scholarly journals Gene Expression Profiling of Early Acute Febrile Stage of Dengue Infection and Its Comparative Analysis With Streptococcus pneumoniae Infection

2021 ◽  
Vol 11 ◽  
Author(s):  
Leena H. Bajrai ◽  
Sayed S. Sohrab ◽  
Thamir A. Alandijany ◽  
Mohammad Mobashir ◽  
Muddassir Reyaz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Streptococcus Pneumoniae ◽  
Infectious Diseases ◽  
Gene Expression Profiling ◽  
Dengue Fever ◽  
Dengue Hemorrhagic Fever ◽  
Expression Profiling ◽  
Antigen Processing ◽  
Dengue Infection ◽  
Hemorrhagic Fever

Infectious diseases are the disorders caused by organisms such as bacteria, viruses, fungi, or parasites. Although many of them are permentantly hazardous, a number of them live in and on our bodies and they are normally harmless or even helpful. Under certain circumstances, some organisms may cause diseases and these infectious diseases may be passed directly from person to person or via intermediate vectors including insects and other animals. Dengue virus and Streptococcus pneumoniae are the critical and common sources of infectious diseases. So, it is critical to understand the gene expression profiling and their inferred functions in comparison to the normal and virus infected conditions. Here, we have analyzed the gene expression profiling for dengue hemorrhagic fever, dengue fever, and normal human dataset. Similar to it, streptococcus pneumoniae infectious data were analyzed and both the outcomes were compared. Our study leads to the conclusion that the dengue hemorrhagic fever arises in result to potential change in the gene expression pattern, and the inferred functions obviously belong to the immune system, but also there are some additional potential pathways which are critical signaling pathways. In the case of pneumoniae infection, 19 pathways were enriched, almost all these pathways are associated with the immune system and 17 of the enriched pathways were common with dengue infection except platelet activation and antigen processing and presentation. In terms of the comparative study between dengue virus and Streptococcus pneumoniae infection, we conclude that cell adhesion molecules (CAMs), MAPK signaling pathway, natural killer cell mediated cytotoxicity, regulation of actin cytoskeleton, and cytokine-cytokine receptor interaction are commonly enriched in all the three cases of dengue infection and Streptococcus pneumoniae infection, focal adhesion was enriched between classical dengue fever — dengue hemorrhagic fever, dengue hemorrhagic fever—normal samples, and SP, and antigen processing and presentation and Leukocyte transendothelial migration were enriched in classical dengue fever —normal samples, dengue hemorrhagic fever—normal samples, and Streptococcus pneumoniae infection.

Comparative Study of Gene Expression Profiling Unravels Functions Associated with Pathogenesis of Dengue Infection

2020 ◽  
Vol 26 (41) ◽  
pp. 5293-5299
Author(s):  
Mohiuddin K. Warsi ◽  
Mohammad A. Kamal ◽  
Mohammed N. Baeshen ◽  
Mohammad A. Izhari ◽  
Ahmad Firoz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Dengue Hemorrhagic Fever ◽  
Expression Profiling ◽  
Dengue Infection ◽  
Gene Expression Pattern ◽  
Dengue Shock Syndrome ◽  
Hemorrhagic Fever ◽  
Critical Pathways ◽  
Temporal Gene Expression

Background: Dengue virus is a potential source of propagating dengue hemorrhagic fever. This virus leads to dengue hemorrhagic fever/dengue shock syndrome, benign syndrome, and severe syndrome and due to its infection, there occurs alterations at multiple levels such as gene expression and pathway levels. So, it is critical to understand the pathogenesis of dengue infection in terms of gene expression and the associated functions. Methods: For this purpose, here, we have analyzed the temporal gene expression profiling for the dengue hemorrhagic fever dataset at 12, 24, and 48 hours. Results: The outcome appears that the dengue hemorrhagic fever evolves differently at different time periods or stages. Counclusions: The change in the gene expression pattern increases exponentially from 12 hours to 48 hours and the number of altered functions (pathways) also increases. Wnt, apoptosis, and transcription signaling are among the critical pathways which are dominantly altered. In the initial phase (first 12 hours), only two pathways are altered due to dengue infection, while in the next 12 hours, eight pathways are altered, and finally, in the next 24 hours, 11 pathways are altered and most of these 11 pathways are very critical in terms of biological pathways and functions.

scholarly journals Gene expression profiling of the response of Streptococcus pneumoniae to penicillin

2007 ◽  
Vol 59 (4) ◽  
pp. 616-626 ◽  
Author(s):  
P. David Rogers ◽  
Teresa T. Liu ◽  
Katherine S. Barker ◽  
George M. Hilliard ◽  
B. Keith English ◽  
...  
Keyword(s):  
Gene Expression ◽  
Streptococcus Pneumoniae ◽  
Gene Expression Profiling ◽  
Expression Profiling

scholarly journals Serum levels of interleukin-6, tumor necrosis factor-alpha and interferon-gama in infants with and without dengue

2008 ◽  
Vol 41 (1) ◽  
pp. 6-10 ◽  
Author(s):  
Berta Nelly Restrepo ◽  
Diana María Isaza ◽  
Clara Lina Salazar ◽  
Ruth Ramírez ◽  
Marta Ospina ◽  
...  
Keyword(s):  
Dengue Fever ◽  
Dengue Hemorrhagic Fever ◽  
Primary Infection ◽  
Secondary Infection ◽  
Dengue Infection ◽  
Hemorrhagic Fever ◽  
Serum Levels ◽  
Tnf Alpha ◽  
Necrosis Factor Alpha ◽  
Ifn Gamma

This study compared the serum levels of IL-6, TNF-alpha and IFN-gamma, in children under 1 year of age with and without dengue. Sera were collected from a total of 41 children living in the Department of Antioquia, Colombia (27 patients with dengue and 14 controls). The results showed higher cytokine levels in children with dengue than without dengue, with statistically significant differences for IL-6 and IFN-gamma. No statistically significant differences were found between clinical forms, although IL-6 and IFN-gamma levels were higher in dengue fever cases than in dengue hemorrhagic fever cases. On the other hand, TNF-alpha levels were higher in dengue hemorrhagic fever than in dengue fever. The levels of IL-6 and TNF-alpha were higher in secondary infection than in primary infection, although IFN-gamma levels were higher in primary infection. These results suggest that IL-6, TNF-alpha and IFN-gamma are involved in dengue infection independently of the clinical form.

scholarly journals Clinical profile of dengue infection at a teaching hospital in North India

2011 ◽  
Vol 6 (07) ◽  
pp. 551-554 ◽  
Author(s):  
Ritu Karoli ◽  
Jalees Fatima ◽  
Zeba Siddiqi ◽  
Khursheed I Kazmi ◽  
Amit R Sultania
Keyword(s):  
Dengue Fever ◽  
Dengue Hemorrhagic Fever ◽  
Indian Subcontinent ◽  
Dengue Infection ◽  
Hemorrhagic Fever ◽  
North India ◽  
World Health ◽  
Health Concern ◽  
Neurological Involvement ◽  
Atypical Manifestations

Introduction: Dengue viral infections are among the most important mosquito-borne diseases of the Indian subcontinent and have become a major global public health concern. Spread of disease has led to increased recognition of atypical manifestations apart from the classical clinical features of dengue infection. Methodology: A cross-sectional study of admitted patients suspected to have dengue infection was conducted during the monsoon and post-monsoon seasons in the year 2010. Patients who had serological confirmation of dengue infection were classified according to World Health Organization definitions of dengue fever and dengue hemorrhagic fever. Clinical and biochemical parameters were compared between the two groups. Results: Out of 356 patients with suspected dengue fever enrolled in the study, 138 (39%) had serologically confirmed dengue infection. Eighty (58%) patients were males and 58 (42%) were females. Ninety-six (70%) patients had classical dengue fever while 42 (30%) had dengue hemorrhagic fever. The most common symptoms were headache (105, 76%), abdominal pain (87, 63%), vomiting (80, 58%), rash (36, 26%), and cutaneous hypersensitivity (22, 16%). Hemorrhagic manifestations were present in 55 (40%) patients. Atypical manifestations were recorded. Notably, 14% of patients had neurological involvement and 4% had acute hepatic failure. Overall mortality was 6% and all fatal cases were due to multi-organ failure. Conclusion: Dengue infection poses a huge burden to the health-care system; its spectrum ranges from mild self-limiting illness to severe fatal disease. It can have varied and multi-systemic manifestations which can go unrecognized. Clinicians should have a high index of suspicion for atypical manifestations.

scholarly journals Dengue fever in the Indian subcontinent: an overview

2010 ◽  
Vol 5 (04) ◽  
pp. 239-247 ◽  
Author(s):  
Ummar Raheel ◽  
Muhammad Faheem ◽  
Mohammad Nasir Riaz ◽  
Naghmana Kanwal ◽  
Farakh Javed ◽  
...  
Keyword(s):  
Sri Lanka ◽  
Infectious Diseases ◽  
Economic Impact ◽  
Dengue Fever ◽  
Dengue Hemorrhagic Fever ◽  
Preventive Measures ◽  
Indian Subcontinent ◽  
Hemorrhagic Fever ◽  
Economic Loss ◽  
Pivotal Role

The Indian Subcontinent has emerged as a scene of many mosquito-borne infectious diseases, including malaria and dengue fever. After the 1990s, the rate of malaria declined owing largely to preventive measures, but at the same time dengue fever (DF) and dengue hemorrhagic fever (DHF) were increasing in the region. Outbreaks were recorded in all countries of the Indian Subcontinent with India, Pakistan, Bangladesh and Sri Lanka on the forefront and suffering from the largest number of cases and deaths. We discuss annual cases of DF/DHF in these four countries and possible factors involved in DF outbreaks. We also discuss prevalent serotypes in this region where data suggest the emergence of DEN2 and DEN3 as the most dominant and lethal serotypes. Climate is an important factor influencing DF outbreaks, and rainfall, temperature and humidity play a pivotal role in DF outbreaks. Finally the economic impact of DF/DHF cases is discussed showing that direct and indirect economic loss due to DF/DHF reaches millions of USD each year.

scholarly journals Importance of Platelet Count and Hematocrit in Dengue Fever in Children

2021 ◽  
Vol 44 (2) ◽  
pp. 74-77
Author(s):  
Farhana Rahat ◽  
Morsheda Khanam ◽  
Kazi Iman ◽  
UK Ghosh ◽  
NK Ghosh
Keyword(s):  
Platelet Count ◽  
Dengue Fever ◽  
Dengue Hemorrhagic Fever ◽  
Dengue Infection ◽  
Tertiary Care ◽  
Dengue Shock Syndrome ◽  
Hemorrhagic Fever ◽  
Warning Signs ◽  
Care Hospital ◽  
Pediatric Age Group

Background: Dengue fever has become one of the most important public health concerns now a day due to increasing complications and fatal outcomes. Dengue hemorrhagic fever and dengue shock syndrome are life threatening but reversible complications of dengue fever. Objectives: This study was performed to evaluate the relationship between platelet count and hematocrit with the severity of dengue infection in pediatric age group. Materials & Methods: This was a prospective observational study which included 280 dengue seropositive children of 1 month to 15 years, conducted during 1st June to 30th November, 2018 in a tertiary care hospital of Dhaka. Results: Out of 280 dengue cases, 187(66.78%) had thrombocytopenia and 88(47%) had raised hematocrit. Among the thrombocytopenic patients 44% had dengue fever, 47% had dengue hemorrhagic fever and 9% dengue shock syndrome. A significant co-relation was observed between the severities of thrombocytopenia and raised hematocrit with the appearance of dengue warning signs in case of dengue hemorrhagic fever. Conclusion: Thrombocytopenia and raised hematocrit were related to the severity of dengue hemorrhagic fever. Bangladesh J Child Health 2020; VOL 44 (2) :74-77

Minority Gene Expression Profiling: Probing the Genetic Signatures of Pathogenesis Using Ribosome Profiling

2020 ◽  
Vol 221 (Supplement_3) ◽  
pp. S341-S357
Author(s):  
Antón Vila-Sanjurjo ◽  
Diana Juarez ◽  
Steev Loyola ◽  
Michael Torres ◽  
Mariana Leguia
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Expression Profiles ◽  
Dengue Infection ◽  
Ribosome Profiling ◽  
Powerful Approach ◽  
Ion Proton ◽  
Disease Related Genes ◽  
Genetic Signatures

Abstract Minority Gene Expression Profiling (MGEP) refers to a scenario where the expression profiles of specific genes of interest are concentrated in a small cellular pool that is embedded within a larger, non-expressive pool. An example of this is the analysis of disease-related genes within sub-populations of blood or biopsied tissues. These systems are characterized by low signal-to-noise ratios that make it difficult, if not impossible, to uncover the desired signatures of pathogenesis in the absence of lengthy, and often problematic, technical manipulations. We have adapted ribosome profiling (RP) workflows from the Illumina to the Ion Proton platform and used them to analyze signatures of pathogenesis in an MGEP model system consisting of human cells eliciting <3% productive dengue infection. We find that RP is powerful enough to identify relevant responses of differentially expressed genes, even in the presence of significant noise. We discuss how to deal with sources of unwanted variation, and propose ways to further improve this powerful approach to the study of pathogenic signatures within MGEP systems.

scholarly journals A clinical case of concurrent mycoplasma pneumonia and dengue hemorrhagic fever in a tourist from Thailand

2022 ◽  
Vol 99 (12) ◽  
pp. 51-56
Author(s):  
N. A. Rozanova ◽  
M. A. Sayfullin ◽  
N. N. Zvereva ◽  
V. E. Larichev ◽  
Ya. E. Grigorieva ◽  
...  
Keyword(s):  
Infectious Diseases ◽  
Dengue Fever ◽  
Dengue Hemorrhagic Fever ◽  
Clinical Case ◽  
Clinical Laboratory ◽  
Hemorrhagic Fever ◽  
Radiological Parameters ◽  
Mycoplasma Pneumonia ◽  
Diagnosis Of Diseases

The article describes a clinical case of concurrent infectious diseases - dengue hemorrhagic fever and mycoplasma pneumonia in a patient who came back from the area endemic for dengue fever. Further, the patient was diagnosed with clostridial colitis which was the complication. Clinical, laboratory, and radiological parameters used in the diagnosis of diseases were evaluated.

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