scholarly journals Membrane Lipids’ Metabolism and Transcriptional Regulation in Maize Roots Under Cold Stress

2021 ◽  
Vol 12 ◽  
Author(s):  
Xunchao Zhao ◽  
Yulei Wei ◽  
Jinjie Zhang ◽  
Li Yang ◽  
Xinyu Liu ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Low Temperature ◽  
Cold Stress ◽  
Temperature Stress ◽  
Membrane Lipids ◽  
Membrane Lipid ◽  
Metabolic Changes ◽  
Low Temperature Stress ◽  
Maize Seedlings ◽  
Maize Roots

Low temperature is one of the major abiotic stresses that restrict the growth and development of maize seedlings. Membrane lipid metabolism and remodeling are key strategies for plants to cope with temperature stresses. In this study, an integrated lipidomic and transcriptomic analysis was performed to explore the metabolic changes of membrane lipids in the roots of maize seedlings under cold stress (5°C). The results revealed that major extraplastidic phospholipids [phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA), and phosphatidylinositol (PI)] were dominant membrane lipids in maize root tissues, accounting for more than 70% of the total lipids. In the transcriptome data of maize roots under cold stress, a total of 189 lipid-related differentially expressed genes (DEGs) were annotated and classified into various lipid metabolism pathways, and most of the DEGs were enriched in the “Eukaryotic phospholipid synthesis” (12%), “Fatty acid elongation” (12%), and “Phospholipid signaling” (13%) pathways. Under low temperature stress, the molar percentage of the most abundant phospholipid PC decreased around 10%. The significantly up-regulated expression of genes encoding phospholipase [phospholipase D (PLD)] and phosphatase PAP/LPP genes implied that PC turnover was triggered by cold stress mainly via the PLD pathway. Consequently, as the central product of PC turnover, the level of PA increased drastically (63.2%) compared with the control. The gene-metabolite network and co-expression network were constructed with the prominent lipid-related DEGs to illustrate the modular regulation of metabolic changes of membrane lipids. This study will help to explicate membrane lipid remodeling and the molecular regulation mechanism in field crops encountering low temperature stress.

scholarly journals Identification of cold stress responsive microRNAs in cold tolerant and susceptible Hemerocallis fulva by high throughput sequencing

2020 ◽  
Author(s):  
Changbing Huang ◽  
Chun Jiang ◽  
limin Jin ◽  
Huanchao Zhang
Keyword(s):  
Low Temperature ◽  
Cold Stress ◽  
Cold Tolerance ◽  
Temperature Stress ◽  
Stress Responses ◽  
Target Genes ◽  
Low Temperature Stress ◽  
Differentially Expressed ◽  
Differentially Expressed Mirnas ◽  
Cold Tolerant

Abstract Background:Hemerocallis fulva is a perennial herb belonging to Hemerocallis of Hemerocallis. Because of the large and bright colors, it is often used as a garden ornamental plant. But most varieties of H. fulva on the market will wither in winter, which will affect their beauty. It is very important to study the effect of low temperature stress on the physiological indexes of H. fulva and understand the cold tolerance of different H. fulva. MiRNA is a kind of endogenous non coding small molecular RNA with length of 21-24nt. It mainly inhibits protein translation by cutting target genes, and plays an important role in the development of organisms, gene expression and biological stress. Low temperature is the main abiotic stress affecting the production of H. fulva in China, which hinders the growth and development of plants. A comprehensive understanding of the expression pattern of microRNA in H. fulva under low temperature stress can improve our understanding of microRNA mediated stress response. Although there are many studies on miRNAs of various plants under cold stress at home and abroad, there are few studies on miRNAs related to cold stress of H. fulva. It is of great significance to explore the cold stress resistant gene resources of H. fulva, especially the identification and functional research of miRNA closely related to cold stress, for the breeding of excellent H. fulva.Results A total of 5619 cold-responsive miRNAs, 315 putative novel and 5 304 conserved miRNAs, were identified from the leaves and roots of two different varieties ‘Jinyan’ (cold-tolerant) and ‘Lucretius ’ (cold-sensitive), which were stressed under -4 oC for 24 h. Twelve conserved and three novel miRNAs (novel-miR10, novel-miR19 and novel-miR48) were differentially expressed in leaves of ‘Jinyan’ under cold stress. Novel-miR19, novel-miR29 and novel-miR30 were up-regulated in roots of ‘Jinyan’ under cold stress. Thirteen and two conserved miRNAs were deferentially expressed in leaves and roots of ‘Lucretius’ after cold stress. The deferentially expressed miRNAs between two cultivars under cold stress include novel miRNAs and the members of the miR156, miR166 and miR319 families. A total of 6 598 target genes for 6 516 known miRNAs and 82 novel miRNAs were predicted by bioinformatic analysis, mainly involved in metabolic processes and stress responses. Ten differentially expressed miRNAs and predicted target genes were confirmed by quantitative reverse transcription PCR(q-PCR), and the expressional changes of target genes were negatively correlated to differentially expressed miRNAs. Our data indicated that some candidate miRNAs (e.g., miR156a-3-p, miR319a, and novel-miR19) may play important roles in plant response to cold stress.Conclusions Our study indicates that some putative target genes and miRNA mediated metabolic processes and stress responses are significant to cold tolerance in H. fulva.

scholarly journals Exogenous diethyl aminoethyl hexanoate ameliorates low temperature stress by improving nitrogen metabolism in maize seedlings

PLoS ONE ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. e0232294
Author(s):  
Jianguo Zhang ◽  
Shujun Li ◽  
Quan Cai ◽  
Zhenhua Wang ◽  
Jingsheng Cao ◽  
...  
Keyword(s):  
Low Temperature ◽  
Nitrogen Metabolism ◽  
Temperature Stress ◽  
Low Temperature Stress ◽  
Maize Seedlings ◽  
Diethyl Aminoethyl Hexanoate

scholarly journals Gene expression and metabolic changes of Momordica charantia L. seedlings in response to low temperature stress

PLoS ONE ◽  
2020 ◽  
Vol 15 (5) ◽  
pp. e0233130
Author(s):  
Yu Niu ◽  
Ziji Liu ◽  
Huang He ◽  
Xu Han ◽  
Zhiqiang Qi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Low Temperature ◽  
Temperature Stress ◽  
Momordica Charantia ◽  
Metabolic Changes ◽  
Low Temperature Stress

scholarly journals Antioxidant Defense, Chlorophyll Fluorescence and VvCBF4-VvNAC1 Genes Expression in Grapevine Cultivars (Vitis Vinifera L.) in Response to Cold Stress

2021 ◽  
Author(s):  
M. A. Aazami ◽  
M. Asghari Aruq ◽  
M. B. Hassanpouraghdam
Keyword(s):  
Chlorophyll Fluorescence ◽  
Low Temperature ◽  
Cold Stress ◽  
Temperature Stress ◽  
Low Temperature Stress ◽  
Vitis Vinifera L ◽  
Agricultural Industry ◽  
Genes Expression ◽  
Significant Damage ◽  
Photochemical Quantum Yield

Abstract Background: Cold stress is one of the limitative factors of different species of crops on the planet, causing significant damage to the Iranian agricultural industry every year. Grapes are the product of temperate warm zones and sensitive to early autumn cold and spring cold. The current study the effects of cold stress (+1 °C for 4, 8, and 16 hours) on three grapevine cultivars (Ghiziluzum, Khalili, and Perllete) were investigated. Results: The results showed that cold stress caused significant changes in the antioxidant and biochemicals content in the studied cultivars. Furthermore, examining the chlorophyll fluorescence indices, cold stress caused a significant increase in minimal fluorescence (F0), a decrease in maximal fluorescence (Fm), and the maximum photochemical quantum yield of photosystem II (Fv/Fm) in all cultivars. According to the obtained results, among the three studied cultivars, ‘Perllete’ with the highest increase in proline content and the activity of antioxidant enzymes and also, having the lowest accumulation of malondialdehyde, hydrogen peroxide, electrolyte leakage, and F0 as well as less decrease in Fm and Fv/Fm had the higher tolerance to the cold stress than ‘Ghiziluzum’ and ‘Khalili’ cultivars. VvCBF4 and VvNAC1 genes expression was increased in all three cultivars at +1 °C at 8 hours and then decreased. The increase in VvCBF4 and VvNAC1 genes expression in ‘Perllete’ cultivar was higher than the other two cultivars. Conclusion: ‘Perllete’ and ‘Ghiziluzum’ showed the highest tolerance to low temperature stress, respectively. ‘Khalili’ was sensitive to low temperature stress.

scholarly journals The Methylation Patterns and Transcriptional Responses to Chilling Stress at the Seedling Stage in Rice

2019 ◽  
Vol 20 (20) ◽  
pp. 5089 ◽  
Author(s):  
Hui Guo ◽  
Tingkai Wu ◽  
Shuxing Li ◽  
Qiang He ◽  
Zhanlie Yang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Low Temperature ◽  
Cold Stress ◽  
Cold Tolerance ◽  
Temperature Stress ◽  
Chilling Stress ◽  
Low Temperature Stress ◽  
Expression Level ◽  
Altered Expression ◽  
Cold Tolerant

Chilling stress is considered the major abiotic stress affecting the growth, development, and yield of rice. To understand the transcriptomic responses and methylation regulation of rice in response to chilling stress, we analyzed a cold-tolerant variety of rice (Oryza sativa L. cv. P427). The physiological properties, transcriptome, and methylation of cold-tolerant P427 seedlings under low-temperature stress (2–3 °C) were investigated. We found that P427 exhibited enhanced tolerance to low temperature, likely via increasing antioxidant enzyme activity and promoting the accumulation of abscisic acid (ABA). The Methylated DNA Immunoprecipitation Sequencing (MeDIP-seq) data showed that the number of methylation-altered genes was highest in P427 (5496) and slightly lower in Nipponbare (Nip) and 9311 (4528 and 3341, respectively), and only 2.7% (292) of methylation genes were detected as common differentially methylated genes (DMGs) related to cold tolerance in the three varieties. Transcriptome analyses revealed that 1654 genes had specifically altered expression in P427 under cold stress. These genes mainly belonged to transcription factor families, such as Myeloblastosis (MYB), APETALA2/ethylene-responsive element binding proteins (AP2-EREBP), NAM-ATAF-CUC (NAC) and WRKY. Fifty-one genes showed simultaneous methylation and expression level changes. Quantitative RT-PCR (qRT-PCR) results showed that genes involved in the ICE (inducer of CBF expression)-CBF (C-repeat binding factor)—COR (cold-regulated) pathway were highly expressed under cold stress, including the WRKY genes. The homologous gene Os03g0610900 of the open stomatal 1 (OST1) in rice was obtained by evolutionary tree analysis. Methylation in Os03g0610900 gene promoter region decreased, and the expression level of Os03g0610900 increased, suggesting that cold stress may lead to demethylation and increased gene expression of Os03g0610900. The ICE-CBF-COR pathway plays a vital role in the cold tolerance of the rice cultivar P427. Overall, this study demonstrates the differences in methylation and gene expression levels of P427 in response to low-temperature stress, providing a foundation for further investigations of the relationship between environmental stress, DNA methylation, and gene expression in rice.

Gene-Coexpression Network Analysis Identifies Specific Modules and Hub Genes Related to Cold Stress in Rice

2021 ◽  
Author(s):  
Zhichi Zeng ◽  
Sichen Zhang ◽  
Wenyan Li ◽  
Baoshan Chen ◽  
Wenlan Li
Keyword(s):  
Network Analysis ◽  
Low Temperature ◽  
Cold Stress ◽  
Cold Tolerance ◽  
Temperature Stress ◽  
Low Temperature Stress ◽  
Rice Cultivars ◽  
Hub Genes ◽  
New Genes ◽  
Short Period

Abstract Background: When plants are subjected to cold stress, they undergo a series of molecular and physiological changes to protect themselves from injury. Indica cultivars can usually withstand only mild cold stress in a relatively short period. Hormone-mediated defence response plays an important role in cold stress. Weighted gene co-expression network analysis (WGCNA) is a very useful tool for studying the correlation between genes, identifying modules with high phenotype correlation, and identifying Hub genes in different modules. Many studies have elucidated the molecular mechanisms of cold tolerance in different plants, but little information about the recovery process after cold stress is available.Results: To understand the molecular mechanism of cold tolerance in rice, we performed comprehensive transcriptome analyses during cold treatment and recovery stage in two cultivars of near-isogenic lines (9311 and DC907). Twelve transcriptomes in two rice cultivars were determined. A total of 2509 new genes were predicted by fragment splicing and assembly, and 7506 differentially expressed genes were identified by pairwise comparison. A total of 26 modules were obtained by expression-network analysis, 12 of which were highly correlated with cold stress or recovery treatment. We further identified candidate Hub genes associated with specific modules and analysed their regulatory relationships based on coexpression data. Results showed that various plant-hormone regulatory genes acted together to protect plants from physiological damage under short-term low-temperature stress. We speculated that this may be common in rice. Under long-term cold stress, rice improved the tolerance to low-temperature stress by promoting autophagy, sugar synthesis, and metabolism.Conclusion: Through WGCNA analysis at the transcriptome level, we provided a potential regulatory mechanism for the cold stress and recovery of rice cultivars and identified candidate central genes. Our findings provided an important reference for the future cultivation of rice strains with good tolerance.

Exogenous application of 5-aminolevulinic acid improves low-temperature stress tolerance of maize seedlings

2018 ◽  
Vol 69 (6) ◽  
pp. 587 ◽  
Author(s):  
Yi Wang ◽  
Jing Li ◽  
Wanrong Gu ◽  
Qian Zhang ◽  
Lixin Tian ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Low Temperature ◽  
Antioxidant Enzyme ◽  
Temperature Stress ◽  
Aminolevulinic Acid ◽  
Photosynthetic Capacity ◽  
Antioxidant Enzyme Activity ◽  
Low Temperature Stress ◽  
Temperature Sensitive ◽  
Maize Seedlings

The important plant growth regulator 5-aminolevulinic acid (ALA) could promote low-temperature stress tolerance of many plants; however, the underlying mechanisms remain to be elucidated. We investigated the effects of exogenously applied ALA on seedling morphology, antioxidant enzyme activity and photosynthetic capacity of maize (Zea mays L.) seedlings under low-temperature stress. Two cultivars, low-temperature-sensitive cv. Suiyu 13 (SY13) and low-temperature-tolerant cv. Zhengdan 958 (ZD958), were subjected to four treatments: low-temperature without ALA treatment, low-temperature after ALA treatment, normal temperature without ALA treatment, and normal temperature after ALA treatment. Plant morphological growth, proline content, antioxidant enzyme activity and photosynthetic capacity were determined. ALA treatment significantly decreased the inhibitory effects of low-temperature stress on seedling dry weight and increased proline accumulation under low temperatures in ZD958. Pre-application of ALA significantly improved superoxide dismutase and catalase activities in SY13 under low-temperature stress. Furthermore, treating maize seedlings with ALA resulted in significant enhancement of ribulose-1,5-bisphosphate (RuBP) carboxylase activity under low-temperature stress in both cultivars. Pre-treatment with ALA relieved the damage caused by low-temperature stress to maize seedlings, particularly in the low-temperature-sensitive cultivar. Therefore, ALA at appropriate concentrations may be used to prevent reductions in maize crop yield due to low-temperature stress.

Influence of Low-temperature Stress on Photosynthetic Traits in Maize Seedlings

2013 ◽  
Vol 20 (3) ◽  
pp. 1-5 ◽  
Author(s):  
Zhuang Wen-feng ◽  
Wu Xin-juan ◽  
Yang Meng ◽  
Gu Wan-rong ◽  
Xu Zheng-jin ◽  
...  
Keyword(s):  
Low Temperature ◽  
Temperature Stress ◽  
Low Temperature Stress ◽  
Maize Seedlings ◽  
Photosynthetic Traits
Sign in / Sign up

Export Citation Format

Share Document