scholarly journals Phone App to Perform Quality Control of Pesticide Spray Applications in Field Crops

Agriculture ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 916
Author(s):  
Christian Nansen ◽  
Gabriel Del Villar ◽  
Alexander Recalde ◽  
Elvis Alvarado ◽  
Krishna Chennapragada

It has been recognized for decades that low and inconsistent spray coverages of pesticide applications represent a major challenge to successful and sustainable crop protection. Deployment of water-sensitive spray cards combined with image analysis can provide valuable and quantitative insight into spray coverage. Herein we provide description of a novel and freely available smartphone app, “Smart Spray”, for both iOS and Android smart devices (iOS and Google app stores). More specifically, we provide a theoretical description of spray coverage, and we describe how Smart Spray and similar image-processing software packages can be used as decision support tools and quality control for pesticide spray applications. Performance assessment of the underlying pixel classification algorithm is presented, and we detail practical recommendations on how to use Smart Spray to maximize accuracy and consistency of spray coverage predictions. Smart Spray was developed as part of ongoing efforts to: (1) maximize the performance of pesticide sprays, (2) minimize pest-induced yield loss and to potentially reduce the amount of pesticide used, (2) reduce the risk of target pests developing pesticide resistance, (3) reduce the risk of spray drift, and (4) optimize spray application costs by introducing a quality control.

2020 ◽  
Vol 21 (3) ◽  
pp. 179-193
Author(s):  
Chatterjee Anupriya ◽  
Nirwan Shradha ◽  
Bandyopadhyay Prasun ◽  
Agnihotri Abha ◽  
Sharma Pankaj ◽  
...  

: Oilseed brassicas stand as the second most valuable source of vegetable oil and the third most traded one across the globe. However, the yield can be severely affected by infections caused by phytopathogens. White rust is a major oomycete disease of oilseed brassicas resulting in up to 60% yield loss globally. So far, success in the development of oomycete resistant Brassicas through conventional breeding has been limited. Hence, there is an imperative need to blend conventional and frontier biotechnological means to breed for improved crop protection and yield. : This review provides a deep insight into the white rust disease and explains the oomycete-plant molecular events with special reference to Albugo candida describing the role of effector molecules, A. candida secretome, and disease response mechanism along with nucleotide-binding leucine-rich repeat receptor (NLR) signaling. Based on these facts, we further discussed the recent progress and future scopes of genomic approaches to transfer white rust resistance in the susceptible varieties of oilseed brassicas, while elucidating the role of resistance and susceptibility genes. Novel genomic technologies have been widely used in crop sustainability by deploying resistance in the host. Enrichment of NLR repertoire, over-expression of R genes, silencing of avirulent and disease susceptibility genes through RNA interference and CRSPR-Cas are technologies which have been successfully applied against pathogen-resistance mechanism. The article provides new insight into Albugo and Brassica genomics which could be useful for producing high yielding and WR resistant oilseed cultivars across the globe.


Author(s):  
Mangal Shailesh Nagarsenker ◽  
Megha Sunil Marwah

The science of liposomes has expanded in ambit from bench to clinic through industrial production in thirty years since the naissance of the concept. This chapter makes an attempt to bring to light the impregnable contributions of great researchers in the field of liposomology that has witnessed clinical success in the recent times. The journey which began in 1965 with the observations of Bangham and further advances made en route (targeting/stealthing of liposomes) along with alternative and potential liposome forming amphiphiles has been highlighted in this chapter. The authors have also summarised the conventional and novel industrially feasible methods used to formulate liposomes in addition to characterisation techniques which have been used to set up quality control standards for large scale production. Besides, the authors have provided with an overview of primary therapeutic and diagnostic applications and a brief insight into the in vivo behaviour of liposomes.


2020 ◽  
Vol 117 (8) ◽  
pp. 4099-4108 ◽  
Author(s):  
Débora Broch Trentini ◽  
Matteo Pecoraro ◽  
Shivani Tiwary ◽  
Jürgen Cox ◽  
Matthias Mann ◽  
...  

Mammalian cells present a fingerprint of their proteome to the adaptive immune system through the display of endogenous peptides on MHC-I complexes. MHC-I−bound peptides originate from protein degradation by the proteasome, suggesting that stably folded, long-lived proteins could evade monitoring. Here, we investigate the role in antigen presentation of the ribosome-associated quality control (RQC) pathway for the degradation of nascent polypeptides that are encoded by defective messenger RNAs and undergo stalling at the ribosome during translation. We find that degradation of model proteins by RQC results in efficient MHC-I presentation, independent of their intrinsic folding properties. Quantitative profiling of MHC-I peptides in wild-type and RQC-deficient cells by mass spectrometry showed that RQC substantially contributes to the composition of the immunopeptidome. Our results also identify endogenous substrates of the RQC pathway in human cells and provide insight into common principles causing ribosome stalling under physiological conditions.


Geosciences ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 231 ◽  
Author(s):  
Andrea Nardini ◽  
Santiago Yépez ◽  
Maria Dolores Bejarano

This paper presents a systematic procedure for developing a characterization and classification of river reaches inspired by the River Styles Framework, through which insight can be gained about the understanding of river behavior. Our procedure takes advantage of several computer based “tools”, i.e., algorithms implemented in software packages of various types, from “simple” Excel sheets to sophisticated algorithms in Python language, in general all supported by Geographic Information Systems (GIS). The main potentially useful, existing tools for this specific aim are discussed here, revealing their strengths and weaknesses. New, complementary or alternative tools that have been developed in the project feeding this paper are presented, which can contribute to the scientific community and stakeholders of the topic. The main result of our research is a structured and practical guide (a ToolBox Manual) that can support practitioners and researchers wishing to characterize and classify large rivers, based on the River Styles Framework. The main contribution is that this set of ideas, solutions, and tools, makes this type of exercise significantly more transparent and at the same time much less subjective. Moreover, the procedure is applicable to large systems and does not require more information than that generally available also in developing or emerging countries.


2020 ◽  
Vol 14 (S342) ◽  
pp. 9-12 ◽  
Author(s):  
Ziri Younsi ◽  
Oliver Porth ◽  
Yosuke Mizuno ◽  
Christian M. Fromm ◽  
Hector Olivares

AbstractUpcoming VLBI observations will resolve nearby supermassive black holes, most notably Sagittarius A* and M87, on event horizon-scales. Recent observations of Sagittarius A* with the Event Horizon Telescope have revealed horizon-scale structure. Accordingly, the detection and measurement of the back hole “shadow” is expected to enable the existence of astrophysical black holes to be verified directly. Although the theoretical description of the shadow is straightforward, its observational appearance is largely determined by the properties of the surrounding accretion flow, which is highly turbulent. We introduce a new polarised general-relativistic radiative transfer code, BHOSS, which accurately solves the equations of polarised radiative transfer in arbitrary strong-gravity environments, providing physically-realistic images of astrophysical black holes on event horizon-scales, as well as also providing insight into the fundamental properties and nature of the surrounding accretion flow environment.


2018 ◽  
Vol 64 ◽  
Author(s):  
N.V. Bespalova ◽  
M.M. Gomola ◽  
T.I. Yakovleva

The article is devoted to the urgent theoretical and practical problems of the quality control of the chocolate confectionery products in cafes and restaurants. The authors analyze the modern approaches to the quality control of the chocolate confectionery products. The practical recommendations can be useful for the process of selection of the high quality raw materials. The criteria for the quality control of the chocolate confectionery products have been given. Some ways to improve the quality of he chocolate confectionery products have been discussed.


Author(s):  
D. Sholdarov ◽  

The article examines the need for a treasury information system in public finance management in the digital economy and the importance of using software packages in the execution of the treasury budget. The analysis of off-budget funds on the accounts of higher educational institutions of the Republic of Uzbekistan and funds received from the contract form of education is carried out. The treasury system provides scientific proposals and practical recommendations for improving the accounting of funds received from payment contracts in higher education institutions.


2019 ◽  
Vol 22 ◽  
Author(s):  
Carla de Oliveira Rosas ◽  
Janaína Marques Rodrigues ◽  
Marcus Henrique Campino de la Cruz ◽  
Silvia Maria dos Reis Lopes ◽  
Aline da Silva Soares Souto ◽  
...  

Abstract Reference materials are crucial for laboratory quality assurance. They are widely used for the internal quality control of analytical tests, in the validation of methodologies and as test items in proficiency tests. Microbiological reference materials are represented mainly by reference strains, whose use in laboratory internal quality control is unquestionable and recommended by several microbiological manuals. In Brazil, the practice of producing microbiological reference materials has advanced in recent years in the development of specific materials for proficiency testing. However, the same did not occur with the establishment of reference strains due to a lack of incentive policies, maintaining the country dependant on the use of international reference strains. This article aims to emphasize the importance of the use of microbiological reference materials in laboratory quality control and discuss the need for the development of such materials in Brazil. The paper presents a brief explanation of microbiological reference materials and points out questions concerning the country’s dependence on the acquisition of international biological materials. It also describes quality standards related to the production of these materials and the situation of culture collections and Brazilian reference laboratories that supply reference strains. The study also mentions practical recommendations on the subcultures of reference strains.


2020 ◽  
Vol 86 (12) ◽  
Author(s):  
Javier Caballero ◽  
Nerea Jiménez-Moreno ◽  
Irene Orera ◽  
Trevor Williams ◽  
Ana B. Fernández ◽  
...  

ABSTRACT Bacillus thuringiensis (Bt) is the most widely used active ingredient for biological insecticides. The composition of δ-endotoxins (Cry and Cyt proteins) in the parasporal crystal determines the toxicity profile of each Bt strain. However, a reliable method for their identification and quantification has not been available, due to the high sequence identity of the genes that encode the δ-endotoxins and the toxins themselves. Here, we have developed an accurate and reproducible mass spectrometry-based method (liquid chromatography-tandem mass spectrometry-multiple reaction monitoring [LC-MS/MS-MRM]) using isotopically labeled proteotypic peptides for each protein in a particular mixture to determine the relative proportion of each δ-endotoxin within the crystal. To validate the method, artificial mixtures containing Cry1Aa, Cry2Aa, and Cry6Aa were analyzed. Determination of the relative abundance of proteins (in molarity) with our method was in good agreement with the expected values. This method was then applied to the most common commercial Bt-based products, DiPel DF, XenTari GD, VectoBac 12S, and Novodor, in which between three and six δ-endotoxins were identified and quantified in each product. This novel approach is of great value for the characterization of Bt-based products, not only providing information on host range, but also for monitoring industrial crystal production and quality control and product registration for Bt-based insecticides. IMPORTANCE Bacillus thuringiensis (Bt)-based biological insecticides are used extensively to control insect pests and vectors of human diseases. Bt-based products provide greater specificity and biosafety than broad-spectrum synthetic insecticides. The biological activity of this bacterium resides in spores and crystals comprising complex mixtures of toxic proteins. We developed and validated a fast, accurate, and reproducible method for quantitative determination of the crystal components of Bt-based products. This method will find clear applications in the improvement of various aspects of the industrial production process of Bt. An important aspect of the production of Bt-based insecticides is its quality control. By specifically quantifying the relative proportion of each of the toxins that make up the crystal, our method represents the most consistent and repeatable evaluation procedure in the quality control of different batches produced in successive fermentations. This method can also contribute to the design of specific culture media and fermentation conditions that optimize Bt crystal composition across a range of Bt strains that target different pestiferous insects. Quantitative information on crystal composition should also prove valuable to phytosanitary product registration authorities that oversee the safety and efficacy of crop protection products.


2008 ◽  
Vol 5 (2) ◽  
pp. 451-462 ◽  
Author(s):  
M. Mauder ◽  
T. Foken ◽  
R. Clement ◽  
J. A. Elbers ◽  
W. Eugster ◽  
...  

Abstract. As part of the quality assurance and quality control activities within the CarboEurope-IP network, a comparison of eddy-covariance software was conducted. For four five-day datasets, CO2 flux estimates were calculated by seven commonly used software packages to assess the uncertainty of CO2 flux estimates due to differences in post-processing. The datasets originated from different sites representing different commonly applied instrumentation and different canopy structures to cover a wide range of realistic conditions. Data preparation, coordinate rotation and the implementation of the correction for high frequency spectral losses were identified as crucial processing steps leading to significant discrepancies in the CO2 flux results. The overall comparison indicated a good although not yet perfect agreement among the different software within 5–10% difference for 30-min CO2 flux values. Conceptually different ideas about the selection and application of processing steps were a main reason for the differences in the CO2 flux estimates observed. A balance should be aspired between scientific freedom on the one hand, in order to advance methodical issues, and standardisation of procedures on the other hand, in order to obtain comparable fluxes for multi-site synthesis studies.


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