scholarly journals Microwave Assisted Pretreatment of Szarvasi (Agropyron elongatum) Biomass to Enhance Enzymatic Saccharification and Direct Glucose Production

2022 ◽  
Vol 12 ◽  
Author(s):  
Nicolai D. Jablonowski ◽  
Markus Pauly ◽  
Murali Dama
Keyword(s):  
Plant Biomass ◽  
Enzymatic Saccharification ◽  
Glucose Production ◽  
Renewable Resource ◽  
Enzymatic Digestion ◽  
Acid Pretreatment ◽  
Agropyron Elongatum ◽  
Microwave Assisted ◽  
Marginal Soils ◽  
Saccharification Efficiency

Biomass from perennial plants can be considered a carbon-neutral renewable resource. The tall wheatgrass hybrid Szarvasi-1 (Agropyron elongatum, hereafter referred to as “Szarvasi”) belongs to the perennial Poaceae representing a species, which can grow on marginal soils and produce large amounts of biomass. Several conventional and advanced pretreatment methods have been developed to enhance the saccharification efficiency of plant biomass. Advanced pretreatment methods, such as microwave-assisted pretreatment methods are faster and use less energy compared to conventional pretreatment methods. In this study, we investigated the potential of Szarvasi biomass as a biorefinery feedstock. For this purpose, the lignocellulosic structure of Szarvasi biomass was investigated in detail. In addition, microwave-assisted pretreatments were applied to Szarvasi biomass using different reagents including weak acids and alkali. The produced pulp, hydrolysates, and extracted lignin were quantitatively characterized. In particular, the alkali pretreatment significantly enhanced the saccharification efficiency of the pulp 16-fold compared to untreated biomass of Szarvasi. The acid pretreatment directly converted 25% of the cellulose into glucose without the need of enzymatic digestion. In addition, based on lignin compositional and lignin linkage analysis a lignin chemical model structure present in Szarvasi biomass could be established.

Microwave-assisted acid pretreatment for enhancing enzymatic saccharification of sugarcane trash

2020 ◽  
Author(s):  
Euis Hermiati ◽  
Raden Permana Budi Laksana ◽  
Widya Fatriasari ◽  
Lutfi Nia Kholida ◽  
Ahmad Thontowi ◽  
...  
Keyword(s):  
Enzymatic Saccharification ◽  
Acid Pretreatment ◽  
Microwave Assisted

scholarly journals Improvement of Enzymatic Saccharification of Cellulose-Containing Raw Materials Using Aspergillus niger

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1360
Author(s):  
Ekaterina Budenkova ◽  
Stanislav Sukhikh ◽  
Svetlana Ivanova ◽  
Olga Babich ◽  
Vyacheslav Dolganyuk ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Aspergillus Niger ◽  
Filter Paper ◽  
Raw Materials ◽  
Enzymatic Saccharification ◽  
Wood Chip ◽  
Cellulase Activity ◽  
Wood Chips ◽  
Acid Pretreatment ◽  
Hydrolysis Of

Enzymatic hydrolysis of cellulose-containing raw materials, using Aspergillus niger, were studied. Filter paper, secondary cellulose-containing or starch-containing raw materials, miscanthus cellulose after alkaline or acid pretreatment, and wood chip cellulose, were used as substrates. The study focused on a wild A. niger strain, treated, or not (control), by ultraviolet (UV) irradiations for 45, 60, or 120 min (UV45, UV60, or UV120), or by UV irradiation for 120 min followed by a chemical treatment with NaN3 + ItBr for 30 min or 80 min (UV120 + CH30 or UV120 + CH80). A mixture of all the A. niger strains (MIX) was also tested. A citrate buffer, at 50 mM, wasthe most suitable for enzymatic hydrolysis. As the UV exposure time increased to 2 h, the cellulase activity of the surviving culturewas increased (r = 0.706; p < 0.05). The enzymatic activities of the obtained strains, towards miscanthus cellulose, wood chips, and filter paper, were inferior to those obtained with commercial enzymes (8.6 versus 9.1 IU), in some cases. Under stationary hydrolysis at 37 °C, pH = 4.7, the enzymatic activity of A. niger UV120 + CH30 was 24.9 IU. The enzymatic hydrolysis of secondary raw materials, using treated A. niger strains, was themost effective at 37 °C. Similarly, the most effective treatment of miscanthus cellulose and wood chips occurred at 50 °C. The maximum conversion of cellulose to glucose was observed using miscanthus cellulose (with alkaline pretreatment), and the minimum conversion was observed when using wood chips. The greatest value of cellulase activity was evidenced in the starch-containing raw materials, indicating that A. niger can ferment not only through cellulase activity, but also via an amylolytic one.

scholarly journals Disruption of Oil Palm Empty Fruit Bunches by Microwave-assisted Oxalic Acid Pretreatment

2017 ◽  
Vol 49 (3) ◽  
pp. 244 ◽  
Author(s):  
Nissa Nurfajrin Solihat ◽  
Fahriya Puspita Sari ◽  
Lucky Risanto ◽  
Sita Heris Anita ◽  
Fitria Fitria ◽  
...  
Keyword(s):  
Oxalic Acid ◽  
Oil Palm ◽  
Acid Pretreatment ◽  
Empty Fruit Bunches ◽  
Microwave Assisted

scholarly journals Evaluation of the Interactive Effect Pretreatment Parameters via Three Types of Microwave-Assisted Pretreatment and Enzymatic Hydrolysis on Sugar Yield

Processes ◽  
2020 ◽  
Vol 8 (7) ◽  
pp. 787 ◽  
Author(s):  
Saleem Ethaib ◽  
Rozita Omar ◽  
Mustapa Kamal Siti Mazlina ◽  
Awang Biak Dayang Radiah
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Interactive Effect ◽  
Reducing Sugar ◽  
Sugar Yield ◽  
Solid Loading ◽  
Acid Pretreatment ◽  
Glucose Yield ◽  
Sago Palm ◽  
Microwave Assisted

This study aims to evaluate the sugar yield from enzymatic hydrolysis and the interactive effect pretreatment parameters of microwave-assisted pretreatment on glucose and xylose. Three types of microwave-assisted pretreatments of sago palm bark (SPB) were conducted for enzymatic hydrolysis, namely: microwave-sulphuric acid pretreatment (MSA), microwave-sodium hydroxide pretreatment (MSH), and microwave-sodium bicarbonate (MSB). The experimental design was done using a response surface methodology (RSM) and Box–Behenken Design (BBD). The pretreatment parameters ranged from 5–15% solid loading (SL), 5–15 min of exposure time (ET), and 80–800 W of microwave power (MP). The results indicated that the maximum total reducing sugar was 386 mg/g, obtained by MSA pretreatment. The results also illustrated that the higher glucose yield, 44.3 mg/g, was found using MSH pretreatment, while the higher xylose yield, 43.1 mg/g, resulted from MSA pretreatment. The pretreatment parameters MP, ET, and SL showed different patterns of influence on glucose and xylose yield via enzymatic hydrolysis for MSA, MSH, and MSB pretreatments. The analyses of the interactive effect of the pretreatment parameters MP, ET, and SL on the glucose yield from SPB showed that it increased with the high MP and longer ET, but this was limited by low SL values. However, the analysis of the interactive effect of the pretreatment parameters on xylose yields revealed that MP had the most influence on the xylose yield for MSA, MSH, and MSB pretreatments.

Characterization of Hemp Fiber Using Microwave-Assisted Extraction (MAE) Pretreatment

2010 ◽  
Vol 148-149 ◽  
pp. 1594-1599
Author(s):  
Guo Jun Han ◽  
Li Jun Qu ◽  
Xiao Qing Guo
Keyword(s):  
Low Cost ◽  
Microwave Assisted Extraction ◽  
X Ray Diffraction ◽  
Acid Pretreatment ◽  
Hemp Fiber ◽  
Hemp Fibers ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Short Period ◽  
Index Contrast

The main objective of this paper was to investigate a low cost pretreatment method, namely microwave-assisted extraction(MAE) pretreatment, which could be applied to hemp degumming with low environmental impact. Degumming index, contrast of different pretreatment methods, transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD), and single fiber tensile testing were used to characterise the effect of pretreatment on hemp fibers. In this paper, the results of degumming effect were given comparing MAE pretreatment with acid pretreatment for hemp degumming. It was found that the MAE pretreatment could remove non-cellulosic compounds from hemp bundles effectively with very short period of time. Meanwhile, seen from SEM micrographs, the fibers pretreated by MAE were cleaner than that of fibers pretreated by acid. These results suggested that MAE pretreatment was higher efficiency and more environmental friendly than traditional pretreatment methods.

Short duration microwave assisted pretreatment enhances the enzymatic saccharification and fermentable sugar yield from sugarcane bagasse

2012 ◽  
Vol 37 (1) ◽  
pp. 109-116 ◽  
Author(s):  
Parameswaran Binod ◽  
Karri Satyanagalakshmi ◽  
Raveendran Sindhu ◽  
Kanakambaran Usha Janu ◽  
Rajeev K. Sukumaran ◽  
...  
Keyword(s):  
Short Duration ◽  
Sugarcane Bagasse ◽  
Enzymatic Saccharification ◽  
Sugar Yield ◽  
Fermentable Sugar ◽  
Microwave Assisted
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