scholarly journals Impact of Packing Density on the Bacterial Community, Fermentation, and In Vitro Digestibility of Whole-Crop Barley Silage

Agriculture ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 672
Author(s):  
Lin Sun ◽  
Na Na ◽  
Xiaomei Li ◽  
Ziqin Li ◽  
Chao Wang ◽  
...  
Keyword(s):  
Packing Density ◽  
Ammonia Nitrogen ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Acid Detergent Fiber ◽  
Fresh Matter ◽  
Fermentation Quality ◽  
Barley Silage

Packing density has a significant influence on the outcome of ensiling forage. In this study, we aim to investigate the effect of packing density on the ensiling properties, microbiome, and in vitro digestibility of barley silages. Barley was ensiled in polyethylene drum silos (30 L) with respective densities of 600, 650, 700, and 750 kg/m3 fresh matter (FM), and stored for 60 days. The bacterial communities, fermentation quality, and in vitro digestibility were analyzed. Fresh barley had a low count of lactic acid bacteria (LAB, 104 cfu/g of FM), and Lactobacillus was nearly undetectable (<1%). Increasing the packing density decreased the pH and the content of ammonia nitrogen (NH3-N), ethanol, neutral detergent fiber (NDF), and acid detergent fiber (ADF) of barley silage (p < 0.05), and increased in vitro digestibility of dry matter, NDF, ADF, and DM recovery (p < 0.05). A higher packing density decreased the abundances of Enterobacter (from 47.4% to 35.4%) and Clostridium (from 13.5% to 3.8%), and increased the abundance of Lactobacillus (from 1.8% to 17.0%). Thus, packing density positively correlated with Lactobacillus (p < 0.05) but negatively correlated with Enterobacter (p < 0.05). The pH and the content of ethanol were positively correlated with Enterobacter (p < 0.05) but negatively correlated with Lactobacillus (p < 0.05). In conclusion, the density of 750 kg/m3 FM resulted in the highest silage quality of the densities tested.

scholarly journals Nutritional Evaluation of Sugarcane Cultivars for Ruminants

2020 ◽  
Vol 8 (2) ◽  
pp. 690
Author(s):  
André Sanches de Avila ◽  
Maximiliane Alavarse Zambom ◽  
Gabriele Larissa Hoelscher ◽  
José Barbosa Duarte Júnior ◽  
Andressa Faccenda ◽  
...  
Keyword(s):  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Acid Detergent Fiber ◽  
Stalk Diameter ◽  
High Fraction ◽  
Biometric Measurements ◽  
Carbohydrate Fractionation ◽  
Stalk Length

The objective of this study was to evaluate the nutritional quality of twenty-nine cultivars of sugarcane. The design was completely randomized, with twenty-nine treatments (cultivars) and three replicates. The evaluations were performed at 540 days after planting during the phenological stage of maturation of sugar cane. Stalk length, number of nodes per plant, internodes length and stalk diameter were evaluated. The stalk samples were crushed, pre-dried and ground for achievement of chemical analysis, carbohydrate fractionation and in vitro digestibility of dry matter (DM), organic matter (OM) and neutral detergent fiber (NDF). Data were analyzed by analysis of variance and posteriorly submitted to Scott-Knott test at 5% probability. Regarding biometric measurements the cultivars differed (P<0.05) for the stalk length, but there was no difference (P>0.05) in the number of nodes as well as in the average internode length and stalk diameter. The content of DM, OM, protein, ether extract, NDF and acid detergent fiber showed differences between the cultivars evaluated (P<0.05). Carbohydrate fractionation also differed between cultivars (P <0.05), being that cultivars RB86 7515, RB92 806 and RB03 6091 presented high fraction of rapidly degradable carbohydrates. The highest values of in vitro digestibility of DM and OM occurred for cultivars RB86 7515, RB92 579 and RB03 6091 (P<0.05). Considering the variables analyzed, the cultivars RB03 6091, RB92 579 and RB86 7515 showed greater concentration of rapidly degradable carbohydrates and greater in vitro digestibility of DM and OM demonstrating potential for use in ruminant feeding.

scholarly journals Effects of Adding Pre-Fermented Fluid Prepared from Red Clover or Lucerne on Fermentation Quality and in Vitro Digestibility of Red Clover and Lucerne Silages

Agriculture ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 454
Author(s):  
Lin Sun ◽  
Yun Jiang ◽  
Qinyin Ling ◽  
Na Na ◽  
Haiwen Xu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Dry Matter ◽  
Red Clover ◽  
Ammonia Nitrogen ◽  
In Vitro Digestibility ◽  
Fermentation Quality ◽  
Fermentation Parameters ◽  
Positive Effect

This study examined the effects of chopping or chopping + blender maceration of red clover or lucerne on pre-fermented juice (PFJ) and determined the effects of PFJs on the quality of red clover silage or lucerne silage. The PFJs from chopping red clover (PFJ-RC) or lucerne (PFJ-LC) had a higher lactic acid bacteria (LAB) count than that from chopping + blender maceration (p < 0.05) and were used as additives. Compared with the Control of both silages, adding PFJ increased LAB, lactic acid (LA), and in vitro digestibility of dry matter (IVDMD) (p < 0.05), while pH, acetic acid (AA), and ammonia nitrogen/total nitrogen (NH3-N/TN) were decreased (p < 0.05). For red clover silages, the PFJ-RC treatment contained the greatest LAB and LA and the lowest pH and NH3-N/TN among treatments (p < 0.05); similar results were observed in PFJ-LC treatment for lucerne silages (p < 0.05). The IVDMD of both silages correlated negatively with pH, AA, and NH3-N/TN and positively with LA (p < 0.05). Overall, chopping alone was a better method for preparing PFJ. Adding PFJ at ensiling increased LA and decreased the pH, AA, and NH3-N/TN of both silages. Ensiling lucerne or red clover with PFJ from the ensiling material had a more positive effect on the fermentation parameters mentioned above. Satisfactory fermentation parameters detected in the present study contributed to improving the IVDMD of both silages.

scholarly journals PSXI-35 Effects of cultivation stage and supplementation level of sprouted barley on nutrient content and fermentation quality of fermented diet

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 402-403
Author(s):  
Hyuk jun lee ◽  
Young Ho Joo ◽  
Seong Shin Lee ◽  
Jong Ho Park ◽  
Tae Il Kim ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Nutrient Content ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
Total Mixed Ration ◽  
Microbial Counts ◽  
Fermentation Quality

Abstract This study was aimed to estimate the effect of cultivation stage and supplementation level of sprouted barley (SB) on nutrient content and fermentation quality of fermented total mixed ration (TMR). The SB were collected at day 6 (SB6) and day 8 (SB8) of cultivation stages and substituted at 5% (TMR5) and 10% (TMR10) of TMR on dry matter (DM) basis. These TMR were ensiled into 10 L mini silo (3 kg) for 14 days in triplicate. After silo open, sample (1 kg) was collected for laboratory analysis. Before ensiling, crude protein (CP) was lower (P = 0.012) in SB6 than in SB8. Additionally, DM in TMR10 was slightly lower (P = 0.015) than in TMR5 while CP and acid detergent fiber were higher (P &lt; 0.05). After fermentation, crude ash (P = 0.002), in vitro DM digestibility (IVDMD) (P = 0.001; 73.5 vs. 72.0%) and in vitro neutral detergent fiber (IVNDFD) (P = 0.001; 50.6 vs. 47.7%) were higher in SB6 than in SB8. Additionally, DM (P = 0.002; 64.0 vs. 64.5) in TMR10 was lower than in TMR5, while CP (P = 0.005; 19.7 vs. 18.9%), IVDMD (P = 0.001; 74.5 vs. 71.0%), and IVNDFD (P = 0.001; 52.0 vs. 46.3%) were higher. Fermentation indices and microbial counts were not affected by cultivation stage of SB, except on propionate that higher (P = 0.014) in SB8 than SB6. Silage pH (P = 0.001) was lower in TMR10 than in TMR5, which followed by higher lactate (P = 0.003; 3.27 vs. 2.85%), acetate (P = 0.004; 0.52 vs. 0.42%), propionate (P &lt; 0.001), and LAB count (P = 0.020; 7.49 vs. 7.19%). The interaction effects between cultivation stage and supplementation level only found on propionate production (P = 0.001). Yeast and mold were not affected by cultivation stage and supplementation level of SB. This study concluded SB6 was recommended to improve fermentation quality of fermented diet, and its application on TMR10 could increase not only rumen digestibility but also fermentation indices.

scholarly journals Utilization of a novel feedstuff: effects of additives on the fermentation characteristics, chemical composition and in vitro digestibility of tetraploid black locust (Robinia pseudoacacia) silage

2019 ◽  
Vol 49 (7) ◽  
Author(s):  
Zhihao Dong ◽  
Junfeng Li ◽  
Lei Chen ◽  
Siran Wang ◽  
Tao Shao
Keyword(s):  
Lactic Acid ◽  
Chemical Composition ◽  
Lactobacillus Plantarum ◽  
Dry Matter ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Fermentation Products ◽  
Fermentation Quality ◽  
Tetraploid Black Locust

ABSTRACT: This study was conducted to evaluate the effects of additives on the fermentation characteristics, chemical composition and in vitro digestibility of tetraploid black locust (TBL). The TBL leaves silage was either untreated (control) or treated with 1 × 106 cfu/g FM Lactobacillus plantarum (L), 1% glucose (G), 3% molasses (M), a combination of 1% glucose and Lactobacillus plantarum (L+G), or a combination of 3% molasses and Lactobacillus plantarum (L+M). Fermentation quality, chemical composition and nutrient digestibility were then analyzed. Ethanol and acetic acid concentrations were the dominant fermentation products in all silages except L+M silage. The L, G and L+G treatments failed to influence the fermentation. The M treatment increased (P<0.05) the lactic acid concentration and lowered (P<0.05) the pH when compared with control silage. The best fermentation properties were observed in L+M silage, as indicated by the dominance of lactic acid over ethanol in fermentation products. The M and L+M silages exhibited higher (P<0.05) dry matter, and M silage showed higher residual water-soluble carbohydrates than the control. Ensiling increased (P<0.05) the in vitro dry matter, neutral detergent fiber and acid detergent fiber degradability of TBL. Among the silages, M silage had the highest levels of dry matter, neutral detergent fiber and acid detergent fiber degradability. The obtained results suggested that application of lactic acid bacteria together with 3% molasses could be an effective strategy to prevent the occurrence of ethanol fermentation and improve fermentation quality of TBL silage; addition of fermentable sugars to TBL improves nutrient availability to ruminants.

EVALUATION OF PINEGRASS AS RANGE FORAGE

1969 ◽  
Vol 49 (3) ◽  
pp. 351-359 ◽  
Author(s):  
Alastair McLean ◽  
S. Freyman ◽  
J. E. Miltimore ◽  
D. M. Bowden
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Soluble Carbohydrate ◽  
In Vitro Digestibility ◽  
Native Forest ◽  
Acid Detergent Fiber ◽  
High Positive Correlation ◽  
Calcium Iron

Seasonal changes in the quality of pinegrass (Calamagrostis rubescens) on native forest range in southern British Columbia included declines in crude protein content and in vitro digestibility of dry matter and increases in content of lignin, acid-detergent fiber, and ash. The decline in average daily gains of beef heifers was more rapid than changes in quality of the pinegrass over the grazing period from June to October.Pinegrass contained adequate nutrients for the rapid growth of yearlings or for maintaining weanling calves until August 1, whereupon protein and phosphorus supplementation were required. Nutrient requirements of pregnant cows and replacement heifers were adequately met until September 1.Copper and zinc were inadequate throughout the season. Calcium, iron, and manganese contents, however, were satisfactory. Silica was high and must be considered a potential problem in pinegrass utilization.Digestible dry matter gave a high positive correlation with crude protein and phosphorus and a high negative correlation with lignin, acid-detergent fiber, and total ash.Crude fiber, crude fat, and total soluble carbohydrate contents of pinegrass did not closely follow changes in either animal gains or in vitro digestible dry matter.Crude protein, phosphorus, and acid-detergent fiber appear to give a good indication of animal yields on pinegrass and could be used to evaluate pinegrass quality where animal data are not available.

Fermentation quality, in vitro digestibility and aerobic stability of total mixed ration silages prepared with whole-plant corn (Zea mays L.) and hulless barley (Hordeum vulgare L.) straw

2018 ◽  
Vol 58 (10) ◽  
pp. 1860 ◽  
Author(s):  
XianJun Yuan ◽  
AiYou Wen ◽  
Jian Wang ◽  
JunFeng Li ◽  
Seare T. Desta ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Ammonia Nitrogen ◽  
Stability Test ◽  
Small Scale ◽  
In Vitro Digestibility ◽  
Total Mixed Ration ◽  
Aerobic Stability ◽  
Fermentation Quality

This study was carried out to assess the effects of adding Lactobacillus plantarum, molasses or/and ethanol on the fermentation quality, in vitro digestibility and aerobic stability of total mixed ration (TMR) silage, which is well accepted in small-scale dairy farms in Tibet. Total mixed ration were ensiled in laboratory silos (1 L) and treated with (1) no additive (Control), (2) ethanol (E, 25 ml/kg fresh weight (FW)), (3) molasses (M, 30 g/kg FW); (4) Lactobacillus plantarum (L, 106cfu/g FW); (5) ethanol + molasses (EM); and (6) ethanol + Lactobacillus plantarum (EL). After 45 days of ensiling, six silos per treatment were opened for the fermentation quality and in vitro digestibility analyses, whereas 18 silos were used for the aerobic stability test for the following 9 days. All TMR silages were well preserved with dominant lactic acid (LA), low pH and ammonia nitrogen, and negligible propionic and butyric acid. The L and EL silages had the lowest pH and highest LA concentrations. The addition of ethanol did not inhibit silage fermentation as there were no significant differences for the pH, LA, acetic acid, negligible propionic acid or ammonia nitrogen content, lactic acid bacteria and yeast counts between Control and the E silage. During the aerobic stability test, pH increased by 1.39, 1.67, 1.69 and 0.74 for the Control, M, L and EM silages, but only 0.40 and 0.34 for E and EL silages, respectively. Upon exposure to air, the LA concentration in the L silage was evidently (P < 0.05) decreased, whereas LA concentration in the EL silage remained the highest value after the third day of aerobic exposure. Mean populations of aerobic bacteria and yeast in the E and EL silages were lower (P < 0.05) than those of the Control. These findings suggested that L. plantarum is effective in improving fermentation quality of TMR silages. Although the addition of ethanol in our study did not depress the fermentation of the TMR silages, it showed potential to inhibit the aerobic spoilage of TMR silages, either alone or in combination with the L. plantarum. It is concluded that L. plantarum combined with ethanol not only ensures better fermentation but also could improve aerobic stability.

scholarly journals In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4507
Author(s):  
Luiz Juliano Valério Geron ◽  
Fabiana Gomes da Costa ◽  
Silvia Cristina de Aguiar ◽  
Jocilaine Garcia ◽  
Matheus Gonçalves Ribeiro ◽  
...  
Keyword(s):  
Crude Protein ◽  
Ammonia Nitrogen ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
Ruminal Fluid ◽  
Ph Values

<p>This study evaluated the digestibility of nutrients by, and parameters associated with, <em>in vitro </em>fermentation using different inocula (sheep ruminal fluid and feces) as well as the <em>in vivo </em>digestibility in sheep that were fed rations with 50% concentrate containing either no (0%) residue from the extraction of tamarind pulp (RETP) or 15% RETP. To determine the <em>in vitro </em>digestibility (<em>IV</em>D) of nutrients, two sheep, weighing 40.38 ± 2.10 kg, were used as inoculum donors. To determine the <em>in vivo </em>digestibility of nutrients, we used four sheep and a 3×2 factorial experimental design, with three methods of digestion of nutrients and two experimental rations (0% and 15% RETP). The variables were subjected to analysis of variance and the variables that showed differences at 5% probability were further analyzed using the Tukey test at 5% significance. The <em>IV</em>D using different inocula did not significantly differ (p&gt;0.05) from the <em>in vivo </em>digestibility in sheep for dry matter (DM), organic matter (OM), and neutral detergent fiber (NDF). The different methods for determining nutrient digestibility did not affect (p&gt;0.05) the digestibility of DM, OM, crude protein (CP), NDF, and acid detergent fiber (ADF) in rations with 0% and 15% RETP. However, the <em>IV</em>D of CP for rations containing 0% and 15% RETP incubated with both inocula was lower (p&lt;0.05) than the CP digestibility <em>in vivo</em>. The <em>in vivo </em>digestibility of ADF for rations containing 0% and 15% RETP was higher (P&lt;0.05) than the <em>IV</em>D using sheep ruminal fluid and feces as inocula. The pH values and concentration of ammonia nitrogen (NH3-N) after <em>in vitro </em>incubation for 24 h and the <em>in vivo </em>assay were not different (p&gt;0.05) for the rations containing 0% and 15% RETP, but the pH and NH3-N of both fermented and rumen contents differed (p&lt;0.05) depending on the inocula used and the <em>in vivo </em>assay. In summary, the digestibility of DM, OM, and NDF can be determined by the <em>in vitro </em>fermentation method using the ruminal fluid or feces of sheep as inocula in rations containing 0% or 15% RETP. However, <em>in vitro </em>fermentation is not a suitable method for the determination of pH and NH3-N concentration.</p>

scholarly journals Mixed sugarcane and elephant grass silages with or without bacterial inoculant

2010 ◽  
Vol 39 (3) ◽  
pp. 462-470 ◽  
Author(s):  
Jucilene Cavali ◽  
Odilon Gomes Pereira ◽  
Sebastião de Campos Valadares Filho ◽  
Marlos Oliveira Porto ◽  
Francisco Eden Paiva Fernandes ◽  
...  
Keyword(s):  
Dry Matter ◽  
Linear Models ◽  
Water Soluble ◽  
Neutral Detergent Fiber ◽  
Soluble Carbohydrate ◽  
In Vitro Digestibility ◽  
Elephant Grass ◽  
Acid Detergent Fiber ◽  
Total N

The effects of different ratios of sugarcane and elephant grass (0:100, 25:75, 50:50, 75:25 and 100:0% of the natural basis) were assessed on the chemical composition and losses in silages treated with a bacterial inoculant, using laboratory silos. A 2 × 5 factorial arrangement (with and without inoculant and five elephant grass ratios) in a randomized blocks design with three replications was used. Interaction was observed in the sugarcane and elephant grass ratio × bacterial inoculant for crude protein (CP) and pH. The other variables were influenced only by the increasing proportions of elephant grass. The contents of dry matter, neutral detergent fiber corrected for ashes and protein, acid detergent fiber, insoluble protein in acid detergent fiber, hemicellulose and lignin in the silages increased linearly with the proportions of elephant grass. The water soluble carbohydrate contents and dry matter in vitro digestibility of the silages decreased linearly with the increase in the proportion of elephant grass. The mean value of ammonia nitrogen in relation to total N was 7.0% (% of DM). The CP values of the inoculated and non-inoculated silage fitted linear models. The highest CP content was observed in the silage treated with inoculant. The pH values of the silages, with and without inoculant, fitted quadratic and linear models, respectively. The lactic acid, propionic acid and butyric acid contents were not influenced by the elephant grass ratios, while the acetic acid content, for the non-inoculated silages, and ethanol decreased linearly with the increase in elephant grass. The ratio of 25% sugarcane and 75% elephant grass improves the nutritional value and increases the silage DM recovery, because of the lower effluent and gas productions. The homofermentative bacterial inoculant does not affect the sugarcane silage.

scholarly journals Investigating the Quality of Commercial Beef Cattle Feeds and Feed Ingredients Used in Bangladesh

2020 ◽  
Vol 18 (1) ◽  
pp. 197-208
Author(s):  
MT Kamal ◽  
MA Hashem ◽  
M Al Mamun ◽  
MM Hossain ◽  
MA Razzaque ◽  
...  
Keyword(s):  
Chemical Analysis ◽  
Beef Cattle ◽  
Neutral Detergent Fiber ◽  
Control Measures ◽  
In Vitro Digestibility ◽  
Feed Ingredients ◽  
Commercial Feeds ◽  
Feed Samples

The study was undertaken to evaluate the quality of commercial beef cattle feed and feed ingredients which are available in Bangladesh. For this purpose, chemical analysis and in vitro digestibility (IVD) were estimated for nine commercial feeds and different feed ingredients which were collected from different regions. Chemical analysis of samples was carried out in triplicate for dry matter (DM), crude protein (CP), crude fiber (CF), ether extract (EE), ash, acid detergent fiber (ADF) and neutral detergent fiber (NDF) content. Metabolizable energy (ME) was calculated mathematically for feed samples by using standard formula. The analysis revealed the difference between the manufacturer’s claim and actual analyzed value. In commercial feeds DM content ranged from 90-92%. While CP content of commercial feeds was either lower or higher than the written value of feed industries. In Provita feed CP content (20.72%) was higher and Care feed had much lower CP content (7.54%) than written value. In vitro digestibility of DM in feed ingredients varied from 18.27 in straw to 75.77% in soybean meal. The fiber component (NDF and ADF) was negatively correlated with IVDMD and CP was positively correlated with IVDMD because fiber rich components were less digestible than the non-fibrous component (protein). Analysis of commercial feed samples revealed that the values claimed by the manufacturers are quite different from the actual analyzed values. This is a matter of concern and needs to be checked through better quality control measures by systematic feed analysis and ensure that manufacturers declare the true composition of the marketed feeds. SAARC J. Agri., 18(1): 197-208 (2020)

scholarly journals Elephant grass silages with or without wilting, with cassava meal in silage production

2017 ◽  
Vol 18 (3) ◽  
pp. 417-429 ◽  
Author(s):  
Aline Cardoso OLIVEIRA ◽  
Rasmo GARCIA ◽  
Aureliano José Vieira PIRES ◽  
Hellenn Cardoso OLIVEIRA ◽  
Vitor Visintin Silva de ALMEIDA ◽  
...  
Keyword(s):  
Nutritive Value ◽  
Free Acid ◽  
Organic Matter Content ◽  
Ammonia Nitrogen ◽  
Matter Content ◽  
Neutral Detergent Fiber ◽  
In Vitro Digestibility ◽  
Elephant Grass ◽  
Linear Effect

SUMMARY The experiment was developed to evaluate the effects of wilting and inclusion of cassava meal level on nutritive value, fermentative characteristics and losses of elephant grass silage. The 4 x 2 factorial scheme was adopted, with four repetitions, in a completely randomized design. The factors evaluated were wilting (without and with) and cassava meal level (0; 7.5; 15 and 22.5% of natural matter, DN). PVC silos with 50 cm height and 10 cm diameter were used for silages production, which were opened after 60 days. The wilting and cassava meal addition had increasing linear effect on DM content. Silages with cassava meal showed reduction of total nitrogen, ether extract, acid detergent insoluble nitrogen, neutral detergent fiber ash and protein free, acid detergent fiber, celluloses, hemicelluloses and lignin contents and increase of organic matter content, DM in vitro digestibility and total digestible nutrients. Relative to variables inherent to silages’ fermentative characteristics, pH presented quadratic behavior. Concerning to ammonia nitrogen, it was observed quadratic and decreasing linear behavior for silages without and with wilting, respectively. Wilting and cassava meal inclusion provided smaller effluent losses.

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