scholarly journals Identification of the Carbohydrate and Organic Acid Metabolism Genes Responsible for Brix in Tomato Fruit by Transcriptome and Metabolome Analysis

2021 ◽  
Vol 12 ◽  
Author(s):  
Ning Li ◽  
Juan Wang ◽  
Baike Wang ◽  
Shaoyong Huang ◽  
Jiahui Hu ◽  
...  
Keyword(s):  
Citric Acid ◽  
Organic Acid ◽  
Malic Acid ◽  
High Resolution Mass Spectrometry ◽  
Dominant Role ◽  
Tomato Fruit ◽  
Soluble Solids ◽  
Contributing Factors ◽  
Metabolome Analysis ◽  
Acid Ratio

BackgroundSugar and organic acids not only contribute to the formation of soluble solids (Brix) but also are an essential factor affecting the overall flavor intensity. However, the possible metabolic targets and molecular synthesis mechanisms remain to be further clarified.MethodsUHPLC-HRMS (ultrahigh-performance liquid chromatography and high-resolution mass spectrometry) combined with comparative transcriptome analysis were performed in fruits at green ripe (S1), turning-color (S2), and red ripe (S3) stages of two tomato genotypes TM-1 (Solanum galapagense L., LA0436) and TM-38 (S. lycopersicum L. cultivar M82, LA3475) that vary in fruit Brix.ResultsThe fruit Brix of TM-1 was nearly twice that of TM-38 at S3. Nevertheless, TM-1 accumulated 1.84- and 2.77-fold the L-malic acid and citric acid in red ripe fruit (S3) compared with TM-38, respectively. D-glucose and D-fructose in TM-1 and TM-38 fruits tended to be similar at S3. Concomitantly, the sugar/organic acid ratio of TM-38 fruits were 23. 08-, 4. 38-, and 2.59-fold higher than that of TM-1 fruits at S1, S2, and S3, respectively. Among starch and sucrose (carbohydrate, CHO) metabolism (ko00500) genes, SUS (Solyc07g042550.3) and BAM (Solyc08g077530.3) were positively (r = 0.885–0.931) correlated with the sugar/organic acid ratio. Besides, INV (Solyc09g010080.3 and Solyc09g010090.5.1), AAM (Solyc04g082090.3), 4-α-GTase (Solyc02g020980.2.1), BGL2 (Solyc06g073750.4, Solyc06g073760.3, and Solyc01g081170.3), TPS (Solyc01g005210.2 and Solyc07g006500.3), and TPP (Solyc08g079060.4) were negatively (r = −0.823 to −0.918) correlated with the sugar/organic acid ratio. The organic acid (TCA cycle) metabolism (ko00020) gene ALMT (Solyc01g096140.3) was also negatively (r = −0.905) correlated with the sugar/organic acid ratio.ConclusionCitric acid may play a more dominant role in the sugar/organic acid ratio of the tomato fruit, and the contribution of both L-malic acid and citric acid to the fruit Brix was much greater than that of D-glucose and D-fructose. Genes involved in CHO and TCA metabolism, which have a significant correlation with the sugar/organic acid ratio were considered to be the contributing factors of fruit Brix.

scholarly journals Fruit Physiology and Sugar-Acid Profile of 24 Pomelo (Citrus grandis (L.) Osbeck) Cultivars Grown in Subtropical Region of China

Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2393
Author(s):  
Tengfei Pan ◽  
Muhammad Moaaz Ali ◽  
Jiangmei Gong ◽  
Wenqin She ◽  
Dongming Pan ◽  
...  
Keyword(s):  
Citric Acid ◽  
Organic Acids ◽  
Malic Acid ◽  
High Performance ◽  
Soluble Sugars ◽  
Fruit Weight ◽  
Soluble Solids ◽  
Subtropical Region ◽  
Acid Ratio ◽  
Aconitic Acid

In the present study, the fruit physiology and sugar-acid ratio of 24 pomelo cultivars grown in ten different locations of the subtropical region of China were measured. The contents of soluble sugars and organic acids were quantified using high-performance (HPLC-MS) and ultra-performance liquid chromatography-mass spectrometry (UPLC-MS), respectively. The results revealed that the physiological and basic quality attributes of 24 pomelo cultivars, including fruit weight, fruit width, fruit length, peel thickness, number of segments, pulp weight, pulp color, soluble solids, and Vitamin C, ranged between 264.63–1945.85 g, 8.60–19.56 g, 7.40–20.70 g, 0.46–3.33 mm, 11–18.66, 210.25–1351.66 g, 8.59–15.14 Brix°, and 34.79–84.58 mg/100 g, respectively. Soluble sugars, i.e., fructose, glucose, and sucrose, ranged between 16.25–24.25, 16.17–24.22, and 19.90–55.28 mg/g, respectively. Similarly, Organic acids, i.e., pyruvate, fumaric acids, succinic acid, tartaric acid, quinic acid, citric acid, malic acid, and cis-aconitic acid, in 24 pomelo cultivars ranged between 0.48–1.84, 0.02–0.45, 0–0.05, 0.01–0.1, 0–0.14, 3.01–11.85, 0.18–1.42, and 0.01–0.16 mg/g, respectively. The pomelo cultivars ‘Hongzuanmi’, ‘Minihong’, and ‘Hangwanmi’ exhibited maximum contents of citric acid and pyruvate and showed ultimately excessive organic acids. Overall, the ‘Guanximi’ and its budding cultivars, i.e., ‘Hongroumi’, ‘Huangjinmi’, and ‘Sanhongmi’, had the best quality fruits having maximum sugar-acid ratio. Correlation analysis showed that total soluble sugars had a significantly positive correlation with sucrose contents, while citric acids, malic acid, and pyruvate were positively correlated with total organic acids. The determined sugar-acid profile of pomelo cultivars provides the basis for future elucidation of key mechanisms regulating sugars and acids biosynthesis in pomelo.

Effect of hyperbaric treatments on the quality attributes of tomato

2012 ◽  
Vol 92 (3) ◽  
pp. 541-551 ◽  
Author(s):  
Bernard Goyette ◽  
Clément Vigneault ◽  
Marie Thérèse Charles ◽  
Vijaya G. S. Raghavan
Keyword(s):  
Weight Loss ◽  
Tomato Fruit ◽  
Titratable Acidity ◽  
Quality Attributes ◽  
Soluble Solids ◽  
Postharvest Quality ◽  
Ambient Conditions ◽  
Hyperbaric Treatment ◽  
Acid Ratio ◽  
Significant Difference

Goyette, B., Vigneault, C., Charles, M. T. and Raghavan, V. G. S. 2012. Effect of hyperbaric treatments on the quality attributes of tomato. Can. J. Plant Sci. 92: 541–551. An experimental hyperbaric system was conceptualized, designed and built to explore the effect of hyperbaric treatment on the quality attributes of tomato. Tomato fruits at the early breaker stage were subjected to hyperbaric pressures of 1, 3, 5, 7 or 9 atmabs for different durations (5, 10 or 15 d) at 13°C. The effect of hyperbaric treatment on postharvest quality of tomato fruit was evaluated with an emphasis on weight loss, firmness, color, titratable acidity (TA) and total soluble solids (TSS). Hyperbaric treated tomato fruit ripened more slowly and were characterized by lower respiration rate than fruits kept under ambient conditions. Significant differences in weight loss, color, lycopene concentration and firmness were found between hyperbaric treated and control tomato fruit. No significant difference was found in the sugar/acid ratio (TSS/TA).

scholarly journals Variability of characteristic components of aronia

2014 ◽  
Vol 32 (No. 1) ◽  
pp. 25-30 ◽  
Author(s):  
J. Šnebergrová ◽  
H. Čížková ◽  
E. Neradová ◽  
B. Kapci ◽  
A. Rajchl ◽  
...  
Keyword(s):  
Citric Acid ◽  
Malic Acid ◽  
Titratable Acidity ◽  
Quinic Acid ◽  
Soluble Solids ◽  
Mean Values ◽  
Aronia Melanocarpa ◽  
Black Chokeberry ◽  
The Mean

The variability of characteristic components of aronia (Aronia melanocarpa, black chokeberry) and to evaluate the chemical composition and quality of aronia products in order to complete the already available data and to facilitate authentication of fruit products were estimated. The mean values obtained for the set of aronia fruits were: soluble solids 19.9 °Brix, titratable acidity 9.8 g malic acid/kg, formol number 11.9 ml 0.1M NaOH/100 g, ash 6.7 g/kg, phosphorus 0.34 g/kg, potassium 2.90 g/kg, calcium  0.27 g/kg, magnesium 0.16 g/kg, sucrose 0.10 g/kg, glucose 47.1 g/kg, fructose 37.8 g/kg, sorbitol 66.1 g/kg, malic acid 9.6 g/kg, citric acid 1.0 g/kg, quinic acid 5.0 g/kg, isocitric acid 0.02 g/kg. These values can potentially be a useful tool to determine aronia content in foodstuffs or to determine adulteration of other fruit-based products with aronia. The most promising markers appeared to be the content of sorbitol, quinic acid and characteristic profile of anthocyanins measured by HPLC.  

Composition of Pineapple Juice

1992 ◽  
Vol 75 (2) ◽  
pp. 280-282 ◽  
Author(s):  
Dana A Krueger ◽  
Rae-Gabrielle Krueger ◽  
Jeanne Maciel
Keyword(s):  
Citric Acid ◽  
Carbon Isotope ◽  
Malic Acid ◽  
Isotope Ratio ◽  
Carbon Isotope Ratio ◽  
Soluble Solids ◽  
Minor Constituents ◽  
Pineapple Juice

Abstract Major and some minor constituents were determined for a series of fresh pineapple juices. Results Include: soluble solids 11.2-16.2 g/100 g, acidity (reported as citric acid) 0.46-1.21 g citric acid/100 mL, fructose 1.72-4.75 g/100 mL, glucose 1.21-4.52 g/100 mL, sucrose 2.47-9.73 g/100 mL, citric acid 0.439-1.151 g/100 mL, malic acid 0.073- 0.391, Isocltrlc acid 80-265 mg/L, potassium 830- 1410 mg/L, formol value 0.74-1.69 meq/100 mL, proline 11-44 mg/L, and carbon isotope ratio 13.5- 11.2%o PDB. Use of these compositional values In the detection of adulterated pineapple juice is discussed.

scholarly journals Behavior of Ten Chironja Clones at Three Sites. II. Fruit Quality

1969 ◽  
Vol 64 (3) ◽  
pp. 323-329
Author(s):  
A. Pérez ◽  
E. Boneta ◽  
E. Pérez ◽  
J. Green
Keyword(s):  
Citric Acid ◽  
Fruit Quality ◽  
Acid Content ◽  
Soluble Solids ◽  
Lower Percentage ◽  
Total Soluble Solids ◽  
Acid Ratio ◽  
Citric Acid Content ◽  
Number Of Seeds ◽  
Juice Content

Ten chironja clonal varieties were studied at the Isabela, Corozal, and Adjuntas Substations with respect to their fruit qualities. The total soluble solids at Isabela and Corozal was 11%, significantly lower than that at Adjuntas, (15%). The total soluble solids increased as the tree became older. The citric acid content at the Isabela and Corozal Substations was 0.6%, significantly lower than that at Adjuntas, (0.9%). Acid content varied significantly between years and Substations. The Brix/acid ratio at Adjuntas was 17, significantly lower than that at Isabela and Corozal, where it was 19. The citric acid content varied among the different clonal varieties. The fruits harvested during 1971 were rounder than those of 1972, which were elongated. The fruits at Adjuntas were smaller and had a significantly lower percentage of juice than those at Isabela and Corozal, which were equal in size and juice content. Fruits at Isabela had significantly lower peel percentage than those at Corozal and Adjuntas, which did not differ. Fruits produced at Isabela had significantly more seeds than those at Corozal and Adjuntas. The number of seeds per fruit varied significantly among years and Substations. The 2-3 and 3-6 clonal varieties had 11 and 14 seconds respectively, significantly fewer than those all other clones. Therefore, these two clones should be propagated by growers who prefer chironja fruits with fewer seeds.

scholarly journals CHANGES IN ORGANIC ACIDS AND OTHER COMPOSITIONAL AND PHYSIOLOGICAL ATTRIBUTES OF PEACHES DUE TO CHILLING INJURY.

HortScience ◽  
1992 ◽  
Vol 27 (11) ◽  
pp. 1167c-1167
Author(s):  
T. Wang ◽  
A. R. Gonzalez ◽  
E. E. Gbur ◽  
J. M. Aselage
Keyword(s):  
Citric Acid ◽  
Cold Storage ◽  
Malic Acid ◽  
Storage Time ◽  
Chilling Injury ◽  
Titratable Acidity ◽  
Quinic Acid ◽  
The Other ◽  
Soluble Solids ◽  
Flesh Firmness

Babygold 5 (BG5) and Redhaven (RDH) peaches at maturity 4 were held at 2.3°C for 0, 2 and 4 weeks. After each cold storage treatment half of the fruit sample was evaluated; the other half was ripened for 8 days at 21°C and respiration was measured daily. The evaluations on both samples were for malic, citric and quinic acids, titratable acidity (TA), soluble solids (SS) and flesh firmness. Malic acid in ripened BG5 and RDH Fruits increased relative to their unripened counterpart over the cold storage time; citric acid increased in BG5, decreased in RDH; quinic acid decreased in both cultivars; TA increased; SS decreased in BG5, did not change in RDH; flesh firmness increased in BG5, did not change in RDH. Respiratory rate increased with cold storge time in both cultivars. Overall, BG5 showed more susceptibility to chilling than RDH.

Evaluation of Apple Juice Authenticity by Organic Acid Analysis

1983 ◽  
Vol 66 (6) ◽  
pp. 1517-1520 ◽  
Author(s):  
Randall H Evans ◽  
Anton W Van Soestbergen ◽  
Karen A Ristow
Keyword(s):  
Liquid Chromatography ◽  
Citric Acid ◽  
Organic Acid ◽  
Fumaric Acid ◽  
Malic Acid ◽  
Apple Juice ◽  
Specific Method ◽  
Clear Indication ◽  
Organic Acid Profile ◽  
The Difference

Abstract An organic acid profile provides valuable information regarding authenticity of apple juice. The presence of D-malic acid is a clear indication of adulteration because this isomer does not occur naturally. Fumaric and citric acid levels above trace amounts are also inconsistent with pure apple juice; therefore, measurement of these organic acids may also be used as an authenticity check. Citric acid, total malic acid, and fumaric acid were determined in a single scan by liquid chromatography (LC) for 30 known pure apple juice samples. The L-isomer of malic acid was measured by an enzyme-specific method, and the D-isomer was calculated as the difference between total malic acid and L-malic acid.

scholarly journals Disruption of a putative mitochondrial oxaloacetate shuttle protein in Aspergillus carbonarius results in secretion of malic acid at the expense of citric acid production

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Lei Yang ◽  
Tore Linde ◽  
Abeer H. Hossain ◽  
Mette Lübeck ◽  
Peter J. Punt ◽  
...  
Keyword(s):  
Citric Acid ◽  
Organic Acids ◽  
Organic Acid ◽  
Malic Acid ◽  
Acid Production ◽  
Mitochondrial Membrane ◽  
Citric Acid Production ◽  
Aspergillus Carbonarius ◽  
Transcription Analysis ◽  
Organic Acid Production

Abstract Background In filamentous fungi, transport of organic acids across the mitochondrial membrane is facilitated by active transport via shuttle proteins. These transporters may transfer different organic acids across the membrane while taking others the opposite direction. In Aspergillus niger, accumulation of malate in the cytosol can trigger production of citric acid via the exchange of malate and citrate across the mitochondrial membrane. Several mitochondrial organic acid transporters were recently studied in A. niger showing their effects on organic acid production. Results In this work, we studied another citric acid producing fungus, Aspergillus carbonarius, and identified by genome-mining a putative mitochondrial transporter MtpA, which was not previously studied, that might be involved in production of citric acid. This gene named mtpA encoding a putative oxaloacetate transport protein was expressed constitutively in A. carbonarius based on transcription analysis. To study its role in organic acid production, we disrupted the gene and analyzed its effects on production of citric acid and other organic acids, such as malic acid. In total, 6 transformants with gene mtpA disrupted were obtained and they showed secretion of malic acid at the expense of citric acid production. Conclusion A putative oxaloacetate transporter gene which is potentially involved in organic acid production by A. carbonarius was identified and further investigated on its effects on production of citric acid and malic acid. The mtpA knockout strains obtained produced less citric acid and more malic acid than the wild type, in agreement with our original hypothesis. More extensive studies should be conducted in order to further reveal the mechanism of organic acid transport as mediated by the MtpA transporter.

scholarly journals Total Phenolic Contents, Antioxidant Activities and Organic Acids Composition of Three Selected Fruit Extracts at Different Maturity Stages

2014 ◽  
Vol 2 (2) ◽  
pp. 40-46
Author(s):  
Seri Intan Mokhtar ◽  
Chee Leong Pheen ◽  
Ee Ven Lee ◽  
Nur Ain Abd Aziz
Keyword(s):  
Antioxidant Activity ◽  
Citric Acid ◽  
Organic Acids ◽  
Organic Acid ◽  
Malic Acid ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Young Stage

The total phenolic contents (TPC), antioxidant activities and organic acid composition of Salacca zalacca, Mangifera indica L. Chok Anan and Baccaurea motleyana Hook. F. extracts at different maturity stages were evaluated by using modified Folin-Ciocalteu assay at 765 nm absorbance, 2,2- diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and HPLC RP18 column at 215 nm. TPC content ranged from 63.9 mg GAE/100g to 381.23 mg GAE/100g, whereas the antioxidant activity ranged from 3.10% - 84.45%. The M. indica L. Chok Anan contained malic acid and citric acid in the range of 43.897-73.396 mg/100g FW and 2.681-18.428 mg/100g FW at young to ripe stages, respectively. B. motleyana Hook. F. contained citric acid and tartaric acid in the range of 4.649-6.114 mg/100g FW and 3.645-4.365 mg/100g FW at young to ripe stages, respectively. S. zalacca contained malic acid and citric acid in the range of 4.270-17.926mg/100g FW and 0.845-3.284 mg/100g FW at young to ripe stages, respectively. The highest TPC and antioxidant activity were found in the young stage of S. zalacca fruit extract with lowest inhibitory concentration (IC50) valued at 0.57 mgml-1. There was a positive linear correlation between the TPC and antioxidant activities of the fruit extracts. Malic acid is the predominant organic acid in M. indica L. Chok Anan and S. zalacca, while citric acid is the predominant organic acid in B. motleyana Hook. F. This research suggests that the studied fruits at young stage are a better source of TPC and antioxidants. The tested fruits showed a decrease in the concentration of organic acids with ripening.

scholarly journals Transcriptome Analysis Provides Insights into the Root Response of Chinese Fir to Phosphorus Deficiency

2021 ◽  
Author(s):  
Wanting Chen ◽  
Mengyan Zhou ◽  
Mingzhen Zhao ◽  
Ranhong Chen ◽  
Mulualem Tigabu ◽  
...  
Keyword(s):  
Citric Acid ◽  
Organic Acids ◽  
Organic Acid ◽  
Malic Acid ◽  
Glyoxylate Cycle ◽  
Acid Synthesis ◽  
Chinese Fir ◽  
Purple Acid Phosphatase ◽  
Timber Species ◽  
Pi Deficiency

Abstract Background: Phosphorus is one of the essential elements for plant growth and development, but the content of plant available phosphorus (Pi) in many soil types is low. As a fast-growing timber species, Chinese fir is in great demand of Pi, and the lack of Pi in soil restricts the increase of productivity of Chinese fir plantation. Root morphology and the synthesis and secretion of organic acids play an important role in the uptake of phosphorus, but the molecular mechanisms of Chinese fir in response to Pi deficiency are largely unexplored. Results: In this study, seedlings of Yang 061 clone were grown under three Pi supply levels (0, 5 and 10 mg·L-1 P) and morphological attributes, organic acid content and enzyme activity were measured, the transcriptome data of Chinese fir root system were obtained and the expression levels of phosphorus responsive genes and organic acid synthesis related genes on citric acid and glyoxylate cycle pathway were determined. The results showed that there were 50,808 Unigenes annotated from the transcriptome of Chinese fir roots. Among differentially expressed genes, seven genes of phosphate transporter family and seventeen genes of purple acid phosphatase family were up-regulated by Pi deficiency, two proteins of SPX domain were up-regulated and one was down-regulated. The metabolic pathways of the citric acid and glyoxylate cycle pathway were mapped, and the expression characteristics of the related Unigenes under different phosphorus treatments were analyzed. The genes involved in malic acid and citric acid synthesis were up-regulated, and the activities of the related enzymes were significantly enhanced under long-term stress. The contents of citric acid and malic acid in the roots of Chinese fir increased after 30days of Pi deficiency. Conclusion: The Chinese fir roots showed increased expression of citrate and malate synthesis genes, increased content of organic acids and enhanced activities of related enzymes under Pi deficiency. The results provide a new insight for revealing the molecular mechanism of Pi deficiency and the pathway of organic acid synthesis in Chinese fir roots.

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