scholarly journals Pathogenomics Characterization of an Emerging Fungal Pathogen, Fusarium oxysporum f. sp. lycopersici in Greenhouse Tomato Production Systems

2020 ◽  
Vol 11 ◽  
Author(s):  
Tika B. Adhikari ◽  
Anne Gao ◽  
Thomas Ingram ◽  
Frank J. Louws
Pathogens ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 70 ◽  
Author(s):  
Sandra L. Carmona ◽  
Diana Burbano-David ◽  
Magda R. Gómez ◽  
Walter Lopez ◽  
Nelson Ceballos ◽  
...  

In Colombia, tomato production under protected conditions represents an important economic contribution to the agricultural sector. Fusarium wilt diseases, caused by pathogenic formae speciales of the soil-borne fungus Fusarium oxysporum Schltdl., cause significant yield losses in tomatoes throughout the world. Investigation of the F. oxysporum–tomato pathosystem in Colombia is required to develop appropriate alternative disease management. In this study, 120 fungal isolates were obtained from four different departments in the Central Andean Region in Colombia from tomato crops with symptoms of wilt disease. A molecular characterization of the fungal isolates was performed using the SIX1, SIX3, and SIX4 effector genes of Fusarium oxysporum f. sp. lycopersici W.C. Snyder & H.N. Hansen (Fol). Additionally, we developed a new specific marker to distinguish between Fusarium oxysporum f. sp. radicis-lycopersici Jarvis & Shoemaker (Forl) and Fol isolates. Furthermore, a phylogenetic analysis using the Translation Elongation Factor 1-alpha (EF1a) gene was performed with the collected isolates. Two isolates (named Fol59 and Fol-UDC10) were identified as Fol race 2, four isolates were identified as Forl, six isolates were identified as F. solani, and most of the isolates were grouped within the F. oxysporum species complex. The phylogenetic tree of EF1a showed that most of the isolates could potentially correspond to nonpathogenic strains of F. oxysporum. Additional pathogenicity assays carried out with Fol59 and Fol-UDC10 confirmed that both isolates were highly virulent strains. This study represents a contribution to the understanding of the local interaction between tomatoes and F. oxysporum in Colombia.


2015 ◽  
pp. 275-280 ◽  
Author(s):  
F. Baysal-Gurel ◽  
C.J. Kurowski ◽  
R. Li ◽  
K.-S. Ling ◽  
S.A. Miller

HortScience ◽  
2004 ◽  
Vol 39 (7) ◽  
pp. 1650-1651 ◽  
Author(s):  
Logan S. Logendra ◽  
Jonathan G. Mun ◽  
Thomas J. Gianfagna ◽  
Harry W. Janes

Ethephon (2-chloroethylphosphonic acid) was applied to single cluster greenhouse tomato crops (1000 ppm) at the green mature stage of fruit development or when 35% of the plants had fruits at the breaker stage. Fruits were harvested at the pink stage. Untreated fruit were harvested from 95 to 116 days after sowing whereas fruit from the green mature ethephon treatment were harvested from 92 to 102 days, three days earlier and with a reduction in the harvest window from 22 to 11 days. Fruit treated with ethephon at 35% breaker were harvested at the same time as untreated fruit, but harvest was completed after only 12 days. Fruit yield from the green mature ethephon treatment was reduced by about 30%, but there was no significant difference in fruit yield as a result of ethephon treatment at 35% breaker. Fruit color, firmness and soluble solids were evaluated one and six days after harvest. Fruit firmness and soluble solids were unaffected by treatment; however, fruit from the ethephon treatments were significantly redder in color. In a second experiment, ethephon was applied at 500 or 1000 ppm when 100% of the plants had fruit at the breaker stage. Fruit were harvested over a 7-day time interval compared to untreated fruit that were harvested over 14 days, and there was a small but significant increase in fruit yield for the 1000 ppm treatment. Both ethephon treatments also increased fruit soluble solids. For limited cluster tomato production systems, ethephon is effective in reducing the harvest window without loss in postharvest fruit quality.


2011 ◽  
Vol 3 (4) ◽  
pp. 58-65 ◽  
Author(s):  
Parviz REZVANI MOGHADDAM ◽  
Hassan FEIZI ◽  
Farzad MONDANI

Efficient use of energy helps to achieve improved production and productivity, and contributes to economy, profitability and competitiveness of agricultural sustainability. The aim of the present study was to compare open field and greenhouse tomato production systems in terms of energy efficiency, energy intensiveness, energy productivity, benefit to cost ratio and amount of renewable and non-renewable energy uses. Data were collected from 128 and 16 open field and greenhouse tomato growers, respectively, by using a face-to-face questionnaire in 2010. The results showed that the total energy requirement under open field and greenhouse systems were 47647.12 and 2102678.73 MJ ha-1, respectively. The share of direct, indirect, renewable and non-renewable energies from total energy input which average in open field and greenhouse production systems were 74%, 26%, 17% and 83%, respectively. Energy use efficiency was achieved 1.42 and 0.18 in open field and greenhouse, respectively. The benefit to cost ratios of 2.33 in open field and 3.06 in greenhouse was recorded. Based on the present results, open field tomato production system had higher energy efficiency in comparison with greenhouse tomato production system while greenhouse system had a higher economical benefit.


2020 ◽  
Vol 13 (11) ◽  
pp. 1
Author(s):  
A. R. B. Zanco ◽  
A. Ferreira ◽  
G. C. M. Berber ◽  
E. N. Gonzaga ◽  
D. C. C. Sabino

The different integrated production systems can directly interfere with its bacterial community. The present study aimed to assess density, bacterial diversity and the influence of dry and rainy season in different integrated and an exclusive production system. The fallow and a native forest area was assessed to. Samples were collected in 2012 March and September. The isolation were carried out into Petri dishes containing DYGS medium. The number of colony forming units (CFU) was counted after 48 hours and. The bacterial density ranged between 106 and 107 CFU g-1 soil. The crop system affected the dynamics of the bacterial community only in the rainy season. The rainy season showed greater density of total bacteria when compared to the dry period regardless of the cropping system. The dendrograms with 80 % similarity showed thirteen and fourteen groups in the rainy and dry seasons. Isolates with the capacity to solubilize phosphate in vitro were obtained from all areas in the two seasons, but this feature has been prevalent in bacteria isolated during the rainy season


2002 ◽  
Vol 51 (5) ◽  
pp. 605-611 ◽  
Author(s):  
F. M. Alves-Santos ◽  
L. Cordeiro-Rodrigues ◽  
J. M. Sayagués ◽  
R. Martín-Domínguez ◽  
P. García-Benavides ◽  
...  
Keyword(s):  

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1152
Author(s):  
Rebekah Waller ◽  
Murat Kacira ◽  
Esther Magadley ◽  
Meir Teitel ◽  
Ibrahim Yehia

Recognizing the growing interest in the application of organic photovoltaics (OPVs) with greenhouse crop production systems, in this study we used flexible, roll-to-roll printed, semi-transparent OPV arrays as a roof shade for a greenhouse hydroponic tomato production system during a spring and summer production season in the arid southwestern U.S. The wavelength-selective OPV arrays were installed in a contiguous area on a section of the greenhouse roof, decreasing the transmittance of all solar radiation wavelengths and photosynthetically active radiation (PAR) wavelengths (400–700 nm) to the OPV-shaded area by approximately 40% and 37%, respectively. Microclimate conditions and tomato crop growth and yield parameters were measured in both the OPV-shaded (‘OPV’) and non-OPV-shaded (‘Control’) sections of the greenhouse. The OPV shade stabilized the canopy temperature during midday periods with the highest solar radiation intensities, performing the function of a conventional shading method. Although delayed fruit development and ripening in the OPV section resulted in lower total yields compared to the Control section (24.6 kg m−2 and 27.7 kg m−2, respectively), after the fourth (of 10 total) harvests, the average weekly yield, fruit number, and fruit mass were not significantly different between the treatment (OPV-shaded) and control group. Light use efficiency (LUE), defined as the ratio of total fruit yield to accumulated PAR received by the plant canopy, was nearly twice as high as the Control section, with 21.4 g of fruit per mole of PAR for plants in the OPV-covered section compared to 10.1 g in the Control section. Overall, this study demonstrated that the use of semi-transparent OPVs as a seasonal shade element for greenhouse production in a high-light region is feasible. However, a higher transmission of PAR and greater OPV device efficiency and durability could make OPV shades more economically viable, providing a desirable solution for co-located greenhouse crop production and renewable energy generation in hot and high-light intensity regions.


2021 ◽  
Vol 7 (3) ◽  
pp. 194
Author(s):  
Carmen Gómez-Lama Cabanás ◽  
Antonio J. Fernández-González ◽  
Martina Cardoni ◽  
Antonio Valverde-Corredor ◽  
Javier López-Cepero ◽  
...  

This study aimed to disentangle the structure, composition, and co-occurrence relationships of the banana (cv. Dwarf Cavendish) root endophytome comparing two phenological plant stages: mother plants and suckers. Moreover, a collection of culturable root endophytes (>1000) was also generated from Canary Islands. In vitro antagonism assays against Fusarium oxysporum f.sp. cubense (Foc) races STR4 and TR4 enabled the identification and characterization of potential biocontrol agents (BCA). Eventually, three of them were selected and evaluated against Fusarium wilt of banana (FWB) together with the well-known BCA Pseudomonas simiae PICF7 under controlled conditions. Culturable and non-culturable (high-throughput sequencing) approaches provided concordant information and showed low microbial diversity within the banana root endosphere. Pseudomonas appeared as the dominant genus and seemed to play an important role in the banana root endophytic microbiome according to co-occurrence networks. Fungal communities were dominated by the genera Ophioceras, Cyphellophora, Plecosphaerella, and Fusarium. Overall, significant differences were found between mother plants and suckers, suggesting that the phenological stage determines the recruitment and organization of the endophytic microbiome. While selected native banana endophytes showed clear antagonism against Foc strains, their biocontrol performance against FWB did not improve the outcome observed for a non-indigenous reference BCA (strain PICF7).


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