scholarly journals First Detection of Listeria monocytogenes in a Buffalo Aborted Foetus in Campania Region (Southern Italy)

2021 ◽  
Vol 7 ◽  
Author(s):  
Claudia Esposito ◽  
Lorena Cardillo ◽  
Giorgia Borriello ◽  
Grazia Ascione ◽  
Ornella Valvini ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Water Buffalo ◽  
Southern Italy ◽  
Bubalus Bubalis ◽  
Reproductive Disorders ◽  
Campania Region ◽  
Molecular Tests ◽  
Food Borne ◽  
Diagnostic Panel ◽  
First Time

Listeria monocytogenes (LM) is the causative agent of listeriosis in both animals and humans, representing one of the most severe food-borne diseases in humans. Out of 13 serotypes, only three (i.e., 1/2a, 1/2b, and 4b) are responsible for 95% of human outbreaks of listeriosis. Ruminants have been hypothesised to represent the main natural reservoir for this pathogen and to be involved in the transmission of Listeria to humans. During pregnancy, listeriosis in ruminants cause various reproductive disorders as well as abortion. However, little is known about abortion due to LM in water buffaloes (Bubalus bubalis). In this study, we report for the first time the detection of LM in a water buffalo foetus in the region of Campania, Italy. Complete necropsy was performed, and samples and swabs from the abomasum, kidneys, liver, lungs, and spleen were collected. Microbiological and molecular analyses were carried out to detect bacterial, viral, and protozoarian abortive pathogens. The results revealed the presence of LM in the liver, lungs, and abomasum, and no other agent was detected. Isolation was confirmed by biochemical and molecular tests. Molecular serotype characterisation was performed, and serogroup IVb was identified. In conclusion, because of the zoonotic implications of our findings, this report highlights the importance of including LM in the diagnostic panel in cases of bubaline abortion.

scholarly journals Sphaceloma coryli: A Reemerging Pathogen Causing Heavy Losses on Hazelnut in Southern Italy

Plant Disease ◽  
2016 ◽  
Vol 100 (3) ◽  
pp. 548-554 ◽  
Author(s):  
M. Minutolo ◽  
B. Nanni ◽  
F. Scala ◽  
D. Alioto
Keyword(s):  
Southern Italy ◽  
Mycelium Growth ◽  
Campania Region ◽  
Disease Symptoms ◽  
Vein Necrosis ◽  
Internal Transcribed Spacer Sequences ◽  
Conidium Germination ◽  
Necrotic Spots ◽  
First Time

In 2013 to 2014, a disease causing severe crop losses was observed in several growing areas of the Campania region in southern Italy. Disease symptoms consisted of necrotic spots on leaves, bracts, and shells along with vein necrosis. Nuts were necrotic or aborted and symptomatic fruit dropped prematurely. A fungus was consistently isolated from symptomatic tissues and morphologically identified as Sphaceloma coryli. The fungus was first reported in France and Italy (Campania) over 30 years ago and was not subsequently documented until 2006 in the Latium region of Italy. When artificially inoculated on healthy hazelnut plants, disease symptoms were reproduced. The teleomorph of S. coryli was recorded for the first time on overwintering tissues of hazelnut trees. It was also induced in vitro. The morphology of asci and ascospores together with phylogenetic analysis based on internal transcribed spacer sequences indicated that the teleomorph is an undescribed species within the genus Elsinoë for which the name of Elsinoë coryli is proposed. Optimum temperatures for ascospore and conidium germination and mycelium growth were determined in vitro. The inhibition effect of some commercial fungicides on S. coryli was also evaluated in vitro.

scholarly journals Detection ofBovine Viral Diarrhea Virusfrom Three Water Buffalo Fetuses (Bubalus Bubalis) in Southern Italy

2009 ◽  
Vol 21 (1) ◽  
pp. 137-140 ◽  
Author(s):  
Alessandra Martucciello ◽  
Gian Mario De Mia ◽  
Monica Giammarioli ◽  
Immacolata De Donato ◽  
Giuseppe Iovane ◽  
...  
Keyword(s):  
Water Buffalo ◽  
Southern Italy ◽  
Bubalus Bubalis ◽  
Viral Diarrhea

scholarly journals Whole-Genome Sequencing-Based Characterization of a Listeria monocytogenes Strain from an Aborted Water Buffalo in Southern Italy

2021 ◽  
Vol 9 (9) ◽  
pp. 1875
Author(s):  
Rubina Paradiso ◽  
Marita Georgia Riccardi ◽  
Bianca Cecere ◽  
Nunzia Riccone ◽  
Roberto Scialla ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Water Buffalo ◽  
Southern Italy ◽  
Antibiotic Resistance Genes ◽  
Epidemiological Studies ◽  
Whole Genome ◽  
Pathogenic Potential

Listeria monocytogenes is a Gram-positive pathogen causing life-threatening infections both in humans and animals. In livestock farms, it can persist for a long time and primarily causes uterine infections and encephalitis in farmed animals. Whole genome sequencing (WGS) is currently becoming the best method for molecular typing of this pathogen due to its high discriminatory power and efficiency of characterization. This study describes the WGS-based characterization of an L. monocytogenes strain from an aborted water buffalo fetus in southern Italy. The strain under study was classified as molecular serogroup IVb, phylogenetic lineage I, MLST sequence type 6, Clonal Complex 6, and cgMLST type CT3331, sublineage 6. Molecular analysis indicated the presence of 61 virulence genes and 4 antibiotic resistance genes. Phylogenetic analysis, including all the publicly available European L. monocytogenes serogroup IVb isolates, indicated that our strain clusterized with all the other CC6 strains and that different CCs were variably distributed within countries and isolation sources. This study contributes to the current understanding of the genetic diversity of L. monocytogenes from animal sources and highlights how the WGS strategy can provide insights into the pathogenic potential of this microorganism, acting as an important tool for epidemiological studies.

scholarly journals Identification of a New Serovar of Salmonella enterica in Mediterranean Buffalo Calves (Bubalus bubalis)

Animals ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 161
Author(s):  
Luisa D’Angelo ◽  
Domenico Vecchio ◽  
Debora Cozza ◽  
Immacolata La Tela ◽  
Maria Rosaria Carullo ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Water Buffalo ◽  
Bubalus Bubalis ◽  
Antimicrobial Treatment ◽  
Multi Drug Resistance ◽  
Severe Dehydration ◽  
Buffalo Calves ◽  
Intestinal Contents ◽  
Severe Gastroenteritis ◽  
First Time

This case report describes for the first-time cases of severe gastroenteritis in water buffalo calves due to a new serovar of Salmonella enterica. The study was carried out on fecal matrix collected from live water buffalo calves that showed profuse diarrhea, severe dehydration and fever, exhibiting a systemic course. Culture and molecular investigations identified the pathogens isolated from intestinal contents as two Salmonella serovars, Salmonella enterica enterica O:35 and a new serovar of Salmonella enterica. The isolates showed multi-drug resistance. Timely diagnosis associated with a targeted antimicrobial treatment were found to be sufficient for the survival and recovery of the infected animals. Herd vaccines prepared from isolated pathogens were used to prevent further deaths of the calves.

GROWING INTEREST IN THE WATER BUFFALO: A SHORT BIBLIOGRAPHIC UPDATE

1996 ◽  
Vol 19 ◽  
pp. 7-15 ◽  
Author(s):  
J. Boyazoglu
Keyword(s):  
Water Buffalo ◽  
Bubalus Bubalis ◽  
Animal Origin ◽  
Human Consumption ◽  
Marginal Lands ◽  
Major Work ◽  
Vice Chancellor ◽  
National University ◽  
Cattle Husbandry ◽  
First Time

When FAO published a major work in 1974 (Ross Cockrill), reviewing for the first time the then existing information relating to the water buffalo (Bubalus bubalis), Sir John Grenfell E Crawford, the Vice Chancellor of the Australian National University, introduced it by unr' rlining that “among all farming livestock which science had neglected, the domestic buffalo served as an outstanding example of genera failure to recognise and exploit its production potential” Since then a world-wide interest has developed in this species, not only as a purveyor of anima protein for human consumption, but also because of a growing interest in the diversification of products of animal origin and the manufacture of typical regiona products as well as a variety of renewed uses of these animals. Furthermore, the strong image linking the buffalo with nature and the environment's ecological equilibrium, particularly in some of the world's more marginal lands, shows this species as one of the most viable alternatives of intensive cattle husbandry systems which are more and more under accusation in an environment-conscious society.

scholarly journals Cytogenetic analysis reveals the swamp status of the indigenous “Bhangor” buffalo population from Tripura state

2021 ◽  
Author(s):  
Karan Veer Singh ◽  
Ramendra Das ◽  
Saket Niranjan ◽  
Monica Sodhi ◽  
R.S. Kataria
Keyword(s):  
Water Buffalo ◽  
Bubalus Bubalis ◽  
Domestic Water ◽  
Karyotypic Analysis ◽  
North East India ◽  
North East ◽  
Brahmaputra Valley ◽  
Swamp Buffaloes ◽  
Cytogenetic Analyses ◽  
First Time

The domestic water buffalo (Bubalus bubalis) are classified into the swamp and riverine. However, their hybrids are also found in some parts of Assam (Brahmaputra Valley) in North-east India. Swamp buffaloes have a typical phenotypic appearance, like the shape of horns, small size, and body-color, etc. This study characterizes the indigenous ‘Bhangor’ buffalo population from the Tripura state using cytogenetic analyses. The blood of buffaloes samples were collected across the state, phenotypically identified as swamp buffaloes were cultured, terminated, and harvested using conventional karyotype protocol to determine the number of chromosomes. We have characterized ‘Bhangor’ an indigenous buffalo population from Tripura state using karyotypic analysis for the first time to confirm that all animals phenotypically identified as swamp buffaloes with 2N=48 chromosomes.

Neosporosis in water buffalo (Bubalus bubalis) in southern Italy

2000 ◽  
Vol 91 (1-2) ◽  
pp. 15-21 ◽  
Author(s):  
A Guarino ◽  
G Fusco ◽  
G Savini ◽  
G Di Francesco ◽  
G Cringoli
Keyword(s):  
Water Buffalo ◽  
Southern Italy ◽  
Bubalus Bubalis

scholarly journals Prevalence of Coccidian Species in the Water Buffalo (Bubalus Bubalis) in the Province of Afyon, Turkey

2008 ◽  
Vol 77 (1) ◽  
pp. 111-116 ◽  
Author(s):  
S. Nalbantoglu ◽  
B. Sari ◽  
H. Cicek ◽  
Z. Karaer
Keyword(s):  
Room Temperature ◽  
Water Buffalo ◽  
Potassium Dichromate ◽  
Bubalus Bubalis ◽  
Sugar Solution ◽  
Water Buffaloes ◽  
Faecal Samples ◽  
Coccidian Species ◽  
First Time ◽  
Study Species

The objective of this study was to identify the coccidian species present in water buffaloes in the vicinity of Afyon. Faecal samples were collected directly from the rectum of a total of 104 water buffaloes, and examined in the laboratory using Sheather's saturated sugar solution. Faecal samples found to contain Eimeria species were mixed with a solution of 2.5% potassium dichromate, left at room temperature in petri dishes until the oocysts sporulated, and then examined in order to identify the species. Eleven different Eimeria species and one Isospora species were identified in 78 (75%) out of the 104 water buffaloes as follows: E. zuernii (55.1%), E. auburnensis (44.9%), E. bovis (44.9%), E. ellipsoidalis (28.2%), E. ankarensis (16.7%), E. subspherica (16.7%), E. alabamensis (11.5%), E. cylindrica (10.3%), E. bareillyi (5.1%), E. canadensis (5.1%), E. brasiliensis (3.8%), and Isospora spp. (46.2%). With this study, species causing coccidiosis in water buffaloes in the province of Afyon were identified for the first time, and Isospora spp. in the water buffalo is reported for the first time.

scholarly journals Behaviour of Listeria monocytogenes and Escherichia coli O157:H7 during the cheese making of traditional raw-milk cheeses from Italian Alps

2015 ◽  
Vol 4 (2) ◽  
Author(s):  
Elena Cosciani-Cunico ◽  
Elena Dalzini ◽  
Stefania Ducoli ◽  
Chiara Sfameni ◽  
Barbara Bertasi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Raw Milk ◽  
Starter Cultures ◽  
Cow Milk ◽  
Italian Alps ◽  
Cheese Making ◽  
E Coli ◽  
Food Borne ◽  
First Time

The behaviour of <em>Listeria monocytogenes</em> and <em>Escherichia coli</em> O157:H7 was studied during the manufacture and ripening of two traditional Italian Alps cheeses. Each cheese type was manufactured in a pilot plan from raw cow milk (without the addition of starter cultures) artificially inoculated with <em>L. monocytogenes</em> and <em>E. coli</em> O157:H7 to a final concentration of about 4 log CFU/mL. The pathogens were enumerated throughout the cheese making and ripening processes to study their behaviour. When the milk was inoculated with 4 Log CFU/mL, the pathogens counts increased in the first time during the manufacturing process and then remained constant, until the end of ripening, or decreased significantly. Results indicate that the environment and nature of food borne pathogens affected the concentration of the bacteria during the manufacturing and ripening process. Thus, the presence of low cells numbers of <em>L. monocytogenes</em> and <em>E. coli</em> O157:H7 in milk destined for the production of raw milk cheeses characterized by a cooking of the curd less than 48°C can constitute a hazard for the consumer.

scholarly journals Complete Genome Sequencing of Eight Brucella abortus Biovar 1 Strains Isolated from Water Buffalo

2018 ◽  
Vol 6 (13) ◽  
Author(s):  
Rubina Paradiso ◽  
Massimiliano Orsini ◽  
Marita Georgia Riccardi ◽  
Bianca Cecere ◽  
Anna Cerrone ◽  
...  
Keyword(s):  
Genome Sequencing ◽  
Brucella Abortus ◽  
Complete Genome ◽  
Water Buffalo ◽  
Reproductive Disorders ◽  
Chronic Infections ◽  
Campania Region ◽  
Content Type ◽  
Water Buffaloes ◽  
Genome Assemblies

ABSTRACT Brucellosis is a zoonotic disease caused by bacteria of the genus Brucella . The disease is endemic in many areas, causing chronic infections responsible for reproductive disorders in infected animals. Here, we present eight complete genome assemblies of eight Brucella abortus strains isolated from water buffaloes farmed in the Campania region.

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