scholarly journals Synergistic Regulation of Nitrogen and Sulfur on Redox Balance of Maize Leaves and Amino Acids Balance of Grains

2020 ◽  
Vol 11 ◽  
Author(s):  
Shuoran Liu ◽  
Shuai Cui ◽  
Xue Zhang ◽  
Yin Wang ◽  
Guohua Mi ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Nutritional Quality ◽  
Protein Concentration ◽  
Maize Yield ◽  
Redox Balance ◽  
Essential Amino Acids ◽  
Grain Protein ◽  
Grain Protein Concentration ◽  
Maize Leaves

As a primary food crop, maize is widely grown around the world. However, the deficiency of essential amino acids, such as lysine, tryptophan, and methionine, results in poor nutritional quality of maize. In addition, the protein concentration of maize declines with the increase in yield, which further reduces the nutritional quality. Here, the photosynthesis of leaves, grain amino acid composition, and stoichiometry of N and S are explored. The results show that N and S maintained the redox balance by increasing the content of glutathione in maize leaves, thereby enhancing the photosynthetic rate and maize yield. Simultaneously, the synergy of N and S increased the grain protein concentration and promoted amino acid balance by increasing the cysteine concentration in maize grains. The maize yield, grain protein concentration, and concentration of essential amino acids, such as lysine, tryptophan, and methionine, could be simultaneously increased in the N:S ratio range of 11.0 to 12.0. Overall, the synergy of N and S simultaneously improved the maize yield and nutritional quality by regulating the redox balance of maize leaves and the amino acids balance of grains, which provides a new theoretical basis and practical method for sustainable production of maize.

scholarly journals Nutritional quality evaluation of Whitemouth croaker (Micropogonias furnieri) protein isolate

2014 ◽  
Vol 44 (2) ◽  
pp. 134-143
Author(s):  
William Renzo Cortez-Vega ◽  
Irene Rodrigues Freitas ◽  
Sandriane Pizato ◽  
Carlos Prentice
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Nutritional Quality ◽  
Troponin I ◽  
Essential Amino Acids ◽  
Protein Isolate ◽  
Content Type ◽  
Sds Page ◽  
Apparent Bioavailability

Purpose – The purpose of this study was to isolate Whitemouth croaker protein by alkaline solubilization process and evaluate their nutritional quality to evaluate the bioavailability of essential amino acids. Design/methodology/approach – The proximate composition, essential amino acid composition, in vitro digestibility, apparent bioavailability, chemical score of amino acids and SDS-PAGE were determined for the isolated croaker proteins. Findings – The isolated protein showed a high level of protein 92.21 percent and low amount of lipids 0.57 percent. The protein is rich in lysine and leucine, 108.73 and 96.75 mg/g protein, respectively. The protein isolate had high digestibility, 94.32 percent, which indicates proper utilization of this protein source, while the tryptophan had lower bioavailability (12.58 mg amino acid/mg protein). The high chemical scores were found for the amino acids lysine, methionine+cysteine (6.79 and 5.14). SDS-PAGE of proteins extracted showed appearance of the heavy chain of myosin (220 kDa), actin (50 kDa) and other fractions, with molecular weight between 20 and 50 kDa, such as troponin I, C and T. Originality/value – The products obtained from croaker muscle can be incorporated as a high value supplements in human diets. The isolated protein exhibited a high content of essential amino acids and digestibility, indicating that the protein has a high nutritional quality.

Nutritional quality evaluation of surimi and kamaboko obtained from mechanically separated chicken meat

2014 ◽  
Vol 44 (6) ◽  
pp. 483-491 ◽  
Author(s):  
William Renzo Cortez-Vega ◽  
Sandriane Pizato ◽  
Carlos Prentice
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Nutritional Quality ◽  
Essential Amino Acids ◽  
Chicken Meat ◽  
Content Type ◽  
Minimum Requirement ◽  
Limiting Amino Acid ◽  
Apparent Bioavailability

Purpose – The purpose of this paper was to determine the nutritional quality of the surimi and kamaboko obtained from mechanically separated chicken meat and evaluate the bioavailability of essential amino acids found in these products. Design/methodology/approach – The mechanically separated chicken meat (MSCM) was characterized by the proximate composition, and the surimi and kamaboko were characterized by in vitro digestibility, determination of chemical score of amino acids and apparent bioavailability. Findings – The MSCM contains 68.1 ± 0.5, 12.9 ± 0.24, 18.5 ± 0.28 and 0.6 ± 0.06 per cent moisture, protein, lipids and ash, respectively. The moisture of the MSCM (surimi) was 80.45 ± 0.15 per cent, and the protein was 10.04 ± 0.21 per cent. The highest digestibility was found for the kamaboko (92.27 per cent) which was heat-treated and the lowest was for surimi (90.82 per cent). Histidine is a limiting amino acid. In this study, the surimi showed 84.69 per cent and the kamaboko presented 81.31 per cent of the minimum requirement for adults. In relation to the apparent bioavailability, there was a decrease of surimi to kamaboko of 2.52 per cent of the limiting amino acid histidine. The surimi and the kamaboko presented 76.94 and 75 per cent of the minimum requirement for adults, respectively. Originality/value – The application of the surimi technology in the production of a surimi-like material from mechanically deboned chicken meat provides a new approach toward increasing its value and utilization, e.g. for the development of meat-based products and analogs, as alternative protein sources, the surimi and the kamaboko exhibited a high content of essential amino acids, indicating that the protein has a relatively high nutritional quality.

Screening of amino acid constituents from date palm fruits

2016 ◽  
Vol 5 (10) ◽  
pp. 4972
Author(s):  
Lata Birlangi
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
United Arab Emirates ◽  
Nutritional Value ◽  
Date Palm ◽  
Amino Acid Content ◽  
Essential Amino Acids ◽  
Cultivated Plants ◽  
Palm Fruits ◽  
Social Part

The date palm (Phoenix dactylifera L.) is one of mankind’s oldest cultivated plants. The fruit of the date palm is an important crop of the hot arid and semi-arid regions of the world. It has always played a genuine economic and social part in the lives of the people of these areas. The present objective in examining the amino acid content of different varieties of date palm fruits from Middle-East region; is to determine whether its protein could effectively supplement the nutritional value and it is also aimed in finding which variety is rich in number of amino acids. The phytochemical screening revealed the presence of eight essential amino acids and five non-essential amino acids in the date fruits. Among all the date fruit varieties taken as samples for the study, Dabbas cultivar of United Arab Emirates found to exhibit eight types of amino acids which includes five as non-essential ones. Total of thirteen amino acids were detected in the seven date cultivars. Determination of amino acid can serve as a guide to the possible nutritional value.

Genetic Relationship between Kernel Discoloration and Grain Protein Concentration in Barley

Crop Science ◽  
2003 ◽  
Vol 43 (5) ◽  
pp. 1671-1679 ◽  
Author(s):  
Paulo C. Canci ◽  
Lexingtons M. Nduulu ◽  
Ruth Dill‐Macky ◽  
Gary J. Muehlbauer ◽  
Donald C. Rasmusson ◽  
...  
Keyword(s):  
Genetic Relationship ◽  
Protein Concentration ◽  
Grain Protein ◽  
Grain Protein Concentration ◽  
Kernel Discoloration

scholarly journals Progress towards reduced-crude protein diets for broiler chickens and sustainable chicken-meat production

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Sonia Yun Liu ◽  
Shemil P. Macelline ◽  
Peter V. Chrystal ◽  
Peter H. Selle
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Broiler Chickens ◽  
Crude Protein ◽  
Plasma Concentrations ◽  
Essential Amino Acids ◽  
Chicken Meat ◽  
Meat Production ◽  
Accurate Identification ◽  
Reduced Crude Protein

AbstractThe prime purpose of this review is to explore the pathways whereby progress towards reduced-crude protein (CP) diets and sustainable chicken-meat production may be best achieved. Reduced-CP broiler diets have the potential to attenuate environmental pollution from nitrogen and ammonia emissions; moreover, they have the capacity to diminish the global chicken-meat industry’s dependence on soybean meal to tangible extents. The variable impacts of reduced-CP broiler diets on apparent amino acid digestibility coefficients are addressed. The more accurate identification of amino acid requirements for broiler chickens offered reduced-CP diets is essential as this would diminish amino acid imbalances and the deamination of surplus amino acids. Deamination of amino acids increases the synthesis and excretion of uric acid for which there is a requirement for glycine, this emphasises the value of so-called “non-essential” amino acids. Starch digestive dynamics and their possible impact of glucose on pancreatic secretions of insulin are discussed, although the functions of insulin in avian species require clarification. Maize is probably a superior feed grain to wheat as the basis of reduced-CP diets; if so, the identification of the underlying reasons for this difference should be instructive. Moderating increases in starch concentrations and condensing dietary starch:protein ratios in reduced-CP diets may prove to be advantageous as expanding ratios appear to be aligned to inferior broiler performance. Threonine is specifically examined because elevated free threonine plasma concentrations in birds offered reduced-CP diets may be indicative of compromised performance. If progress in these directions can be realised, then the prospects of reduced-CP diets contributing to sustainable chicken-meat production are promising.

scholarly journals Further evidence that mechanisms of host/symbiont integration are dissimilar in the maternal versus embryonic Acyrthosiphon pisum bacteriome

EvoDevo ◽  
2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Celeste R. Banfill ◽  
Alex C. C. Wilson ◽  
Hsiao-ling Lu
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Acyrthosiphon Pisum ◽  
Essential Amino Acids ◽  
Developmental Time ◽  
Follicular Epithelium ◽  
Future Research ◽  
Buchnera Aphidicola ◽  
Bacterial Endosymbionts ◽  
Asexual Embryogenesis

Abstract Background Host/symbiont integration is a signature of evolutionarily ancient, obligate endosymbioses. However, little is known about the cellular and developmental mechanisms of host/symbiont integration at the molecular level. Many insects possess obligate bacterial endosymbionts that provide essential nutrients. To advance understanding of the developmental and metabolic integration of hosts and endosymbionts, we track the localization of a non-essential amino acid transporter, ApNEAAT1, across asexual embryogenesis in the aphid, Acyrthosiphon pisum. Previous work in adult bacteriomes revealed that ApNEAAT1 functions to exchange non-essential amino acids at the A. pisum/Buchnera aphidicola symbiotic interface. Driven by amino acid concentration gradients, ApNEAAT1 moves proline, serine, and alanine from A. pisum to Buchnera and cysteine from Buchnera to A. pisum. Here, we test the hypothesis that ApNEAAT1 is localized to the symbiotic interface during asexual embryogenesis. Results During A. pisum asexual embryogenesis, ApNEAAT1 does not localize to the symbiotic interface. We observed ApNEAAT1 localization to the maternal follicular epithelium, the germline, and, in late-stage embryos, to anterior neural structures and insect immune cells (hemocytes). We predict that ApNEAAT1 provisions non-essential amino acids to developing oocytes and embryos, as well as to the brain and related neural structures. Additionally, ApNEAAT1 may perform roles related to host immunity. Conclusions Our work provides further evidence that the embryonic and adult bacteriomes of asexual A. pisum are not equivalent. Future research is needed to elucidate the developmental time point at which the bacteriome reaches maturity.

The screening and preliminary construction of quality mutant population for cultivar “Nipponbare” in japonica rice (Oryza sativa)

Biologia ◽  
2012 ◽  
Vol 67 (6) ◽  
Author(s):  
Da Zhang ◽  
Jian Wu ◽  
Guan Li ◽  
Chun Shi
Keyword(s):  
Amino Acids ◽  
Oryza Sativa ◽  
Amino Acid ◽  
Mutation Frequency ◽  
Near Infrared ◽  
Essential Amino Acids ◽  
Japonica Rice ◽  
Near Infrared Reflectance ◽  
Mutant Population ◽  
Nonessential Amino Acids

AbstractProgenies of Oryza sativa cv. Nipponbare induced with 0.4% ethyl methane sulphonate (EMS) were screened for quality mutants and the preliminary quality mutant population was constructed in present experiment. A total of 2210 materials were first screened using near infrared reflectance spectroscopy (NIRS) from which 208 quality mutants were obtained for a second screening and then yielded 73 quality mutants including amylase content (AC), gel consistency (GC), gelatinization temperature (GT), protein content (PC), rapid viscosity analysis (RVA) parameters and amino acid contents. The screening yielded 11 PC mutants with a mutation frequency of 4.98‰, followed by 7 rice floury viscosity mutants (3.17‰), 5 AC mutants (2.26‰), 4 chalky mutants, GT and GC mutants (1.81‰), and 2 ASV mutants (0.9‰). The relative contents of 17 kinds of amino acid mutations, including 7 kinds for essential amino acids and 10 kinds for nonessential amino acids were identified. With the variation of 10% as the screening standard, mutants were obtained for lysine and leucine at 0.45‰ and for valine at 4.98‰, but no mutants were found for isoleucine, phenylalanine, threonine. For nonessential amino acids, mutants of glutamic (0.45‰), arginine (3.62‰), alanine (3.17‰), serine (0.45‰), glycine (0.45‰), tyrosine (1.81‰), proline (2.71‰), and histidine (0.45‰) were obtained, but none was found for aspartic, phenylalanine nor threonine. At 100% as the screening standard for methionine and cysteines, the mutation frequency of these two amino acid mutants were 0.9‰ and 4.98‰ respectively. Quality mutants in this preliminary library of rice could play important role in gene function and breeding of rice quality.

Crop and soil nitrogen status of tilled and no-tillage systems in semiarid regions of Saskatchewan

2002 ◽  
Vol 82 (4) ◽  
pp. 489-498 ◽  
Author(s):  
B G McConkey ◽  
D. Curtin ◽  
C A Campbell ◽  
S A Brandt ◽  
F. Selles
Keyword(s):  
Grain Yield ◽  
Protein Concentration ◽  
Grain Protein ◽  
Soil N ◽  
Grain Protein Concentration ◽  
Brown Soil ◽  
Semiarid Regions ◽  
Soil Zone ◽  
Tillage System ◽  
Loam Soil

We examined 1990-1996 crop and soil N data for no-tillage (NT), minimum tillage (MT) and conventional tillage (CT) systems from four long-term tillage studies in semiarid regions of Saskatchewan for evidence that the N status was affected by tillage system. On a silt loam and clay soil in the Brown soil zone, spring what (Triticum aestivum L.) grain yield and protein concentration were lower for NT compared with tilled (CT or MT) systems for a fallow-wheat (F-WM) rotation. Grain protein concentration for continuous wheat (Cont W) was also lower for NT than for MT. For a sandy loam soil in the Brown soil zone, durum (Triticum durum L.) grain protein concentration was similar for MT and NT for both Cont W and F-W, but NT had higher grain yield than MT (P < 0.05 for F-W only). For a loam soil in the Dark Brown soil zone, wheat grain yield for NT was increased by about 7% for fallow-oilseed-wheat (F-O-W) and wheat-oilseed-wheat (W-O-W) rotations. The higher grain yields for NT reduced grain protein concentration by dilution effect as indicated by similar grain N yield. However, at this site, about 23 kg ha-1 more fertilizer N was required for NT than for CT. Elimination of tillage increased total organic N in the upper 7.5 cm of soil and N in surface residues. Our results suggest that a contributing factor to decreased availability of soil N in medium- and fine-textured soils under NT was a slower rate of net N mineralization from organic matter. Soil nitrates to 2.4 m depth did not indicate that nitrate leaching was affected by tillage system. Current fertilizer N recommendations developed for tilled systems may be inadequate for optimum production of wheat with acceptable grain protein under NT is semiarid regions of Saskatchewan. Key words: Tillage intensity, N availability, soil N fractions, N mineralization, crop residue decomposition, grain protein

Effect of leaf spotting diseases on grain yield and seed traits of wheat in southern Saskatchewan

2002 ◽  
Vol 82 (3) ◽  
pp. 507-512 ◽  
Author(s):  
H. Wang ◽  
M. R. Fernandez ◽  
F. R. Clarke ◽  
R. M. DePauw ◽  
J. M. Clarke
Keyword(s):  
Grain Yield ◽  
Protein Concentration ◽  
Flag Leaf ◽  
Kernel Weight ◽  
Test Weight ◽  
Grain Protein ◽  
Seed Traits ◽  
Grain Protein Concentration ◽  
Leaf Emergence ◽  
Weight Test

Although leaf spotting diseases have been reported to have a negative effect on grain yield and seed characteristics of wheat (Triticum spp.), the magnitude of such effects on wheat grown on dryland in southern Saskatchewan is not known. A fungicide experiment was conducted at Swift Current (Brown soil) and Indian Head (Black soil) from 1997 to 1999 to determine the effect of leaf spotting diseases on yield and seed traits of wheat. Two fungicides, Folicur 3.6F and Bravo 500, were applied at different growth stages on three common wheat (Triticum aestivum L.) and three durum wheat (T. turgidum L. var durum) genotypes. Fungicide treatments generally did not affect yield, kernel weight, test weight or grain protein concentration, and these effects were relatively consistent among genotypes. Folicur applied at head emergence in 1997 and at flag leaf emergence and/or head emergence in 1998 increased yield at Indian Head (P < 0.05). Fungicides applied at and before flag leaf emergence tended to increase kernel weight. Grain protein concentration increased only in treatments of Bravo applications at Indian Head in 1998. These results suggested that under the dryland environment and management in southern Saskatchewan leaf spotting diseases generally have a small effect on yield, kernel weight, test weight and protein concentration. Key words: Wheat, leaf spotting diseases, fungicide, yield

Effect of weeds and nitrogen fertiliser on yield and grain protein concentration of wheat

1996 ◽  
Vol 36 (4) ◽  
pp. 443 ◽  
Author(s):  
MG Mason ◽  
RW Madin
Keyword(s):  
Grain Yield ◽  
Weed Control ◽  
Dry Matter ◽  
Protein Concentration ◽  
The Other ◽  
Grain Protein ◽  
Dry Matter Yield ◽  
Wheat Grain ◽  
Grain Protein Concentration ◽  
Nitrogen Fertiliser

Field trials at Beverley (19911, Salmon Gums (1991; 2 sites) and Merredin (1992; 2 sites), each with 5 rates of nitrogen (N) and 3 levels of weed control, were used to investigate the effect of weeds and N on wheat grain yield and protein concentration during 1991 and 1992. Weeds in the study were grasses (G) and broadleaf (BL). Weeds reduced both vegetative dry matter yield and grain yield of wheat at all sites except for dry matter at Merredin (BL). Nitrogen fertiliser increased wheat dry matter yield at all sites. Nitrogen increased wheat grain yield at Beverley and Merredin (BL), but decreased yield at both Salmon Gums sites in 1991. Nitrogen fertiliser increased grain protein concentration at all 5 sites-at all rates for 3 sites [Salmon Gums (G) and (BL) and Merredin (G)] and at rates of 69 kg N/ha or more at the other 2 sites [Beverley and Merredin (BL)]. However, the effect of weeds on grain protein varied across sites. At Merredin (G) protein concentration was higher where there was no weed control, possibly due to competition for soil moisture by the greater weed burden. At Salmon Gums (G), grain protein concentration was greater when weeds were controlled than in the presence of weeds, probably due to competition for N between crop and weeds. In the other 3 trials, there was no effect of weeds on grain protein. The effect of weeds on grain protein appears complex and depends on competition between crop and weeds for N and for water at the end of the season, and the interaction between the two.

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