Development of an Innovative Process for High-Temperature Fruit Juice Extraction Using a Novel Thermophilic Endo-Polygalacturonase From Penicillium oxalicum

Bacillus subtilis is a spore-forming bacterium and an important food contaminant. The aim of this study was to analyze the ability of B. subtilis spores to survive under conditions of low pH and high temperature. The package was purchased at a local supermarket, in Uberaba, Minas Gerais. A sample was collected, diluted and plated on Brain-Heart-Infusion agar (BHI). After incubation, suspected colonies of B. subtilis were transferred to BHI agar. Cell morphology, the presence of spores and Gram stain were examined, and the isolate was identified by 16S rRNA gene sequencing . The microscope evaluation indicated the presence of spores. The thermal tolerance of the spores was evaluated by the addition of 3x109spores/mL in test tubes containing peptone water. Heat treatments were carried out at 80 and 90°C at different incubation times (0, 10, 20, 30, 40, 50 and 60 min). After heating, the tubes were cooled and the number of viable spores was determined in BHI Agar. For the analysis of spore survival, D and Z values were calculated. Tolerance to acid conditions was evaluated using BHI broth with different pH values. After incubation, the bacterial concentration was determined by determining viable cell count on BHI Agar medium. The vegetative cells were transferred to the BHI broth and the pH was adjusted to different values (3, 4 or 5). Sampling were taken 8, 12 and 24 h after incubation. The samples were serially diluted in peptone water and spread in BHI Agar to determine the viable cell count . The 16S rRNA gene sequencing indicated high similarity (99.99%) with B. subtilis. D values were 17.01 min at 80°C and 13.42 min at 90°C. The Z-value was 97.13°C. B. subtilis was not able to grow at pH 3 and pH 4, but its survival was confirmed after the growth of colonies on BHI agar. At pH 5, B. subtilis grew after 24 h and the final pH changed to 7. Our results suggest that the spores of B. subtilis isolated from fruit juice-added soy beverage are tolerant to low pH and high temperature.

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Use of Fruit Juice in the Diets of Young Children

PEDIATRICS ◽

10.1542/peds.95.3.433 ◽

1995 ◽

Vol 95 (3) ◽

pp. 433-433

Author(s):

William J. Klish

Keyword(s):

United States ◽

Young Children ◽

Fruit Juice ◽

The United States ◽

Information Resources ◽

Adequate Supply ◽

The Past ◽

Juice Extraction ◽

Excessive Consumption

Fruit juice is a modern addition to the infant's diet due to the development of technology for juice extraction and an adequate supply of fruit. It has been marketed as healthy, natural, and nutritious. As a result, parents (in trying to do what is best for their infants) have been feeding juices in increasing quantities. In 1992, $164 million was spent on bottled baby juice in the United States according to Information Resources, Inc, a marketing company that tracks the consumer industry. For the past several decades, pediatricians have recognized that excessive consumption of fruit juice has been associated with diarrhea, gassiness, stomachaches, and other abdominal complaints.

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Economic efficiency and investment potential in the smallholder crop sector in Malawi

International Journal of Social Economics ◽

10.1108/03068290010336973 ◽

2000 ◽

Vol 27 (7/8/9/10) ◽

pp. 968-979 ◽

Cited By ~ 2

Author(s):

Julius H. Mangisoni

Keyword(s):

Crop Production ◽

Net Present Value ◽

Fruit Juice ◽

Financial Analysis ◽

Cost Ratio ◽

Full Potential ◽

Extraction Plant ◽

Benefit Cost Ratio ◽

Juice Extraction ◽

Benefit Cost

The crop sector in Malawi faces a number of constraints which must be overcome before agriculture can reach its full potential. This paper uses the policy analysis matrix (PAM) to assess efficiency in the crop sector and financial analysis to explore the potential of investing in a fruit juice extraction plant in Malawi. The PAM revealed that farmers in Malawi are efficient producers but they face negative incentives in the production and marketing of their products. The nominal protection coefficients (NPCs) for both maize and beans were less than 1 while those of inputs were more than 1. Similarly, the effective protection coefficients (EPCs) were less than 1, implying that the combined effect of transfers and tradable inputs is reducing the private profitability of the systems. On the other hand, the financial analysis demonstrated that Malawi can sustain a fruit juice extraction plant. The project had a positive net present value and a benefit/cost ratio greater than 1 (1.16). Recommendations made from the study relate to the need for diversification into fruits that can sustain a fruit juice extraction plant, encouraging private sector participation and reducing disincentives to crop production.

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Purification, characterization of pectinase produced from apple pomace and its evaluation in the fruit juice extraction and clarification

Journal of Biotechnology ◽

10.1016/j.jbiotec.2008.07.1866 ◽

2008 ◽

Vol 136 ◽

pp. S294

Author(s):

V.K. josh ◽

Mukesh Parmar ◽

Neerja Rana

Keyword(s):

Fruit Juice ◽

Apple Pomace ◽

Juice Extraction

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Solvothermal Modified Layers against High Temperature Corrosion

Materials Science Forum ◽

10.4028/www.scientific.net/msf.825-826.621 ◽

2015 ◽

Vol 825-826 ◽

pp. 621-627 ◽

Cited By ~ 2

Author(s):

Silke Wöllmer ◽

Andrea Förg ◽

Sebastian Schuster ◽

Patrick J. Masset

Keyword(s):

High Temperature ◽

Driving Force ◽

State Of The Art ◽

Waste Incineration ◽

High Temperature Corrosion ◽

Self Healing ◽

Waste Gas ◽

Innovative Process ◽

Lower Porosity ◽

Nickel Based Alloy

Service and downtimes of waste incineration plants generate high expenses for their operators. State of the art is to protect high corrosion exposed components by cladding them with a nickel based alloy. The welded overlay is 2-3 mm thick and very expensive. It needs to be repaired at each service interval. An alternative coating consists of a thermal sprayed multilayer of a nickel based bondcoat and a ceramic topcoat. Thickness and costs of this multilayer are assumed to be in the range of about 10% of the currently used coating. A solvothermal treatment chemically densifies the coating. This leads to a significantly lower porosity whilst improving cohesion and hardness properties. This innovative process leads to a self-healing layer caused by the high temperature waste gas exposition as the driving force.

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Fruit juice extraction using a two-stage decanter system

Filtration + Separation ◽

10.1016/s0015-1882(97)84044-x ◽

1995 ◽

Vol 32 (3) ◽

pp. 212 ◽

Cited By ~ 1

Keyword(s):

Fruit Juice ◽

Two Stage ◽

Juice Extraction

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Screening and Molecular Identification of Novel Pectinolytic Bacteria from Forest Soil

Fermentation ◽

10.3390/fermentation7010040 ◽

2021 ◽

Vol 7 (1) ◽

pp. 40

Author(s):

Sarita Shrestha ◽

Janak Raj Khatiwada ◽

Xiaodong Zhang ◽

Chonlong Chio ◽

Aristide Laurel Mokale Kognou ◽

...

Keyword(s):

Forest Soil ◽

Fruit Juice ◽

Xylanase Activity ◽

Plant Fiber ◽

Sesame Seeds ◽

Rdna Sequencing ◽

Juice Extraction ◽

Pectinase Activity ◽

Industrial Demand ◽

Fiber Processing

Pectinases are a group of enzymes with broad application, including in plant fiber processing, pectic wastewater treatment, paper pulping, fruit juice extraction, and clarification. With an increasing industrial demand for these enzymes, it is useful to isolate organisms that produce large amounts of pectinase and possess wide ranges of stability factors like temperature and pH. In this study, 17 out of 29 bacteria (58.62%) from forest soil samples were pectinolytic. However, only four bacteria (S-5, S-10, S-14, and S-17) showed high pectin hydrolysis zones (ranging from 0.2 cm to 1.7 cm). These four bacteria were identified based on colony morphology, microscopic characterization, biochemical characteristics, and 16S rDNA sequencing. They were designated as Streptomyces sp. (S-5, S-14), Cellulomonas sp. (S-10), and Bacillus sp. (S-17). Interestingly, bacteria showed cellulase and xylanase activity in addition to pectinase. The quantitative assay for pectinase activity of the four isolates provided proof that they are pectinase producers and can be considered potential candidates for industrial uses. The crude enzyme extracts of these bacteria are applicable in oil and juice extraction from sesame seeds and apples, respectively.

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