scholarly journals A Mini Review: The Potential Biomarkers for Non-invasive Diagnosis of Pulpal Inflammation

2021 ◽  
Vol 2 ◽  
Author(s):  
Brahmleen Kaur ◽  
Yoshifumi Kobayashi ◽  
Carla Cugini ◽  
Emi Shimizu
Keyword(s):  
Gingival Crevicular Fluid ◽  
Diagnostic Methods ◽  
Diagnostic Tools ◽  
Non Invasive ◽  
Promising Tool ◽  
Inflammatory Status ◽  
Vital Pulp Therapy ◽  
Pulp Exposure ◽  
Detection Of Biomarkers ◽  
Crevicular Fluid

For assessing the adequacy of vital pulp therapy for an inflamed pulp, the use of non-invasive diagnostic tools is necessary to avoid further damage to the teeth. Detection of biomarkers that are indicative of the inflammatory status in pulp can be a promising tool for this purpose. These biomarkers need to be reliably correlated with pulpal inflammation and to be easily detected without pulp exposure. This mini-review article aims to review biomarkers that are present in gingival crevicular fluid (GCF) in inflamed pulp conditions. Several studies have reported the availability of various biomarkers including cytokines, proteases, elastase, neuropeptides, and growth factors. Non-invasive pulpal diagnostic methods will be useful as well to determine reversibility, irreversibility, or necrosis of inflamed pulp. These types of molecular diagnoses via analyzing the proteome have revolutionized the medical field, and are one of the most promising empirical methodologies that a clinician can utilize for the proactive identification of pulpal disease.

scholarly journals Nitrosative Stress Biomarkers in the Non-Stimulated and Stimulated Saliva, as well as Gingival Crevicular Fluid of Patients with Periodontitis: Review and Clinical Study

Antioxidants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 259 ◽  
Author(s):  
Joanna Toczewska ◽  
Tomasz Konopka ◽  
Anna Zalewska ◽  
Mateusz Maciejczyk
Keyword(s):  
Oral Cavity ◽  
Nitrosative Stress ◽  
Gingival Crevicular Fluid ◽  
Disease Process ◽  
Primary Source ◽  
Diagnostic Methods ◽  
Periodontal Tissues ◽  
Crevicular Fluid ◽  
The Impact ◽  
Gingival Fluid

Diagnosis of periodontopathy is complex and includes defining the cause, type, stage, and grade of periodontitis. Therefore, alternative diagnostic methods are sought to indicate the progression of inflammation or to determine the effectiveness of therapy. Gingival crevicular fluid (GCF) biomarkers can be particularly useful because they most likely reflect the disease process of the periodontal tissues. However, the difficulty of collecting GCF for testing is the reason for the limited use in diagnostics. Because periodontitis is the primary source of nitrogen free radicals in the oral cavity, the aim of the study was to evaluate the biomarkers of nitrosative stress (nitric oxide, peroxynitrite, and S-nitrosothiols) in GCF, non-stimulated and stimulated saliva of 90 patients with periodontitis. The study group was divided into two subgroups, depending on the stage of the disease severity. We showed a significantly higher concentration of all assessed biomarkers in the non-stimulated and stimulated saliva of patients with periodontitis. However, significant changes in GCF has been shown only for peroxynitrite. The studied biomarkers did not correlate with clinical periodontal status, which probably results from their short-duration activity and the impact on a few factors in the oral cavity. Saliva and gingival fluid are not very useful in the differential diagnosis of periodontitis.

Diagnostic Potential of Host Response Mediators

1993 ◽  
Vol 7 (2) ◽  
pp. 175-181 ◽  
Author(s):  
S. Offenbacher ◽  
J.G. Collins ◽  
P.A. Heasman
Keyword(s):  
Disease Activity ◽  
Host Response ◽  
Gingival Crevicular Fluid ◽  
Diagnostic Tools ◽  
Predictive Values ◽  
Diagnostic Measures ◽  
Diagnostic Potential ◽  
Crevicular Fluid ◽  
Sensitivity Specificity ◽  
Considerable Body

This review summarizes the data which relate the validation and application of host response markers as diagnostic tests forperiodontal diseases. Practical considerations regarding the general application and evaluation of a diagnostic test for periodontal disease are presented. Experiments which have documented the performance of host response markers as diagnostic measures of disease activity in terms of sensitivity, specificity, and predictive values are summarized. Particular emphasis is placed on the diagnostic potential of gingival crevicular fluid (GCF) components. The considerable body of evidence supporting the potential application of GCF-PGE2 levels for predicting episodes of disease progression and reflecting disease activity is summarized and placed into perspective for the development of future diagnostic tools.

scholarly journals Bone metabolism and RANKL/RANK/OPG trail in periodontal disease

2016 ◽  
Vol 29 (4) ◽  
pp. 171-175
Author(s):  
Lukasz Czupkallo ◽  
Mansur Rahnama ◽  
Dominik Kielbowicz ◽  
Michal Lobacz ◽  
Maryla Kozicka-Czupkallo
Keyword(s):  
Bone Resorption ◽  
Periodontal Disease ◽  
Defense Mechanisms ◽  
Gingival Crevicular Fluid ◽  
Nf Kappa B ◽  
Periodontal Tissues ◽  
Non Invasive ◽  
Receptor Activator ◽  
Crevicular Fluid ◽  
Necrosis Factor

Abstract Periodontal disease is an inflammatory disease of multifactorial etiology. In order for it to appear there must come to an imbalance between the effects of pathogens and host defense mechanisms. As a result of its course the destruction of structures supporting the teeth appears (periodontium, cement, bone), and consequently leads to teeth loosening and loss. In recent years, the participation of RANKL/RANK/OPG in bone remodeling process was highligted. At the molecular level the bone resorption is regulated through the interaction of the ligand receptor activator of nuclear NF-kappa B (RANKL) and osteoprotegerin (OPG), which is a system of two proteins belonging to the protein tumor necrosis factor (TNF). Recent findings about the RANKL protein and OPG have shed new light on the previously unexplained phenomenon of the basis of bone resorption. Research has shown that both protein OPG and RANKL can be detected in gingival crevicular fluid, which has become a window of opportunity in the analysis of non-invasive markers of periodontal tissues, confirming elevated levels of RANKL protein in periodontal disease, and decreased levels of OPG protein. Bone resorption is initiated by the binding of the RANKL protein to receptors RANK present on the surface of mature osteoclasts, and their precursors, which leads to the differentiation and activation of osteoclasts. OPG, being RANKL’s inhibitor, has, in turn, opposite characteristics to RANKL, resulting in the reduction of osteoclastogenesis process. Despite all this, the exact mechanism of bone resorption has not yet been elucidated.

Current Update on Potential Clinical Applications of Oral Sensors

2020 ◽  
Vol 18 (5) ◽  
pp. 335-350
Author(s):  
Pandurangan Harikrishnan
Keyword(s):  
Orthodontic Treatment ◽  
Gingival Crevicular Fluid ◽  
Clinical Applications ◽  
Oral Microbiome ◽  
Testing Methods ◽  
Medical Field ◽  
Advanced Imaging ◽  
Non Invasive ◽  
Potential Alternative ◽  
Crevicular Fluid

Dentistry is a specialized medical field involving treatment of oro-facial diseases and also in the diagnosis of systemic diseases through oral manifestations. Advanced imaging and sensing tools are emerging for the diagnosis and monitoring of general health, in-particular the non-invasive testing methods are becoming popular. Saliva and gingival crevicular fluid are evolving as potential alternative diagnostic biofluids. Hence, oral biosensors are emerging for applications like detecting changes in the oral microbiome, monitoring caries, periodontal disease, levels of biomolecules, loading forces during orthodontic treatment and temporomandibular joint (TMJ) movement etc. This review discloses the current literature in the applications of oral sensors in oral and systemic diagnostics, monitoring and therapy.

Long-Term Sub-Antimicrobial Doxycycline (Periostat®) as Adjunctive Management in Adult Periodontitis: Effects on Subgingival Bacterial Population Dynamics

1998 ◽  
Vol 12 (1) ◽  
pp. 32-39 ◽  
Author(s):  
J.G. Thomas ◽  
R.J. Metheny ◽  
J.M. Karakiozis ◽  
J.M. Wetzel ◽  
R.J. Crout
Keyword(s):  
Gingival Crevicular Fluid ◽  
Dark Field ◽  
Diagnostic Methods ◽  
Treatment Schedule ◽  
Double Blind ◽  
Oral Flora ◽  
Treatment Groups ◽  
Oral Microbiology ◽  
Double Blind Design ◽  
Crevicular Fluid

Previous trials had indicated that various schedules of sub-antimicrobial doxycycline significantly reduced gingival crevicular fluid (GCF) collagenase activity in adult patients with periodontitis with no evidence of emergent tetracycline-resistant (Tcr) marker oral flora. The purpose of this nine-month study was to expand these observations, emphasizing newer microbial diagnostic methods. Subgingival paper point samples were obtained at baseline (BL), 3, 6, and 9 months. Four subject treatment groups in a double-blind design were evaluated by mechanical scaling and root planing (SRP) and/or 20 mg doxycycline BID (Periostat®). Thirty-eight patients entered the study at baseline (BL). Dark-field microscopy on 260 samples showed that morphotype distribution was independent of treatment schedule. Culture analysis of the 3 most prevalent isolates recovered showed that Streptococcus and Prevotella species accounted for approximately 85% of the 724 cultures. There did not appear to be any overgrowth or replacement by opportunistic oral flora. Of 658 susceptibility patterns evaluated by Etest, the MIC 50/90 and mode MIC showed stable patterns, independent of treatment group. Our findings were different from those of previously published reports, but may be partly explained by the lack of universally standardized methods in oral microbiology and interpretive criteria for susceptibility testing.

scholarly journals A Bioinformatics Systems Biology Analysis of the Current Oral Proteomic Biomarkers and Implications for Diagnosis and Treatment of External Root Resorption

2021 ◽  
Vol 22 (6) ◽  
pp. 3181
Author(s):  
Mahmoud Mona ◽  
Zunnaira Abbasi ◽  
Firas Kobeissy ◽  
Abdulrahman Chahbandar ◽  
Roberta Pileggi
Keyword(s):  
Root Resorption ◽  
Gingival Crevicular Fluid ◽  
Dental Health ◽  
Screening Methods ◽  
Traumatic Injuries ◽  
Non Invasive ◽  
External Root Resorption ◽  
Novel Approach ◽  
Precise Diagnosis ◽  
Crevicular Fluid

External root resorption (ERR) is a silent destructive phenomenon detrimental to dental health. ERR may have multiple etiologies such as infection, inflammation, traumatic injuries, pressure, mechanical stimulations, neoplastic conditions, systemic disorders, or idiopathic causes. Often, if undiagnosed and untreated, ERR can lead to the loss of the tooth or multiple teeth. Traditionally, clinicians have relied on radiographs and cone beam computed tomography (CBCT) images for the diagnosis of ERR; however, these techniques are not often precise or definitive and may require exposure of patients to more ionizing radiation than necessary. To overcome these shortcomings, there is an immense need to develop non-invasive approaches such as biomarker screening methods for rapid and precise diagnosis for ERR. In this review, we performed a literature survey for potential salivary or gingival crevicular fluid (GCF) proteomic biomarkers associated with ERR and analyzed the potential pathways leading to ERR. To the best of our knowledge, this is the first proteomics biomarker survey that connects ERR to body biofluids which represents a novel approach to diagnose and even monitor treatment progress for ERR.

Non-invasive monitoring of gingival crevicular fluid for estimation of blood glucose level

2004 ◽  
Vol 42 (3) ◽  
pp. 322-327 ◽  
Author(s):  
M. Yamaguchi ◽  
Y. Kawabata ◽  
S. Kambe ◽  
K. W⇘rdell ◽  
F. H. Nystrom ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Gingival Crevicular Fluid ◽  
Invasive Monitoring ◽  
Non Invasive ◽  
Crevicular Fluid

Descriptive Evaluation of Antibody Responses to SARS-CoV-2 Infection in Plasma and Gingival Crevicular Fluid in a Nursing Home Cohort—Arkansas, June–August 2020

2021 ◽  
pp. 1-24
Author(s):  
Nicole E. Brown ◽  
Amanda K. Lyons ◽  
Amy J. Schuh ◽  
Megan M. Stumpf ◽  
Jennifer L. Harcourt ◽  
...  
Keyword(s):  
Nursing Home ◽  
Immune Responses ◽  
Neutralizing Antibodies ◽  
Gingival Crevicular Fluid ◽  
Natural Infection ◽  
Nursing Home Residents ◽  
Convenience Sample ◽  
Non Invasive ◽  
Viral Culture ◽  
Crevicular Fluid

Abstract Objective: Characterize and compare SARS-CoV-2–specific immune responses in plasma and gingival crevicular fluid (GCF) from nursing home residents during and after natural infection Design: Prospective cohort Setting: Nursing home Participants: SARS-CoV-2–infected nursing home residents Methods: A convenience sample of 14 SARS-CoV-2–infected nursing home residents, enrolled 4–13 days after real-time reverse transcription polymerase chain reaction diagnosis, were followed for 42 days. Post diagnosis, plasma SARS-CoV-2–specific pan-Immunoglobulin (Ig), IgG, IgA, IgM, and neutralizing antibodies were measured at 5 timepoints and GCF SARS-CoV-2–specific IgG and IgA were measured at 4 timepoints. Results: All participants demonstrated immune responses to SARS-CoV-2 infection. Among 12 phlebotomized participants, plasma was positive for pan-Ig and IgG in all 12, neutralizing antibodies in 11, IgM in 10, and IgA in 9. Among 14 participants with GCF specimens, GCF was positive for IgG in 13 and IgA in 12. Immunoglobulin responses in plasma and GCF had similar kinetics; median times to peak antibody response was similar across specimen types (4 weeks for IgG; 3 weeks for IgA). Participants with pan-Ig, IgG, and IgA detected in plasma and GCF IgG remained positive through this evaluation’s end 46–55 days post-diagnosis. All participants were viral culture negative by the first detection of antibodies. Conclusions: Nursing home residents had detectable SARS-CoV-2 antibodies in plasma and GCF after infection. Kinetics of antibodies detected in GCF mirrored those from plasma. Non-invasive GCF may be useful for detecting and monitoring immunologic responses in populations unable or unwilling to be phlebotomized.

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