scholarly journals Airway Microbiome and Serum Metabolomics Analysis Identify Differential Candidate Biomarkers in Allergic Rhinitis

2022 ◽  
Vol 12 ◽  
Author(s):  
Yuze Yuan ◽  
Chao Wang ◽  
Guoqiang Wang ◽  
Xiaoping Guo ◽  
Shengyu Jiang ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Linoleic Acid ◽  
Acid Metabolism ◽  
Prediction Models ◽  
Inferior Turbinate ◽  
Leukotriene B4 ◽  
Arachidonic Acid Metabolism ◽  
Prostaglandin D2 ◽  
Serum Samples ◽  
Serum Metabolomics

Allergic rhinitis (AR) is a common heterogeneous chronic disease with a high prevalence and a complex pathogenesis influenced by numerous factors, involving a combination of genetic and environmental factors. To gain insight into the pathogenesis of AR and to identity diagnostic biomarkers, we combined systems biology approach to analyze microbiome and serum composition. We collected inferior turbinate swabs and serum samples to study the microbiome and serum metabolome of 28 patients with allergic rhinitis and 15 healthy individuals. We sequenced the V3 and V4 regions of the 16S rDNA gene from the upper respiratory samples. Metabolomics was used to examine serum samples. Finally, we combined differential microbiota and differential metabolites to find potential biomarkers. We found no significant differences in diversity between the disease and control groups, but changes in the structure of the microbiota. Compared to the HC group, the AR group showed a significantly higher abundance of 1 phylum (Actinobacteria) and 7 genera (Klebsiella, Prevotella and Staphylococcus, etc.) and a significantly lower abundance of 1 genus (Pelomonas). Serum metabolomics revealed 26 different metabolites (Prostaglandin D2, 20-Hydroxy-leukotriene B4 and Linoleic acid, etc.) and 16 disrupted metabolic pathways (Linoleic acid metabolism, Arachidonic acid metabolism and Tryptophan metabolism, etc.). The combined respiratory microbiome and serum metabolomics datasets showed a degree of correlation reflecting the influence of the microbiome on metabolic activity. Our results show that microbiome and metabolomics analyses provide important candidate biomarkers, and in particular, differential genera in the microbiome have also been validated by random forest prediction models. Differential microbes and differential metabolites have the potential to be used as biomarkers for the diagnosis of allergic rhinitis.

Serum metabolomics reveals dysregulation and diagnostic potential of oxylipins in tumor-induced osteomalacia

2021 ◽  
Author(s):  
Yiyi Gong ◽  
Xiaolin Ni ◽  
Chenxi Jin ◽  
Xiang Li ◽  
Yujie Wang ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Fibroblast Growth Factor 23 ◽  
Tumor Resection ◽  
High Sensitivity ◽  
Leukotriene B4 ◽  
Arachidonic Acid Metabolism ◽  
Serum Samples ◽  
Diagnostic Potential ◽  
Liquid Chromatography Tandem Mass ◽  
Diagnostic Panel

Abstract Context Excessive production of fibroblast growth factor 23 (FGF23) by tumor was considered as the main pathogenesis in tumor-induced osteomalacia (TIO). Despite its importance to comprehensive understanding of pathogenesis and diagnosis, the regulation of systemic metabolism in TIO remains unclear. Objectives We aimed to systematically characterize the metabolome alteration associated with TIO. Methods By means of liquid chromatography-tandem mass spectrometry (LC-MS) based metabolomics, we analyzed the metabolic profile from 96 serum samples (32 initial diagnosis TIO patients, pairwise samples after tumor resection and 32 matched healthy control subjects). In order to screen and evaluate potential biomarkers, statistical analyses, pathway enrichment and receiver operating characteristic (ROC) were performed. Results Metabolomic profiling revealed distinct alterations between TIO and HC cohort. Differential metabolites were screened and conducted to functional clustering and annotation. Significantly enriched pathway was found involved in arachidonic acid metabolism. A combination of 5 oxylipins, 4-HDoHE, leukotriene B4, 5-HETE, 17-HETE and 9,10,13-TriHOME, demonstrated a high sensitivity and specificity panel for TIO prediction screened by random forest (RF) algorithm (AUC=0.951, 95% confidence interval, CI 0.827-1). Supported vector machine (SVM) model and partial least-squares (PLS) model were conducted to validate the predictive capabilities of the diagnostic panel. Conclusions Metabolite profiling of TIO altered significant compared with HC. A high sensitivity and specificity panel with 5 oxylipins were tested as diagnostic predictor. For the first time, we provide the global profile of metabolomes and identify potential diagnostic biomarkers of TIO. The present work may offer novel insights into the pathogenesis of TIO.

scholarly journals Lomatogonium Rotatum for Treatment of Acute Liver Injury in Mice: A Metabolomics Study

Metabolites ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 227 ◽  
Author(s):  
Renhao Chen ◽  
Qi Wang ◽  
Lanjun Zhao ◽  
Shilin Yang ◽  
Zhifeng Li ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Linoleic Acid ◽  
Liver Injury ◽  
Acid Metabolism ◽  
Acute Liver Injury ◽  
Tca Cycle ◽  
Arachidonic Acid Metabolism ◽  
Hepatoprotective Effect ◽  
Metabolomics Study ◽  
Metabolomics Analysis

Lomatogonium rotatum (L.) Fries ex Nym (LR) is used as a traditional Mongolian medicine to treat liver and bile diseases. This study aimed to investigate the hepatoprotective effect of LR on mice with CCl4-induced acute liver injury through conventional assays and metabolomics analysis. This study consisted of male mice (n = 23) in four groups (i.e., control, model, positive control, and LR). The extract of whole plant of LR was used to treat mice in the LR group. Biochemical and histological assays (i.e., serum levels of alanine transaminase (ALT) and aspartate transaminase (AST), and histological changes of liver tissue) were used to evaluate LR efficacy, and metabolomics analysis based on GC-MS and LC-MS was conducted to reveal metabolic changes. The conventional analysis and metabolomic profiles both suggested that LR treatment could protect mice against CCl4-induced acute liver injury. The affected metabolic pathways included linoleic acid metabolism, α-linolenic acid metabolism, arachidonic acid metabolism, CoA biosynthesis, glycerophospholipid metabolism, the TCA cycle, and purine metabolism. This study identified eight metabolites, including phosphopantothenic acid, succinic acid, AMP, choline, glycerol 3-phosphate, linoleic acid, arachidonic acid, and DHA, as potential biomarkers for evaluating hepatoprotective effect of LR. This metabolomics study may shed light on possible mechanisms of hepatoprotective effect of LR.

scholarly journals Dietary Linoleic Acid Lowering Reduces Lipopolysaccharide-Induced Increase in Brain Arachidonic Acid Metabolism

2016 ◽  
Vol 54 (6) ◽  
pp. 4303-4315 ◽  
Author(s):  
Ameer Y. Taha ◽  
Helene C. Blanchard ◽  
Yewon Cheon ◽  
Epolia Ramadan ◽  
Mei Chen ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Linoleic Acid ◽  
Acid Metabolism ◽  
Arachidonic Acid Metabolism ◽  
Dietary Linoleic Acid

Serum metabolomics identifies citrulline as a predictor of adverse outcomes in an equine model of gut-derived sepsis

2014 ◽  
Vol 46 (10) ◽  
pp. 339-347 ◽  
Author(s):  
Samantha M. Steelman ◽  
Philip Johnson ◽  
Amy Jackson ◽  
James Schulze ◽  
Bhanu P. Chowdhary
Keyword(s):  
Critically Ill ◽  
Acid Metabolism ◽  
Gastrointestinal Disease ◽  
Weight Bearing ◽  
Multiple Organ ◽  
Adverse Outcomes ◽  
Serum Samples ◽  
Plasma Citrulline ◽  
Normal Ranges ◽  
Serum Metabolomics

Acute laminitis is an inflammatory disease of the equine foot that often occurs secondarily to sepsis or systemic inflammation associated with gastrointestinal disease. It has been suggested that laminitis is similar to multiple organ dysfunction syndrome in humans, although in horses the weight-bearing laminar epithelium of the foot appears to be the tissue most sensitive to insult and the first “organ” to fail. Metabolomics performed on serum samples collected before (Con) and after (Lmn) experimental induction of gastrointestinal-associated sepsis in six horses detected 1,177 metabolites of both mammalian and bacterial origin in equine serum. Network and correlation analyses suggested a dysregulation of fatty acid metabolism in the Lmn group, as well as an accumulation of organic acids such as lactate. Furthermore, concentrations of the amino acid citrulline were decreased in Lmn samples from all study animals, suggesting that citrulline might be useful as a biomarker to identify critically ill animals that are at risk of developing laminitis. We therefore established normal ranges of plasma citrulline concentrations in a separate group of horses ( n = 36) and tested the ability of citrulline to predict adverse outcomes (laminitis or death) in critically ill horses ( n = 23). Plasma citrulline was significantly lower in critically ill horses that went on to experience adverse outcomes ( n = 6). Further study is required to accurately determine a diagnostic cutoff, but the present data are suggestive of the predictive value of citrulline as a biomarker for laminar failure in equine sepsis.

scholarly journals Serum Metabolomics Analysis for Biomarkers of Lactobacillus plantarum FRT4 in High-Fat Diet-Induced Obese Mice

Foods ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 184
Author(s):  
Hongying Cai ◽  
Zhiguo Wen ◽  
Xin Xu ◽  
Jiaxin Wang ◽  
Xuan Li ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
High Fat Diet ◽  
Ultra Performance Liquid Chromatography ◽  
Leukotriene B4 ◽  
Arachidonic Acid Metabolism ◽  
High Fat ◽  
Flight Mass Spectrometry ◽  
Obesity In Mice ◽  
Gut Microbiota Dysbiosis ◽  
Serum Metabolomics

Lactobacillus plantarum is considered a potential probiotic supplementation for treating obesity. However, the underlying molecular mechanism is poorly understood. Our previous study displayed that L. plantarum FRT4 alleviated obesity in mice fed a high-fat diet (HFD) through ameliorating the HFD-induced gut microbiota dysbiosis. To explore the roles of FRT4 in obesity prevention, in this study, we investigated changes in serum metabolomic phenotype by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS) and analyzed the pathway of HFD-fed Kunming female mice orally administered with FRT4 for eight weeks. Using orthogonal partial least squares discriminant analysis (OPLS-DA), metabolite patterns with significant changes were observed. 55 metabolites including phosphatidylcholine, lysophophatidylcholine, sphingomyelin, serotonin, indole-3-methyl aceta, indole-3-carbinol, indole-5,6-quino, 11,12-DHET, prostaglandin B2, leukotriene B4, and 3-hydroxybenzoic acid were identified as potential biomarkers associated with obesity, which were mainly involving in glycerophospholipid metabolism, tryptophan metabolism, and arachidonic acid metabolism. Perturbations of 14 biomarkers could be regulated by FRT4 intervention. These metabolites may serve as valuable biomarkers to understand the mechanisms by which intake of diets containing FRT4 contributes to the treatment or prevention of obesity. Thus, FRT4 can be a promising dietary supplement for the prevention of HFD-induced obesity.

Prostaglandin D2 is the major prostaglandin of arachidonic acid metabolism in rat bone marrow homogenate

1980 ◽  
Vol 20 (1) ◽  
pp. 171-176 ◽  
Author(s):  
Atsuko Kojima ◽  
Masataka Shiraki ◽  
Ryutaro Takahashi ◽  
Hajime Orimo ◽  
Ikuo Morita ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Arachidonic Acid ◽  
Acid Metabolism ◽  
Arachidonic Acid Metabolism ◽  
Prostaglandin D2 ◽  
Rat Bone

scholarly journals 2-Deoxy-D-glucose Alleviates Collagen-Induced Arthritis of Rats and Is Accompanied by Metabolic Regulation of the Spleen and Liver

2021 ◽  
Vol 12 ◽  
Author(s):  
Hongxing Wang ◽  
Nanyang Zhang ◽  
Kehua Fang ◽  
Xiaotian Chang
Keyword(s):  
Metabolic Pathways ◽  
Metabolic Regulation ◽  
Acid Metabolism ◽  
Regulatory Mechanism ◽  
Leukotriene B4 ◽  
Bile Secretion ◽  
Arachidonic Acid Metabolism ◽  
Biosynthesis Pathway ◽  
Collagen Induced Arthritis ◽  
Linoleic Acid Metabolism

Rheumatoid arthritis (RA) is significantly associated with glycolysis. This study used 2-deoxy-D-glucose (2-DG), an inhibitor of glycolysis, to treat rats with collagen-induced arthritis (CIA) and investigate the metabolic regulatory mechanism of glycolysis in the disease. 2-DG significantly alleviated CIA. Metabolomics and transcriptomics, as well as their integrative analysis, detected significant changes in the pathways of bile secretion, cholesterol and linoleic acid metabolism in the plasma, liver and spleen during the CIA process and the opposite changes following 2-DG treatment, whereas the expression of the genes regulating these metabolic pathways were changed only in the spleen. In the rat liver, levels of (S)-5-diphosphomevalonic acid in the terpenoid backbone biosynthesis pathway were significantly decreased during CIA progression and increased following 2-DG treatment, and levels of taurochenodeoxycholic acid in the pentose and glucuronate interconversions pathway showed the opposite results. In the spleen, levels of 3-methoxy-4-hydroxyphenylglycol glucuronide in bile secretion and 12(S)-leukotriene B4 in arachidonic acid metabolism were significantly decreased during CIA progression and increased following 2-DG treatment. The changes in the gene-metabolite network of bile secretion in the spleen correlated with a decreased plasma L-acetylcarnitine level in CIA rats and an increase following 2-DG treatment. Our analysis suggests the involvement of spleen and liver metabolism in CIA under the control of glycolysis.

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