scholarly journals The Resistance of Oilseed Rape Microspore-Derived Embryos to Osmotic Stress Is Associated With the Accumulation of Energy Metabolism Proteins, Redox Homeostasis, Higher Abscisic Acid, and Cytokinin Contents

2021 ◽  
Vol 12 ◽  
Author(s):  
Milan O. Urban ◽  
Sébastien Planchon ◽  
Irena Hoštičková ◽  
Radomira Vanková ◽  
Peter Dobrev ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Osmotic Stress ◽  
Functional Groups ◽  
Cell Structure ◽  
Protein Profile ◽  
Redox Homeostasis ◽  
Biomass Accumulation ◽  
Protein Accumulation ◽  
Suitable Model ◽  
Leaf Proteome

The present study aims to investigate the response of rapeseed microspore-derived embryos (MDE) to osmotic stress at the proteome level. The PEG-induced osmotic stress was studied in the cotyledonary stage of MDE of two genotypes: Cadeli (D) and Viking (V), previously reported to exhibit contrasting leaf proteome responses under drought. Two-dimensional difference gel electrophoresis (2D-DIGE) revealed 156 representative protein spots that have been selected for MALDI-TOF/TOF analysis. Sixty-three proteins have been successfully identified and divided into eight functional groups. Data are available via ProteomeXchange with identifier PXD024552. Eight selected protein accumulation trends were compared with real-time quantitative PCR (RT-qPCR). Biomass accumulation in treated D was significantly higher (3-fold) than in V, which indicates D is resistant to osmotic stress. Cultivar D displayed resistance strategy by the accumulation of proteins in energy metabolism, redox homeostasis, protein destination, and signaling functional groups, high ABA, and active cytokinins (CKs) contents. In contrast, the V protein profile displayed high requirements of energy and nutrients with a significant number of stress-related proteins and cell structure changes accompanied by quick downregulation of active CKs, as well as salicylic and jasmonic acids. Genes that were suitable for gene-targeting showed significantly higher expression in treated samples and were identified as phospholipase D alpha, peroxiredoxin antioxidant, and lactoylglutathione lyase. The MDE proteome profile has been compared with the leaf proteome evaluated in our previous study. Different mechanisms to cope with osmotic stress were revealed between the genotypes studied. This proteomic study is the first step to validate MDE as a suitable model for follow-up research on the characterization of new crossings and can be used for preselection of resistant genotypes.

scholarly journals Temporal regulation of proteome profile in the fruit fly, Drosophila melanogaster

2016 ◽  
Author(s):  
Perumal Subramanian ◽  
Jaime J Jayapalan ◽  
Puteri Abdul-Rahman ◽  
Manjula Arumugam ◽  
Onn Hashim
Keyword(s):  
Protein Synthesis ◽  
Drosophila Melanogaster ◽  
Protein Profile ◽  
Redox Homeostasis ◽  
Biological Clock ◽  
Fruit Fly ◽  
Trypsin Digestion ◽  
Diurnal Rhythms ◽  
Protein Accumulation ◽  
Ms Analysis

Background. Diurnal rhythms of protein synthesis controlled by the biological clock underlie rhythmic physiology in the fruit fly, Drosophila melanogaster. Self-sustained autonomous circadian oscillations were documented all over the organs of the fly. In this study, we conducted a proteome-wide investigation of rhythmic protein accumulation in D. melanogaster. Materials and Methods. We have used the whole fly for the proteomic study as performed in typical proteotypic peptide (PTP) studies and followed the same protocol with trypsin digestion. Total protein collected from fly samples harvested at 4h intervals over the 24-h period were subjected to two dimensional (2-D) gel electrophoresis, trypsin digestion and MS/MS analysis. Protein spots/clusters were identified with MASCOT search engine and Swiss-Prot database. Expression of proteins was documented as percentage of volume contribution using the Image Master 2D Platinum software. Results. A total of 124 protein spots/clusters were identified using MS/MS analysis. A significant variation in the expression of 88 proteins over the 24-h period was observed. Our present results suggested that the synthesis/regulation of numerous proteins is regulated by the biological clock in D. melanogaster. Relatively higher number of proteins was upregulated during nighttime as compared to daytime. Conclusion. As these rhythmically varying proteins/enzymes involve in metabolism, muscle activities, ion channels, protein synthesis, redox homeostasis and apoptosis our results indicate that these cellular processes could be regulated at the level of temporal expression of protein profile.

scholarly journals Temporal regulation of proteome profile in the fruit fly, Drosophila melanogaster

2016 ◽  
Author(s):  
Perumal Subramanian ◽  
Jaime J Jayapalan ◽  
Puteri Abdul-Rahman ◽  
Manjula Arumugam ◽  
Onn Hashim
Keyword(s):  
Protein Synthesis ◽  
Drosophila Melanogaster ◽  
Protein Profile ◽  
Redox Homeostasis ◽  
Biological Clock ◽  
Fruit Fly ◽  
Trypsin Digestion ◽  
Diurnal Rhythms ◽  
Protein Accumulation ◽  
Ms Analysis

Background. Diurnal rhythms of protein synthesis controlled by the biological clock underlie rhythmic physiology in the fruit fly, Drosophila melanogaster. Self-sustained autonomous circadian oscillations were documented all over the organs of the fly. In this study, we conducted a proteome-wide investigation of rhythmic protein accumulation in D. melanogaster. Materials and Methods. We have used the whole fly for the proteomic study as performed in typical proteotypic peptide (PTP) studies and followed the same protocol with trypsin digestion. Total protein collected from fly samples harvested at 4h intervals over the 24-h period were subjected to two dimensional (2-D) gel electrophoresis, trypsin digestion and MS/MS analysis. Protein spots/clusters were identified with MASCOT search engine and Swiss-Prot database. Expression of proteins was documented as percentage of volume contribution using the Image Master 2D Platinum software. Results. A total of 124 protein spots/clusters were identified using MS/MS analysis. A significant variation in the expression of 88 proteins over the 24-h period was observed. Our present results suggested that the synthesis/regulation of numerous proteins is regulated by the biological clock in D. melanogaster. Relatively higher number of proteins was upregulated during nighttime as compared to daytime. Conclusion. As these rhythmically varying proteins/enzymes involve in metabolism, muscle activities, ion channels, protein synthesis, redox homeostasis and apoptosis our results indicate that these cellular processes could be regulated at the level of temporal expression of protein profile.

scholarly journals Effects of ibogaine treatment on redox homeostasis and energy metabolism in rat

IBRO Reports ◽  
2019 ◽  
Vol 7 ◽  
pp. 43
Author(s):  
N. Tatalović ◽  
T. Vidonja Uzelac ◽  
Z. Oreščanin Dušić ◽  
A. Nikolić Kokić ◽  
M. Spasić ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Redox Homeostasis

scholarly journals   Assimilatory function and biochemical changes in Stylosanthes hamata grown under elevated CO2

2012 ◽  
Vol 58 (No. 5) ◽  
pp. 224-229 ◽  
Author(s):  
M.J. Baig ◽  
R.K. Bhatt ◽  
H.S. Tiwari ◽  
P. Swami
Keyword(s):  
Biomass Production ◽  
Protein Profile ◽  
Photosynthetic Pigment ◽  
Protein Accumulation ◽  
Maximum Increase ◽  
Plant Parts ◽  
Stylosanthes Hamata ◽  
Use Efficiency ◽  
The Impact

We studied the impact of 360 &plusmn; 50 &micro;L/l (ambient) and 600 &plusmn; 50 &micro;L/L (elevated) CO<sub>2</sub> on growth performance, biomass production, photosynthetic efficiency, carbon isotope discrimination, protein profile and some antioxidant enzymes on Stylosanthes hamata. This crop responded significantly to photosynthetic rate, stomatal conductance and transpiration rate under elevated CO<sub>2</sub>. The biomass production in terms of fresh and dry was increased in elevated CO<sub>2</sub> by 126.81% (fresh) and 114.55% (dry) over ambient CO<sub>2</sub>. Long term exposure to elevated CO<sub>2</sub> enhanced photosynthetic water use efficiency by 127.77%. The photosynthetic pigment, total chlorophyll and chlorophyll a/b ratio also increased by 220.56 and 132.86%, respectively in elevated over ambient CO<sub>2</sub>. Around 149% increase in the soluble protein accumulation (mg/g FW) was recorded under elevated over ambient CO<sub>2</sub>, which was also reflected in the polyacrylamide gel profile. The isoforms of superoxide dismutase and esterase isozymes showed remarkable difference under elevated as compared to ambient. Measurement of <sup>13</sup>&delta; in different plant parts indicated a significant increase in discrimination against <sup>13</sup>C when plants were grown at elevated relative to ambient CO<sub>2</sub>. Maximum increase was recorded in roots (439.72%) followed by leaf and the stem recorded least increase in <sup>13</sup>&delta; (119.94%) in elevated over ambient CO<sub>2</sub>. &nbsp; &nbsp;

Growth and 2-methylisoborneol production by the cyanobacterium Phormidium LM689

1995 ◽  
Vol 31 (11) ◽  
pp. 181-186 ◽  
Author(s):  
W. J. Zimmerman ◽  
C. M. Soliman ◽  
B. H. Rosen
Keyword(s):  
Copper Sulfate ◽  
Soluble Protein ◽  
Organic Amendments ◽  
Biomass Accumulation ◽  
Protein Accumulation ◽  
Total Chlorophyll ◽  
Free Medium ◽  
Chlorophyll Accumulation ◽  
Antibiotic Effect ◽  
Pigment Biosynthesis

Growth and production of 2-methylisoborneol (MIB) was characterized in the cyanobacterium Phormidium sp. LM689 isolated from Lake Mathews, California, USA. The effects of: 1) uptake of several organic amendments related to pigment biosynthesis, 2) Fe availability, and 3) copper algicides were examined. Growth was estimated by total chlorophyll and soluble protein accumulation. Pyruvate additions up to 1250 mM did not effectively alter biomass accumulation. Mevalonic lactone increasingly stimulated (protein) growth but not MIB output at the same range of concentrations. Geraniol did not inhibit overall cyanobacterial growth when added to 300 μM, but did slightly decrease chlorophyll accumulation. Farnesol exhibited an antibiotic effect at all concentrations from 10 to 100 μM. Phormidium was tolerant to 1 ppm Cu+2 added as copper sulfate or as chelated copper. Siderophore production and growth in Fe and chelator-free medium was also demonstrated.

scholarly journals Chloroplast Electron Chain, ROS Production, and Redox Homeostasis Are Modulated by COS-OGA Elicitation in Tomato (Solanum lycopersicum) Leaves

2020 ◽  
Vol 11 ◽  
Author(s):  
Sophie Moreau ◽  
Géraldine van Aubel ◽  
Rekin’s Janky ◽  
Pierre Van Cutsem
Keyword(s):  
Electron Transport ◽  
Electron Transport Chain ◽  
Redox Homeostasis ◽  
Crop Protection ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Biomass Accumulation ◽  
Photochemical Quenching ◽  
Fluorescence Measurements ◽  
Transport Chain ◽  
Reduced Nicotinamide Adenine Dinucleotide

The stimulation of plant innate immunity by elicitors is an emerging technique in agriculture that contributes more and more to residue-free crop protection. Here, we used RNA-sequencing to study gene transcription in tomato leaves treated three times with the chitooligosaccharides–oligogalacturonides (COS-OGA) elicitor FytoSave® that induces plants to fend off against biotrophic pathogens. Results showed a clear upregulation of sequences that code for chloroplast proteins of the electron transport chain, especially Photosystem I (PSI) and ferredoxin. Concomitantly, stomatal conductance decreased by half, reduced nicotinamide adenine dinucleotide phosphate [NAD(P)H] content and reactive oxygen species production doubled, but fresh and dry weights were unaffected. Chlorophyll, β-carotene, violaxanthin, and neoxanthin contents decreased consistently upon repeated elicitations. Fluorescence measurements indicated a transient decrease of the effective PSII quantum yield and a non-photochemical quenching increase but only after the first spraying. Taken together, this suggests that plant defense induction by COS-OGA induces a long-term acclimation mechanism and increases the role of the electron transport chain of the chloroplast to supply electrons needed to mount defenses targeted to the apoplast without compromising biomass accumulation.

scholarly journals Predicting plant biomass accumulation from image-derived parameters

2016 ◽  
Author(s):  
Dijun Chen ◽  
Rongli Shi ◽  
Jean-Michel Pape ◽  
Christian Klukas
Keyword(s):  
High Throughput ◽  
Plant Biomass ◽  
Quantitative Relationship ◽  
Biomass Accumulation ◽  
Plant Phenotyping ◽  
Suitable Model ◽  
The European Union ◽  
Relative Contribution ◽  
Crop Plant Research ◽  
High Throughput Phenotyping

AbstractImage-based high-throughput phenotyping technologies have been rapidly developed in plant science recently and they provide a great potential to gain more valuable information than traditionally destructive methods. Predicting plant biomass is regarded as a key purpose for plant breeders and ecologist. However, it is a great challenge to find a suitable model to predict plant biomass in the context of high-throughput phenotyping. In the present study, we constructed several models to examine the quantitative relationship between image-based features and plant biomass accumulation. Our methodology has been applied to three consecutive barley experiments with control and stress treatments. The results proved that plant biomass can be accurately predicted from image-based parameters using a random forest model. The high prediction accuracy based on this model, in particular the cross-experiment performance, is promising to relieve the phenotyping bottleneck in biomass measurement in breeding applications. The relative contribution of individual features for predicting biomass was further quantified, revealing new insights into the phenotypic determinants of plant biomass outcome. What’s more, the methods could also be used to determine the most important image-based features related to plant biomass accumulation, which would be promising for subsequent genetic mapping to uncover the genetic basis of biomass.One-sentence SummaryWe demonstrated that plant biomass can be accurately predicted from image-based parameters in the context of high-throughput phenotyping.FootnotesThis work was supported by the Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), the Robert Bosch Stiftung (32.5.8003.0116.0) and the Federal Agency for Agriculture and Food (BEL, 15/12-13, 530-06.01-BiKo CHN) and the Federal Ministry of Education and Research (BMBF, 0315958A and 031A053B). This research was furthermore enabled with support of the European Plant Phenotyping Network (EPPN, grant agreement no. 284443) funded by the FP7 Research Infrastructures Programme of the European Union.

scholarly journals Alterations in Energy Metabolism, Mitochondrial Function and Redox Homeostasis in GK Diabetic Rat Tissues Treated with Aspirin

Life ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 104
Author(s):  
Annie John ◽  
Layla Amiri ◽  
Jasmin Shafarin ◽  
Saeed Tariq ◽  
Ernest Adeghate ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Energy Metabolism ◽  
Mitochondrial Function ◽  
Redox Homeostasis ◽  
Diabetic Rats ◽  
Endocrine Function ◽  
Diabetic Rat ◽  
Rat Tissues ◽  
Gk Rats ◽  
And Oxidative Stress

Our recent studies have demonstrated that aspirin treatment prevents inflammatory and oxidative stress-induced alterations in mitochondrial function, improves glucose tolerance and pancreatic endocrine function and preserves tissue-specific glutathione (GSH)-dependent redox homeostasis in Goto-Kakizaki (GK) diabetic rats. In the current study, we have investigated the mechanism of action of aspirin in maintaining mitochondrial bioenergetics and redox metabolism in the liver and kidneys of GK rats. Aspirin reduced the production of reactive oxygen species (ROS) and oxidative stress-induced changes in GSH metabolism. Aspirin treatment also improved mitochondrial respiratory function and energy metabolism, in addition to regulating the expression of cell signaling proteins that were altered in diabetic animals. Ultrastructural electron microscopy studies revealed decreased accumulation of glycogen in the liver of aspirin-treated diabetic rats. Hypertrophic podocytes with irregular fusion of foot processes in the renal glomerulus and detached microvilli, condensed nuclei and degenerated mitochondria observed in the proximal convoluted tubules of GK rats were partially restored by aspirin. These results provide additional evidence to support our previous observation of moderation of diabetic complications by aspirin treatment in GK rats and may have implications for cautious use of aspirin in the therapeutic management of diabetes.

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