scholarly journals The Effect of Dandang Gendis Extract (Clinacanthus nutans) on Kidney Histopathological Features of Diabetic Rats Model

2021 ◽  
Vol 5 (01) ◽  
pp. 19-22
Author(s):  
Miranda Jemyma Mas'ulun ◽  
Arifa Mustika ◽  
Ema Qurnianingsih
Keyword(s):  
Oxidative Stress ◽  
Diabetic Nephropathy ◽  
Tissue Damage ◽  
Diabetic Rats ◽  
Keywords Diabetes ◽  
Standard Drug ◽  
Histopathological Features ◽  
Clinacanthus Nutans ◽  
Minimize Tissue Damage ◽  
Damage Criteria

Changes in kidney homeostasis due to diabetes can cause oxidative stress which then caused tissue damage that leads to diabetic nephropathy. Clinacanthus nutans extract is known to contain antioxidants that are reported to play an important role in the body’s defense system against oxidative stress to minimize tissue damage. This study aims to know the effect of Clinacanthus nutans leaf extract administration on kidney histopathological features of the diabetic rats model. A total of 35 rats were induced by streptozotocin which then divided into 5 groups and given Clinacanthus nutans extract with a dose of 75 mg/kgBW, 150 mg/kgBW and 300 mg/kgBW then compare with CMC-Na as control and metformin as standard drug for 14 days. The kidney histopathology was evaluated under a light microscope against the damage criteria that occurred in the proximal tubules of the kidney. As the result the least amout of kidney damage was on treatment group at dose 300 mg/kgBW, followed by extract with dose 75 mg/kgBW, 150 mg/kgBW, and metformin. In conclusion Clinacanthus nutans extract with a dose of 75 mg/kgBW, 150 mg/kgBW, and 300 mg/kgBW can improve the kidney histopathological feature of the diabetic rats model. Keywords: diabetes mellitus; histopathology; diabetic nephropathy; Clinacanthus nutans

Crocin treatment decreased pancreatic atrophy, LOX-1 and RAGE mRNA expression of pancreas tissue in cholesterol-fed and streptozotocin-induced diabetic rats

2019 ◽  
Vol 17 (2) ◽  
Author(s):  
Mohammad Ehsan Bayatpoor ◽  
Saeed Mirzaee ◽  
Mohammad Karami Abd ◽  
Mohammad Taghi Mohammadi ◽  
Shima Shahyad ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mrna Expression ◽  
Tissue Damage ◽  
Critical Role ◽  
Serum Glucose ◽  
Diabetic Rats ◽  
Control Treatment ◽  
Pcr Analysis ◽  
Control Group ◽  
Cholesterol Levels

AbstractObjectiveOxidative stress in diabetic mellitus is a consequence of oxidative stress, which plays a critical role in the pathogenesis of diabetic tissue damage. Receptors for advanced glycation end products and for oxidized low-density lipoproteins (LDL) have critical contribution in oxidative tissue damage. The present study investigated whether anti-diabetic effects of Crocin via modulation of mRNA expression of RAGE and LOX-1 receptors in diabetic rats.MethodsIn the current study, high-fat cholesterol (HFC) and streptozotocin (40 mg/kg) used to induce type II diabetes. Experimental groups as follows: (Group 1: control); (Group 2: control treatment [Crocin]); (Group 3: DM [STZ]); (Group 4: DM treatment [STZ + Crocin]); (Group 5; DM + HFC [STZ + HFC]); (Group 6; DM + HFC treatment [STZ + HFC + Crocin]). Crocin (20 mg/kg/day, i.p.) administered in treatment groups for 60 days. Serum glucose and cholesterol levels evaluated on days 5, 30 and 60 after induction of DM. Pancreatic tissue from all group removed on day 60 for histological and RT-PCR analysis.ResultsApplication of Crocin significantly decreased serum cholesterol levels on day 60 after induction of DM in diabetic + HFC rats. Moreover, Crocin significantly decreased serum glucose levels on days 30 and 60 both in diabetic and diabetic + HFC rats. Crocin partially prevented the atrophic effects of STZ on both exocrine and endocrine parts of pancreas. Additionally, Crocin significantly decreased LOX-1 and RAGE mRNA expression OF pancreas in diabetic rats.ConclusionThe current study suggested that Crocin suppressed atrophic change of the pancreas by decrease of LOX-1 and RAGE mRNA expression in diabetic rats.

Extract of Moringa concanensis Nimmo leaves ameliorates hyperglycemia and oxidative stress, and improves β-cell function in Alloxan monohydrate induced diabetic rat

2021 ◽  
Vol 17 ◽  
Author(s):  
Amerendra Singh ◽  
Jai Narayan Mishra ◽  
Santosh Kumar Singh ◽  
Vishal Kumar Vishwakarma ◽  
Shravan Kumar Paswan
Keyword(s):  
Oxidative Stress ◽  
Blood Glucose ◽  
Diabetes Management ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Histopathological Analysis ◽  
Β Cells ◽  
Standard Drug ◽  
Alloxan Monohydrate ◽  
And Oxidative Stress

Background: The ethanomedicinal importance of Moringa concanensis Nimmo plant is reflected in Ayurvedic and traditional system of medicine. It brings out its importance as diverse plant in Ayurvedic preparation and diabetes management. Aims of study: The research was centred to bring out the Hyperglycemiccapabilities of Moringa concanensis Nimmo leaves Ethanolic extract (PE) on Alloxan monohydrate (AXM) induceddiabetic rat model. Materials and Methods: Wistar rats were made diabetic by AXM and treated with PE (200 mg/kg body weight) and glibenclamide as a standard drug. All Essential parameters like Fasting blood glucose (FBS), Post prandial blood glucose (PPBS), AST, ALT, ALP, ACP, LDH and oxidative stress markers were measured. Also to see β-cells structures histology of pancreas was also done. Results: The non toxicity of PE dose was confirmed by acute toxicity study and also this study models helped to know about the anti-hyperglycemic effects of PE by decreasing FBS and PPBS levels in the diabetic rats. It also enhances oxidative stress by decreasing MDA levels and elevating the GSH and SOD. The histopathological analysis helped us to know about structure decay of β-cells of pancreas tissue of diabetic rats. PEpotential was confirmed by serum enzymes AST, ALT, ALP, ACP and LDH as it showed significant decrease in diabetic rats. Conclusion: It was confirmed from the data that PE is efficient in governance of diabetes and its control, so there is a need to work at molecular level to bring out all its potential for the benefit of the society.

scholarly journals Apigenin ameliorates streptozotocin-induced diabetic nephropathy in rats via MAPK-NF-κB-TNF-α and TGF-β1-MAPK-fibronectin pathways

2017 ◽  
Vol 313 (2) ◽  
pp. F414-F422 ◽  
Author(s):  
Salma Malik ◽  
Kapil Suchal ◽  
Sana Irfan Khan ◽  
Jagriti Bhatia ◽  
Kamal Kishore ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetic Nephropathy ◽  
Transforming Growth Factor ◽  
Mapk Pathway ◽  
Histopathological Examination ◽  
Fasting Blood Glucose ◽  
Transforming Growth Factor Β1 ◽  
Diabetic Rats ◽  
Tnf Α ◽  
Per Se

Diabetic nephropathy (DN), a microvascular complication of diabetes, has emerged as an important health problem worldwide. There is strong evidence to suggest that oxidative stress, inflammation, and fibrosis play a pivotal role in the progression of DN. Apigenin has been shown to possess antioxidant, anti-inflammatory, antiapoptotic, antifibrotic, as well as antidiabetic properties. Hence, we evaluated whether apigenin halts the development and progression of DN in streptozotocin (STZ)-induced diabetic rats. Male albino Wistar rats were divided into control, diabetic control, and apigenin treatment groups (5–20 mg/kg po, respectively), apigenin per se (20 mg/kg po), and ramipril treatment group (2 mg/kg po). A single injection of STZ (55 mg/kg ip) was administered to all of the groups except control and per se groups to induce type 1 diabetes mellitus. Rats with fasting blood glucose >250 mg/dl were included in the study and randomized to different groups. Thereafter, the protocol was continued for 8 mo in all of the groups. Apigenin (20 mg/kg) treatment attenuated renal dysfunction, oxidative stress, and fibrosis (decreased transforming growth factor-β1, fibronectin, and type IV collagen) in the diabetic rats. It also significantly prevented MAPK activation, which inhibited inflammation (reduced TNF-α, IL-6, and NF-κB expression) and apoptosis (increased expression of Bcl-2 and decreased Bax and caspase-3). Furthermore, histopathological examination demonstrated reduced inflammation, collagen deposition, and glomerulosclerosis in the renal tissue. In addition, all of these changes were comparable with those produced by ramipril. Hence, apigenin ameliorated renal damage due to DN by suppressing oxidative stress and fibrosis and by inhibiting MAPK pathway.

scholarly journals Persea americana Leaf Ethyl Acetate Extract Phytochemical, In-vitro Antioxidant and In-vivo Potentials to Mitigate Oxidative Stress in Alloxan-induced Hyperglycaemic Rats

2019 ◽  
pp. 1-11
Author(s):  
J. A. Mashi ◽  
A. M. Sa’id ◽  
R. I. Idris ◽  
I. Aminu ◽  
A. A. Muhammad ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Diabetic Rats ◽  
Persea Americana ◽  
Albino Rats ◽  
Standard Drug

The purpose of this study was to investigate the in-vivo and in-vitro potentials of ethyl acetate extract of P. americana leaf in alloxan-induced diabetic rats. Quantitative phytochemicals analyzed includes; flavonoids, saponins, tannins, alkaloids and phenolics. Measurement of antioxidant activity using 1,1-Diphenyl-2-picrylhydrazyl, total antioxidant capacity, hydroxyl radical, hydrogen peroxide, superoxide radical and ferric reducing activity of the extract was carried out. Hyperglycemia was induced by intraperitoneal injection of alloxan monohydrate to albino rats. In-vivo anti-oxidant potentials of the extract were evaluated by measuring liver homogenate activity of superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase and malondyaldehyde in alloxan-induced diabetic rats administered with the extract.  A total of 30 Albino rats were used for this experiment and they were divided into six groups of 5 rats each. Group A; normal control, Group B; diabetic control, Groups C-E; experimental groups administered with different doses (100, 200 and 400 mg/kg body weight respectively); of the extract and Group F; glucophage (84 mg/kg body weight, standard drug) for 4 weeks. This study was conducted in the Department of Biochemistry, Bayero University, Kano, in August, 2018. Data was analyzed using one-way ANOVA with P=.05 value considered as significant. Results of the quantitative phytochemical investigation shows that the extract is rich in phenolics (184.1±0.6), flavonoids (115.8±2.1), alkaloids (41.5±1.8), with least concentration of tannis (21.2±0.8) and saponins (15.2±2.3). The extract exhibited high radical scavenging activity against synthetic free radicals (DPPH), reactive oxygen species (peroxide, superoxide and hydroxyl acid) and high ability to reduce Fe3+ to Fe2+ (FRAP). The activities of antioxidant enzymes of the treated rats were increased significantly (P=.05) while the level malondyaldehyde was significantly decreased (P=.05) in the treated groups. Ethyl acetate leaf extract of Persea americana contains phytochemical substances which improved antioxidant status and can be use as herbal therapy for the management of oxidative stress induced by diabetes mellitus and associated complications.

EXPLORATION OF PROTECTIVE EFFECT OF HYDROALCOHOLIC EXTRACT OF ALSTONIA SCHOLARIS BARK IN STZ-INDUCED EARLY DIABETIC NEPHROPATHY MODEL IN RATS

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (08) ◽  
pp. 69-78
Author(s):  
D. D. Bandawane ◽  
S. B Jadhav ◽  
A. R. Juvekar ◽  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Diabetic Nephropathy ◽  
Reduced Glutathione ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Total Proteins ◽  
Hydroalcoholic Extract ◽  
Early Diabetic Nephropathy

Alstonia scholaris (fam. Apocynaceae) is an indigenous plant used traditionally for the treatment of diabetes and associated complications. However the nephroprotective potential of the plant is not scientifically evaluated. Objective of the present was to investigate renal protective activity of hydroalcoholic extract of A. scholaris bark (HEAS) in streptozotocin (STZ)-induced early diabetic nephropathy in rats and to focus on its possible mechanism of action. Experimental diabetes was induced in Wistar rats using single intraperitoneal injection of streptozotocin (65 mg/kg). Animals were divided in five groups (n=6) and treated with 150 mg/kg and 300 mg/kg HEAS for 4 weeks. At the end of study period, fasting blood glucose, blood urea nitrogen (BUN), serum creatinine, total proteins, serum albumin, serum insulin and glycosylated haemoglobin, superoxide dismutase, catalase, reduced glutathione and MDA in kidney were evaluated. Urine was analyzed for albumin, total proteins and creatinine clearance. Kidney and pancreas were subjected for histopathology. Significant decrease in fasting blood glucose, creatinine, albumin, BUN, total proteins and urinary total proteins was observed. Significant improvement in serum insulin, glycosylated Hb, oxidative stress parameters of kidney including superoxide dismutase, catalase and reduced glutathione has been observed in HEAS treated diabetic rats. Histopathology of kidney and pancreatic tissues showed structural improvement. Present study has revealed that HEAS prevented the progression of diabetic nephropathy in STZ-diabetic rats by improving the disturbed glucose homeostasis and by amelioration of renal oxidative stress.

Comparison of the Effects of Korean Ginseng and Heat-Processed Korean Ginseng on Diabetic Oxidative Stress

2008 ◽  
Vol 36 (05) ◽  
pp. 989-1004 ◽  
Author(s):  
Hyun Young Kim ◽  
Ki Sung Kang ◽  
Noriko Yamabe ◽  
Takako Yokozawa
Keyword(s):  
Oxidative Stress ◽  
Diabetic Nephropathy ◽  
Renal Damage ◽  
Advanced Glycation Endproducts ◽  
Diabetic Rats ◽  
Heat Processing ◽  
Diabetic Rat ◽  
Thiobarbituric Acid Reactive Substance ◽  
Pathological Conditions ◽  
Korean Ginseng

To investigate the effects of Korean ginseng (KG, Panax ginseng C.A. Meyer) and heat-processed Korean ginseng (H-KG) on diabetic renal damage, we used the streptozotocin-induced diabetic rat model in this study. The diabetes-induced physiological abnormalities at early-stage were attenuated by KG or H-KG administration through reducing the blood glucose level and improving renal function. The oxidative stress-induced increases in serum and renal thiobarbituric acid-reactive substance levels were significantly reduced by KG and H-KG administrations. Moreover, the protein expressions related to oxidative stress and advanced glycation endproducts were significantly reduced in diabetic rats and/or not significantly increased compared to normal rats by KG or H-KG administration. All of these beneficial effects of H-KG in diabetic rats were stronger than those of KG. Therefore, KG and H-KG may improve diabetic pathological conditions and prevent renal damage associated with diabetic nephropathy, and these protective effects of KG can be improved by heat-processing. This study provides scientific evidence that H-KG may be a potential therapeutic agent for pathological conditions associated with diabetic complications including diabetic nephropathy.

Sign in / Sign up

Export Citation Format

Share Document