraction. The solubility of the GPC was highly variable, depending on the temperature, pH and salt concentration of the dispersion. The solubility of the GPC extracted via enzymatic extraction was significantly lower than the proteins extracted via the chemical method. Digestion of raw GPC was also studied via a gastro-small intestinal in vitro digestion model and was found to be significantly lower, in terms of free amino N release, for the GPC prepared through acid precipitation. These results suggest that the physico-chemical and digestion characteristics of grass proteins are affected by the extraction method employed to extract the proteins. This implies that selection of an appropriate extraction method is of utmost importance for achieving optimum protein functionality during its use for food applications.

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Supplementary feeding of roe deer (Capreolus capreolus L) with late harvested hay. A pilot study

Rangifer ◽

10.7557/2.5.2.540 ◽

1985 ◽

Vol 5 (2) ◽

pp. 6 ◽

Cited By ~ 2

Author(s):

C. Rehbinder ◽

P. Ciszuk

Keyword(s):

Protein Content ◽

Crude Protein ◽

Dry Matter ◽

Roe Deer ◽

Capreolus Capreolus ◽

In Vitro Digestion ◽

Metabolizable Energy ◽

Mild Winter ◽

Normal Behavior

In order to increase the fodder available for roedeer during wintertime, late harvested hay was placed on racks early in November in three consecutive years. Freeezing kept the hay dry and fresh during all three winters. In the first winter, with much snow, the bulk of the hay was consumed whereas consumption in the two subsequent mild winters was low and selective. The crude protein content of the hay was low (3.5 - 8.4% dry matter). Metabolizable energy estimated from digestion in vitro was 3.5 — 5.6 MJ per kg dry matter. Rumen liquor from roe deer during a mild winter gave lower in vitro digestion than liquor from sheep fed with ordinary rations. The value of this poor hay for roe deer is discussed with respect to the animals requirements, seasonal adaption, the energy and protein content of the hay, water consumption and normal behavior. The results indicate that late harvested hay may be more suitable than regularly harvested hay or concentrates to help roe deer to survive spells of severe winter conditions. With late harvested hay placed out at several localized feeding sites, the risks of indigestion and dehydration, associated with a more concentrated, feed, are minimized and the ranking among the roe deer in particular will be less important and thus more animals will have improved prospects of gaining access to the fodder.Tilskottsutfodring av rådjur (Capreolus capreolus L) med sent skordat ho. En pilotstudie.Abstract in Swedish / Sammanfattning: I avsikt att, for rådjur, oka mångden tillgångligt foder under vintertid, skordades och håssjades ho i borjan av november under tre på varandra foljande år. Hoet fros torn och holl sig fårskt alla vintrarna. Forstå vintern med mycket sno konsumerades huvuddelen av hoet medan de två foljande milda vintrarna konsumptionen var låg och selektiv. Mångden råprotein i hoet var lågt (3.5 — 8.4 % i torrsubstans). Innehållet av omsåttbar energi beråknad från digestion in vitro var 3.5 — 5.6 MJ per kg torrsubstans. Våmvåtska från rådjur under en mild vinter gav lagra in vitro digestion ån våmvåtska från ordinårt utfodrade får. Vårdet av det mycket sent skordade hoet for rådjur diskuteras med utgångspunkt från djurens behov, såsongmåssiga adaption, energi- och proteininnehåll i hoet, vattenkonsumtion och normala beteende. Resultatén pekar på att, sent skordat ho kan vara låmpligare ån på normal tid skordat ho eller kraftfoder att hjålpa rådjur att overleva perioder med svåra vinterforhållanden. Med sent skordat och håssjat ho, vilket gjorts tillgångligt på ett flertal utfodringsplatser, minskar riskerna for våmindigestion och dehydrering, forenade med en mer hogvårdig utfodring, samtidigt som djurens inbordes rangordning blir mindre betydelsefull. Det senare innebarande att fler djur får tillgång till fodret.

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An Enteric-Coated Polyelectrolyte Nanocomplex Delivers Insulin in Rat Intestinal Instillations When Combined with a Permeation Enhancer

Pharmaceutics ◽

10.3390/pharmaceutics12030259 ◽

2020 ◽

Vol 12 (3) ◽

pp. 259 ◽

Cited By ~ 3

Author(s):

Svenja Sladek ◽

Fiona McCartney ◽

Mena Eskander ◽

David J. Dunne ◽

Maria Jose Santos-Martinez ◽

...

Keyword(s):

Size Exclusion ◽

Potential Range ◽

Permeation Enhancer ◽

Intestinal Fluid ◽

Oral Peptide Delivery ◽

Free Insulin ◽

Exclusion Chromatography ◽

Small Intestinal ◽

Enteric Coated

The use of nanocarriers is being researched to achieve oral peptide delivery. Insulin-associated anionic polyelectrolyte nanoparticle complexes (PECs) were formed that comprised hyaluronic acid and chitosan in an optimum mass mixing ratio of 5:1 (MR 5), followed by coating with a pH-dependent polymer. Free insulin was separated from PECs by size exclusion chromatography and then measured by HPLC. The association efficiency of insulin in PECs was >95% and the loading was ~83 µg/mg particles. Dynamic light scattering and nanoparticle tracking analysis of PECs revealed low polydispersity, a negative zeta potential range of −40 to −50 mV, and a diameter range of 95–200 nm. Dissolution studies in simulated small intestinal fluid (FaSSIF-V2) revealed that the PECs were colloidally stable. PECs that were coated with Eudragit® L-100 delayed insulin release in FaSSIF-V2 and protected insulin against pancreatin attack more than uncoated PECs. Uncoated anionic PECs interacted weakly with mucin in vitro and were non-cytotoxic to Caco-2 cells. The coated and uncoated PECs, both concentrated further by ultrafiltration, permitted dosing of 50 IU/kg in rat jejunal instillations, but they failed to reduce plasma glucose or deliver insulin to the blood. When ad-mixed with the permeation enhancer (PE), sucrose laurate (100 mM), the physicochemical parameters of coated PECs were relatively unchanged, however blood glucose was reduced by 70%. In conclusion, the use of a PE allowed for the PEC-released bioactive insulin to permeate the jejunum. This has implications for the design of orally delivered particles that can release the payload when formulated with enhancers.

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